##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2867806 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.881345879044815 31.0 31.0 31.0 28.0 34.0 2 31.111977239743553 31.0 31.0 34.0 28.0 34.0 3 31.088461702081663 31.0 31.0 34.0 28.0 34.0 4 35.01691327795534 35.0 35.0 37.0 32.0 37.0 5 34.594897632545575 35.0 35.0 37.0 30.0 37.0 6 34.66538357197105 35.0 35.0 37.0 31.0 37.0 7 34.50861076376854 35.0 35.0 37.0 30.0 37.0 8 34.40928361262931 35.0 35.0 37.0 30.0 37.0 9 35.866668456652924 37.0 35.0 39.0 30.0 39.0 10-11 35.67002579672405 37.0 35.0 39.0 30.0 39.0 12-13 35.64417886007631 37.0 35.0 39.0 30.0 39.0 14-15 36.38376793967235 38.0 34.5 40.0 30.0 41.0 16-17 36.32939675835813 38.0 34.5 40.0 30.0 41.0 18-19 36.4100758558982 38.0 35.0 40.0 30.0 41.0 20-21 36.33326748741024 38.0 34.5 40.0 29.5 41.0 22-23 36.17174453223126 38.0 34.0 40.0 29.0 41.0 24-25 35.981611726874135 38.0 34.0 40.0 29.0 41.0 26-27 35.48556771273928 38.0 34.0 40.0 27.0 41.0 28-29 35.25478850382487 38.0 33.5 40.0 27.0 41.0 30-31 34.89406291778454 37.0 33.0 40.0 26.5 41.0 32-33 34.53987473350708 37.0 33.0 40.0 25.0 41.0 34-35 34.692918035599334 37.0 33.0 40.0 25.5 41.0 36-37 34.460336752207084 37.0 33.0 40.0 25.0 41.0 38-39 34.23301401838199 37.0 33.0 40.0 24.0 41.0 40-41 34.020352143764256 37.0 33.0 40.0 24.0 41.0 42-43 33.783168387261895 37.0 33.0 40.0 23.5 41.0 44-45 33.288620813262824 37.0 32.0 40.0 21.5 41.0 46-47 32.73647729309444 36.0 31.0 39.5 18.5 41.0 48-49 32.20446379566819 36.0 30.5 39.0 14.5 40.5 50-51 31.91294616860415 36.0 30.0 39.0 13.0 40.0 52-53 31.777389230652282 36.0 30.0 39.0 10.5 40.0 54-55 31.42836562166339 35.0 30.0 39.0 8.5 40.0 56-57 30.92467970288088 35.0 28.5 38.5 7.5 40.0 58-59 30.4563132931586 35.0 28.0 38.0 7.0 40.0 60-61 30.077666690145705 34.0 28.0 38.0 4.0 40.0 62-63 29.914805952703915 34.0 28.0 38.0 2.0 40.0 64-65 29.454847015453623 34.0 27.0 37.5 2.0 40.0 66-67 28.617206498626476 33.5 26.0 36.5 2.0 39.0 68-69 25.353568198127768 31.0 15.5 35.0 2.0 38.5 70-71 24.111938359847215 29.0 10.5 35.0 2.0 38.0 72-73 25.05134133898876 30.0 16.0 35.0 2.0 37.0 74-75 25.743295048549307 31.0 20.5 35.0 2.0 37.0 76-77 25.396019465751866 30.5 21.0 34.0 2.0 36.0 78-79 26.44753166706535 31.5 23.5 35.0 2.0 36.0 80-81 26.461758745187087 32.0 24.0 35.0 2.0 36.0 82-83 26.15666645512284 32.0 23.5 35.0 2.0 36.0 84-85 25.812251944517868 32.0 22.5 34.0 2.0 35.0 86-87 25.371655195644337 31.0 20.5 34.0 2.0 35.0 88-89 24.900557255267614 31.0 19.5 34.0 2.0 35.0 90-91 24.32194681230181 31.0 17.5 34.0 2.0 35.0 92-93 23.77205274694313 31.0 9.5 34.0 2.0 35.0 94-95 22.97051962371234 30.0 2.0 34.0 2.0 35.0 96-97 22.316708487254715 30.0 2.0 34.0 2.0 35.0 98-99 21.50136445770739 29.0 2.0 34.0 2.0 35.0 100-101 20.172234279445682 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3590948940095089 1101 2 0.2852596506771654 1101 3 0.4250096681198059 1101 4 0.4757427536348686 1101 5 0.654817387978639 1101 6 0.4831044605314787 1101 7 0.7058934727756281 1101 8 0.8068749789014333 1101 9 0.77452966518576 1101 10-11 0.9417638428124562 1101 12-13 0.7698577062727239 1101 14-15 0.7930656088334231 1101 16-17 0.8744060084041934 1101 18-19 0.631257831736491 1101 20-21 0.8936047206289572 1101 22-23 0.8718308906507417 1101 24-25 0.9011299401042407 1101 26-27 0.8774100927106545 1101 28-29 0.7931242857610457 1101 30-31 0.5998892881633893 1101 32-33 1.0551448757639648 1101 34-35 1.1472334033896416 1101 36-37 1.148392744167431 1101 38-39 1.2721230992828865 1101 40-41 1.3458430525151286 1101 42-43 1.0143865677361958 1101 44-45 