##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939414_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1565957 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.872670833234885 31.0 31.0 31.0 28.0 34.0 2 31.142839171190523 31.0 31.0 34.0 28.0 34.0 3 31.120800890445906 31.0 31.0 34.0 28.0 34.0 4 35.05513305920916 35.0 35.0 37.0 32.0 37.0 5 34.64790859519131 35.0 35.0 37.0 32.0 37.0 6 34.70210420848082 35.0 35.0 37.0 31.0 37.0 7 34.5468783625604 35.0 35.0 37.0 30.0 37.0 8 34.447467586913305 35.0 35.0 37.0 30.0 37.0 9 35.898136411153054 37.0 35.0 39.0 30.0 39.0 10-11 35.735643762887484 37.0 35.0 39.0 30.0 39.0 12-13 35.756383795979076 37.0 35.0 39.0 30.0 39.0 14-15 36.51856787893921 38.0 35.0 40.0 30.5 41.0 16-17 36.45207020371568 38.0 35.0 40.0 30.0 41.0 18-19 36.543895841328975 38.0 35.0 40.0 30.0 41.0 20-21 36.46448816921537 38.0 35.0 40.0 30.0 41.0 22-23 36.2825741702997 38.0 34.0 40.0 30.0 41.0 24-25 36.12639619095543 38.0 34.0 40.0 29.0 41.0 26-27 35.657952293709215 38.0 34.0 40.0 27.0 41.0 28-29 35.47049120761298 38.0 34.0 40.0 27.0 41.0 30-31 35.13087332538505 37.5 33.5 40.0 27.0 41.0 32-33 34.78508413704846 37.0 33.0 40.0 26.5 41.0 34-35 34.91266937725621 37.0 33.0 40.0 26.5 41.0 36-37 34.66547740455198 37.0 33.0 40.0 25.0 41.0 38-39 34.447687260889026 37.0 33.0 40.0 25.0 41.0 40-41 34.325398781703456 37.0 33.0 40.0 24.5 41.0 42-43 34.143033940267834 37.0 33.0 40.0 24.0 41.0 44-45 33.72393367123108 37.0 33.0 40.0 23.5 41.0 46-47 33.25642849707879 37.0 32.0 40.0 20.0 41.0 48-49 32.84629526864403 37.0 31.0 40.0 18.5 41.0 50-51 32.64416168515483 37.0 31.0 40.0 16.5 41.0 52-53 32.58830766106604 37.0 31.0 39.5 16.0 41.0 54-55 32.278102783154324 36.5 31.0 39.0 14.0 40.0 56-57 31.817555335172038 36.0 30.5 39.0 10.5 40.0 58-59 31.42043459686313 35.5 29.5 39.0 8.0 40.0 60-61 31.090669156305058 35.0 29.0 39.0 8.0 40.0 62-63 30.95390262951026 35.0 29.0 39.0 7.5 40.0 64-65 30.532275471165555 35.0 28.0 38.0 3.5 40.0 66-67 29.717887208908035 34.5 27.0 38.0 2.0 40.0 68-69 26.36467029426734 31.0 17.0 36.0 2.0 39.0 70-71 25.12664971004951 30.5 12.5 36.0 2.0 39.0 72-73 26.088176112115466 31.0 19.5 35.5 2.0 38.5 74-75 26.774721144961198 31.5 22.0 35.0 2.0 38.0 76-77 26.365567509197252 31.0 23.0 34.5 2.0 37.0 78-79 27.416217686692548 32.5 26.0 35.0 2.0 37.0 80-81 27.356710624876673 33.0 26.0 35.0 2.0 37.0 82-83 26.993426064700373 32.5 25.5 35.0 2.0 36.5 84-85 26.589867729445956 32.0 25.0 35.0 2.0 36.0 86-87 26.163424985488106 32.0 24.0 35.0 2.0 36.0 88-89 25.653724846850842 32.0 22.0 34.0 2.0 35.0 90-91 25.0589419121981 31.0 20.0 34.0 2.0 35.0 92-93 24.514312334246725 31.0 18.0 34.0 2.0 35.0 94-95 23.672368079072413 31.0 7.0 34.0 2.0 35.0 96-97 23.024436814037678 30.5 2.0 34.0 2.0 35.0 98-99 22.235985087713136 30.0 2.0 34.0 2.0 35.0 100-101 20.808455468445175 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.29327358292139394 1101 2 0.29917006874381613 1101 3 0.3988088995547976 1101 4 0.43127025574229805 1101 5 0.5773175818833494 1101 6 0.42309809511986884 1101 7 0.45262421472704517 1101 8 0.6903834695623701 1101 9 0.7343965322166923 1101 10-11 0.8120594441773576 1101 12-13 0.7987401211871017 1101 14-15 0.6993491176212103 1101 16-17 0.6971131623004752 1101 18-19 0.5992304202923293 1101 20-21 0.6351368922149945 1101 22-23 0.7395868427413674 1101 24-25 0.856386736304863 1101 26-27 0.9303515983206836 1101 28-29 0.8286145851899889 1101 30-31 0.5733797426792506 1101 32-33 1.0431122740411851 1101 34-35 1.0627808406884682 