FastQCFastQC Report
Fri 27 May 2016
SRR939413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2929246
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211090.7206291311825638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173680.592917085147509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131230.4479992462224068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82790.2826324590013949No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.1432450535052365No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41120.14037742135689527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94850.048.1771051
GTATCAA162450.039.01331
TATCAAC217650.029.0010072
ATCAACG219500.028.7286133
TCAACGC222150.028.3859124
CAACGCA227750.027.7087525
AACGCAG232750.027.1135066
ACGCAGA268400.023.441577
GTACATG161200.023.4365831
CGCAGAG270750.023.1506148
TACATGG162950.022.5961342
GCAGAGT283700.021.3757699
ACATGGG163550.021.3495183
GAGTACT166650.021.2512512-13
CTAACGG1353.864742E-421.0566189
AGTACTT173250.020.76983612-13
GTGGTAT47300.020.7448461
GTACTTT172050.020.54294414-15
TACTTTT182950.019.56503314-15
CATGGGA98000.019.2893664
Produced by FastQC (version 0.11.5)