##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939416_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2940079 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.885637086622502 31.0 31.0 31.0 28.0 34.0 2 31.135576969190282 31.0 31.0 34.0 28.0 34.0 3 31.137290868714754 31.0 31.0 34.0 28.0 34.0 4 35.05671344205377 35.0 35.0 37.0 32.0 37.0 5 34.627180426104196 35.0 35.0 37.0 31.0 37.0 6 34.69088245587959 35.0 35.0 37.0 31.0 37.0 7 34.526262729674954 35.0 35.0 37.0 30.0 37.0 8 34.425399453552096 35.0 35.0 37.0 30.0 37.0 9 35.92339899710178 37.0 35.0 39.0 30.0 39.0 10-11 35.71922795271828 37.0 35.0 39.0 30.0 39.0 12-13 35.704121385853924 37.0 35.0 39.0 30.0 39.0 14-15 36.51215953720971 38.0 35.0 40.0 30.0 41.0 16-17 36.43779163757164 38.0 35.0 40.0 30.0 41.0 18-19 36.45721407485989 38.0 35.0 40.0 30.0 41.0 20-21 36.32906445711153 38.0 34.5 40.0 29.5 41.0 22-23 36.162196491999026 38.0 34.0 40.0 29.0 41.0 24-25 35.9888650951216 38.0 34.0 40.0 29.0 41.0 26-27 35.501927499227065 38.0 34.0 40.0 27.0 41.0 28-29 35.289507186711646 38.0 33.5 40.0 27.0 41.0 30-31 34.968165141140766 37.5 33.0 40.0 26.5 41.0 32-33 34.681296488971896 37.0 33.0 40.0 25.0 41.0 34-35 34.89576011392891 37.5 33.0 40.0 26.0 41.0 36-37 34.718244475743674 37.5 33.0 40.0 25.0 41.0 38-39 34.50873292860498 37.0 33.0 40.0 25.0 41.0 40-41 34.336260012060905 37.0 33.0 40.0 24.5 41.0 42-43 34.133168020315104 37.0 33.0 40.0 24.0 41.0 44-45 33.70267669678264 37.0 32.0 40.0 23.0 41.0 46-47 33.24960349704889 37.0 31.0 40.0 21.5 41.0 48-49 32.802182526387895 36.5 31.0 39.0 19.5 40.5 50-51 32.51272652877695 36.0 31.0 39.0 18.0 40.0 52-53 32.37216789072674 36.0 31.0 39.0 18.0 40.0 54-55 32.01808488819518 35.5 30.5 39.0 15.5 40.0 56-57 31.493514119858684 35.0 29.5 39.0 12.5 40.0 58-59 31.00906608291818 35.0 29.0 38.0 9.0 40.0 60-61 30.609194684904725 34.0 28.0 38.0 8.0 40.0 62-63 30.42234256290392 34.0 28.0 38.0 7.5 40.0 64-65 29.94866124345638 34.0 28.0 37.5 6.5 40.0 66-67 29.032902517245283 33.5 27.0 36.5 2.0 39.0 68-69 25.555303275864354 31.0 15.5 35.0 2.0 38.5 70-71 24.212838668620808 29.0 11.5 35.0 2.0 38.0 72-73 25.237713510419276 30.0 17.0 34.5 2.0 37.0 74-75 26.00491687468262 31.0 21.0 35.0 2.0 37.0 76-77 25.682220613799835 30.5 21.0 34.0 2.0 36.0 78-79 26.82031639285883 31.5 24.5 34.5 2.0 36.0 80-81 26.847124345978457 32.0 25.0 35.0 2.0 36.0 82-83 26.52909020471899 32.0 24.5 34.5 2.0 36.0 84-85 26.17766359339324 32.0 24.0 34.0 2.0 35.0 86-87 25.762266762219653 31.5 23.0 34.0 2.0 35.0 88-89 25.28342146588578 31.0 20.5 34.0 2.0 35.0 90-91 24.697333473012122 31.0 18.5 34.0 2.0 35.0 92-93 24.137011454454118 31.0 16.5 34.0 2.0 35.0 94-95 23.285863407071716 30.0 6.0 34.0 2.0 35.0 96-97 22.62530462616821 30.0 2.0 34.0 2.0 35.0 98-99 21.790476888546195 29.5 2.0 34.0 2.0 35.0 100-101 20.395939530876554 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23742187484180022 1101 2 0.20128164315723396 1101 3 0.3381282106806047 1101 4 0.3486019967479521 1101 5 0.5412819760037948 1101 6 0.40844599561917505 1101 7 0.4706549511802436 1101 8 0.5833401216173115 1101 9 0.5900658146058646 1101 10-11 0.6893546551162544 1101 12-13 0.7053148300079286 1101 14-15 0.8155488358210334 1101 16-17 0.7698696264878109 1101 18-19 0.48208742407825866 1101 20-21 0.6582638178685158 1101 22-23 0.5515352670874805 1101 24-25 0.6737747466638311 1101 26-27 0.6801134357189014 1101 28-29 0.5728057589823266 1101 30-31 0.42459326936632635 1101 32-33 0.6635092673868357 1101 34-35 0.6531513654627688 1101 36-37 