##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2940079 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.145152222100155 31.0 27.0 31.0 10.0 33.0 2 27.164426193990025 31.0 26.0 31.0 16.0 34.0 3 27.437928368591457 31.0 26.0 31.0 16.0 34.0 4 31.271333525391665 35.0 30.0 37.0 19.0 37.0 5 31.677392002051647 35.0 32.0 37.0 19.0 37.0 6 31.505358869608607 35.0 31.0 37.0 17.0 37.0 7 31.471919972218434 35.0 31.0 37.0 17.0 37.0 8 31.392961549672645 35.0 30.0 37.0 17.0 37.0 9 32.47688174365383 35.0 31.0 39.0 17.0 39.0 10-11 31.596992801894096 35.0 30.0 38.5 14.0 39.0 12-13 31.33277660226137 35.0 29.5 38.0 15.5 39.0 14-15 32.125502580032716 36.0 30.0 39.0 17.0 40.0 16-17 32.033019350840576 36.0 29.5 39.0 16.5 40.0 18-19 31.998249706895628 36.0 29.5 39.0 16.5 40.0 20-21 31.872991678114772 36.0 29.0 39.0 16.0 40.0 22-23 32.04347485220635 36.0 29.5 39.0 16.0 40.0 24-25 32.095863580536445 36.0 30.0 39.0 15.5 41.0 26-27 32.17719574882172 36.0 30.0 39.0 16.0 41.0 28-29 31.767750798532965 36.0 30.0 39.0 10.5 41.0 30-31 31.44907619829263 36.0 29.0 39.0 10.0 40.0 32-33 31.27070395047208 35.0 29.0 39.0 9.5 40.0 34-35 30.81000408492425 35.0 27.0 39.0 9.0 40.0 36-37 30.4249015077486 35.0 26.5 38.5 9.0 40.0 38-39 30.017342051012918 35.0 25.0 38.0 8.0 40.0 40-41 29.84860253755086 35.0 25.5 38.0 8.0 40.0 42-43 29.53976423762763 34.5 25.0 38.0 6.5 40.0 44-45 29.152259514115094 34.0 24.5 38.0 2.0 40.0 46-47 28.529602095726 33.5 23.5 38.0 2.0 40.0 48-49 28.14470682590502 33.0 23.0 38.0 2.0 40.0 50-51 27.4475325322891 33.0 22.0 37.5 2.0 39.5 52-53 27.541165900644167 33.0 22.0 38.0 2.0 39.5 54-55 27.660418648614545 33.0 22.0 38.0 2.0 40.0 56-57 27.18331735303711 33.0 20.5 38.0 2.0 40.0 58-59 26.776479135424594 33.0 19.5 37.5 2.0 40.0 60-61 26.14758327922481 32.0 16.0 37.0 2.0 39.0 62-63 25.578541767074967 31.0 13.5 36.5 2.0 39.0 64-65 25.051055600886915 31.0 12.0 36.0 2.0 39.0 66-67 24.650332525078404 31.0 8.5 35.5 2.0 39.0 68-69 23.954101403397665 30.0 7.5 35.0 2.0 38.0 70-71 23.3242747898951 29.5 4.5 34.5 2.0 37.5 72-73 22.845206370305014 29.0 2.0 34.0 2.0 37.0 74-75 22.122785646236036 29.0 2.0 34.0 2.0 36.0 76-77 21.354665299809973 27.5 2.0 34.0 2.0 36.0 78-79 20.841977715564788 27.0 2.0 33.5 2.0 35.0 80-81 20.294482393160184 26.0 2.0 33.0 2.0 35.0 82-83 19.559010149047015 26.0 2.0 33.0 2.0 35.0 84-85 18.86333972658558 25.0 2.0 32.0 2.0 35.0 86-87 18.10623694125226 23.5 2.0 32.0 2.0 34.0 88-89 17.32123864698874 20.0 2.0 31.5 2.0 34.0 90-91 16.52590474609696 18.0 2.0 31.0 2.0 34.0 92-93 15.697202354086404 9.0 2.0 31.0 2.0 34.0 94-95 14.65664136916049 2.0 2.0 30.5 2.0 34.0 96-97 13.574537112778263 2.0 2.0 29.5 2.0 34.0 98-99 12.557831269159774 2.0 2.0 29.0 2.0 34.0 100-101 11.216191639748455 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.039362823637152 1101 2 3.901612502017187 1101 3 3.4888748798415534 1101 4 3.216750559000225 1101 5 2.5386859815824145 1101 6 2.9168352837768694 1101 7 2.699128880375433 1101 8 2.6447907024625295 1101 9 2.751734460074019 1101 10-11 3.6043686337587957 1101 12-13 3.5838297265321124 1101 14-15 3.1666964998812723 1101 16-17 3.0576669359741864 1101 18-19 2.9882891000871084 1101 20-21 2.95264378025502 1101 22-23 2.967996062376603 1101 24-25 3.0784057458843925 1101 26-27 2.7118443129878393 1101 28-29 3.0458427042889618 1101 30-31 3.052744594633065 1101 32-33 2.997677163108861 1101 34-35 3.1297643122864365 1101 36-37 3.165932519476474 1101 38-39 3.1458281972910065 1101 40-41 2.919601503239999 1101 42-43 2.7483599060550326 1101 44-45 2.82893281084392 1101 46-47 2.9082004098874243 1101 48-49 2.7668583484914 1101 50-51 2.768681316591092 1101 52-53 2.7180333707128277 1101 54-55 2.5763278309177124 1101 56-57 2.922633078267797 1101 58-59 2.719406205367669 1101 60-61 2.752174350746156 1101 62-63 2.827662201639317 1101 64-65 2.657269712850823 1101 66-67 2.49703037374762 1101 68-69 2.59157654086221 1101 70-71 2.52232923333656 1101 72-73 2.463175522652307 1101 74-75 2.410165359360043 1101 76-77 2.392062278853743 1101 78-79 2.0590108281996926 1101 80-81 2.30136759534998 1101 82-83 1.9414703388139216 1101 84-85 1.9878854436809092 1101 86-87 2.10502205965669 1101 88-89 1.8715773591930258 1101 90-91 1.7464552198799836 1101 92-93 1.547733651316502 1101 94-95 1.6693800481733465 1101 96-97 1.749434091537621 1101 98-99 1.5009664133751048 1101 100-101 1.2641951589822735 1102 1 3.703305618240048 1102 2 2.7026300574475073 1102 3 2.917418584988191 1102 4 3.05945860306678 