1101 36-37 0.9840583145343302 1101 38-39 1.1903559160515869 1101 40-41 1.0718423294547605 1101 42-43 0.8568655831097871 1101 44-45 0.7554935539869874 1101 46-47 0.9079580560579217 1101 48-49 0.8831513467060788 1101 50-51 0.7465818765940924 1101 52-53 0.5941875623226096 1101 54-55 0.6945104155307575 1101 56-57 0.8982647798694323 1101 58-59 0.7603613411272647 1101 60-61 0.9020645721781726 1101 62-63 0.5977315971818875 1101 64-65 0.4887568826559985 1101 66-67 0.3495535512470127 1101 68-69 -7.200169958838952 1101 70-71 -7.590644999696437 1101 72-73 -4.524800141065899 1101 74-75 -2.4078074320372878 1101 76-77 0.042523089117256774 1101 78-79 0.3525911973939557 1101 80-81 0.1478174117891271 1101 82-83 -0.07431786886571601 1101 84-85 -0.12506704387594425 1101 86-87 -0.06251495740140811 1101 88-89 -0.10375142999166087 1101 90-91 -0.31415596693531356 1101 92-93 -0.05008438052415798 1101 94-95 -0.31418138761668146 1101 96-97 -0.3663281792252029 1101 98-99 0.017931951460369078 1101 100-101 -0.15744803133718577 1102 1 0.3247824965357786 1102 2 0.34011892793284915 1102 3 0.4330756569303844 1102 4 0.3614836411601061 1102 5 0.47506156327339255 1102 6 0.5069453237429187 1102 7 0.5433517880439922 1102 8 0.6005673745813525 1102 9 0.5879104055607058 1102 10-11 0.6836988250158313 1102 12-13 0.660501556728903 1102 14-15 0.766579240297709 1102 16-17 0.7103921434344898 1102 18-19 0.6300933986172836 1102 20-21 0.6866070716577113 1102 22-23 0.7313870124548245 1102 24-25 0.7172328530121277 1102 26-27 0.7140757481074473 1102 28-29 0.6744562396036358 1102 30-31 0.6666989210320011 1102 32-33 0.7267918008917533 1102 34-35 0.5742306472004941 1102 36-37 0.6374805645544583 1102 38-39 0.7212086530469293 1102 40-41 0.5024205622401823 1102 42-43 0.7073478400062214 1102 44-45 0.48307894107267657 1102 46-47 0.5137226444661991 1102 48-49 0.5484996250654248 1102 50-51 0.49515979482438865 1102 52-53 0.1388187281516764 1102 54-55 0.10735871217818271 1102 56-57 0.24619840873828025 1102 58-59 0.21284303475056987 1102 60-61 0.2447972515294552 1102 62-63 0.2617016851841676 1102 64-65 0.1021967989697039 1102 66-67 0.04950636797841668 1102 68-69 -6.652500044207752 1102 70-71 -7.549605431587938 1102 72-73 -1.4165507500592476 1102 74-75 -0.045369702978977244 1102 76-77 0.6420260188690534 1102 78-79 0.2494513154618545 1102 80-81 0.32150140093331814 1102 82-83 0.4707781801756248 1102 84-85 0.18004118503240107 1102 86-87 0.42857563447429214 1102 88-89 0.3090997927499437 1102 90-91 0.10932191545033376 1102 92-93 0.2999785305006313 1102 94-95 0.6341884121653223 1102 96-97 0.5209442255121424 1102 98-99 0.23238191890757065 1102 100-101 0.20510509556020295 1103 1 0.32675231330867405 1103 2 0.15290310281331188 1103 3 0.2744541872080468 1103 4 0.15656934312875137 1103 5 0.2982646390046284 1103 6 0.28310755298647905 1103 7 0.29683444757407784 1103 8 0.31201269295858935 1103 9 0.39463467282834586 1103 10-11 0.4515875036913357 1103 12-13 0.23537700263204187 1103 14-15 0.2772312966007533 1103 16-17 0.5486334790684282 1103 18-19 0.5357780166531683 1103 20-21 0.5927884121788551 1103 22-23 0.5271241624948999 1103 24-25 0.5604227397209911 1103 26-27 0.6687454196516995 1103 28-29 0.719234357777701 1103 30-31 0.5016504705820708 1103 32-33 0.6695171907201924 1103 34-35 0.4909130531940207 1103 36-37 0.6200926098879833 1103 38-39 0.6364926598633929 1103 40-41 0.7598631297032128 1103 42-43 0.5637971759133364 1103 44-45 0.5473615532247109 1103 46-47 0.6716851966060489 1103 48-49 0.7049960188921887 