1.1284597458224113 1101 46-47 1.1635408052070844 1101 48-49 1.1426847064691756 1101 50-51 0.7602912308599308 1101 52-53 0.6992383491524947 1101 54-55 0.755877309181642 1101 56-57 0.865793747841213 1101 58-59 0.8196269322893528 1101 60-61 0.8442157181981678 1101 62-63 0.5092630427947107 1101 64-65 0.3849217770035729 1101 66-67 0.1590611836171938 1101 68-69 -6.33400003217513 1101 70-71 -7.0388965031958435 1101 72-73 -4.357398769643915 1101 74-75 -2.4114949379328685 1101 76-77 0.09524865025860052 1101 78-79 0.23363127188193644 1101 80-81 0.36843985978671157 1101 82-83 0.05235336954661918 1101 84-85 0.06757322043687708 1101 86-87 0.03313838468985253 1101 88-89 0.13386237565418568 1101 90-91 0.10355774185008215 1101 92-93 0.25050492291039106 1101 94-95 0.3507497946343854 1101 96-97 -0.03397531033202483 1101 98-99 -0.07761426747424949 1101 100-101 -0.09419896104469316 1102 1 0.35670949163490917 1102 2 0.35306860241814064 1102 3 0.4168211910825441 1102 4 0.3346863920217018 1102 5 0.5919704621011306 1102 6 0.4843198489696263 1102 7 0.5609635331669267 1102 8 0.6730331630570916 1102 9 0.6444668971250849 1102 10-11 0.6763764850129022 1102 12-13 0.655671962509011 1102 14-15 0.9046242229664969 1102 16-17 0.7812275785780258 1102 18-19 0.6681062484904885 1102 20-21 0.7057880444192364 1102 22-23 0.6872282416441138 1102 24-25 0.6837020801602094 1102 26-27 0.7888315029933821 1102 28-29 0.737685827629825 1102 30-31 0.685072352670808 1102 32-33 0.828793903993386 1102 34-35 0.836773230318336 1102 36-37 0.8336244242123811 1102 38-39 1.0938839540520675 1102 40-41 0.9324103094104359 1102 42-43 0.784973087187808 1102 44-45 0.7443972694549643 1102 46-47 0.9056132315545398 1102 48-49 0.9917891002334258 1102 50-51 0.9496222810635793 1102 52-53 0.7446849638905633 1102 54-55 0.5513174418480418 1102 56-57 0.6508979471433065 1102 58-59 0.61901329617125 1102 60-61 0.8052468537490931 1102 62-63 0.6095639189547342 1102 64-65 0.33516836581335596 1102 66-67 0.39233392758616503 1102 68-69 -6.046726229467783 1102 70-71 -7.02249335074832 1102 72-73 -1.0719499401980244 1102 74-75 0.3488334330144873 1102 76-77 0.7820492617340271 1102 78-79 0.5497697397362593 1102 80-81 0.33058510140593356 1102 82-83 0.37273192253215015 1102 84-85 0.03551046317965856 1102 86-87 0.050346934542385924 1102 88-89 0.029798386803346233 1102 90-91 0.09006368915084195 1102 92-93 0.05188047253605177 1102 94-95 0.35129077753251536 1102 96-97 0.18029875165818865 1102 98-99 0.15097436945411502 1102 100-101 0.046143292339490216 1103 1 0.3419985534376444 1103 2 0.2362727520687713 1103 3 0.3347270159402065 1103 4 0.28904224188521255 1103 5 0.2732182935632892 1103 6 0.31247057271335166 1103 7 0.39103783248825863 1103 8 0.37417445428835805 1103 9 0.5314569375622114 1103 10-11 0.48442097482757873 1103 12-13 0.42879751405036615 1103 14-15 0.5708108231058091 1103 16-17 0.5830039708387318 1103 18-19 0.5651874559746091 1103 20-21 0.5337765866178685 1103 22-23 0.5499022461763232 1103 24-25 0.6207462779319144 1103 26-27 0.6744566518534327 1103 28-29 0.5595182836900392 1103 30-31 0.5612679464659749 1103 32-33 0.6976805020730907 1103 34-35 0.7927413369029281 1103 36-37 0.7822636254616668 1103 38-39 0.8532611473449023 1103 40-41 0.7176582845176043 1103 42-43 0.4841609648170504 1103 44-45 0.6567756134921368 1103 46-47 0.834273419761189 1103 48-49 0.9672582305527087 1103 50-51 0.6649614020934713 1103 52-53 0.532447782754744 1103 54-55 0.2654608238534415 1103 56-57 0.611567364541358 1103 58-59 0.7619651587241059 1103 60-61 0.5954704910623612 1103 62-63 0.37389475240733105 