##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939413_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2929246 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.993279499229494 31.0 31.0 31.0 30.0 34.0 2 31.248783133953243 31.0 31.0 34.0 30.0 34.0 3 31.235019182410763 31.0 31.0 34.0 28.0 34.0 4 35.18124356916422 35.0 35.0 37.0 33.0 37.0 5 34.794099232362186 35.0 35.0 37.0 32.0 37.0 6 34.74621387210224 35.0 35.0 37.0 32.0 37.0 7 34.61052332238398 35.0 35.0 37.0 30.0 37.0 8 34.631560135270306 35.0 35.0 37.0 30.0 37.0 9 35.98266243258504 37.0 35.0 39.0 30.0 39.0 10-11 35.837391772490264 37.0 35.0 39.0 30.0 39.0 12-13 35.78864031904456 37.0 35.0 39.0 30.0 39.0 14-15 36.6427160777893 38.0 35.5 40.0 30.5 41.0 16-17 36.653425659709015 38.0 35.5 40.0 30.5 41.0 18-19 36.61653288935105 38.0 35.0 40.0 30.0 41.0 20-21 36.52278470295769 38.0 35.0 40.0 30.0 41.0 22-23 36.379277636634136 38.0 35.0 40.0 30.0 41.0 24-25 36.14114178187834 38.0 34.0 40.0 29.5 41.0 26-27 35.757050449159955 38.0 34.0 40.0 27.5 41.0 28-29 35.460438966204954 38.0 34.0 40.0 27.0 41.0 30-31 35.20083137435367 37.5 34.0 40.0 27.0 41.0 32-33 34.96906678373889 37.0 33.0 40.0 27.0 41.0 34-35 34.86713270240874 37.0 33.0 40.0 26.0 41.0 36-37 34.82719648674096 38.0 33.0 40.0 25.5 41.0 38-39 34.787101527150675 38.0 33.0 40.0 25.0 41.0 40-41 34.53967573908098 37.5 33.0 40.0 24.5 41.0 42-43 34.198928495592384 37.0 33.0 40.0 24.0 41.0 44-45 33.98736825107895 37.0 33.0 40.0 24.0 41.0 46-47 33.612366800193634 37.0 32.5 40.0 22.5 41.0 48-49 33.269103892264425 37.0 32.0 40.0 21.0 41.0 50-51 32.979917016187784 36.5 31.0 40.0 20.0 41.0 52-53 32.585279283474314 36.0 31.0 39.0 18.5 41.0 54-55 32.34057620971403 36.0 31.0 39.0 18.0 40.0 56-57 31.9323447057707 35.0 30.0 39.0 15.0 40.0 58-59 31.486650660272304 35.0 29.5 39.0 12.5 40.0 60-61 31.185059738922575 35.0 29.0 38.0 10.5 40.0 62-63 30.777901378033803 34.5 29.0 38.0 8.0 40.0 64-65 30.324703183003408 34.0 28.0 38.0 7.5 40.0 66-67 28.669109047174597 33.5 25.0 36.5 4.5 39.5 68-69 25.08719496416484 29.5 15.5 35.0 2.0 38.0 70-71 24.503868230937243 29.5 13.5 34.5 2.0 37.5 72-73 26.280977254897678 31.0 21.0 35.0 2.0 37.0 74-75 26.868514798688807 31.5 23.0 35.0 2.0 37.0 76-77 26.884774955739463 31.5 25.0 34.0 2.0 36.0 78-79 27.713469780277926 32.5 26.0 35.0 2.0 36.0 80-81 27.588142477620522 33.0 26.0 35.0 2.0 36.0 82-83 27.202922014743727 32.5 25.5 35.0 2.0 36.0 84-85 26.886846137197082 32.0 25.0 35.0 2.0 35.5 86-87 26.44922754865928 32.0 24.5 34.5 2.0 35.0 88-89 25.9833296691367 32.0 23.5 34.0 2.0 35.0 90-91 25.55249832209381 32.0 22.0 34.0 2.0 35.0 92-93 25.013318956482316 31.0 19.5 34.0 2.0 35.0 94-95 23.6675536981189 31.0 7.0 34.0 2.0 35.0 96-97 22.88119519494095 30.0 2.0 34.0 2.0 35.0 98-99 22.546225376769314 30.0 2.0 34.0 2.0 35.0 100-101 21.32178553798486 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36344081055380073 1101 2 0.27685439913035736 1101 3 0.36012662860360223 1101 4 0.28077436035416525 1101 5 0.37964796150447455 1101 6 