1103 50-51 0.4934311365513224 1103 52-53 0.41905557567655194 1103 54-55 0.418039152623237 1103 56-57 0.47348922774882496 1103 58-59 0.7524682573686761 1103 60-61 0.7857744556487845 1103 62-63 0.4523230738089552 1103 64-65 0.37955628412832 1103 66-67 0.35894260129606437 1103 68-69 -6.7864022341616845 1103 70-71 -7.631284534990513 1103 72-73 -0.8951970185582958 1103 74-75 0.5285432430708781 1103 76-77 0.7301251670246707 1103 78-79 0.34918689477045817 1103 80-81 0.14026011758594947 1103 82-83 -0.09204307549186908 1103 84-85 -0.441664893975819 1103 86-87 -0.366256637654935 1103 88-89 -0.11411149944345667 1103 90-91 -0.467776777065108 1103 92-93 -0.3312127604763049 1103 94-95 -0.18966504456433242 1103 96-97 0.013020674890729111 1103 98-99 0.11953361293932474 1103 100-101 0.010463509377519387 1104 1 0.2579452643310276 1104 2 0.43513210946429126 1104 3 0.22495127176069118 1104 4 0.2709709367889914 1104 5 0.5645826492407906 1104 6 0.2832380050252894 1104 7 0.5344028354117967 1104 8 0.6444747312422834 1104 9 0.5585897018236494 1104 10-11 0.5756574273682489 1104 12-13 0.3833148050376778 1104 14-15 0.6741537703727971 1104 16-17 0.5863754684654765 1104 18-19 0.5960529140476183 1104 20-21 0.5757995428173999 1104 22-23 0.6795054658457147 1104 24-25 0.6505460670715166 1104 26-27 0.6782869309091666 1104 28-29 0.7595819276846001 1104 30-31 0.5851480800571167 1104 32-33 0.8349398916587276 1104 34-35 0.5793963873405232 1104 36-37 0.9465439926276886 1104 38-39 0.8486149415214612 1104 40-41 0.9031678085714674 1104 42-43 0.8630193772586239 1104 44-45 0.8747859628427292 1104 46-47 0.8246552642646421 1104 48-49 0.9678283298898478 1104 50-51 0.7968359347641609 1104 52-53 0.7000193359617555 1104 54-55 0.7544078315385505 1104 56-57 0.849578303307549 1104 58-59 0.9811877062451515 1104 60-61 0.9279692541073068 1104 62-63 0.5630894847273424 1104 64-65 0.4842781288931626 1104 66-67 0.4735421221875953 1104 68-69 -6.724337687046525 1104 70-71 -3.87982404309982 1104 72-73 0.5024858487290764 1104 74-75 1.1903636337972259 1104 76-77 1.189050249145442 1104 78-79 0.8567602065670918 1104 80-81 0.4257992149616534 1104 82-83 0.42029727214724133 1104 84-85 0.6132701204932829 1104 86-87 0.45810093795793705 1104 88-89 0.5600507960418284 1104 90-91 0.36390998855825174 1104 92-93 0.4042175288261767 1104 94-95 0.5159405176060972 1104 96-97 0.5495406712505293 1104 98-99 0.579290024034183 1104 100-101 0.44466360481166234 1105 1 0.04592341614867479 1105 2 0.13064046571184207 1105 3 0.1270756519969538 1105 4 0.1315751316591971 1105 5 0.3228056280902365 1105 6 0.08803526690626029 1105 7 0.2818583888000106 1105 8 0.3484221244722008 1105 9 0.32261685401461904 1105 10-11 0.20501565672342537 1105 12-13 0.24821428015611247 1105 14-15 0.13301290801202725 1105 16-17 0.2759630948222025 1105 18-19 0.40599083397258084 1105 20-21 0.4846794035254689 1105 22-23 0.3341590374983383 1105 24-25 0.35740223183312025 1105 26-27 0.3926712775017194 1105 28-29 0.32192339792205615 1105 30-31 0.31183274214082246 1105 32-33 0.592410395138451 1105 34-35 0.3951151692971564 1105 36-37 0.3187944150594788 1105 38-39 0.3116867413842641 1105 40-41 0.38543990023702435 1105 42-43 0.3163329942517379 1105 44-45 0.3777540410891973 1105 46-47 0.22293910620162194 1105 48-49 0.46274406464690543 1105 50-51 0.25445305952663233 1105 52-53 0.04583833255384917 1105 54-55 0.23772430416713775 1105 56-57 0.24069340324279054 1105 58-59 0.32594672386541035 1105 60-61 0.2638536903455595 1105 62-63 0.06100226546150367 