0.7911124778925114 1101 38-39 0.8743504117994902 1101 40-41 0.8302383025456308 1101 42-43 0.5034758626657236 1101 44-45 0.7176387412210445 1101 46-47 0.7207336847914192 1101 48-49 0.8351321226676092 1101 50-51 0.5487536337090191 1101 52-53 0.32110738711190123 1101 54-55 0.42597603905597126 1101 56-57 0.4593141261660527 1101 58-59 0.35173463701872265 1101 60-61 0.29997176605851195 1101 62-63 0.0945062117575226 1101 64-65 0.24261580058452736 1101 66-67 -0.07980524923035404 1101 68-69 -7.347092721560291 1101 70-71 -7.93449306468241 1101 72-73 -5.157095231603915 1101 74-75 -3.052368746546957 1101 76-77 -0.4254051190003487 1101 78-79 -0.02167526606145387 1101 80-81 -0.14659332625949872 1101 82-83 -0.4028520190524496 1101 84-85 -0.37450164118466134 1101 86-87 -0.3190674263830182 1101 88-89 -0.4077089645397649 1101 90-91 -0.5837222227717085 1101 92-93 -0.4261470614542944 1101 94-95 -0.46217180522922874 1101 96-97 -0.5296233129742056 1101 98-99 -0.499793851553207 1101 100-101 -0.5058570595872425 1102 1 0.27003525720755306 1102 2 0.306616096803765 1102 3 0.32719869104041166 1102 4 0.34571188136794007 1102 5 0.41681865094689385 1102 6 0.46681866923368887 1102 7 0.4682121783574402 1102 8 0.6182424549452037 1102 9 0.7053124196348861 1102 10-11 0.6183237100374654 1102 12-13 0.5974492495550052 1102 14-15 0.8109299260777121 1102 16-17 0.6856835517222208 1102 18-19 0.6321423932730497 1102 20-21 0.6246470626521798 1102 22-23 0.5599885780689888 1102 24-25 0.5399998816323119 1102 26-27 0.4516858031190054 1102 28-29 0.4990032979006145 1102 30-31 0.5168246188060408 1102 32-33 0.5863425911913822 1102 34-35 0.56773560913566 1102 36-37 0.6930611235114483 1102 38-39 0.6971928049681608 1102 40-41 0.6120518197437264 1102 42-43 0.502985300249442 1102 44-45 0.3197049057205916 1102 46-47 0.4773223736438226 1102 48-49 0.608948264366127 1102 50-51 0.6108994946077715 1102 52-53 0.22565994416211765 1102 54-55 0.06877224142564486 1102 56-57 0.04092564157001988 1102 58-59 0.21679389927274073 1102 60-61 0.4777654194774428 1102 62-63 0.2116242451926773 1102 64-65 0.11474576372172862 1102 66-67 -0.008738996589517711 1102 68-69 -6.95270423322081 1102 70-71 -7.791756332158208 1102 72-73 -1.8490674660774395 1102 74-75 0.03402426354266552 1102 76-77 0.5048730382273732 1102 78-79 0.1916879910688003 1102 80-81 0.10164610257134754 1102 82-83 -0.0017740238821879473 1102 84-85 -0.20727009606448732 1102 86-87 -0.24635673467469488 1102 88-89 -0.17311259037965954 1102 90-91 -0.31339889228339857 1102 92-93 -0.31811766242132933 1102 94-95 -0.2350775222977397 1102 96-97 -0.3512467870068967 1102 98-99 -0.16995358466547117 1102 100-101 -0.34209884270930857 1103 1 0.353076195549491 1103 2 0.3386507196167621 1103 3 0.3584294178232845 1103 4 0.34860337112676376 1103 5 0.4204937491388634 1103 6 0.35764096611426766 1103 7 0.40650745215884854 1103 8 0.4236699450448569 1103 9 0.5309121210014567 1103 10-11 0.5372386228413788 1103 12-13 0.41141667680368954 1103 14-15 0.5931167058680202 1103 16-17 0.5693207425392401 1103 18-19 0.6314114057945588 1103 20-21 0.4573079889432847 1103 22-23 0.5277508256912924 1103 24-25 0.603885651303159 1103 26-27 0.7810253961903797 1103 28-29 0.600668884252741 1103 30-31 0.5047570489355593 1103 32-33 0.6732156030068808 1103 34-35 0.5806188672020127 1103 36-37 0.6972047383960529 1103 38-39 0.64986818110021 1103 40-41 0.6747837323366426 1103 42-43 0.5478000947766617 1103 44-45 0.7857650239567064 1103 46-47 0.7568447579639468 1103 48-49 0.8599125592098389 1103 50-51 0.5445206328636942 1103 