0.49947180787911805 1101 7 0.519199789954726 1101 8 0.4927681369662693 1101 9 0.7591662212336985 1101 10-11 0.8509371534380747 1101 12-13 0.6371870994245157 1101 14-15 0.8091210804933198 1101 16-17 0.73335731664924 1101 18-19 0.8048327992856485 1101 20-21 0.8798803414510914 1101 22-23 1.0072324487400692 1101 24-25 0.9212746606205258 1101 26-27 0.8057346714094038 1101 28-29 0.8403584658888832 1101 30-31 0.9523414009458762 1101 32-33 0.8341933655984519 1101 34-35 0.8520190710511244 1101 36-37 0.9344022013856872 1101 38-39 0.8711106652042346 1101 40-41 1.0167560034915866 1101 42-43 1.1083980369617237 1101 44-45 0.8479831272121388 1101 46-47 0.9394976961063009 1101 48-49 0.9327628139322002 1101 50-51 0.8269876985970441 1101 52-53 0.90726689500611 1101 54-55 0.7336753093664115 1101 56-57 0.6880307020323606 1101 58-59 0.6543057759705846 1101 60-61 0.46970066467877203 1101 62-63 0.47193503611431353 1101 64-65 0.1915476874943849 1101 66-67 -2.814719716195249 1101 68-69 -7.6519360859215055 1101 70-71 -11.177351843917265 1101 72-73 -11.142337208674144 1101 74-75 -9.458555474566626 1101 76-77 -4.576370310685274 1101 78-79 -1.5317442696562829 1101 80-81 -0.8829314901340375 1101 82-83 -0.6679745941405493 1101 84-85 -0.670147551110162 1101 86-87 -0.6005459317422783 1101 88-89 -0.6274067209291054 1101 90-91 -0.7722369487223304 1101 92-93 -1.0667938769754208 1101 94-95 -4.741865549839336 1101 96-97 -1.5556012497880047 1101 98-99 -0.8480210201864296 1101 100-101 -0.6396826900899306 1102 1 0.36627443336361765 1102 2 0.2043799600168228 1102 3 0.35168424094356254 1102 4 0.17866853371351965 1102 5 0.38171634448524827 1102 6 0.38055974579130236 1102 7 0.35745026488643106 1102 8 0.47436954800943454 1102 9 0.6647231717230397 1102 10-11 0.6634124787448386 1102 12-13 0.6178906909421329 1102 14-15 0.47467105720080127 1102 16-17 0.6138431820668302 1102 18-19 0.5853566672236994 1102 20-21 0.7182256230908806 1102 22-23 0.8239735769279264 1102 24-25 0.6728104294949375 1102 26-27 0.702990296640678 1102 28-29 0.5305454127416382 1102 30-31 0.4945904635570173 1102 32-33 0.4593280909204651 1102 34-35 0.5533384710583888 1102 36-37 0.7752702895275831 1102 38-39 0.6457611078817038 1102 40-41 0.7190632893970914 1102 42-43 0.6667965740243673 1102 44-45 0.5661079143960279 1102 46-47 0.49875153937064454 1102 48-49 0.4400874305039295 1102 50-51 0.5485899958334386 1102 52-53 0.5481959688104396 1102 54-55 0.41951177597828604 1102 56-57 0.2953565361218189 1102 58-59 0.2755148549004609 1102 60-61 0.035295779358957446 1102 62-63 0.16565428131719173 1102 64-65 0.10935486222654234 1102 66-67 -3.0387940733957883 1102 68-69 -7.777887097000118 1102 70-71 -11.067353608320001 1102 72-73 -9.46131767130693 1102 74-75 -7.927871582884986 1102 76-77 -4.286300554903367 1102 78-79 -1.4666706916670869 1102 80-81 -0.761205115865895 1102 82-83 -0.8450871969659062 1102 84-85 -0.7353412342290184 1102 86-87 -0.6715307079804838 1102 88-89 -0.5436850127477477 1102 90-91 -0.6161395500986444 1102 92-93 -1.040027298675259 1102 94-95 -4.760140790279124 1102 96-97 -1.7226551103770333 1102 98-99 -0.8603451437632792 1102 100-101 -0.5766838321614749 1103 1 0.32295745719576985 1103 2 0.2903373478117075 1103 3 0.3037554020568116 1103 4 0.325940905199559 1103 5 0.4224554608849118 1103 6 0.47934463274194883 1103 7 0.4301132769815439 1103 8 0.4334112435028956 1103 9 0.6170996095695997 1103 10-11 0.5795932033544702 1103 12-13 0.5648438881111346 1103 14-15 0.8063095186609175 1103 16-17 0.6120715584451233 1103 18-19 0.5062539516417104 1103 20-21 0.7185249255281576 1103 22-23 0.6894232239939342 1103 24-25 0.9142261496111246 1103 26-27 0.8901550504879268 1103 28-29 0.9554146735978222 1103 30-31 0.9331278853720946 1103 32-33 0.8331181490650579 1103 34-35 0.831104305685173 1103 36-37 0.9062026675481221 1103 38-39 0.8011227741344129 1103 40-41 0.8629543562378998 1103 42-43 0.8020901391786666 1103 44-45 0.6874756893174663 1103 46-47 0.5391063910083105 1103 48-49 0.6380795675468462 1103 50-51 0.5479211684956908 1103 52-53 0.5296141452437411 1103 54-55 0.4819326900103178 1103 56-57 0.5496989189139647 1103 58-59 0.49049779191923903 1103 60-61 0.40636150954329864 1103 62-63 0.5233236810120907 1103 64-65 0.4272506799059137 1103 66-67 -2.9593753567294243 1103 68-69 -7.726451297372915 1103 70-71 -11.497982041581434 1103 72-73 -9.274695305187965 1103 74-75 -7.581688942583053 1103 76-77 -3.8286909275505714 1103 78-79 -0.9393868162333163 1103 80-81 -0.2664151917022366 1103 82-83 -0.0190329883583793 1103 84-85 -0.20220955134229968 1103 86-87 0.14567747801952535 1103 88-89 0.1903454491103922 1103 90-91 0.17975102547874755 1103 92-93 0.10981749694896692 1103 94-95 1.0296748566568255 1103 96-97 1.1143145288381646 1103 98-99 0.7132041698390772 1103 100-101 0.6557105050677556 1104 1 0.36169070858150576 1104 2 0.23345826542069759 1104 3 0.3400269726187446 1104 4 0.16954734701548801 1104 5 0.4472959539175747 1104 6 0.4675603284112313 1104 7 0.5041537093954389 1104 8 0.4375625194820927 1104 9 0.6371336540509134 1104 10-11 0.6854045304922352 1104 12-13 0.6396840558536852 1104 14-15 0.7482685764982691 1104 16-17 0.828350262428053 1104 18-19 0.8152881767547271 1104 20-21 0.8089448635929202 1104 22-23 0.6253996224735658 1104 24-25 0.8034332912983189 1104 26-27 0.906852096700554 1104 28-29 0.8480307600744723 1104 30-31 0.8973976358401288 1104 32-33 0.7502462812262252 1104 34-35 0.7988241405755616 1104 36-37 0.9614719465368395 1104 38-39 0.8688795581555482 1104 40-41 0.9872638801203237 1104 42-43 1.0142130705248462 1104 44-45 0.7851611735711259 1104 46-47 0.7823662840376073 1104 48-49 0.7801174029974973 1104 50-51 0.7750680067522495 1104 52-53 0.6185197621239311 1104 54-55 0.7155218034301853 1104 56-57 0.7413693296061403 1104 58-59 0.9175994148490503 1104 60-61 0.5618453077991283 1104 62-63 0.6117345698883945 1104 64-65 0.4059457077890194 1104 66-67 -2.6001085874496717 1104 68-69 -7.077154517209337 1104 70-71 -8.48642811919489 1104 72-73 -7.984869366017982 1104 74-75 -6.786571202867197 1104 76-77 -3.4733709798419135 1104 