1103 64-65 0.48150391314570484 1103 66-67 0.3178841486780044 1103 68-69 -6.3631169692237 1103 70-71 -7.386056528408144 1103 72-73 -0.7389307598934458 1103 74-75 0.7232055802485 1103 76-77 0.825528333132457 1103 78-79 0.5359984088494691 1103 80-81 0.41588625685042047 1103 82-83 0.3145544290635094 1103 84-85 0.10976024810823404 1103 86-87 0.2711791038406979 1103 88-89 0.3359776040120188 1103 90-91 0.09801628841244181 1103 92-93 0.1617484617225422 1103 94-95 0.3193509943625372 1103 96-97 0.36396900821766565 1103 98-99 0.4459824943745687 1103 100-101 0.6695525691661182 1104 1 0.3339723975086528 1104 2 0.2582814984373947 1104 3 0.29990479929292135 1104 4 0.2530847537319829 1104 5 0.44314153451421845 1104 6 0.31488177339841883 1104 7 0.4236662847801824 1104 8 0.4882949883169374 1104 9 0.5586322476927705 1104 10-11 0.4936057764099573 1104 12-13 0.3759634786498367 1104 14-15 0.6341592001711618 1104 16-17 0.5099689961999374 1104 18-19 0.4568380144309785 1104 20-21 0.5271045316856231 1104 22-23 0.5756247988643892 1104 24-25 0.5511195429432547 1104 26-27 0.7603291932981691 1104 28-29 0.6198730671293404 1104 30-31 0.6845000121224629 1104 32-33 0.6118580112310568 1104 34-35 0.2545151632859515 1104 36-37 0.72429687495422 1104 38-39 0.7126752993643706 1104 40-41 0.7704380382335572 1104 42-43 0.7589527052911649 1104 44-45 0.9017164005332248 1104 46-47 0.7675494649338646 1104 48-49 0.9873812043675798 1104 50-51 0.6268768665223057 1104 52-53 0.5134761791963882 1104 54-55 0.6439112324263192 1104 56-57 0.7095602012165401 1104 58-59 0.8345806886727871 1104 60-61 0.9298890108998918 1104 62-63 0.7375696038782955 1104 64-65 0.7256980174323786 1104 66-67 0.6419590148855185 1104 68-69 -6.058518701676295 1104 70-71 -4.108448074169928 1104 72-73 0.6378328917696372 1104 74-75 1.2681215071036185 1104 76-77 1.495125625983075 1104 78-79 0.9394353682578114 1104 80-81 0.621304198474494 1104 82-83 0.5362222534298589 1104 84-85 0.6188935543282454 1104 86-87 0.5680236042546731 1104 88-89 0.6684221693576227 1104 90-91 0.5974582499915364 1104 92-93 0.5253094351727086 1104 94-95 0.8387036016841876 1104 96-97 0.5276281324477026 1104 98-99 0.6354628277384116 1104 100-101 0.5506487970740643 1105 1 0.11900926610709206 1105 2 0.20869645105925017 1105 3 0.2188824543954624 1105 4 0.19120353665257284 1105 5 0.28701441744095035 1105 6 0.21102435921081764 1105 7 0.31256097809539085 1105 8 0.3742545006985978 1105 9 0.44937945388782197 1105 10-11 0.36722385824935344 1105 12-13 0.2806595936686165 1105 14-15 0.3554769639120323 1105 16-17 0.3294900585016336 1105 18-19 0.47233513138724703 1105 20-21 0.5643738579107804 1105 22-23 0.44099514441295184 1105 24-25 0.5268378201740092 1105 26-27 0.5495505344107627 1105 28-29 0.46267051757897804 1105 30-31 0.5121516046369479 1105 32-33 0.6612702771447587 1105 34-35 0.5435759874930497 1105 36-37 0.6877267825379434 1105 38-39 0.6226235193201504 1105 40-41 0.7666004628680909 1105 42-43 0.6046090808810121 1105 44-45 0.6866301103806478 1105 46-47 0.6118120426752611 1105 48-49 0.7925804434107064 1105 50-51 0.5225454450917333 1105 52-53 0.3696631411594211 1105 54-55 0.39175456874761494 1105 56-57 0.3670967162467349 1105 58-59 0.4939758745203662 1105 60-61 0.5730824064708138 1105 62-63 0.27609289145336646 1105 64-65 0.26100747739972974 1105 66-67 0.41193993143705043 1105 68-69 -3.3462415600020634 1105 70-71 -3.28589366314689 1105 72-73 0.3941713595228755 1105 74-75 1.0537977235682625 1105 76-77 1.0081718324780162 1105 78-79 0.6177010918842392 1105 80-81 0.4706675498375823 1105 82-83 0.47350299236594395 