FastQCFastQC Report
Fri 27 May 2016
SRR939418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867806
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297611.037761968557148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255210.8899137528828659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191630.6682111690958176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95420.3327282249915092No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63830.2225743303417316No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58430.20374460476057307No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46560.16235407834421156No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42070.1466975102221001No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.10223843593325349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202100.034.4397131
TATAACG1657.6397555E-1131.645882
GTATCAA294250.029.6404341
GTACATG226350.023.9329931
TATCACG1801.9655636E-723.734412
TATCAAC370750.023.4943472
TACATGG230400.023.1781352
ATCAACG376650.023.0852873
TCAACGC380400.022.7735544
CAACGCA385500.022.4848755
AACGCAG393100.022.074296
ACATGGG231550.021.870943
GAGTACT224850.021.79154812-13
AGTACTT226800.021.31153312-13
GTACTTT234300.020.96321914-15
GTACACG5600.020.3476891
CATGGGG75200.019.8838834
ACGCAGA442450.019.5157137
TACTTTT248400.019.47744214-15
CGCAGAG443550.019.4459328
Produced by FastQC (version 0.11.5)