FastQCFastQC Report
Fri 27 May 2016
SRR939413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929246
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153550.5241963290211884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112870.38532100069437664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84270.28768495373894853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56230.19196066154908123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85850.049.253321
GTATCAA154300.041.5926481
TATCAAC197300.032.5728342
ATCAACG203700.031.2399863
TCAACGC209050.030.6233484
CAACGCA209850.030.1936725
AACGCAG214450.029.5523686
GTACATG140200.026.479121
ACGCAGA235900.026.3242427
CGCAGAG238000.026.1228568
GCAGAGT237300.024.78099
TACATGG145000.024.484612
GAGTACT143550.024.05605112-13
ACATGGG145350.023.1762983
GTACTTT155800.022.34147614-15
AGTACTT149150.021.99680512-13
CAGAGTA230400.021.31573710-11
AGAGTAC227150.020.45378110-11
CATGGGG63350.020.2562774
GTGGTAT43300.020.1323591
Produced by FastQC (version 0.11.5)