1105 84-85 0.42747575899715073 1105 86-87 0.4544126327804996 1105 88-89 0.4846477600629093 1105 90-91 0.43270278477064394 1105 92-93 0.30021931888826714 1105 94-95 0.46684479128687784 1105 96-97 0.3681496091786016 1105 98-99 0.3083174690067345 1105 100-101 0.41812710727852576 1106 1 0.0929545341605369 1106 2 0.16860321183529692 1106 3 0.04591917743991303 1106 4 0.13541870205254014 1106 5 0.21021512251545005 1106 6 0.12907768067688608 1106 7 0.2103800785867378 1106 8 0.33805748591175444 1106 9 0.31889882147544313 1106 10-11 0.3519398892467862 1106 12-13 0.1806672386054302 1106 14-15 0.3288127088189299 1106 16-17 0.26570383413911003 1106 18-19 0.20924478273668967 1106 20-21 0.2084523260394775 1106 22-23 0.3326915621074349 1106 24-25 0.41184678277473097 1106 26-27 0.5949425021304791 1106 28-29 0.48396612097374003 1106 30-31 0.3875596453701675 1106 32-33 0.5697996440611135 1106 34-35 0.4173298006383632 1106 36-37 0.41707706235506947 1106 38-39 0.5920311340915774 1106 40-41 0.42170048516064895 1106 42-43 0.37964179458082015 1106 44-45 0.508104882917884 1106 46-47 0.6888359205684225 1106 48-49 0.7176663816164179 1106 50-51 0.5543164765408086 1106 52-53 0.5040850596283519 1106 54-55 0.32659525986647964 1106 56-57 0.45849576868892683 1106 58-59 0.5085372001731301 1106 60-61 0.9018863737165077 1106 62-63 0.5597968074013231 1106 64-65 0.5710758591841447 1106 66-67 0.7409310856857694 1106 68-69 -2.9586032778048654 1106 70-71 -2.7084785260891238 1106 72-73 0.9467643812079771 1106 74-75 1.2825224237997226 1106 76-77 1.2838147944538782 1106 78-79 1.0633207295515241 1106 80-81 0.903988369507406 1106 82-83 0.7765347174034609 1106 84-85 0.6348693244879513 1106 86-87 0.6956973012222818 1106 88-89 0.683593256014202 1106 90-91 0.7789287135786687 1106 92-93 0.7853899024973607 1106 94-95 0.964151867473543 1106 96-97 1.0279616266049132 1106 98-99 1.0929057321848248 1106 100-101 0.9103992985204528 1107 1 0.047654047993543 1107 2 0.07743936481664804 1107 3 0.04803504372439704 1107 4 -0.0650068824479817 1107 5 0.330170725492124 1107 6 0.2063007719514971 1107 7 0.0875113919007191 1107 8 0.09348127704983256 1107 9 0.14701331937916962 1107 10-11 0.07620702188627604 1107 12-13 0.16119154846229122 1107 14-15 0.2118809560557935 1107 16-17 -0.004908154564219558 1107 18-19 0.15435621233289964 1107 20-21 0.22720125228254773 1107 22-23 0.19736541126606255 1107 24-25 0.1545361934969307 1107 26-27 0.48344923402128614 1107 28-29 0.3679318029487959 1107 30-31 0.3073375698319367 1107 32-33 0.3205233939066474 1107 34-35 0.3163525688063302 1107 36-37 0.46423759692778077 1107 38-39 0.69873469278577 1107 40-41 0.5254743828455517 1107 42-43 0.2445729686454854 1107 44-45 0.44109642246679215 1107 46-47 0.7371625174898639 1107 48-49 0.5301933197288307 1107 50-51 0.47376812959901926 1107 52-53 0.19374331105474596 1107 54-55 -0.007664895236764835 1107 56-57 0.6798290959941546 1107 58-59 0.7855412450343167 1107 60-61 0.620764000088279 1107 62-63 0.3359863040240789 1107 64-65 0.20078230534258168 1107 66-67 0.3901053100941887 1107 68-69 -2.6928849833980415 1107 70-71 -2.366427416766353 1107 72-73 1.3180228092579753 1107 74-75 1.4397068748951831 1107 76-77 0.9306870347101963 1107 78-79 0.8866300710107495 1107 80-81 0.8784043210896364 1107 82-83 0.7953088863532756 1107 84-85 0.6367821188480498 1107 86-87 0.6274986659861774 1107 88-89 0.7734158487641949 1107 90-91 0.6972207091540383 1107 92-93 0.7307160834792761 1107 94-95 0.7593747417362273 1107 96-97 0.8006683832109687 1107 98-99 0.9561030713774805 1107 100-101 1.347218687330475 1108 