1102 5 1.80699231441481 1102 6 2.5149654633536827 1102 7 2.4754262929011617 1102 8 2.602301071825572 1102 9 2.549329308947442 1102 10-11 2.189009750682473 1102 12-13 3.2861131729177053 1102 14-15 3.3576581298348778 1102 16-17 2.962652542399855 1102 18-19 2.9236117295288864 1102 20-21 3.1744830643163517 1102 22-23 3.05284260124861 1102 24-25 2.964402562588628 1102 26-27 2.800178983754609 1102 28-29 3.1005286303374042 1102 30-31 3.249625260858682 1102 32-33 2.8429366677344063 1102 34-35 3.1038584308463264 1102 36-37 3.08888125221576 1102 38-39 3.2147323509576857 1102 40-41 2.8384869736762575 1102 42-43 2.960096377898825 1102 44-45 2.7217346709313723 1102 46-47 2.7574536251726904 1102 48-49 2.4503304788288816 1102 50-51 2.6165570264857863 1102 52-53 3.0063552626944876 1102 54-55 2.875980812883803 1102 56-57 2.8846848508945833 1102 58-59 2.799963088147244 1102 60-61 2.9256536753184506 1102 62-63 2.8265106646458413 1102 64-65 2.7100088309751236 1102 66-67 2.4882170532509242 1102 68-69 2.518640901698351 1102 70-71 2.5586288102583836 1102 72-73 2.351929817413108 1102 74-75 2.3945112199865477 1102 76-77 2.2773021950140233 1102 78-79 1.9714659408027408 1102 80-81 2.000189156546579 1102 82-83 1.8790928943395322 1102 84-85 1.8825937419619159 1102 86-87 1.654662960481069 1102 88-89 1.5443725678462528 1102 90-91 1.465166977110968 1102 92-93 1.1491467535358915 1102 94-95 1.1227842756881277 1102 96-97 0.8814198841149672 1102 98-99 0.7341514634556923 1102 100-101 0.32598951821213085 1103 1 3.779834491548062 1103 2 3.6548484554576994 1103 3 3.140355691408807 1103 4 2.9641533051637126 1103 5 2.347715100632225 1103 6 2.50650959293462 1103 7 2.3403656735614007 1103 8 2.243990917071784 1103 9 2.665726607045279 1103 10-11 3.3195915025976177 1103 12-13 2.5161804820925617 1103 14-15 3.0097538639618655 1103 16-17 2.482105177961582 1103 18-19 2.45717312081171 1103 20-21 2.6946451317847853 1103 22-23 2.796419040856488 1103 24-25 2.851763034640438 1103 26-27 2.63074990716488 1103 28-29 2.1639840493574276 1103 30-31 2.830094239094148 1103 32-33 2.70775216725562 1103 34-35 2.399056660956372 1103 36-37 2.854154642800683 1103 38-39 2.912393520699947 1103 40-41 2.7902609465152217 1103 42-43 2.807432210664107 1103 44-45 2.6987573189902534 1103 46-47 2.588892096948584 1103 48-49 2.4421817119090257 1103 50-51 2.703242631624228 1103 52-53 2.5785288961219557 1103 54-55 2.4702922721300737 1103 56-57 2.5940442294449113 1103 58-59 2.5684455961811317 1103 60-61 2.734037575487246 1103 62-63 2.885479354797429 1103 64-65 2.69311737773398 1103 66-67 2.3887967966939705 1103 68-69 2.5777505906489147 1103 70-71 2.528370873750447 1103 72-73 2.3698696322279247 1103 74-75 2.36954593713687 1103 76-77 2.4555316507789477 1103 78-79 2.340628691963193 1103 80-81 2.1203424496293195 1103 82-83 2.31175062533411 1103 84-85 2.2068000658087676 1103 86-87 2.1671966378920473 1103 88-89 2.2611105454162868 1103 90-91 2.0861417910737075 1103 92-93 1.9843954896185991 1103 94-95 2.091257349989421 1103 96-97 2.1657814943009654 1103 98-99 2.077640387442125 1103 100-101 1.6248800909715673 1104 1 3.861023820313086 1104 2 3.505098100086922 1104 3 2.8087971106913123 1104 4 2.7653805027890854 1104 5 1.7102569701996266 1104 6 2.132922129697448 1104 7 2.318004758463495 1104 8 2.2243856059564777 1104 9 2.4396397302558483 1104 10-11 3.4146420109669435 1104 12-13 3.330645734713368 1104 14-15 3.099781679796134 1104 16-17 3.0169821996781074 1104 18-19 2.751988871792115 1104 20-21 2.516510323430307 1104 22-23 2.3137289916712866 1104 24-25 2.5019226936067476 1104 26-27 2.4824653527849314 1104 28-29 2.4420133862626834 1104 30-31 2.051551935517214 1104 32-33 2.20603981624582 1104 34-35 2.7407720581833495 1104 36-37 2.84100547825631 1104 38-39 3.036532856099921 1104 40-41 3.05259258634538 1104 42-43 2.943244848950524 1104 44-45 2.897892305380335 1104 46-47 2.965560926021926 1104 48-49 2.6969695069699284 1104 50-51 2.501768094408547 1104 52-53 2.708034260240421 1104 54-55 2.5974053624333493 1104 56-57 2.840173101241703 1104 58-59 2.7677731360984197 1104 60-61 2.7759876282229676 1104 62-63 2.7529524794445095 1104 64-65 2.901599615307841 1104 66-67 2.7933456445438303 1104 68-69 2.709578058613328 1104 70-71 2.7849408077397335 1104 72-73 2.615583087618795 1104 74-75 2.6830430734125557 1104 76-77 2.737023130667417 1104 78-79 2.5198192336116776 1104 80-81 2.596234798963188 1104 82-83 2.50816311879786 1104 84-85 2.2849554030181345 1104 86-87 2.3416200788897967 