FastQCFastQC Report
Fri 27 May 2016
SRR939417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130730.4415821564733688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96380.32555410572097665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68630.23181965423978657No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44610.15068446416489698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA213800.030.3388751
GGTATCA148300.026.9290641
TATCAAC258700.025.0009882
GTACATG134350.024.9379921
TACATGG135500.024.4413452
ATCAACG264650.024.34433
TCAACGC267900.023.8863414
CAACGCA268150.023.7716585
AACGCAG274350.023.1452356
TAAGGTG14550.022.866095
GAGTACT133650.022.76695612-13
AGTACTT141450.021.95156112-13
AGAGTAC201800.021.89541810-11
ACATGGG142800.021.8211143
GTACTTT146000.021.08202614-15
ACGCAGA297450.020.9838267
CGCAGAG298400.020.9486228
GTAAGGT13950.019.8847434
GCAGAGT293150.019.7621049
CATGGGA76950.019.7022974
Produced by FastQC (version 0.11.5)