1 -0.07956798836198331 1108 2 -0.011139831700852199 1108 3 0.020649572859962717 1108 4 0.013064821077968247 1108 5 -0.11574014309498892 1108 6 -0.09092853243277688 1108 7 0.11720340437558008 1108 8 0.2654728322926161 1108 9 0.07607274416660914 1108 10-11 0.23687198883405358 1108 12-13 0.163804193294105 1108 14-15 0.07201601064291907 1108 16-17 0.3071752316449903 1108 18-19 0.18526994583082512 1108 20-21 0.09638557948997573 1108 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-0.5122892516745061 2316 2 -0.49722558060017974 2316 3 -1.0634792404417972 2316 4 -0.3827481978624334 2316 5 -0.2021918676550385 2316 6 -0.6015519574077643 2316 7 -0.7426236963964712 2316 8 -1.2002423820796295 2316 9 -5.372793237825867 2316 10-11 -1.5965746829824425 2316 12-13 -0.8144010485579756 2316 14-15 -0.4841601979843162 2316 16-17 -0.34785872768823367 2316 18-19 -0.5161677855422013 2316 20-21 -3.980274502147509 2316 22-23 -1.388478699229914 2316 24-25 -0.5289172460355687 2316 26-27 -0.5914944397691855 2316 28-29 -0.28436454902787744 2316 30-31 -0.17680365292628863 2316 32-33 -1.67368222377808 2316 34-35 -0.737042943387344 2316 36-37 -0.42079013830133505 2316 38-39 -5.182938841392534 2316 40-41 -5.177330789963175 2316 42-43 -3.9332856205316844 2316 44-45 -2.7439298363404916 2316 46-47 -2.9932102460535788 2316 48-49 -3.4051483629849635 2316 50-51 -1.8428466616249004 2316 52-53 -1.4166961236849716 2316 54-55 -0.6697960083756875 2316 56-57 -4.074996868274148 2316 58-59 -1.3498748751412606 2316 60-61 -1.098800137252777 2316 62-63 -0.7164878779450596 2316 64-65 -0.3672345132521926 2316 66-67 -0.5247523814758459 2316 68-69 2.310358486015513 2316 70-71 3.182396419734406 2316 72-73 1.9504194317781938 2316 74-75 0.4594197581520696 2316 76-77 -1.8063327805908678 2316 78-79 -0.41059019937225827 2316 80-81 -0.3013065324455937 2316 82-83 -0.117766993414957 2316 84-85 0.12603111609961104 2316 86-87 0.20384651727638214 2316 88-89 -0.062277752710276246 2316 90-91 0.054557077895982076 2316 92-93 -0.10287712876452915 2316 94-95 0.25226397885043284 2316 96-97 0.5260075517073943 2316 98-99 0.6649480278775037 2316 100-101 0.6494721944056892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 53.0 4 146.0 5 382.0 6 961.0 7 2070.0 8 4041.0 9 6997.0 10 10900.0 11 15992.0 12 20043.0 13 25185.0 14 31129.0 15 39049.0 16 46771.0 17 51763.0 18 45026.0 19 36237.0 20 34092.0 21 34753.0 22 36326.0 23 40620.0 24 47891.0 25 57871.0 26 71976.0 27 88774.0 28 107656.0 29 127149.0 30 148583.0 31 171410.0 32 197111.0 33 226261.0 34 260248.0 35 286952.0 36 282866.0 37 220535.0 38 85313.0 39 4664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44023130419012 20.01339276372235 15.584294194376433 20.962081737711095 2 16.248939333540495 22.140298489764415 38.036118286848854 23.574643889846236 3 19.581468365165065 26.332281186754393 30.712869988995 23.37338045908554 4 12.968990345935843 17.899163388901474 36.021432378444615 33.11041388671806 5 13.042235074478539 38.981995295358196 32.60611073412916 15.36965889603411 6 30.621422788012858 36.63092970723961 16.829694895679832 15.917952609067697 7 27.343899831439085 35.18125005666353 19.5878661248355 17.886983987061885 8 27.617174941401196 33.30863384761731 19.002087309950532 20.072103901030964 9 27.28723630538467 16.536334745097818 20.08674924314964 36.08967970636787 10-11 25.29359208146193 27.51198867026632 25.44302543784671 21.751393810425043 12-13 26.955205477636913 25.236243316319168 26.920021786689897 20.88852941935403 14-15 23.561879708739013 25.619271317515903 27.073989663178054 