FastQCFastQC Report
Fri 27 May 2016
SRR939414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565957
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126220.8060246865016089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86450.5520585814297583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76310.4873058455628092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70280.44879904109755253No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.17727178971070087No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.15607069670495421No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19010.12139541507206136No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18750.11973508851137037No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA17260.11022014014433347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.10568617145936957No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT16260.10383426875706038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG5000.036.8938759
CCTTTAG5600.034.240511
GGCATTC5950.032.6339387
TCGCGAC300.00402893131.5421690-91
GTATCGA906.286043E-428.0331631
GTATCAA147350.027.9095021
GCTAACG700.007547353427.7397568
GTACATG66100.027.1764771
CTTTAGG7550.026.598822
GGTATCA104200.025.3751261
TAGGCAT7700.025.2378655
ACATGGG68300.024.3877743
GTGTACG1251.4979087E-424.2206521
TTAGGCA8200.023.840634
GAGTACT92800.023.79531712-13
GGGGTCC4300.023.7046996
CATGGGT8050.023.6925534
TACATGG75150.023.5941892
CATGGGG16950.023.3483474
GGATATG6400.022.8645521
Produced by FastQC (version 0.11.5)