1105 64-65 -0.10161050680899919 1105 66-67 0.21781533896666616 1105 68-69 -2.8330900897398053 1105 70-71 -3.2009537914508037 1105 72-73 0.30219815974034603 1105 74-75 0.6419090193748609 1105 76-77 0.6435086914220101 1105 78-79 0.39544566809160386 1105 80-81 0.15660689356999313 1105 82-83 -0.11676222551459858 1105 84-85 -0.25694056232583407 1105 86-87 -0.063677996091279 1105 88-89 -0.1349517390631192 1105 90-91 -0.06597473358636208 1105 92-93 -0.24528852851484473 1105 94-95 -0.16825071192494434 1105 96-97 -0.08155082255534651 1105 98-99 -0.1303578414897224 1105 100-101 -0.046522320367945014 1106 1 0.15451210047904596 1106 2 0.1666276110252909 1106 3 0.13651290457429255 1106 4 0.14815323529068536 1106 5 0.25681379963910445 1106 6 0.24981984461051354 1106 7 0.25669374034267634 1106 8 0.2280991068335183 1106 9 0.24863652319287866 1106 10-11 0.2940141206950173 1106 12-13 0.11780961155347569 1106 14-15 0.12977750172521496 1106 16-17 0.2166183333650764 1106 18-19 0.2671543101172773 1106 20-21 0.26889492023109085 1106 22-23 0.22285420123326105 1106 24-25 0.22588468127374028 1106 26-27 0.4443323310820375 1106 28-29 0.3981352659234503 1106 30-31 0.32716568809931346 1106 32-33 0.5043361631611489 1106 34-35 0.43546690384101794 1106 36-37 0.617778625855685 1106 38-39 0.5750429379836746 1106 40-41 0.5029781659546515 1106 42-43 0.4079764631425036 1106 44-45 0.6460132688910747 1106 46-47 0.542831370262661 1106 48-49 0.625753763394485 1106 50-51 0.7016845576518449 1106 52-53 0.3851868432379604 1106 54-55 0.39655857305259445 1106 56-57 0.47443223494427045 1106 58-59 0.4118160775686377 1106 60-61 0.6260122058961919 1106 62-63 0.42264594151063406 1106 64-65 0.48590134112181005 1106 66-67 0.5522455362731336 1106 68-69 -2.6625683528832376 1106 70-71 -2.819299465423594 1106 72-73 0.7463413000643335 1106 74-75 1.3133830055468678 1106 76-77 1.2583404839158767 1106 78-79 1.0382012208699543 1106 80-81 0.6569852123065836 1106 82-83 0.3633700977461949 1106 84-85 0.40022217619920397 1106 86-87 0.4037728386041479 1106 88-89 0.2126579759175229 1106 90-91 0.6269280884201045 1106 92-93 0.43012774944686427 1106 94-95 0.5909008488775669 1106 96-97 0.19697717795542147 1106 98-99 0.3543520026422762 1106 100-101 0.5838480482415171 1107 1 -0.026871639523672997 1107 2 0.10102999935325485 1107 3 0.12173267241049857 1107 4 -0.05504340717181577 1107 5 0.18593430881538353 1107 6 0.09392477974414959 1107 7 0.10534859950796971 1107 8 0.14248275369769914 1107 9 0.30385736461066415 1107 10-11 4.537260435810708E-4 1107 12-13 0.24943622272569854 1107 14-15 0.15524182575630618 1107 16-17 0.012104598275243461 1107 18-19 0.08400182669558376 1107 20-21 0.27324790247718056 1107 22-23 0.35833290841353715 1107 24-25 0.21557403226879046 1107 26-27 0.3190613583815889 1107 28-29 0.2765731265242195 1107 30-31 0.3503333651887246 1107 32-33 0.40477142583669234 1107 34-35 0.3239038259466298 1107 36-37 0.15099495538693475 1107 38-39 0.309054037821781 1107 40-41 0.040847511787980295 1107 42-43 -0.08986215408442888 1107 44-45 0.24288320981564482 1107 46-47 0.281764948268318 1107 48-49 0.43720315539893306 1107 50-51 0.4013257610017078 1107 52-53 0.07421398636628851 1107 54-55 -0.08899944407779259 1107 56-57 0.24739337200224298 1107 58-59 0.4388744213948712 1107 60-61 0.38436339078186776 1107 62-63 0.15457439974084508 1107 64-65 0.030872855661019827 1107 66-67 0.20610612850623156 1107 68-69 -2.7179205480731667 1107 70-71 -2.430382808769913 1107 72-73 0.8396972165297321 1107 74-75 