23.744859310567033 16-17 21.266121740476724 29.286045800440686 27.360067285284934 22.087765173797656 18-19 20.886985766976633 26.487388863075473 31.869297136047546 20.756328233900348 20-21 22.35726544961549 25.180067968335372 31.94651242099361 20.516154161055525 22-23 21.83741732563522 25.566893117639726 31.83080076121002 20.76488879551503 24-25 21.499392542005964 25.757247650372005 32.109664649710844 20.63369515791118 26-27 21.34973413413616 25.874082955868378 32.100698624736744 20.67548428525872 28-29 20.986988342353975 26.047410631484446 32.263485151988064 20.70211587417351 30-31 20.917787633963822 26.064993757406597 32.049532071378096 20.967686537251485 32-33 20.901591612199525 26.166998630301308 31.787331263442436 21.144078494056735 34-35 21.410037528097252 26.056746165417938 31.70625563601442 20.82696067047039 36-37 21.392724363253087 25.837855194010288 31.725636267024054 21.043784175712567 38-39 21.30731276189093 26.074122605927023 31.847403443412535 20.77116118876951 40-41 21.276394150318186 26.105819145650518 31.769631698979744 20.848155005051552 42-43 21.087681943273605 26.37673820358103 31.170916088625113 21.364663764520255 44-45 21.302779175691057 26.482071151420648 30.840617411570253 21.374532261318038 46-47 21.538411874688638 26.764831364310147 30.12908218596036 21.567674575040854 48-49 21.807364594834915 26.890685481450667 29.727210990707537 21.57473893300688 50-51 21.547523246716356 27.343603323043336 29.5316003888935 21.577273041346807 52-53 21.880122482503744 27.438188274979918 29.080732243281847 21.600956999234484 54-55 21.395608791070007 27.20749680646804 29.35790426852637 22.038990133935588 56-57 21.720545548530758 27.650483818197923 28.85411718090008 21.774853452371236 58-59 21.881713904653484 27.14356664936746 29.017548228505742 21.957171217473313 60-61 21.746400970900872 27.13298273024021 29.56162988589125 21.558986412967666 62-63 21.774890959735654 27.211648303825857 29.346755712147647 21.666705024290835 64-65 21.49979756781478 27.340672270138338 29.21572436024629 21.943805801800583 66-67 21.36737556058588 27.56214200554422 29.08112806536845 21.989354368501445 68-69 21.516255933107452 27.097336145805645 29.615322894130923 21.77108502695598 70-71 21.046488011134684 27.177844580413897 29.854143018797252 21.921524389654166 72-73 21.565494005886602 27.316889593926614 29.015109459206244 22.10250694098054 74-75 21.382505489199445 27.756291581110982 28.622108456149466 22.239094473540113 76-77 20.839784035470753 27.6562147096446 29.204854852433147 22.2991464024515 78-79 21.15350109567538 27.670515843656098 28.809121961826516 22.366861098842 80-81 21.067834377135846 27.97319822018886 28.664147824752767 22.29481957792253 82-83 21.161956888257965 27.95796505770887 28.284492026346726 22.595586027686444 84-85 21.000568903289405 27.699644866959794 28.253536641434184 23.046249588316613 86-87 21.185959878692483 27.643890530321123 28.346531238917716 22.823618352068674 88-89 21.075387000223692 27.564779287783942 28.14843317802821 23.211400533964156 90-91 20.864154544359266 27.72213371673249 28.41940431669277 22.994307422215474 92-93 20.91096445726939 27.726450870478697 28.347040721141692 23.01554395111022 94-95 20.671635892674907 27.37817175497334 28.52127635630261 23.428915996049138 96-97 20.653281809645133 27.305607142807307 28.49101707425634 23.550093973291215 98-99 20.507465492140774 27.322635819959117 28.09822675489677 24.071671933003337 100-101 20.368785847568027 27.166896285235957 28.605132145036166 23.859185722159847 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8440.0 