78-79 -0.3556836407894295 1104 80-81 0.3794661378694677 1104 82-83 0.6110797112740798 1104 84-85 0.47507815667116304 1104 86-87 0.7516969908344286 1104 88-89 0.6695744311589671 1104 90-91 0.5916964249579735 1104 92-93 0.7009462804557636 1104 94-95 1.5137421465538488 1104 96-97 1.5660677851836198 1104 98-99 1.2094148269557365 1104 100-101 1.0490748544951671 1105 1 0.5338143024612165 1105 2 0.3852887922255199 1105 3 0.5021003764770526 1105 4 0.33519250584900817 1105 5 0.519961051670073 1105 6 0.5716753008790718 1105 7 0.6347536286649031 1105 8 0.49591698746025514 1105 9 0.6516881890506525 1105 10-11 0.8321419886989574 1105 12-13 0.7607476806547098 1105 14-15 1.0440032814890898 1105 16-17 1.0173494893951016 1105 18-19 0.9897454352676078 1105 20-21 1.032555498698514 1105 22-23 1.0921661550970754 1105 24-25 1.1274112122585578 1105 26-27 1.2388366208092876 1105 28-29 1.2975577057946097 1105 30-31 1.0842870159667015 1105 32-33 1.1699369157225306 1105 34-35 1.2769644441528314 1105 36-37 1.143375644909277 1105 38-39 1.071847738138942 1105 40-41 1.2060302071094569 1105 42-43 1.2613363788012464 1105 44-45 1.2042476618690046 1105 46-47 1.1399530503227169 1105 48-49 1.107246004455213 1105 50-51 1.1105165181878078 1105 52-53 1.3188924744414265 1105 54-55 1.3298533774471366 1105 56-57 1.413181901482968 1105 58-59 1.5390851613399406 1105 60-61 1.630319938609258 1105 62-63 1.5106873577574866 1105 64-65 1.4906057636911072 1105 66-67 2.0754134953981414 1105 68-69 -4.518386781243883 1105 70-71 -5.0318070576645155 1105 72-73 -1.3603974465352664 1105 74-75 1.279702473647074 1105 76-77 1.3467747159258288 1105 78-79 1.2918863510613576 1105 80-81 1.358741809798854 1105 82-83 1.5502778133448984 1105 84-85 1.3863752907371634 1105 86-87 1.5105684960019445 1105 88-89 1.5227111170943353 1105 90-91 1.440200815632835 1105 92-93 1.5280622201668095 1105 94-95 2.3948932802085814 1105 96-97 2.380867082218675 1105 98-99 2.050763141134606 1105 100-101 1.9542289470001748 1106 1 0.18504314416625078 1106 2 0.23120830481169818 1106 3 0.16886782419688373 1106 4 0.0652682061628056 1106 5 0.2595221959931209 1106 6 0.2604886039780894 1106 7 0.3373556122192696 1106 8 0.23201950927705894 1106 9 0.5281747095847891 1106 10-11 0.46793930425817365 1106 12-13 0.42956796971309785 1106 14-15 0.49617265092036433 1106 16-17 0.37053640389153486 1106 18-19 0.3667619152602768 1106 20-21 0.5119423376091632 1106 22-23 0.5684405200619196 1106 24-25 0.5171790722645042 1106 26-27 0.6212984270806103 1106 28-29 0.7444034306165292 1106 30-31 0.5640910082707009 1106 32-33 0.6524388053672823 1106 34-35 0.8656758209594457 1106 36-37 0.91879165306338 1106 38-39 0.7697662658193778 1106 40-41 0.7900503976772626 1106 42-43 0.8709595378291723 1106 44-45 0.7113704773251541 1106 46-47 0.6114709504120412 1106 48-49 0.3701256512634288 1106 50-51 0.36100267224539095 1106 52-53 0.6221778795125417 1106 54-55 0.5660898922550786 1106 56-57 0.4952935508565268 1106 58-59 0.6729968404897164 1106 60-61 0.6751511876071667 1106 62-63 0.6267092547317681 