52-53 0.503477663146441 1103 54-55 0.3387722617132418 1103 56-57 0.4836552768801745 1103 58-59 0.5667676268346611 1103 60-61 0.4537497229636642 1103 62-63 0.18448692149189228 1103 64-65 0.11542691979015629 1103 66-67 -0.018238790670409344 1103 68-69 -6.669469979439199 1103 70-71 -7.718741017051251 1103 72-73 -1.219842066817531 1103 74-75 0.3567839549277423 1103 76-77 0.5318886363463093 1103 78-79 0.3328539091231413 1103 80-81 0.17183091750788648 1103 82-83 0.30084363403647174 1103 84-85 0.0568395472389831 1103 86-87 0.18753942731770934 1103 88-89 0.36164716389696494 1103 90-91 0.18346882954118726 1103 92-93 0.1924739847692436 1103 94-95 0.47269092825732884 1103 96-97 0.29459161822568447 1103 98-99 0.4775567721527807 1103 100-101 0.4874760864869785 1104 1 0.28948641199136915 1104 2 0.20699581119557564 1104 3 0.2656815287167973 1104 4 0.2909045538791659 1104 5 0.4519273483539763 1104 6 0.4207544598630264 1104 7 0.40136912562729066 1104 8 0.5088369304691085 1104 9 0.5286761059017948 1104 10-11 0.491424255139016 1104 12-13 0.4140737117187854 1104 14-15 0.7345284237689498 1104 16-17 0.5461218630173832 1104 18-19 0.4513674903459233 1104 20-21 0.4845844950442526 1104 22-23 0.555371814127632 1104 24-25 0.5781205375704985 1104 26-27 0.766754021539036 1104 28-29 0.6734374346583394 1104 30-31 0.5925944410476376 1104 32-33 0.47807151769853107 1104 34-35 0.42990561684498374 1104 36-37 0.6984749294544841 1104 38-39 0.6432454604462237 1104 40-41 0.6697823072877185 1104 42-43 0.44317195868207193 1104 44-45 0.6775453570590244 1104 46-47 0.654808453473656 1104 48-49 0.789531443311212 1104 50-51 0.5643730033988206 1104 52-53 0.45895233433454763 1104 54-55 0.4124500926639776 1104 56-57 0.4367756246175851 1104 58-59 0.6161968304297076 1104 60-61 0.5373403674092465 1104 62-63 0.2298547993355271 1104 64-65 0.3350875773095119 1104 66-67 0.30039183282860904 1104 68-69 -6.208521668599733 1104 70-71 -4.012089820888875 1104 72-73 0.4943631621314175 1104 74-75 1.0870488102668112 1104 76-77 1.2485091894996145 1104 78-79 0.7710004184155004 1104 80-81 0.5267527868766209 1104 82-83 0.4849185658849997 1104 84-85 0.30990875112948046 1104 86-87 0.28812406669118573 1104 88-89 0.39899681741523096 1104 90-91 0.1609846394338028 1104 92-93 0.2765238540951849 1104 94-95 0.4288271838196671 1104 96-97 0.2278534826175651 1104 98-99 0.45909557538167434 1104 100-101 0.48172813446948126 1105 1 0.12806170427264618 1105 2 0.15972492164446095 1105 3 0.1123028645033628 1105 4 0.126988047066817 1105 5 0.3834550061805757 1105 6 0.22048186707627337 1105 7 0.16834239518613003 1105 8 0.44720662628770924 1105 9 0.3956573447126317 1105 10-11 0.43652587846029434 1105 12-13 0.30405012478064464 1105 14-15 0.3576146300858838 1105 16-17 0.3407219439385685 1105 18-19 0.4797677201743724 1105 20-21 0.45024375952154116 1105 22-23 0.4515169743087455 1105 24-25 0.4312928715357458 1105 26-27 0.6192330135156894 1105 28-29 0.507544954372861 1105 30-31 0.29288055767209187 1105 32-33 0.5263398862697883 1105 34-35 0.5106569210634078 1105 36-37 0.5622418890722258 1105 38-39 0.5073975294739412 1105 40-41 0.6114652153582156 1105 42-43 0.4873093281087719 1105 44-45 0.5006884573506127 1105 46-47 0.45846310636495247 1105 48-49 0.5838325066346783 1105 50-51 0.5437164291840162 1105 52-53 0.3588651845059516 1105 54-55 0.4841622258757461 1105 56-57 0.548089266219332 1105 58-59 0.7556914194441191 1105 60-61 0.798862890443715 1105 62-63 0.46013798252440097 1105 64-65 0.3999739693936384 1105 66-67 0.35098813594892064 1105 68-69 -3.2430915841103065 1105 70-71 -3.364488309768525 1105 72-73 0.6317945339878044 1105 74-75 1.083649890160185 1105 76-77 1.0239100283700395 1105 78-79 0.6428174317616957 1105 80-81 0.4277785166391119 1105 82-83 0.4392214756176074 1105 84-85 0.43807381976487747 1105 86-87 0.4574976766582459 1105 88-89 0.5869465916737973 1105 90-91 0.5538488397961565 1105 92-93 0.4194155714143868 1105 94-95 0.2334545718009089 1105 96-97 0.3919847923410984 1105 98-99 0.4349183665208862 1105 100-101 0.5344314259070764 1106 1 0.1261083813439754 1106 2 0.18432004904991572 1106 3 0.19040345631523792 1106 4 0.21235569476733218 1106 5 0.25484585581327224 1106 6 0.2659774162427553 1106 7 0.3770160573046297 1106 8 0.434765735789739 1106 9 0.35343529228005366 1106 10-11 0.43306230341359964 1106 12-13 0.25748886364020507 1106 14-15 0.3413168729654572 1106 16-17 0.3683172275386184 1106 18-19 0.3351474662771281 1106 20-21 0.49309531000668017 1106 22-23 0.4695746811761907 1106 24-25 0.5152002689793846 1106 26-27 0.5956309773936042 1106 28-29 0.602101679636533 1106 30-31 0.48512123947129027 1106 32-33 0.6405906660405734 1106 34-35 0.5098666868409794 1106 36-37 0.4976005248611628 1106 38-39 0.6609453641128411 1106 40-41 0.5470825874028762 1106 42-43 0.4708143241095115 1106 44-45 0.6132406431755157 1106 46-47 0.6572840865225302 1106 48-49 0.7118294347482959 1106 50-51 0.5896574089996491 1106 52-53 0.3675909767927905 1106 54-55 0.3676840257076499 1106 56-57 0.44367656412587664 1106 58-59 0.6540890623839886 1106 60-61 0.807268724249461 1106 62-63 0.45419390553204053 1106 64-65 0.5797986948375602 1106 66-67 0.67986377887134 1106 68-69 -2.705521530300647 1106 70-71 -2.8047608417291983 1106 72-73 1.082535443132958 1106 74-75 1.6504176914906346 1106 76-77 1.5216553139915376 1106 78-79 1.0783392080842695 1106 80-81 0.8545718736848151 1106 82-83 0.710363535936505 1106 84-85 0.6978228914578395 1106 86-87 0.6985245020556654 1106 88-89 0.6743128890123806 1106 90-91 0.915521206585943 1106 92-93 0.876729610351898 1106 94-95 1.1894170466285559 1106 96-97 1.125505015978984 1106 98-99 1.1740004623638143 1106 100-101 1.3027478245049942 1107 1 0.04322828590775529 1107 2 0.14883286038323362 1107 3 0.17135006183112367 1107 4 -0.01324423347956838 1107 5 0.3620848108646939 1107 6 0.30895880748037996 1107 7 0.10532919764658999 1107 8 0.10128380927241665 1107 9 0.21986101615069487 1107 10-11 0.03578417866340544 1107 12-13 0.19229699866761507 1107 14-15 0.20491038588809118 1107 16-17 0.13793611757622415 1107 18-19 0.21271849171544233 1107 20-21 0.27423084114221297 1107 22-23 0.29738444059292846 1107 24-25 0.3057140954126112 1107 26-27 0.5054379267353752 1107 28-29 0.4151910562374539 1107 30-31 0.319422436516561 1107 32-33 0.48725144061457826 1107 34-35 0.226131300894572 1107 36-37 0.37043836972834043 1107 38-39 0.3588842982426499 1107 40-41 0.36569352990439086 1107 42-43 0.05388422486763034 1107 44-45 0.34031091723336004 1107 46-47 0.5847457993946037 1107 48-49 0.5000543619911824 1107 50-51 0.5988096590676335 1107 52-53 0.42031291897647094 1107 54-55 0.31634723208465587 1107 56-57 0.5508146880881633 1107 58-59 0.7033873850977841 1107 60-61 0.6309865295376937 1107 62-63 0.4073514341959914 1107 64-65 0.27261073383055034 1107 66-67 0.5630295671744356 1107 68-69 -2.589033653553816 1107 70-71 -3.041579364127756 1107 72-73 1.0692915257958973 1107 74-75 1.38823654052646 1107 76-77 1.1098324122057015 1107 78-79 1.051998914776238 1107 80-81 0.7958969768299049 1107 82-83 0.5942203686029863 1107 84-85 0.5806224080805755 