0.9380935473358534 1107 76-77 0.5017173387768956 1107 78-79 0.4545773924780576 1107 80-81 0.06247324045006408 1107 82-83 0.10559729604666757 1107 84-85 0.09148055600108407 1107 86-87 0.27945937980159385 1107 88-89 0.16356437026892223 1107 90-91 0.3071662600040348 1107 92-93 0.04804397737889943 1107 94-95 0.41658834989960525 1107 96-97 0.3961368693042715 1107 98-99 0.667048100137702 1107 100-101 0.8785483234623293 1108 1 0.025480881013283607 1108 2 0.09401211636011908 1108 3 -0.009649335298490769 1108 4 0.027206152439134712 1108 5 0.03581905187816403 1108 6 -0.13970815379925483 1108 7 -0.03330418787859202 1108 8 0.11203995195125316 1108 9 0.23909619210024147 1108 10-11 0.19598101563063608 1108 12-13 -0.10723857829984951 1108 14-15 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0.3245427066290709 2316 100-101 0.2750740925566184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 44.0 5 182.0 6 459.0 7 1043.0 8 1825.0 9 3323.0 10 5409.0 11 7694.0 12 9862.0 13 11953.0 14 14545.0 15 18467.0 16 22211.0 17 24285.0 18 21739.0 19 17829.0 20 17716.0 21 18087.0 22 19052.0 23 20901.0 24 24690.0 25 29538.0 26 36643.0 27 45020.0 28 54797.0 29 65039.0 30 76894.0 31 89023.0 32 102409.0 33 119408.0 34 139258.0 35 158317.0 36 165774.0 37 149875.0 38 68828.0 39 3806.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.1791710178807 26.16668423120036 16.982796337635047 20.671348413283898 2 13.719213285719487 25.014961827380432 40.306811671311166 20.959013215588914 3 15.53595649408537 30.77349950317635 33.72979179385267 19.96075220888561 4 10.368166200559328 21.687127447063524 42.051298441869754 25.893407910507392 5 9.45115351187804 42.02050247867598 37.04974019082261 11.478603818623371 6 23.850080174615268 44.2587503999152 18.9608016056635 12.930367819806035 7 22.493784950672335 37.50211531989704 24.60201653046667 15.402083198963956 8 23.02049162269462 37.31666961481062 22.113250874704736 17.54958788779002 9 24.306542261377547 18.246733467138625 24.64001246522095 32.806711806262875 10-11 20.625665605954886 30.42166087346661 30.525635109825984 18.42703841075252 12-13 22.0226992184332 29.490880017778267 30.367372795038435 18.1190479687501 14-15 20.344460288500898 30.588196227610336 29.002680150221238 20.064663333667525 16-17 16.80453833426248 33.67430867799654 30.494351987197536 19.026801000543443 18-19 16.581069245626228 31.80488321978704 34.35380785329331 17.26023968129342 20-21 17.134665894401955 31.198525885448962 34.36847244209132 17.298335778057762 22-23 17.52248150328809 30.87083017764251 33.84001062611034 17.76667769295905 24-25 17.40680320851961 31.997131350248274 33.68166481309233 16.91440062813978 26-27 17.077531966389987 31.32880239989399 34.60713550173143 16.98653013198459 28-29 16.69025743364598 30.790149670724404 35.28416683296747 17.235426062662142 30-31 17.287646152966865 30.8952979766002 34.57672913020725 17.240326740225683 32-33 17.065046602805396 31.367770227143776 34.40146262978971 17.165720540261116 34-35 17.331967990436215 31.114395682502217 34.3225363958396 17.23109993122197 36-37 17.918682412976963 30.558138821404395 33.694394004181376 17.828784761437262 38-39 16.747727738356147 31.912407377990487 33.805743004067814 17.534121879585555 40-41 16.62587546107803 31.290641414146258 34.8588892712898 17.224593853485914 42-43 16.86811093826097 31.060329102694716 34.08579602177656 17.985763937267755 44-45 17.130209726431143 31.0808889110453 33.695015478065635 18.093885884457926 46-47 17.79753716068852 31.4584501720025 