1106 64-65 0.7082630623007873 1106 66-67 1.294686153956512 1106 68-69 -5.3699405845834 1106 70-71 -6.462343716772601 1106 72-73 -2.4404488105152033 1106 74-75 0.13578712973172813 1106 76-77 0.5047020619484108 1106 78-79 0.48198089056065996 1106 80-81 0.5527539264300181 1106 82-83 0.6801914623061656 1106 84-85 0.7066523241094345 1106 86-87 0.8000812539670825 1106 88-89 0.8297944217966879 1106 90-91 0.8078735749722625 1106 92-93 0.9778240104047917 1106 94-95 1.9259928244033624 1106 96-97 2.055646909974694 1106 98-99 1.7021093698617875 1106 100-101 1.6784184039277257 1107 1 -0.05119031798551532 1107 2 -0.013078778811433267 1107 3 -0.0046686746494444264 1107 4 0.019817534364314326 1107 5 0.07575052942426197 1107 6 0.08489403166748133 1107 7 0.07180845152200277 1107 8 -0.002876669961651146 1107 9 0.13583048721059043 1107 10-11 0.07947575894482384 1107 12-13 0.18596671689525834 1107 14-15 0.15561930689961656 1107 16-17 -0.04167521936209795 1107 18-19 0.21934930445677026 1107 20-21 0.158212704082203 1107 22-23 0.19811411030774195 1107 24-25 0.3882649122890953 1107 26-27 0.42491709004561784 1107 28-29 0.3292236668281987 1107 30-31 0.17488870832615078 1107 32-33 0.2619257570536888 1107 34-35 0.3220866830009328 1107 36-37 0.46272956532933307 1107 38-39 0.35630960108760235 1107 40-41 0.3798949594805734 1107 42-43 0.5054120091345808 1107 44-45 0.5023034977445491 1107 46-47 0.25300126922749655 1107 48-49 0.4421807865595824 1107 50-51 0.42466175487454905 1107 52-53 0.6749199825320389 1107 54-55 0.5510652877602027 1107 56-57 0.659700665523129 1107 58-59 0.519083460087586 1107 60-61 0.49161862245856724 1107 62-63 0.6395680497032181 1107 64-65 0.6155652622856422 1107 66-67 1.3902430995089823 1107 68-69 -5.1565522995652096 1107 70-71 -5.59836990927278 1107 72-73 -2.029551179817563 1107 74-75 0.4395654841677725 1107 76-77 0.49951199229814236 1107 78-79 0.5232252076651172 1107 80-81 0.44771005824355825 1107 82-83 0.561688963507482 1107 84-85 0.5929192159129926 1107 86-87 0.5960346294806556 1107 88-89 0.44658355409967143 1107 90-91 0.3180598862077453 1107 92-93 0.40458339109240526 1107 94-95 1.2638288955399624 1107 96-97 1.4859763973486082 1107 98-99 1.1834139436230728 1107 100-101 1.0743275519732443 1108 1 0.0796663706328502 1108 2 0.06337405312262945 1108 3 0.1437535231573186 1108 4 -0.014471039632226734 1108 5 0.04272870877230872 1108 6 0.20117977406314225 1108 7 0.172897759445668 1108 8 0.09700399436703577 1108 9 0.18675942006472468 1108 10-11 0.2595207337351866 1108 12-13 0.25863315478132165 1108 14-15 0.26536814343071313 1108 16-17 0.13677404014526928 1108 18-19 0.27854064996499517 1108 20-21 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2316 100-101 0.1910608500414277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 51.0 4 124.0 5 321.0 6 743.0 7 1754.0 8 3384.0 9 5612.0 10 8997.0 11 13050.0 12 16655.0 13 20671.0 14 24785.0 15 30804.0 16 36523.0 17 41380.0 18 37822.0 19 32223.0 20 31028.0 21 32094.0 22 33848.0 23 38326.0 24 45857.0 25 56530.0 26 70521.0 27 87739.0 28 107078.0 29 126922.0 30 148676.0 31 172934.0 32 199810.0 33 231147.0 34 271015.0 35 309949.0 36 322466.0 37 261531.0 38 101451.0 39 5412.