1107 86-87 0.5065889620466173 1107 88-89 0.5366496229686177 1107 90-91 0.46083313944094684 1107 92-93 0.5739873011488008 1107 94-95 0.9357482579670773 1107 96-97 0.8602026431925331 1107 98-99 0.9709673350720465 1107 100-101 1.2858849842371214 1108 1 -0.057773935914138264 1108 2 -0.05350625769359141 1108 3 -0.019176970335252008 1108 4 -0.02148719270613242 1108 5 -0.03806891519067079 1108 6 -0.18509932018429964 1108 7 0.03184974449500544 1108 8 0.17174278563302892 1108 9 0.06504786937033202 1108 10-11 0.1673126319951379 1108 12-13 -0.014932529545518491 1108 14-15 0.11965677792956342 1108 16-17 0.2922460517222234 1108 18-19 0.3296829025323049 1108 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100-101 0.3610722066658383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 33.0 4 87.0 5 295.0 6 711.0 7 1639.0 8 3678.0 9 6164.0 10 9446.0 11 13728.0 12 17783.0 13 22101.0 14 26952.0 15 33624.0 16 39952.0 17 44521.0 18 41386.0 19 36743.0 20 35744.0 21 36123.0 22 37803.0 23 41392.0 24 49310.0 25 59998.0 26 74688.0 27 93088.0 28 112001.0 29 132916.0 30 155063.0 31 179752.0 32 206633.0 33 237873.0 34 273078.0 35 300969.0 36 298263.0 37 227371.0 38 84655.0 39 4510.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.099635508060395 19.94019937171578 15.494003267295678 22.46616185292815 2 16.21712737555145 21.42891647910821 38.03171801731434 24.322238128025997 3 19.663157336210364 24.902323654528963 30.22223643202689 25.212282577233786 4 13.39440390661036 17.347106551810416 36.14343074328062 33.115058798298605 5 13.566268117285285 38.07363679683437 33.39991884571809 14.960176240162253 6 31.996793283445786 36.471026798939754 16.43751749527819 15.094662422336272 7 28.196385199173218 33.52923509878476 20.015482577168843 18.258897124873176 8 27.188146985166046 34.07513879729082 19.036495277847976 19.70021893969516 9 26.634318329541486 16.486869910638454 20.05980111418775 36.81901064563231 10-11 25.368939423317 26.692491745975378 26.224293126895976 21.714275703811648 12-13 26.47008804865448 24.15569445582925 27.22508476813038 22.149132727385897 14-15 23.131436264127597 25.41804148800083 26.14715454924851 25.303367698623063 16-17 21.929853203825907 28.54685426808881 26.815802926671978 22.707489601413304 18-19 21.815248684855646 26.6045628404567 29.926500211814215 21.653688262873434 20-21 22.913806057592332 25.408892073988486 29.999159886520054 21.678141981899127 22-23 22.422895621979457 25.37166594162798 30.19283874924194 22.012599687150626 24-25 22.355373777218208 25.695411835474996 30.164085203011666 21.785129184295126 26-27 21.94913387795294 25.85229963885717 30.20438116342925 21.994185319760646 28-29 21.817726660568113 26.347987383167 30.06931367101916 21.764972285245733 30-31 22.04942225064178 25.861386071261848 29.75880696819551 22.330384709900862 32-33 21.857499264469542 25.947031603150283 29.847791963075522 22.347677169304657 34-35 22.487485108405707 26.01260715357751 29.653239501053996 21.846668236962785 36-37 22.548281502106686 25.62959276198329 29.894726163255726 21.927399572654302 38-39 22.175726020821152 26.05163325426028 29.751928522729887 22.02071220218868 40-41 22.291154461830686 26.107004962270235 29.69653645281503 21.905304123084047 42-43 22.132294506517265 26.17112693952381 29.095218812855432 22.601359741103497 44-45 22.153643265132995 26.070842202622508 29.404719068917824 22.370795463326672 46-47 22.7213170447919 26.4950178836039 28.752076094589917 22.031588977014284 48-49 22.555470759393877 26.684138004278086 28.384870261872635 