FastQCFastQC Report
Fri 27 May 2016
SRR939416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940079
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173590.590426311673938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145410.49457854703904214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107880.36692891585566234No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62520.21264734723114584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41550.14132273316465305No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37960.12911217691769508No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.12261575284201547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.1089766635522379No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT29590.10064355413579022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA168050.026.9650631
GTATCAA231200.026.352011
TAAGGTG15500.024.4670165
GTAAGGT14100.023.5523474
GTACATG154600.023.3567141
TACATGG153300.022.8378222
TATCAAC282000.021.583312
ATCAACG285450.021.2035873
ACATGGG157150.021.1890493
TCAACGC286000.021.132884
GAGTACT153450.021.11192512-13
CAACGCA288650.020.8899925
AACGCAG294300.020.585596
AGAGTAC236350.020.13573810-11
GTACTTT163050.019.83983614-15
AGTACTT162100.019.8245312-13
AGGTAAG15100.019.4784872
GGTAAGG16000.019.2700163
CATGGGA85350.019.1209834
GTATAGG7550.018.854321
Produced by FastQC (version 0.11.5)