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.0823490605274 20.01800886306503 15.285406674547678 22.61423540185989 2 16.256004403342935 21.451887605491066 37.7008239878389 24.59128400332709 3 18.980379251179315 25.30101602938094 30.276699191532565 25.44190552790718 4 13.577316483490973 17.57950680823666 35.49374139283625 33.34943531543612 5 14.005241634431332 37.744342472567105 33.006934565598776 15.243481327402783 6 32.18961466534391 36.05050582982788 16.58583813035846 15.174041374469743 7 28.413216233802146 33.587721891572095 19.950116856010045 18.04894501861571 8 27.417157862466997 33.93723162889016 19.1916964297297 19.45391407891314 9 26.994386951454402 16.32754640614001 19.8171474843697 36.86091915803589 10-11 25.523189243921472 26.84716476526724 26.038970438126398 21.59067555268489 12-13 26.404900783341517 24.194263643272024 27.31020201102946 22.090633562357002 14-15 23.23976886884884 25.342016341406627 25.930905086155278 25.48730970358925 16-17 22.415205734462592 28.75895668394193 26.367736717786915 22.45810086380856 18-19 22.116710449728 26.39423872948896 29.892701628251938 21.596349192531108 20-21 22.755696904099334 25.601191706598748 30.07236166185513 21.570749727446785 22-23 22.588184809333185 25.53302112557293 30.072124362378577 21.806669702715308 24-25 22.500278228595345 25.8625427840475 29.873933428602445 21.76324555875471 26-27 22.1966049827361 26.247544579914067 29.767952987026643 21.78789745032319 28-29 21.992897933401053 26.18951933008391 30.1640304227949 21.653552313720134 30-31 21.948420038496177 26.13605715296256 29.89889120357568 22.016631604965582 32-33 21.927070512638867 26.241788638592894 29.606189235242763 22.224951613525477 34-35 22.63250445162516 26.183015865726933 29.430965284408106 21.7535143982398 36-37 22.509223957325027 25.967005460815116 29.292482788752622 22.23128779310723 38-39 22.217075136608987 26.207668899355074 29.62337629716383 21.951879666872113 40-41 22.244137044486294 26.012540838217173 29.794189228195506 21.94913288910103 42-43 22.29263196458389 26.328668006777562 29.101498021616294 22.277202007022254 44-45 22.231445118942332 26.55965296696297 28.933424962375593 22.275476951719103 46-47 22.349221710932238 26.663280089575135 28.501668598314193 22.485829601178434 48-49 22.527139706544475 26.849893966632166 28.13994524984011 22.48302107698325 50-51 22.362654064423378 27.023189906950922 28.025632793353545 22.588523235272156 52-53 22.93140399781479 26.757367484025174 27.82098355659767 22.490244961562365 54-55 22.276420542374158 26.779113282991414 27.97116128602491 22.973304888609526 56-57 22.490073951028535 27.3142396199107 27.665279899895978 22.53040652916479 58-59 22.53761527343821 26.745896011229185 27.806177758308277 22.910310957024336 60-61 22.54165244110618 26.645390918402185 28.21377603277569 22.599180607715944 62-63 22.535591366408386 