32.79487683406752 17.94913583324146 48-49 17.31650547979961 31.514008334893756 32.85627005515907 18.31321613014756 50-51 17.01475253523251 31.440461978542235 32.868870898061544 18.675914588163717 52-53 17.177960367203678 32.11437958820895 32.712775643970645 17.994884400616733 54-55 17.828237733263265 31.46673737307587 32.21551185743288 18.489513036227983 56-57 17.75137837279351 32.06783545567984 31.712906257874764 18.46787991365189 58-59 17.267782458847606 31.21421600312715 32.50144988260422 19.016551655421026 60-61 17.953710983279166 32.03674100491068 31.849069883143834 18.160478128666323 62-63 17.446440567996916 31.50424286236114 32.679619189692474 18.369697379949475 64-65 17.569687543206964 31.396522496039726 32.250268142613876 18.783521818139437 66-67 17.357795372179805 31.616136324317722 31.49532270953791 19.530745593964564 68-69 18.011847106720435 30.75057402512204 31.804014638444567 19.433564229712953 70-71 18.17166629679292 30.82898208275745 32.2766110202621 18.722740600187525 72-73 18.27546772117483 30.506119699835587 31.830033295704368 19.38837928328521 74-75 17.279275877480075 31.698569369890862 31.381379222879346 19.640775529749714 76-77 16.760738739822365 31.314132052366432 32.420731855563645 19.504397352247558 78-79 17.159352146615483 30.965913899073005 31.774756659005828 20.099977295305685 80-81 17.425554469924837 31.245058783710427 31.15121558710271 20.178171159262025 82-83 17.61527730636832 31.26984243553593 31.189219444924284 19.92566081317147 84-85 17.136364521679603 30.747145429286377 31.278993916486026 20.837496132547997 86-87 16.81028839933611 31.088613140270983 31.68548309331453 20.415615367078374 88-89 17.483551507743865 30.466701022830243 31.55690255557493 20.492844913850963 90-91 17.24040487898333 30.13889775847755 31.980426591736382 20.640270770802733 92-93 17.683641959047662 30.503511254117853 31.55007029532979 20.26277649150469 94-95 17.273583615479097 29.467584870596387 31.90873686887859 21.35009464504593 96-97 17.528083786623906 29.365467722410493 31.422858621251372 21.683589869714226 98-99 17.13548053341508 29.61619277539342 31.09882740649908 22.149499284692418 100-101 16.876493067186708 29.370054576685018 31.687112832370605 22.066339523757673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8429.0 1 5537.0 2 2529.5 3 2361.5 4 2035.0 5 2020.0 6 3226.0 7 5506.5 8 7473.5 9 7898.0 10 6841.5 11 5620.5 12 5066.0 13 4563.0 14 4181.5 15 4013.0 16 3941.0 17 4006.0 18 4195.5 19 4509.0 20 4867.0 21 5406.5 22 5951.0 23 6621.0 24 7494.0 25 9838.5 26 15529.0 27 21179.0 28 22648.0 29 24703.5 30 30981.0 31 38176.0 32 48340.0 33 61379.0 34 74080.0 35 84509.0 36 95021.0 37 102812.0 38 98768.5 39 89073.0 40 79531.5 41 69779.0 42 61203.0 43 57101.5 44 54755.0 45 50935.5 46 44752.5 47 38617.5 48 37440.5 49 32983.5 50 24408.5 51 17356.5 52 12271.5 53 9065.0 54 7004.5 55 5807.5 56 4913.5 57 4183.0 58 3581.5 59 3218.0 60 2715.5 61 1976.0 62 1415.0 63 1030.5 64 666.5 65 435.0 66 327.5 67 266.5 68 207.5 69 160.5 70 134.0 71 120.5 72 108.0 73 92.5 74 67.0 75 48.0 76 35.5 77 24.5 78 18.0 79 18.0 80 16.5 81 14.0 82 15.5 83 10.5 84 6.0 85 2.