1104 88-89 2.249148412239034 1104 90-91 2.192650635164185 1104 92-93 2.050253253180699 1104 94-95 2.2617216001093237 1104 96-97 2.219610393172413 1104 98-99 2.0317740608514754 1104 100-101 1.6132874007721103 1105 1 3.4942709082511385 1105 2 3.0529848575279743 1105 3 2.8833938315574237 1105 4 2.68881587155812 1105 5 -0.0798410379481247 1105 6 1.6834807196552006 1105 7 1.9526356293662204 1105 8 1.9950324105850612 1105 9 2.3709755799615593 1105 10-11 2.493521991472143 1105 12-13 3.171057922515363 1105 14-15 2.9011080568427765 1105 16-17 2.7549292606367395 1105 18-19 2.794766306283421 1105 20-21 2.79614659789544 1105 22-23 2.5524120565671105 1105 24-25 2.0808214027073753 1105 26-27 2.39021362442141 1105 28-29 2.4483942059284125 1105 30-31 2.4815979438690583 1105 32-33 2.3356827374425464 1105 34-35 2.885341883250586 1105 36-37 2.880239112663549 1105 38-39 3.0087874871824276 1105 40-41 3.0518725806389106 1105 42-43 2.888227018815723 1105 44-45 2.8491651075519293 1105 46-47 2.8851667023132457 1105 48-49 2.664741220493916 1105 50-51 2.734790814626539 1105 52-53 2.845440632194933 1105 54-55 2.810089126643831 1105 56-57 3.05094543950727 1105 58-59 2.8842597949391795 1105 60-61 3.021219472876137 1105 62-63 2.947411315186283 1105 64-65 3.0321500414751092 1105 66-67 2.8145994007150925 1105 68-69 2.9236758957719893 1105 70-71 2.8336235091557533 1105 72-73 2.694786445694355 1105 74-75 2.687120446487711 1105 76-77 2.881529773388788 1105 78-79 2.7484611263141474 1105 80-81 2.826567171378791 1105 82-83 2.959324487959723 1105 84-85 2.7174699170396615 1105 86-87 2.6206244445723215 1105 88-89 2.587858260306181 1105 90-91 2.4349959723753187 1105 92-93 2.273738126874882 1105 94-95 2.505817485057216 1105 96-97 2.7635039922248392 1105 98-99 2.6262236474827905 1105 100-101 2.2905540258348456 1106 1 3.3413730532820054 1106 2 2.790437418095234 1106 3 2.3815602815642016 1106 4 1.561011203494779 1106 5 1.9602276841111639 1106 6 2.387189370609434 1106 7 2.306882920138044 1106 8 0.8239827668850523 1106 9 1.9046573323188554 1106 10-11 0.9212046069069828 1106 12-13 1.7130186562274439 1106 14-15 1.994133603118673 1106 16-17 2.0151404154241597 1106 18-19 1.9595307204886794 1106 20-21 2.117676108076676 1106 22-23 2.400773064127325 1106 24-25 2.675118901937534 1106 26-27 2.5019146209500427 1106 28-29 2.1089665673929723 1106 30-31 2.2093021451474293 1106 32-33 2.474280950626266 1106 34-35 2.7334822681731943 1106 36-37 1.8290481600528459 1106 38-39 2.8246943481857976 1106 40-41 2.2251162062698597 1106 42-43 2.327960260890098 1106 44-45 2.5859079355574615 1106 46-47 2.7897346550651854 1106 48-49 2.8082497678289826 1106 50-51 2.6354286121865904 1106 52-53 2.673646971486992 1106 54-55 2.7189204170694232 1106 56-57 2.8515491930574903 1106 58-59 2.9059638084990773 1106 60-61 2.9236187340418702 1106 62-63 3.0564453380020886 1106 64-65 2.9464653739603257 1106 66-67 2.566725793309228 1106 68-69 2.8939992346104226 1106 70-71 2.8648889204595775 1106 72-73 2.902171725039569 1106 74-75 3.18537683750921 1106 76-77 3.49927284699562 1106 78-79 3.21243258135139 1106 80-81 3.0615559714961975 1106 82-83 3.20711376453297 1106 84-85 3.1239891445585535 1106 86-87 3.141009976286682 1106 88-89 3.187750491379429 1106 90-91 2.875350608905869 1106 92-93 3.1028923197914082 1106 94-95 3.112193839938458 1106 96-97 2.9927221412173832 1106 98-99 2.8623786975995262 1106 100-101 2.3748749738451984 1107 1 3.0902153842481717 1107 2 3.3067511137970484 1107 3 -0.028812689240009348 1107 4 1.064375395253041 1107 5 1.569391752787606 1107 6 2.1037516013201483 1107 7 2.140385309294601 1107 8 1.9861257559832453 1107 9 2.3601220235567553 1107 10-11 1.7333723519738626 1107 12-13 2.3711736777399857 1107 14-15 2.6037759620823273 1107 16-17 1.2031281959774596 1107 18-19 0.4230777239359611 1107 20-21 -1.1065155316156456 1107 22-23 0.5497564247505444 1107 24-25 1.3409120816442588 1107 26-27 0.9893395641805576 1107 28-29 1.9368764407502717 1107 30-31 2.7003825380760027 1107 32-33 1.4792938191402243 1107 34-35 1.0202207854771324 1107 36-37 0.8902660179319746 1107 38-39 2.0234253490077485 1107 40-41 2.387255896484625 1107 42-43 1.5017513683243067 1107 44-45 0.9539223825632419 1107 46-47 1.8999212819846072 1107 48-49 1.6225459142359142 1107 50-51 1.7193250983880795 1107 52-53 1.4235597662207127 1107 54-55 2.0958276497900634 1107 56-57 2.5099088887723084 1107 58-59 2.573535229803401 1107 60-61 2.485169301048213 1107 62-63 2.339850405316522 1107 64-65 