26.860256252034482 28.029061993833444 22.57509038772369 64-65 22.441619281738316 26.634765091895552 27.994767017392807 22.92884860897333 66-67 21.85991447723404 26.944401974825837 28.530505108123837 22.665178439816284 68-69 21.825521568619354 26.469272991337316 29.094369366230445 22.610836073812887 70-71 21.31212414168866 26.656566286243493 29.2678268901792 22.763482681888643 72-73 22.178231548678625 26.854802820758593 27.9424038066548 23.024561823907984 74-75 21.952324801817735 27.053370662631508 27.84958695147522 23.14471758407554 76-77 22.29514292147867 27.007982884624347 27.734365463870624 22.962508730026357 78-79 22.051755562486207 27.08217786948085 27.723809187275535 23.14225738075741 80-81 21.88380677773882 27.38174529830087 27.60320455331426 23.131243370646047 82-83 22.18388281489503 27.21741362794385 27.27524420960206 23.323459347559066 84-85 21.913441532981786 27.10313969928434 27.194494281889313 23.78892448584456 86-87 22.261035834703282 26.898208973210302 27.43824638562577 23.40250880646065 88-89 21.99274757470145 26.875179014804928 27.280302960473872 23.85177045001975 90-91 21.746084499666203 27.13618019093873 27.538787150375388 23.578948159019678 92-93 21.94510403965867 26.857664502079153 27.457470831154833 23.73976062710734 94-95 21.361529051537868 26.686348494601898 27.79746283697846 24.15465961688178 96-97 21.48289734437699 26.391639385993244 27.872203274775686 24.253259994854083 98-99 21.60655806550793 26.471383066024345 27.309185870297625 24.6128729981701 100-101 21.469433267077132 26.337557023919832 27.639812306719552 24.553197402283484 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6204.0 1 4162.5 2 2266.5 3 2550.0 4 2451.5 5 2478.5 6 3915.5 7 7177.5 8 10342.0 9 11517.5 10 10233.5 11 8253.5 12 7360.5 13 6957.0 14 6767.0 15 6576.5 16 6451.5 17 6397.0 18 6472.5 19 6706.5 20 7144.5 21 7815.5 22 8656.5 23 9769.0 24 11368.5 25 13628.0 26 16117.0 27 18417.5 28 21064.5 29 24276.0 30 28033.5 31 32664.0 32 38196.5 33 42387.0 34 45694.5 35 49390.0 36 54371.5 37 60309.5 38 64101.5 39 66176.5 40 68616.5 41 74542.5 42 81198.0 43 86471.5 44 90743.0 45 93672.5 46 96057.5 47 100404.5 48 110640.0 49 116340.5 50 115494.0 51 113605.5 52 110650.0 53 110650.5 54 109530.0 55 103539.5 56 95784.5 57 86669.0 58 77775.5 59 69495.0 60 60173.5 61 49970.5 62 40200.5 63 31319.0 64 23671.0 65 18195.5 66 13759.0 67 10481.5 68 7431.0 69 4901.5 70 3627.0 71 2871.0 72 2359.5 73 1949.5 74 1539.0 75 1205.0 76 832.5 77 459.5 78 259.5 79 164.5 80 102.5 81 69.0 82 47.5 83 27.0 84 15.0 85 7.0 86 4.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023487272834033058 2 0.01918582461152119 3 0.0 4 0.0 5 2.3896934569510376E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.072463016079906E-4 18-19 0.0010582928166497454 20-21 5.1207716934665104E-5 22-23 0.0 24-25 0.0 26-27 4.0966173547732083E-4 28-29 0.01778614701530701 30-31 0.004233171266598982 32-33 0.007681157540199765 34-35 0.008858935029697063 36-37 0.10359321135882749 38-39 0.10591462785986565 40-41 0.12032106555748476 