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01928533158956472 2 0.019540766445055643 3 5.747284248545778E-4 4 0.10102448534666023 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011781932709518843 12-13 0.0 14-15 0.0 16-17 5.108697109818469E-4 18-19 1.5964678468182713E-4 20-21 0.0 22-23 1.915761416181926E-4 24-25 0.00405502833091841 26-27 0.0034802999060638317 28-29 0.0012452449205182516 30-31 2.23505498554558E-4 32-33 6.705164956636741E-4 34-35 0.003192935693636543 36-37 0.0894021994218232 38-39 0.10226973026717848 40-41 0.0818349418279046 42-43 0.08550681787558662 44-45 0.08451700781055929 46-47 0.08298439867761374 48-49 0.07726904378600434 50-51 0.07720518507213162 52-53 0.06002719104036701 54-55 0.051055041741248315 56-57 0.027682752463828827 58-59 0.01998777744216476 60-61 0.01187772078032794 62-63 0.0021073375578001185 64-65 0.0059069310332276045 66-67 0.15271811422663584 68-69 0.711833083539331 70-71 0.8701069058728944 72-73 0.354128497781229 74-75 0.05156591145223017 76-77 0.1897242389158834 78-79 0.012867530845355269 80-81 0.0030013595520183506 82-83 0.001692255917627368 84-85 2.23505498554558E-4 86-87 0.002426631127163773 88-89 5.108697109818469E-4 90-91 0.003639946690745659 92-93 0.00405502833091841 94-95 0.074267684233986 96-97 0.03525001005774744 98-99 0.01258016663292798 100-101 0.011398780426282458 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1565957.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.944268682933036 #Duplication Level Percentage of deduplicated Percentage of total 1 72.84583544939858 15.257027500849318 2 11.197533356805108 4.6904829442206255 3 4.5317654735424515 2.847435410577371 4 2.479293331122125 2.077079426833034 5 1.5269322975555202 1.5990240150325536 6 1.0597700756713704 1.3317665524196074 7 0.7453060323958929 1.0926922855457262 8 0.6078470114443701 1.0184728900647009 9 0.45016847138804816 0.848560447560288 >10 3.2635571254293505 13.850881401043006 >50 0.570603714671293 8.525285225165687 >100 0.6347335487028758 27.62871585325528 >500 0.06194048429913346 8.79915399231471 >1k 0.023462304658762673 7.1353363606413325 >5k 0.0 0.0 >10k+ 0.0012513229151340092 3.2980856944767614 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15773 1.007243493914584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12132 0.7747339167039708 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12088 0.7719241332935707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10344 0.6605545362995281 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3783 0.24157751458054083 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3712 0.23704354589557694 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2683 0.1713329293205369 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2634 0.1682038523407731 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2582 0.1648831992193911 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2325 0.14847150975409926 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2099 0.13403944041886207 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1943 0.12407748105471607 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.1237581874853524 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1929 0.12318345906049784 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1845 0.11781932709518843 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1836 0.11724459867033386 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1792 0.1144348152599337 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1720 0.10983698786109708 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1647 0.10517530174838773 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1607 0.1026209531934785 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1587 0.10134377891602388 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1586 0.10127992020215115 