1 5766.0 2 3137.0 3 3279.5 4 3047.0 5 3030.5 6 4912.0 7 9626.0 8 14059.0 9 15604.5 10 13920.5 11 11083.0 12 9839.0 13 9327.0 14 9222.0 15 9176.5 16 9177.0 17 9353.0 18 9558.5 19 9999.5 20 10661.0 21 11492.0 22 12560.5 23 13861.5 24 15312.0 25 17297.0 26 20293.5 27 23510.5 28 26576.0 29 30111.5 30 34160.5 31 38334.5 32 42129.5 33 45516.5 34 49562.5 35 53289.0 36 57535.0 37 62827.0 38 65704.0 39 66002.0 40 66660.5 41 70587.5 42 75677.0 43 80556.5 44 85339.5 45 88843.0 46 92224.0 47 99784.5 48 114536.5 49 117530.5 50 107877.0 51 101696.0 52 97344.5 53 96127.5 54 94670.0 55 89141.5 56 82443.5 57 75720.5 58 69229.0 59 62725.0 60 54850.0 61 45147.5 62 35449.5 63 26852.5 64 19660.5 65 14359.0 66 10519.0 67 7805.5 68 5506.5 69 3635.5 70 2573.0 71 2040.0 72 1638.0 73 1401.0 74 1127.5 75 855.0 76 605.0 77 364.0 78 233.5 79 146.0 80 87.5 81 54.5 82 39.0 83 27.5 84 13.5 85 7.5 86 7.0 87 4.0 88 2.0 89 2.0 90 2.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02043373924177577 2 0.017609280404601985 3 6.276575193719519E-4 4 0.0974612648135892 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010705047691510513 12-13 0.0 14-15 0.0 16-17 5.927876571846213E-4 18-19 1.2204451765565733E-4 20-21 0.0 22-23 1.2204451765565733E-4 24-25 0.004114643738105018 26-27 0.0035044211498267315 28-29 0.0010984006589009158 30-31 2.6152396640497996E-4 32-33 7.671369681212745E-4 34-35 0.003068547872485098 36-37 0.0892145424062855 38-39 0.10267430921059514 40-41 0.08197904600241439 42-43 0.08562294660099044 44-45 0.08452454594208954 46-47 0.08311231652350264 48-49 0.07873614881899264 50-51 0.07810849129962069 52-53 0.06151043689845129 54-55 0.05110178303553308 56-57 0.028593286993611147 58-59 0.021689054280519673 60-61 0.012762369560563022 62-63 0.0020224520068651782 64-65 0.006241705331532189 66-67 0.14938248961052458 68-69 0.6979202916794232 70-71 0.8521671270650804 72-73 0.3473212623169071 74-75 0.05214787890115301 76-77 0.18800086198299326 78-79 0.013825900357276608 80-81 0.002998808148110437 82-83 0.0017086232471792025 84-85 2.6152396640497996E-4 86-87 0.0018481026959285252 88-89 3.6613355296697195E-4 90-91 0.004341297842322668 92-93 0.004567951946540317 94-95 0.07327901538667539 96-97 0.036055437501699907 98-99 0.013233112700091986 100-101 0.01288441407821868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2867806.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.66591361635746 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19312909070894 27.731358968318826 2 14.640110533732663 11.907069405973948 3 6.164548759136036 7.520610219685488 4 3.3188276834596713 5.398526395325869 5 1.9640672063306484 3.9935293674681334 6 1.2933662285112508 3.1557551593771542 7 0.8839970833665098 2.51639843205061 8 0.6503902946642484 2.1158972431786878 9 0.45131425494069966 1.6517795854724298 >10 2.195708295527011 15.583606893512256 >50 0.1421991409785772 3.961299840138462 >100 0.0921234860858398 7.0686587576546875 >500 0.006375297493359628 1.7661814581147073 >1k 0.0033186480102329924 2.212352612084388 >5k 2.6199852712365727E-4 0.7730839512610036 >10k+ 2.6199852712365727E-4 2.6438917103833797 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29761 1.037761968557148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25521 0.8899137528828659 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19163 0.6682111690958176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9542 0.3327282249915092 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6383 0.2225743303417316 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5843 