22.3755209744554 50-51 22.38065915482602 26.886504080316918 28.522222017985122 22.210614746871936 52-53 22.811813316075956 26.79139579353158 28.060582102914296 22.33620878747817 54-55 22.3402039718323 26.858769805308274 28.182453004771414 22.618573218088013 56-57 22.55941783332829 26.922946067112203 27.881837639624298 22.635798459935213 58-59 22.69168177889577 26.57241035702397 27.936023602123772 22.799884261956493 60-61 22.297585985900447 26.838728046840664 28.533851441727432 22.329834525531457 62-63 22.24715034789021 26.89113430983861 28.582031069303216 22.279684272967966 64-65 21.99668183453222 27.03291208562262 28.290702588798343 22.679703491046823 66-67 21.96227669830063 26.971842178016548 28.22871776579829 22.83716335788453 68-69 22.112325674995635 26.461915834646522 28.92003268510838 22.505725805249458 70-71 21.81174061274116 26.294399568310357 29.160213290933278 22.733646528015203 72-73 22.23150802453773 26.747395110547135 28.255808832641506 22.765288032273627 74-75 22.02612468422946 27.56352250015059 27.59935775598471 22.810995059635243 76-77 21.567122122315734 27.048779294902918 28.303286121113974 23.080812461667374 78-79 22.17660642875962 27.12056318278761 27.780046566776072 22.922783821676703 80-81 22.475653349755195 27.239659678362138 27.382024422926182 22.90266254895648 82-83 22.450391493935413 27.138999054428204 27.596308868647153 22.81430058298923 84-85 22.088327323567032 27.442798662823453 27.441523185373978 23.027350828235534 86-87 22.20782136206325 27.456892373364173 27.35412113842569 22.981165126146884 88-89 21.997306865927296 27.40447588134836 27.05047058419377 23.54774666853057 90-91 21.970587264889787 27.32909494069799 27.210759635109564 23.489558159302653 92-93 22.195443469914043 27.24742677090255 27.352071959426304 23.205057799757103 94-95 21.902405366333042 26.82866968737352 27.681200712252313 23.587724234041126 96-97 21.750660106433656 26.67042655125012 27.86210580757557 23.716807534740653 98-99 21.761710731943282 27.08073807325624 27.21277742210478 23.944773772695697 100-101 21.56121600455016 26.654820692004716 27.60108127543966 24.182882028005462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3374.0 1 2358.0 2 1387.0 3 1495.0 4 1433.5 5 1384.5 6 1987.5 7 3682.5 8 5727.0 9 6804.0 10 6233.5 11 5217.0 12 4938.0 13 5005.0 14 5204.5 15 5340.5 16 5674.5 17 6135.5 18 6526.0 19 6992.0 20 7583.5 21 8520.0 22 9757.0 23 11282.0 24 13046.5 25 15334.0 26 18226.0 27 21504.5 28 24709.5 29 28383.5 30 32847.0 31 38515.5 32 46021.5 33 50422.0 34 52039.5 35 55636.5 36 61564.0 37 67489.5 38 71051.0 39 72613.5 40 74072.0 41 76631.0 42 78509.0 43 81794.5 44 87577.0 45 93626.0 46 99049.0 47 107791.5 48 118049.0 49 116907.5 50 108819.5 51 104451.0 52 103328.5 53 104837.0 54 105357.0 55 101040.5 56 93817.5 57 85643.0 58 78718.0 59 70915.5 60 59735.0 61 49073.0 62 39483.5 63 29619.5 64 21472.5 65 15725.0 66 11328.0 67 8465.5 68 6396.5 69 4662.0 70 3333.5 71 2301.5 72 1606.5 73 1244.0 74 951.0 75 712.5 76 487.5 77 291.5 78 189.5 79 122.0 80 77.0 81 50.0 82 28.5 83 16.0 84 8.5 85 3.