FastQCFastQC Report
Fri 27 May 2016
SRR939415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564107
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125540.8026305105724864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92520.59151963388694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77680.4966412144437689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69890.44683643766059483No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27130.17345360643485386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22820.1458979468795933No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20200.1291471747137504No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.11425049565023365No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA17740.11341935046643228No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT16720.10689805748583696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16530.10568330683258882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3650.040.4146589
ACCCGTG550.002298296735.369127
CCTTTAG4500.031.245691
GTATCAA146550.028.6118051
AACGCCG1604.9960363E-827.3404226
GGCATTC5550.027.1641247
ACTCCCG4300.027.128486
GGTATCA100900.027.024231
GTACATG63950.025.755971
GTGGTAC4800.025.1081451
CCCTATA5950.024.6478562
AACCCGT1000.001446591524.3025996
GAGTACT97900.024.16312612-13
CTTTAGG6300.024.0544832
TTAGGCA6100.023.555164
TATCAAC177600.023.50662
AACGCAG175800.023.445516
TCAACGC177700.023.3682544
CAACGCA176900.023.3160255
ATCAACG179350.023.3057863
Produced by FastQC (version 0.11.5)