0.20374460476057307 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4656 0.16235407834421156 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4207 0.1466975102221001 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2932 0.10223843593325349 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.4869862187330666E-5 0.0 16-17 0.0 0.0 0.0 1.04609586561992E-4 0.0 18-19 0.0 0.0 0.0 1.7434931093665332E-4 0.0 20-21 0.0 3.4869862187330666E-5 0.0 3.835684840606373E-4 0.0 22-23 0.0 3.4869862187330666E-5 0.0 8.020068303086053E-4 0.0 24-25 0.0 3.4869862187330666E-5 0.0 0.0013947944874932265 0.0 26-27 0.0 3.4869862187330666E-5 0.0 0.0023362807665511546 0.0 28-29 0.0 3.4869862187330666E-5 0.0 0.005125869741537607 0.0 30-31 0.0 3.4869862187330666E-5 0.0 0.011402444935257126 0.0 32-33 0.0 3.4869862187330666E-5 0.0 0.021811098798175332 0.0 34-35 0.0 3.4869862187330666E-5 0.0 0.03863580730356238 0.0 36-37 0.0 3.4869862187330666E-5 0.0 0.06644452239795859 0.0 38-39 0.0 3.4869862187330666E-5 0.0 0.10642281939573318 0.0 40-41 0.0 3.4869862187330666E-5 0.0 0.1485107430558413 0.0 42-43 0.0 3.4869862187330666E-5 0.0 0.19108684478657204 0.0 44-45 0.0 3.4869862187330666E-5 0.0 0.2429557647902264 0.0 46-47 0.0 3.4869862187330666E-5 0.0 0.30875519473771934 0.0 48-49 0.0 3.4869862187330666E-5 0.0 0.3811973334318988 0.0 50-51 0.0 3.4869862187330666E-5 0.0 0.4626882013636906 0.0 52-53 0.0 3.4869862187330666E-5 0.0 0.5403259495237822 0.0 54-55 0.0 3.4869862187330666E-5 0.0 0.6251120194322768 0.0 56-57 1.7434931093665333E-5 3.4869862187330666E-5 0.0 0.7245783013216376 0.0 58-59 3.4869862187330666E-5 3.4869862187330666E-5 0.0 0.8302688536114368 0.0 60-61 3.4869862187330666E-5 3.4869862187330666E-5 0.0 0.9432297721672944 0.0 62-63 3.4869862187330666E-5 3.4869862187330666E-5 0.0 1.0509602113950525 0.0 64-65 3.4869862187330666E-5 3.4869862187330666E-5 0.0 1.1618986779440452 0.0 66-67 6.973972437466133E-5 3.4869862187330666E-5 0.0 1.2809618223826855 0.0 68-69 6.973972437466133E-5 3.4869862187330666E-5 0.0 1.4191859560932643 0.0 70-71 6.973972437466133E-5 3.4869862187330666E-5 0.0 1.5964992053158409 0.0 72-73 6.973972437466133E-5 3.4869862187330666E-5 0.0 1.7937057109162893 0.0 74-75 6.973972437466133E-5 3.4869862187330666E-5 0.0 1.9994727676837276 0.0 76-77 6.973972437466133E-5 3.4869862187330666E-5 0.0 2.229038505394019 0.0 78-79 6.973972437466133E-5 3.4869862187330666E-5 0.0 2.4939622833622637 0.0 80-81 6.973972437466133E-5 3.4869862187330666E-5 0.0 2.792605218065657 0.0 82-83 6.973972437466133E-5 3.4869862187330666E-5 0.0 3.1031387757749305 0.0 84-85 6.973972437466133E-5 3.4869862187330666E-5 0.0 3.409976128092347 1.7434931093665333E-5 86-87 6.973972437466133E-5 3.4869862187330666E-5 0.0 3.732905921809216 3.4869862187330666E-5 88-89 6.973972437466133E-5 3.4869862187330666E-5 0.0 4.083051642963296 3.4869862187330666E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20210 0.0 34.439713 1 TATAACG 165 7.6397555E-11 31.64588 2 GTATCAA 29425 0.0 29.640434 1 GTACATG 22635 0.0 23.932993 1 TATCACG 180 1.9655636E-7 23.73441 2 TATCAAC 37075 0.0 23.494347 2 TACATGG 23040 0.0 23.178135 2 ATCAACG 37665 0.0 23.085287 3 TCAACGC 38040 0.0 22.773554 4 CAACGCA 38550 0.0 22.484875 5 AACGCAG 39310 0.0 22.07429 6 ACATGGG 23155 0.0 21.87094 3 GAGTACT 22485 0.0 21.791548 12-13 AGTACTT 22680 0.0 21.311533 12-13 GTACTTT 23430 0.0 20.963219 14-15 GTACACG 560 0.0 20.347689 1 CATGGGG 7520 0.0 19.883883 4 ACGCAGA 44245 0.0 19.515713 7 TACTTTT 24840 0.0 19.477442 14-15 CGCAGAG 44355 0.0 19.445932 8 >>END_MODULE