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021938186014729535 2 0.018740993014133293 3 4.081522979484565E-4 4 0.08482765259028754 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010237820140207117 12-13 0.0 14-15 0.0 16-17 5.442030639312753E-4 18-19 8.503172873926177E-5 20-21 0.0 22-23 2.0407614897422825E-4 24-25 0.00382642779326678 26-27 0.0036223516442925514 28-29 0.0013094886225846313 30-31 2.210824947220806E-4 32-33 8.16304595896913E-4 34-35 0.0027720343568999337 36-37 0.0899465626603911 38-39 0.09936807820470131 40-41 0.0820216055418919 42-43 0.08620516659586358 44-45 0.0853038302712274 46-47 0.08407937337738204 48-49 0.07873938081255641 50-51 0.07880740619554781 52-53 0.06185207948493901 54-55 0.05224349413740243 56-57 0.02897881315434041 58-59 0.023213661945818462 60-61 0.013979216204734635 62-63 0.0024149010961950343 64-65 0.006853557336384499 66-67 0.16273372246119916 68-69 0.7459153308465521 70-71 0.9140910839470641 72-73 0.3699220327072844 74-75 0.055508712520990086 76-77 0.20603187873523124 78-79 0.014608450997405173 80-81 0.0032482120378397998 82-83 0.002006748798246578 84-85 1.1904442023496649E-4 86-87 0.0018366853407680542 88-89 3.401269149570471E-4 90-91 0.004353624511450202 92-93 0.00491483392112933 94-95 0.08013390116388029 96-97 0.03942070944352175 98-99 0.014455393885674501 100-101 0.013690108327021146 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2940079.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.43392139816704 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34442700681197 27.634331895961996 2 14.16942465804153 11.45850805761002 3 6.06043521078259 7.351414828544013 4 3.2610600615347485 5.27429784811191 5 1.973303385154674 3.989419698504051 6 1.3040948767393319 3.163780184509829 7 0.9281700867106146 2.6270689431130814 8 0.6503604328349593 2.103729809738131 9 0.483115498650019 1.7580828688786028 >10 2.56371164938844 18.51161823265864 >50 0.16892619874071027 4.689686575174347 >100 0.08365459787097766 5.805107635552175 >500 0.005641084631187209 1.5388205517195355 >1k 0.0033333681911212327 2.4028022660080497 >5k 8.547097925951879E-5 0.21606454711237832 >10k+ 2.564129377785564E-4 1.475266056803136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17359 0.590426311673938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14541 0.49457854703904214 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10788 0.36692891585566234 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6252 0.21264734723114584 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4155 0.14132273316465305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3796 0.12911217691769508 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3605 0.12261575284201547 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3204 0.1089766635522379 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2959 0.10064355413579022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.802538299140942E-5 2 6.802538299140942E-5 0.0 0.0 0.0 6.802538299140942E-5 3 6.802538299140942E-5 0.0 0.0 0.0 6.802538299140942E-5 4 6.802538299140942E-5 0.0 0.0 3.401269149570471E-5 6.802538299140942E-5 5 6.802538299140942E-5 0.0 0.0 3.401269149570471E-5 6.802538299140942E-5 6 6.802538299140942E-5 0.0 0.0 3.401269149570471E-5 6.802538299140942E-5 7 6.802538299140942E-5 0.0 0.0 3.401269149570471E-5 6.802538299140942E-5 8 6.802538299140942E-5 0.0 0.0 3.401269149570471E-5 6.802538299140942E-5 9 6.802538299140942E-5 0.0 0.0 6.802538299140942E-5 6.802538299140942E-5 10-11 6.802538299140942E-5 0.0 0.0 8.503172873926177E-5 6.802538299140942E-5 12-13 1.530571117306712E-4 0.0 0.0 1.530571117306712E-4 1.1904442023496649E-4 14-15 1.7006345747852354E-4 0.0 0.0 2.721015319656377E-4 1.870698032263759E-4 16-17 1.870698032263759E-4 0.0 0.0 3.061142234613424E-4 2.0407614897422825E-4 18-19 2.721015319656377E-4 0.0 0.0 3.5713326070489944E-4 2.3808884046993298E-4 20-21 3.401269149570471E-4 0.0 0.0 5.101903724355707E-4 3.061142234613424E-4 