FastQCFastQC Report
Fri 27 May 2016
SRR939417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960491
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177580.5998329331181889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150190.5073144961427006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108530.3665945952884167No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65000.2195581746406255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42860.14477328253995705No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38860.13126201025438008No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38670.1306202248208152No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32150.10859685099532476No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30840.1041719093217983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA239600.027.5136851
GGTATCA171550.026.2999361
TATCAAC292350.022.4289722
GTACATG154300.022.4215351
ATCAACG293650.022.2613813
TCAACGC294450.022.20094
TACATGG153150.022.1499332
CAACGCA295350.022.0690945
AACGCAG301300.021.6804456
GAGTACT155200.021.55026812-13
AGTACTT161700.021.25529312-13
AGAGTAC237450.021.21659510-11
GTACTTT162050.020.52238314-15
ACATGGG158050.020.0809083
TAAGGTA7500.019.5795844
ACGCAGA335000.019.4570357
CGCAGAG336250.019.3001798
TACTTTT175600.019.26253314-15
TAAGGTG15350.019.1331445
GTAAGGT13600.018.8086624
Produced by FastQC (version 0.11.5)