FastQCFastQC Report
Fri 27 May 2016
SRR939414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565957
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157731.007243493914584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121320.7747339167039708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120880.7719241332935707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103440.6605545362995281No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37830.24157751458054083No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.23704354589557694No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT26830.1713329293205369No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26340.1682038523407731No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA25820.1648831992193911No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.14847150975409926No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC20990.13403944041886207No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT19430.12407748105471607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.1237581874853524No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19290.12318345906049784No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA18450.11781932709518843No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC18360.11724459867033386No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA17920.1144348152599337No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA17200.10983698786109708No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16470.10517530174838773No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA16070.1026209531934785No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT15870.10134377891602388No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA15860.10127992020215115No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.10089676791891476No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA451.5211541E-552.7488374
CATTCCG5350.039.885459
CCTTTAG5650.036.964961
GGCATTC6250.034.141947
TAGGCAT6800.032.7751465
TTAGGCA6900.032.3373344
ACCGGCG300.003754019732.0008266-67
ATCACGC753.095833E-431.637163
CTTTAGG7400.029.5042322
GTACATG74700.028.2764821
CATGGGG17100.028.040174
GTGGTAC4250.027.921391
TATCACG856.4372236E-427.9196052
CGCATAG1400.027.42927666-67
ATCGACC350.00797472827.42927666-67
GGTCAAC2450.027.0967797
CATGGGT7100.026.745894
CCGCAAT458.4307697E-426.66088364-65
CACGCAG909.03821E-426.344095
GGTACTC4150.026.3007723
Produced by FastQC (version 0.11.5)