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1580 0.10089676791891476 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.385871387273086E-5 0.0 8 0.0 0.0 0.0 6.385871387273086E-5 0.0 9 0.0 0.0 0.0 6.385871387273086E-5 0.0 10-11 0.0 0.0 0.0 1.2771742774546173E-4 0.0 12-13 0.0 0.0 0.0 1.2771742774546173E-4 0.0 14-15 0.0 0.0 0.0 1.915761416181926E-4 0.0 16-17 0.0 0.0 0.0 3.192935693636543E-4 6.385871387273086E-5 18-19 0.0 0.0 0.0 3.831522832363852E-4 6.385871387273086E-5 20-21 0.0 0.0 0.0 7.982339234091358E-4 6.385871387273086E-5 22-23 0.0 0.0 0.0 0.0022350549855455802 6.385871387273086E-5 24-25 0.0 0.0 0.0 0.004214675115600237 6.385871387273086E-5 26-27 0.0 0.0 0.0 0.007503398880045876 6.385871387273086E-5 28-29 0.0 0.0 0.0 0.017273782102573697 6.385871387273086E-5 30-31 0.0 0.0 0.0 0.040262919096756805 6.385871387273086E-5 32-33 0.0 0.0 0.0 0.07580029336693153 6.385871387273086E-5 34-35 0.0 0.0 0.0 0.1335605000648166 6.385871387273086E-5 36-37 0.0 0.0 0.0 0.21782207301988496 6.385871387273086E-5 38-39 0.0 0.0 0.0 0.3289362351584367 6.385871387273086E-5 40-41 0.0 0.0 0.0 0.4466917035397524 6.385871387273086E-5 42-43 0.0 0.0 0.0 0.5591149693126951 6.385871387273086E-5 44-45 0.0 0.0 0.0 0.6887800878312751 6.385871387273086E-5 46-47 0.0 0.0 0.0 0.8531524173396843 6.385871387273086E-5 48-49 0.0 0.0 0.0 1.0250600750850758 6.385871387273086E-5 50-51 0.0 0.0 0.0 1.20054382080734 6.385871387273086E-5 52-53 0.0 0.0 0.0 1.375293191320068 6.385871387273086E-5 54-55 0.0 0.0 0.0 1.5610901193327786 6.385871387273086E-5 56-57 0.0 0.0 0.0 1.7804767308425455 6.385871387273086E-5 58-59 0.0 3.192935693636543E-5 0.0 2.0018748918393032 9.57880708090963E-5 60-61 0.0 6.385871387273086E-5 0.0 2.216184735596188 1.2771742774546173E-4 62-63 0.0 6.385871387273086E-5 0.0 2.420468761275054 1.2771742774546173E-4 64-65 0.0 6.385871387273086E-5 0.0 2.6428886616937755 1.2771742774546173E-4 66-67 0.0 6.385871387273086E-5 0.0 2.8826781322858803 1.2771742774546173E-4 68-69 0.0 6.385871387273086E-5 0.0 3.1408908418302675 1.2771742774546173E-4 70-71 0.0 6.385871387273086E-5 0.0 3.481513221627414 1.2771742774546173E-4 72-73 0.0 6.385871387273086E-5 0.0 3.8295112828768607 1.2771742774546173E-4 74-75 0.0 6.385871387273086E-5 0.0 4.218123486149365 1.2771742774546173E-4 76-77 0.0 6.385871387273086E-5 0.0 4.657120214667453 1.2771742774546173E-4 78-79 0.0 6.385871387273086E-5 0.0 5.166297669731672 1.2771742774546173E-4 80-81 0.0 6.385871387273086E-5 0.0 5.710022689001039 1.2771742774546173E-4 82-83 0.0 6.385871387273086E-5 0.0 6.255791187114333 1.2771742774546173E-4 84-85 0.0 6.385871387273086E-5 0.0 6.815640531636564 1.2771742774546173E-4 86-87 0.0 6.385871387273086E-5 0.0 7.397648849872634 1.2771742774546173E-4 88-89 0.0 6.385871387273086E-5 0.0 8.00076247304364 1.2771742774546173E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 45 1.5211541E-5 52.748837 4 CATTCCG 535 0.0 39.88545 9 CCTTTAG 565 0.0 36.96496 1 GGCATTC 625 0.0 34.14194 7 TAGGCAT 680 0.0 32.775146 5 TTAGGCA 690 0.0 32.337334 4 ACCGGCG 30 0.0037540197 32.00082 66-67 ATCACGC 75 3.095833E-4 31.63716 3 CTTTAGG 740 0.0 29.504232 2 GTACATG 7470 0.0 28.276482 1 CATGGGG 1710 0.0 28.04017 4 GTGGTAC 425 0.0 27.92139 1 TATCACG 85 6.4372236E-4 27.919605 2 CGCATAG 140 0.0 27.429276 66-67 ATCGACC 35 0.007974728 27.429276 66-67 GGTCAAC 245 0.0 27.096779 7 CATGGGT 710 0.0 26.74589 4 CCGCAAT 45 8.4307697E-4 26.660883 64-65 CACGCAG 90 9.03821E-4 26.34409 5 GGTACTC 415 0.0 26.300772 3 >>END_MODULE