1.636436835271983 1107 66-67 2.2335635238151177 1107 68-69 2.423331758617689 1107 70-71 0.9182030361235931 1107 72-73 1.933131506965207 1107 74-75 2.3802620095923572 1107 76-77 2.4189589003874303 1107 78-79 2.494426539326863 1107 80-81 2.1688320270337123 1107 82-83 2.164057216125059 1107 84-85 2.3927139415672585 1107 86-87 2.4045520361898127 1107 88-89 2.4258346375767665 1107 90-91 2.5102392749368576 1107 92-93 2.471307748468405 1107 94-95 2.4365015238316072 1107 96-97 2.4099223186112564 1107 98-99 2.2533065195600326 1107 100-101 2.0502777053589245 1108 1 2.1865595864940133 1108 2 2.2097944092993593 1108 3 1.7923276458347495 1108 4 1.6971652829609667 1108 5 1.846969166266664 1108 6 -0.1563572879690689 1108 7 0.4577675627424327 1108 8 -0.3689704228834252 1108 9 1.3368805655612022 1108 10-11 2.698810511264483 1108 12-13 1.8011371477854219 1108 14-15 2.058642752850762 1108 16-17 0.04498471832578943 1108 18-19 0.28056196101037045 1108 20-21 1.1267631569089467 1108 22-23 -0.8857652029841923 1108 24-25 1.0134749832235563 1108 26-27 1.2982891028022259 1108 28-29 1.7113859385384593 1108 30-31 0.5924079989539202 1108 32-33 0.6553283674698562 1108 34-35 1.0966659705288713 1108 36-37 0.06793300486125631 1108 38-39 1.384172661804243 1108 40-41 -0.02003898531845394 1108 42-43 1.0647260075284528 1108 44-45 1.8986336457990944 1108 46-47 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0.35454266096191134 2312 50-51 -0.6839248641901143 2312 52-53 -0.9224987754811771 2312 54-55 -0.9897900738227179 2312 56-57 -0.3807217144025685 2312 58-59 -0.6000911745911566 2312 60-61 -0.20191441502851504 2312 62-63 -0.6537676962834063 2312 64-65 -1.9966244675668001 2312 66-67 0.8347868129925189 2312 68-69 0.2635153462218689 2312 70-71 -2.7314396260258924 2312 72-73 -3.1374602852215503 2312 74-75 -0.8638106727056751 2312 76-77 -0.3027060630038143 2312 78-79 -0.26520015259638186 2312 80-81 -1.5652602061380527 2312 82-83 0.19120606229506265 2312 84-85 1.4210507281165157 2312 86-87 1.7893017623914318 2312 88-89 2.0189931240343775 2312 90-91 2.3549247863986977 2312 92-93 2.490325698011965 2312 94-95 2.086727723833116 2312 96-97 1.6012714473562628 2312 98-99 0.8818304958986811 2312 100-101 1.744236130003257 2313 1 -7.841253165725519 2313 2 -4.4418924349298585 2313 3 -2.0285763089675086 2313 4 0.24494575108041516 2313 5 0.5746743048192577 2313 6 -2.542518170401138 2313 7 -1.0006128515701853 2313 8 -2.218580323340781 2313 9 0.46684021043609647 2313 10-11 -1.3692147779472492 2313 12-13 -2.3644426208451854 2313 14-15 -3.629874868887203 2313 16-17 -3.6887467855752085 2313 18-19 -1.1452088218790593 2313 20-21 -2.6545282070942804 2313 22-23 -1.4602742156288002 2313 24-25 -2.747194911853125 2313 26-27 -2.107153458403044 2313 28-29 -2.921546533885536 2313 30-31 -4.535275616865288 2313 32-33 -6.135936093116641 2313 34-35 -4.975636961943554 2313 36-37 -2.338367775337826 2313 38-39 -1.9146166844427306 2313 40-41 -3.617978183596634 2313 42-43 -2.843049079907651 2313 44-45 -3.2658242503537167 2313 46-47 -1.7969697449286492 2313 48-49 -2.472891793837249 2313 50-51 -3.156067766133713 2313 52-53 -0.6471441431883918 2313 54-55 -2.0100537916359755 2313 56-57 -2.446733015216079 2313 58-59 -1.4010937116913347 2313 60-61 -1.8386548788470947 2313 62-63 -3.7012594064240183 2313 64-65 -5.3052028092365155 2313 66-67 -4.0281768660164055 2313 68-69 -2.6860555661072283 2313 70-71 -1.457303056838736 2313 72-73 -1.5457881563112217 2313 74-75 -2.5518791682073356 2313 76-77 -2.462502881324422 2313 78-79 -2.6851862092286503 2313 80-81 -3.2357482033314575 2313 82-83 -1.2033454099605336 2313 84-85 -0.9437072203957193 2313 86-87 -2.321314530326932 2313 88-89 -3.4127234885612445 2313 90-91 -3.332578530578921 2313 92-93 -1.458953837218301 2313 94-95 -0.3809149441127868 2313 96-97 -0.6961334182794356 2313 98-99 -0.44188480851543055 2313 100-101 -0.030386871797732695 2314 1 -7.79400163799578 2314 2 -3.809729400224448 2314 3 -24.964815211308107 2314 4 -13.390589819079853 2314 5 -6.833467341669849 2314 6 -5.1601101224361905 2314 7 -4.232481870364143 2314 8 -3.480506704062563 2314 9 -3.8866058289966254 2314 10-11 -14.525154908236651 2314 12-13 -13.08679936015108 2314 14-15 -5.022984231276226 2314 16-17 -3.220468796413442 2314 18-19 -3.2054244335474564 2314 20-21 -4.224580380506019 2314 22-23 -4.399597402530681 2314 24-25 -5.206746105211824 2314 26-27 -4.037955228263531 2314 28-29 -3.3061849231395435 2314 30-31 -3.8440715928828126 2314 32-33 -4.144677301464384 2314 34-35 -3.286270537407635 2314 36-37 -4.159385589243051 2314 38-39 -1.3463120320733104 2314 40-41 -2.2906909127560944 2314 42-43 -4.502606758911455 2314 44-45 -5.1587246308448975 2314 46-47 -4.163015951546885 2314 48-49 -2.67989556992395 2314 50-51 -2.3968653563562405 2314 52-53 -0.9940108559752012 2314 54-55 -3.434287044259058 2314 56-57 -3.13556977873505 2314 58-59 -1.0806704152541151 2314 60-61 -3.6286677060118606 2314 62-63 -4.484105595747206 2314 64-65 -4.003969816039238 2314 66-67 -5.124650836960715 2314 68-69 -4.333405819768014 2314 70-71 -4.928261779310777 2314 72-73 -4.237060639880923 2314 74-75 -3.652561602197885 2314 76-77 -3.672944049317042 2314 78-79 -4.436213103037016 2314 80-81 -6.675028152599301 2314 82-83 -6.092754351503249 2314 84-85 -4.6637291274636326 2314 86-87 -4.066590818808425 2314 88-89 -3.201316680075138 2314 90-91 -4.848623295942694 2314 92-93 -4.911623040492831 2314 94-95 -5.3300927084388565 2314 96-97 -4.870316226996142 2314 98-99 -5.705991086619893 2314 100-101 -4.784779094788625 2315 1 -24.47724993731551 2315 2 -24.583538924033974 2315 3 -13.055966834251636 2315 4 -28.812358526562846 2315 5 -15.252075347344327 2315 6 -11.867237326363558 2315 7 -9.217854053919648 2315 8 -8.058952566622793 2315 9 -5.346159092276146 2315 10-11 -29.204364091910122 2315 12-13 -28.89632316967489 2315 14-15 -23.470692471975795 2315 16-17 -13.767904466991144 2315 18-19 -8.641394464067446 2315 20-21 -6.544788643891408 2315 22-23 -3.405018557208649 2315 24-25 -5.117533930791144 2315 26-27 -5.009036763367568 2315 28-29 -16.97075645984671 2315 30-31 -16.244412598560316 2315 32-33 -9.026958504296786 2315 34-35 -6.954787232658436 2315 36-37 -4.646682097441708 2315 38-39 -5.008259953749761 2315 40-41 -8.308598909658723 2315 42-43 -7.74063686979974 2315 44-45 -4.602966289269048 2315 46-47 -3.2750690598066434 2315 48-49 -4.083901942370662 2315 50-51 -2.7182400489633842 2315 52-53 -6.4458084046967805 2315 54-55 -6.193658934411296 2315 56-57 -7.017985111475525 2315 58-59 -6.985263216136143 2315 60-61 -4.599988765560621 2315 62-63 -3.3446396048154874 2315 64-65 -4.232943180618509 2315 66-67 -6.591978247026564 2315 68-69 -6.430275309004347 2315 70-71 -7.215917593081372 2315 72-73 -6.394544181318839 2315 74-75 -6.776939916218019 2315 76-77 -6.579167771576067 2315 78-79 -6.2004296444779605 2315 80-81 -7.268163650462297 2315 82-83 -6.511756806865119 2315 84-85 -5.890002784992452 2315 86-87 -6.2046057680073154 2315 88-89 -5.789113353880277 2315 90-91 -5.25560018741071 2315 92-93 -5.157466242157668 2315 94-95 -5.34610313106055 2315 96-97 -5.4273323082174265 2315 98-99 -5.348060937014475 2315 100-101 -5.358090494715425 2316 1 -1.5861268377569004 2316 2 1.5008161519836811 2316 3 1.6369503600504025 2316 4 1.8472785504356821 2316 5 -1.263126196383876 2316 6 -2.368374227489685 2316 7 -2.501431235298938 2316 8 -3.7939025013221226 2316 9 -2.9553980957807298 2316 10-11 -2.710984984504531 2316 12-13 -3.47675475378475 2316 14-15 -2.261469191223913 2316 16-17 -2.4577720796688745 2316 18-19 -4.128108956105869 2316 20-21 -1.646174557657016 2316 22-23 -2.1731450265827554 2316 24-25 -2.1595701602875046 2316 26-27 -2.603677158281247 2316 28-29 -1.7205275422372068 2316 30-31 0.7763170113082509 2316 32-33 -1.538261549778717 2316 34-35 -6.925674189991771 2316 36-37 -2.851260733121105 2316 38-39 -1.1219974963031412 2316 40-41 -0.9579683313252865 2316 42-43 -1.150131493425171 2316 44-45 -0.449935058125174 2316 46-47 0.41670698102977255 2316 48-49 0.6637193098190188 2316 50-51 1.0661398432714293 2316 52-53 0.8596009418582042 2316 54-55 -1.0638002276589482 2316 56-57 0.2655510729896555 2316 58-59 -3.186644419618002 2316 60-61 -3.3140138098776326 2316 62-63 -4.671777206814387 2316 64-65 -5.021969898612728 2316 66-67 -2.7401274653266263 2316 68-69 -2.412897087829922 2316 70-71 -3.003951226251889 2316 72-73 -2.863659875700801 2316 74-75 -2.6444098679877683 2316 76-77 -2.3571287028253316 2316 78-79 -2.7417374155084495 2316 80-81 -2.864510541925803 2316 82-83 -3.856655994799116 2316 84-85 -2.926233589768419 2316 86-87 -2.5098898340987006 2316 88-89 -3.6803151762367214 2316 90-91 -4.095748966956563 2316 92-93 -4.057164238826015 2316 94-95 -5.2829838023457665 2316 96-97 -4.975811159152428 2316 98-99 -5.245850832627173 2316 100-101 -5.16945322559838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39439.0 3 15141.0 4 13842.0 5 15097.0 6 17700.0 7 21975.0 8 27161.0 9 32666.0 10 38605.0 11 43961.0 12 48812.0 13 52834.0 14 57624.0 15 61902.0 16 65115.0 17 65476.0 18 62981.0 19 64400.0 20 67747.0 21 72873.0 22 79163.0 23 85994.0 24 92888.0 25 100733.0 26 109295.0 27 117258.0 28 125742.0 29 135537.0 30 144133.0 31 153343.0 32 162204.0 33 170138.0 34 172296.0 35 166968.0 36 141879.0 37 81920.0 38 15140.0 39 97.