FastQCFastQC Report
Fri 27 May 2016
SRR939416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940079
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128150.4358726415174558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88750.30186263702437927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67490.22955165490451107No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40870.13900987014294514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA204200.031.1426031
GTACATG133650.025.9469381
GGTATCA142200.025.4440731
TATCAAC245550.025.285732
TACATGG135350.025.2806422
ATCAACG256050.024.3349193
CAACGCA260350.024.067685
TCAACGC260750.024.0018834
AACGCAG265350.023.4662596
ACATGGG142500.022.6727453
GAGTACT126450.022.03137412-13
AGAGTAC194700.021.91157510-11
AGTACTT134000.021.79371312-13
ACGCAGA291650.021.0852557
CGCAGAG294200.020.7558178
GTATCGA2701.24437065E-820.4163421
GTACTTT138050.020.177214-15
CATGGGA77050.019.7343564
GCAGAGT289600.019.730399
TACTTTT152900.019.08652314-15
Produced by FastQC (version 0.11.5)