22-23 3.401269149570471E-4 0.0 0.0 8.503172873926177E-4 3.401269149570471E-4 24-25 4.421649894441612E-4 0.0 0.0 0.0013605076598281883 3.401269149570471E-4 26-27 4.421649894441612E-4 0.0 0.0 0.002074774181237987 3.401269149570471E-4 28-29 5.101903724355707E-4 0.0 0.0 0.004370630857198055 3.5713326070489944E-4 30-31 5.101903724355707E-4 0.0 0.0 0.00996571860824148 3.741396064527518E-4 32-33 5.101903724355707E-4 0.0 0.0 0.019982456253726515 3.741396064527518E-4 34-35 5.442030639312754E-4 0.0 0.0 0.03569631972474209 3.741396064527518E-4 36-37 6.122284469226848E-4 0.0 0.0 0.059760298957953165 3.741396064527518E-4 38-39 6.632474841662419E-4 0.0 0.0 0.0960858534753658 3.741396064527518E-4 40-41 6.802538299140942E-4 0.0 0.0 0.13708815307343783 3.741396064527518E-4 42-43 7.142665214097989E-4 0.0 0.0 0.17861764938969327 3.741396064527518E-4 44-45 7.822919044012083E-4 0.0 0.0 0.23031694046316442 3.9114595220060414E-4 46-47 7.822919044012083E-4 0.0 0.0 0.2931553880014789 4.2515864369630884E-4 48-49 8.16304595896913E-4 0.0 0.0 0.36090866946092265 4.761776809398659E-4 50-51 8.16304595896913E-4 0.0 0.0 0.43541347018226384 4.761776809398659E-4 52-53 8.16304595896913E-4 0.0 0.0 0.5085067442065332 4.761776809398659E-4 54-55 8.16304595896913E-4 0.0 0.0 0.5927561810413937 4.761776809398659E-4 56-57 8.16304595896913E-4 0.0 0.0 0.6928895448047485 5.271967181834231E-4 58-59 8.503172873926177E-4 3.401269149570471E-5 0.0 0.791645394562527 5.442030639312754E-4 60-61 8.503172873926177E-4 3.401269149570471E-5 0.0 0.8921188852408388 5.442030639312754E-4 62-63 8.503172873926177E-4 3.401269149570471E-5 0.0 0.9854497107050525 5.612094096791277E-4 64-65 8.843299788883224E-4 3.401269149570471E-5 0.0 1.0905829401182756 5.952221011748325E-4 66-67 0.0010203807448711413 3.401269149570471E-5 0.0 1.2018044413092301 6.122284469226848E-4 68-69 0.001054393436366846 3.401269149570471E-5 0.0 1.327702418880581 6.122284469226848E-4 70-71 0.0010713997821146983 3.401269149570471E-5 0.0 1.4886674813840037 6.122284469226848E-4 72-73 0.0011054124736104031 3.401269149570471E-5 0.0 1.6711285649127117 7.142665214097989E-4 74-75 0.0011224188193582553 3.401269149570471E-5 0.0 1.8643206526083143 8.16304595896913E-4 76-77 0.0011224188193582553 3.401269149570471E-5 0.0 2.0840426396705665 8.16304595896913E-4 78-79 0.0011394251651061077 3.401269149570471E-5 0.0 2.3440526598094813 8.16304595896913E-4 80-81 0.001394520351323893 3.401269149570471E-5 0.0 2.6284497797508166 8.16304595896913E-4 82-83 0.0014285330428195978 3.401269149570471E-5 0.0 2.9203637045127016 8.503172873926177E-4 84-85 0.0015305711173067118 3.401269149570471E-5 0.0 3.217498577419178 8.503172873926177E-4 86-87 0.001632609191793826 3.401269149570471E-5 0.0 3.526367828891672 9.013363246361747E-4 88-89 0.001632609191793826 3.401269149570471E-5 0.0 3.8657124519443187 9.523553618797318E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16805 0.0 26.965063 1 GTATCAA 23120 0.0 26.35201 1 TAAGGTG 1550 0.0 24.467016 5 GTAAGGT 1410 0.0 23.552347 4 GTACATG 15460 0.0 23.356714 1 TACATGG 15330 0.0 22.837822 2 TATCAAC 28200 0.0 21.58331 2 ATCAACG 28545 0.0 21.203587 3 ACATGGG 15715 0.0 21.189049 3 TCAACGC 28600 0.0 21.13288 4 GAGTACT 15345 0.0 21.111925 12-13 CAACGCA 28865 0.0 20.889992 5 AACGCAG 29430 0.0 20.58559 6 AGAGTAC 23635 0.0 20.135738 10-11 GTACTTT 16305 0.0 19.839836 14-15 AGTACTT 16210 0.0 19.82453 12-13 AGGTAAG 1510 0.0 19.478487 2 GGTAAGG 1600 0.0 19.270016 3 CATGGGA 8535 0.0 19.120983 4 GTATAGG 755 0.0 18.85432 1 >>END_MODULE