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.7313726754597 18.465829975063652 14.542908021794327 22.259889327682323 2 16.040629674299673 20.275562587218776 39.43890298625947 24.244904752222077 3 19.926279338471232 24.846884555229547 29.439266295717026 25.787569810582195 4 13.010240401917505 16.48005181782658 36.660632003202934 33.84907577705298 5 13.831710240237765 37.48421819331444 33.34415546808515 15.339916098362643 6 31.959088238157847 35.59740663850973 17.140949687234666 15.302555436097757 7 28.65757232108721 33.27871369561598 20.147258537907312 17.9164554453895 8 27.230232691954775 33.21118166165542 19.443599873206495 20.11498577318331 9 27.38992636847494 16.594244913425847 20.23860876694177 35.77721995115745 10-11 25.49635544234688 26.331942014989156 26.32203202810908 21.849670514554884 12-13 27.017527419107 23.949763965191842 27.075235535245362 21.957473080455795 14-15 23.755108151303816 25.093532660039457 26.16904716657393 24.9823120220828 16-17 22.225055397272232 28.289716244090474 26.75707823773183 22.72815012090546 18-19 22.211963084809135 26.32386281036586 29.97231394730866 21.491860157516342 20-21 23.301370768027855 25.106846484333495 30.018736871929974 21.573045875708676 22-23 22.79960258598404 25.129249814627546 30.180559751578773 21.89058784780964 24-25 22.60267988001839 25.34569825452337 30.33952152810151 21.712100337356734 26-27 22.11508214082369 25.569889912885095 30.415940482072457 21.89908746421875 28-29 21.958050040340773 26.107516241029753 30.25773471722391 21.67669900140556 30-31 22.102585132248297 25.620131378561716 30.039851076810475 22.23743241237951 32-33 21.869474566397187 25.76029966374702 30.07801614150561 22.29220962835018 34-35 22.73923095591898 25.799823727023842 29.75700727658252 21.70393804047466 36-37 22.747852492552713 25.39583905584771 29.933707101151462 21.922601350448115 38-39 22.280358284016042 25.879400888590524 29.85543629562614 21.984804531767292 40-41 22.44663745789341 25.90280463138014 29.803999493946172 21.846558416780283 42-43 22.292284872784695 25.951503182702606 29.249056546586754 22.507155397925942 44-45 22.249932866112125 25.907516492288842 29.523176559315452 22.319374082283584 46-47 22.894149082465297 26.354375905251803 28.74738269937679 22.004092312906113 48-49 22.664990890993053 26.541632998995762 28.465663744789744 22.327712365221444 50-51 22.49322483991986 26.770182515802745 28.582019340954812 22.154573303322582 52-53 22.74438886108869 26.827627250644586 28.123820656869526 22.304163231397194 54-55 22.309723986095886 26.835887225518633 28.203212557727937 22.65117623065754 56-57 22.492275362113958 26.96283129588433 27.957236309281196 22.58765703272051 58-59 22.682417141328358 26.51315709926828 28.0574488379495 22.746976921453857 60-61 22.30792451879292 26.840535896070744 28.563620845033878 22.287918740102462 62-63 22.232903876350715 26.850119819553832 28.656590722489604 22.260385581605853 64-65 21.99631038090592 27.065901500621038 28.325037223948986 22.612750894524055 66-67 22.16092930952172 27.023091764343732 27.95406300502806 22.861915921106487 68-69 22.253841485059258 26.78619372270143 28.298782455026256 22.661182337213052 70-71 22.42046279012073 26.669848489930857 27.92502838866306 22.984660331285355 72-73 22.33263783587895 26.88920201033505 27.869642251368177 22.908517902417824 74-75 22.064489999461806 27.666855602433728 27.520129493668037 22.748524904436426 76-77 22.38436236382508 26.978353232092605 27.64833614096999 22.98894826311232 78-79 22.286607511546425 27.19990201382709 27.59963176823476 22.913858706391725 80-81 22.53624199385009 27.289023395784668 27.286570382487803 22.88816422787744 82-83 22.55162541357564 27.103597631104158 27.497107695913574 22.84766925940663 84-85 22.25510605362061 27.43276815797501 27.334599219860102 22.97752656854428 86-87 22.351141865919104 27.404662839157766 27.26549979306927 22.97869550185386 88-89 22.26020450826952 27.366218174058364 26.916845645322013 23.456731672350102 90-91 22.152198516694217 27.346885594357 27.05608340167372 23.444832487275065 92-93 22.46686754733761 27.21812742269804 27.183674678902303 23.131330351062047 94-95 22.24266152950161 26.902573682888654 27.33413916760069 23.520625620009046 96-97 22.21438776962052 26.73904542174413 27.35485838772532 23.691708420910036 98-99 22.15437489966061 27.197914099597348 26.72034121016718 23.92736979057486 100-101 22.150764593033585 26.945865660869334 27.011409999355635 23.89195974674145 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4228.0 1 2859.0 2 1506.5 3 1529.0 4 1457.5 5 1440.5 6 1865.0 7 3214.5 8 4961.5 9 5837.5 10 5526.0 11 4854.0 12 4554.0 13 4547.5 14 4751.5 15 5080.0 16 5473.5 17 5837.5 18 6254.5 19 6824.0 20 7556.5 21 8583.0 22 9904.0 23 11459.5 24 13298.5 25 15598.0 26 18527.0 27 21722.5 28 24827.0 29 28684.0 30 33054.0 31 38187.5 32 44794.0 33 49532.0 34 51924.5 35 55582.5 36 61448.0 37 67131.0 38 70797.0 39 73318.5 40 75216.0 41 77695.5 42 79877.0 43 83265.5 44 88395.0 45 93843.5 46 99767.0 47 107209.5 48 115292.5 49 115895.0 50 110571.5 51 106568.0 52 104908.0 53 104567.5 54 103224.5 55 98495.5 56 91739.5 57 84212.5 58 76797.0 59 69039.5 60 59206.5 61 48937.5 62 39537.0 63 30607.5 64 22854.5 65 17085.0 66 12621.5 67 9466.0 68 7158.5 69 5334.0 70 3937.5 71 2781.0 72 1995.5 73 1500.5 74 1112.0 75 847.5 76 640.5 77 437.0 78 298.5 79 230.0 80 164.0 81 108.0 82 65.0 83 43.0 84 34.0 85 22.5 86 14.5 87 12.5 88 7.5 89 6.5 90 5.5 91 3.5 92 2.5 93 2.5 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.003055700203974 2 2.312556907484459 3 0.9433079859418744 4 0.8588884856495351 5 0.1611181196151532 6 0.09295668585776097 7 0.09509948542199036 8 0.10271832831702822 9 0.09816062765660377 10-11 1.66852659401329 12-13 1.2768704514402505 14-15 0.4885242879528067 16-17 0.09047375937857452 18-19 0.09727629767771546 20-21 0.12322798128893817 22-23 0.1393840097493979 24-25 0.15178163579958226 26-27 0.14824431588402895 28-29 0.531227222125664 30-31 0.1795019793685816 32-33 0.16288677957292985 34-35 0.14596546555381676 36-37 0.13409503622181582 38-39 0.39143506007831763 40-41 0.12355110185814736 42-43 0.12446944452853138 44-45 0.12865300558250306 46-47 0.1389928637971973 48-49 0.1538394036350724 50-51 0.16695129620666657 52-53 0.1852841369228514 54-55 0.18394063560877105 56-57 0.15321016884240185 58-59 0.13742827998839488 60-61 0.1165785001015279 62-63 0.12176543555462287 64-65 0.11773493161238184 66-67 0.11431665611706353 68-69 0.10974194911089123 70-71 0.12270078457075474 72-73 0.13778541324909976 74-75 0.14751304301687132 76-77 0.16766556272807637 78-79 0.1690770894251481 80-81 0.16712135966414507 82-83 0.15996168810429923 84-85 0.1470538716816793 86-87 0.1467307511124701 88-89 0.14310839946817755 90-91 0.1498769250758228 92-93 0.1419519679573236 94-95 0.19203565618474877 96-97 0.1954539316800671 98-99 0.2138037787419998 100-101 0.23638820589514772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2940079.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.217417446508634 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76713920227171 42.941005889525826 2 11.217219540697156 12.387717879356174 3 4.256515898029074 7.0510144562751575 4 2.153465026841409 4.756351093742359 5 1.2242013016506526 3.379861715590166 6 0.7486844923117904 2.4804254488624506 7 0.5279734153543479 2.040732993339595 8 0.3690374996822719 1.6301838138697444 9 0.28172764078694335 1.4000645472797417 >10 1.3485825632556292 12.93099970893902 >50 0.07158428627590571 2.703739611771851 >100 0.029954542644208035 3.046477011346975 >500 0.0021231675655249294 0.8088173168456659 >1k 0.0015923756740810937 1.4007117123723063 >5k 1.3269797284009115E-4 0.5724039388513826 >10k+ 6.634898642004558E-5 0.46949286203152 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12815 0.4358726415174558 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8875 0.30186263702437927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6749 0.22955165490451107 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4087 0.13900987014294514 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.401269149570471E-5 2 0.0 0.0 0.0 0.0 3.401269149570471E-5 3 0.0 0.0 0.0 0.0 3.401269149570471E-5 4 0.0 3.401269149570471E-5 0.0 0.0 3.401269149570471E-5 5 3.401269149570471E-5 3.401269149570471E-5 0.0 0.0 3.401269149570471E-5 6 3.401269149570471E-5 3.401269149570471E-5 0.0 0.0 3.401269149570471E-5 7 3.401269149570471E-5 3.401269149570471E-5 0.0 0.0 3.401269149570471E-5 8 3.401269149570471E-5 3.401269149570471E-5 0.0 0.0 3.401269149570471E-5 9 3.401269149570471E-5 3.401269149570471E-5 0.0 0.0 3.401269149570471E-5 10-11 3.401269149570471E-5 3.401269149570471E-5 0.0 5.101903724355707E-5 3.401269149570471E-5 12-13 3.401269149570471E-5 3.401269149570471E-5 0.0 1.0203807448711414E-4 5.101903724355707E-5 14-15 3.401269149570471E-5 3.401269149570471E-5 0.0 2.3808884046993295E-4 6.802538299140942E-5 16-17 3.401269149570471E-5 3.401269149570471E-5 0.0 2.721015319656377E-4 6.802538299140942E-5 18-19 5.101903724355707E-5 6.802538299140942E-5 0.0 3.2312056920919474E-4 6.802538299140942E-5 20-21 6.802538299140942E-5 6.802538299140942E-5 0.0 7.142665214097989E-4 6.802538299140942E-5 22-23 1.3605076598281884E-4 6.802538299140942E-5 0.0 0.0010713997821146983 6.802538299140942E-5 24-25 3.5713326070489944E-4 6.802538299140942E-5 0.0 0.001496558425811007 6.802538299140942E-5 26-27 3.741396064527518E-4 6.802538299140942E-5 0.0 0.0020407614897422826 1.1904442023496649E-4 28-29 4.931840266877183E-4 6.802538299140942E-5 0.0 0.0039964912507453034 1.3605076598281884E-4 30-31 5.442030639312754E-4 6.802538299140942E-5 0.0 0.008809287097387519 1.530571117306712E-4 32-33 5.782157554269801E-4 6.802538299140942E-5 0.0 0.01753354246603578 1.7006345747852354E-4 34-35 6.802538299140942E-4 1.0203807448711412E-4 0.0 0.03142772694203115 1.7006345747852354E-4 36-37 8.333109416447653E-4 1.0203807448711412E-4 0.0 0.05243056394062881 1.7006345747852354E-4 38-39 8.503172873926177E-4 1.0203807448711412E-4 0.0 0.0840623670316342 1.7006345747852354E-4 40-41 8.503172873926177E-4 1.0203807448711412E-4 0.0 0.12118721979919586 1.7006345747852354E-4 42-43 9.013363246361747E-4 1.0203807448711412E-4 0.0 0.15846512967848825 1.7006345747852354E-4 44-45 0.0010203807448711413 1.0203807448711412E-4 0.0 0.20416118070296752 1.7006345747852354E-4 46-47 0.0010884061278625507 1.0203807448711412E-4 0.0 0.2614215468359864 1.7006345747852354E-4 48-49 0.0011224188193582553 1.0203807448711412E-4 0.0 0.3225253471080199 1.7006345747852354E-4 50-51 0.0011224188193582553 1.0203807448711412E-4 0.0 0.39090786336013417 1.7006345747852354E-4 52-53 0.0011394251651061077 1.0203807448711412E-4 0.0 0.45799789733541174 2.0407614897422825E-4 54-55 0.0011564315108539601 1.0203807448711412E-4 0.0 0.5346114849294865 2.0407614897422825E-4 56-57 0.0012244568938453695 1.0203807448711412E-4 0.0 0.627057980414812 2.210824947220806E-4 58-59 0.0012244568938453695 1.0203807448711412E-4 0.0 0.7301844610297886 2.3808884046993295E-4 60-61 0.0012244568938453695 1.0203807448711412E-4 0.0 0.8423923302741185 2.3808884046993295E-4 62-63 0.0012244568938453695 1.0203807448711412E-4 0.0 0.952117273039262 2.8910787771349003E-4 64-65 0.0012584695853410741 1.3605076598281884E-4 0.0 1.0703453886783314 3.401269149570471E-4 66-67 0.0012754759310889265 1.3605076598281884E-4 0.0 1.2054267929535225 3.401269149570471E-4 68-69 0.001292482276836779 1.3605076598281884E-4 0.0 1.3581097650777412 3.401269149570471E-4 70-71 0.0013605076598281881 1.3605076598281884E-4 0.0 1.5294316921416056 3.401269149570471E-4 72-73 0.001394520351323893 1.3605076598281884E-4 0.0 1.7063657133022616 3.9114595220060414E-4 74-75 0.001394520351323893 1.3605076598281884E-4 0.0 1.8884526572245166 4.421649894441612E-4 76-77 0.001394520351323893 1.3605076598281884E-4 0.0 2.084637861771742 4.421649894441612E-4 78-79 0.0014115266970717453 1.3605076598281884E-4 0.0 2.3137643580325564 4.421649894441612E-4 80-81 0.0017006345747852354 1.3605076598281884E-4 0.0 2.561563821924513 4.421649894441612E-4 82-83 0.0017006345747852354 1.3605076598281884E-4 0.0 2.8174753127381953 5.101903724355707E-4 84-85 0.0017176409205330876 1.3605076598281884E-4 0.0 3.0772642503823877 5.101903724355707E-4 86-87 0.00173464726628094 1.3605076598281884E-4 0.0 3.346066551272942 5.442030639312754E-4 88-89 0.00173464726628094 1.3605076598281884E-4 0.0 3.637147165093183 5.782157554269801E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 20420 0.0 31.142603 1 GTACATG 13365 0.0 25.946938 1 GGTATCA 14220 0.0 25.444073 1 TATCAAC 24555 0.0 25.28573 2 TACATGG 13535 0.0 25.280642 2 ATCAACG 25605 0.0 24.334919 3 CAACGCA 26035 0.0 24.06768 5 TCAACGC 26075 0.0 24.001883 4 AACGCAG 26535 0.0 23.466259 6 ACATGGG 14250 0.0 22.672745 3 GAGTACT 12645 0.0 22.031374 12-13 AGAGTAC 19470 0.0 21.911575 10-11 AGTACTT 13400 0.0 21.793713 12-13 ACGCAGA 29165 0.0 21.085255 7 CGCAGAG 29420 0.0 20.755817 8 GTATCGA 270 1.24437065E-8 20.416342 1 GTACTTT 13805 0.0 20.1772 14-15 CATGGGA 7705 0.0 19.734356 4 GCAGAGT 28960 0.0 19.73039 9 TACTTTT 15290 0.0 19.086523 14-15 >>END_MODULE