42-43 0.1065120512241034 44-45 0.1008450638833338 46-47 0.10198870289487465 48-49 0.10393459613839193 50-51 0.10222767224056976 52-53 0.07841608386595049 54-55 0.0659214009338922 56-57 0.03727921792843619 58-59 0.015328176602443086 60-61 0.008398065577285075 62-63 0.0020312394384083823 64-65 0.00452334832922875 66-67 0.41403146065574553 68-69 0.7946242821531547 70-71 0.8782123454295064 72-73 0.2746781936375436 74-75 0.14635165499927286 76-77 0.01425281454681512 78-79 0.007766503735090874 80-81 0.0013314006403012926 82-83 0.0 84-85 1.7069238978221698E-5 86-87 1.536231508039953E-4 88-89 6.486310811724246E-4 90-91 0.0029529783432323538 92-93 0.0017239931368003916 94-95 0.21898126685160618 96-97 0.2233339227910527 98-99 0.013467629553816921 100-101 0.007237357326766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2929246.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.95768100276297 #Duplication Level Percentage of deduplicated Percentage of total 1 63.69095162045652 21.627970178898718 2 14.60285105738292 9.917579158749385 3 6.887586551149533 7.016594009485686 4 3.931693749267677 5.340448085527556 5 2.565257984169016 4.355510615810111 6 1.7044550027442689 3.4727603560052023 7 1.2026982252850424 2.8588589873773036 8 0.9233921038443995 2.508500360225464 9 0.6841863488631506 2.091004360302597 >10 3.4466677639671754 20.159549753313282 >50 0.20340375459991228 4.801083476891182 >100 0.14163944748242957 9.331298976067327 >500 0.011642070804246382 2.688920758220354 >1k 0.0031658262713229936 1.7533921748838874 >5k 1.0212342810719334E-4 0.2871053542426713 >10k+ 3.0637028432158004E-4 1.7894233939994972 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21109 0.7206291311825638 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17368 0.592917085147509 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13123 0.4479992462224068 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8279 0.2826324590013949 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4196 0.1432450535052365 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4112 0.14037742135689527 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.120771693466509E-5 0.0 14-15 0.0 0.0 0.0 6.827695591288679E-5 0.0 16-17 0.0 0.0 0.0 1.194846728475519E-4 0.0 18-19 0.0 0.0 0.0 1.7069238978221699E-4 0.0 20-21 0.0 0.0 0.0 3.072463016079906E-4 0.0 22-23 0.0 0.0 0.0 0.0010070850997150802 0.0 24-25 0.0 0.0 0.0 0.002065377916364826 0.0 26-27 0.0 0.0 0.0 0.0030041860601670188 0.0 28-29 0.0 0.0 0.0 0.00602544135931226 0.0 30-31 0.0 0.0 0.0 0.014645407043314218 0.0 32-33 0.0 0.0 0.0 0.027174228453328945 0.0 34-35 0.0 0.0 0.0 0.04478968307885374 0.0 36-37 0.0 1.7069238978221698E-5 0.0 0.07291978891496309 0.0 38-39 0.0 3.4138477956443396E-5 0.0 0.11304956975276231 0.0 40-41 0.0 3.4138477956443396E-5 0.0 0.16016066933265422 0.0 42-43 0.0 3.4138477956443396E-5 0.0 0.20761315369211053 0.0 44-45 0.0 3.4138477956443396E-5 0.0 0.2655632200231732 0.0 46-47 0.0 3.4138477956443396E-5 0.0 0.33556416907286035 0.0 48-49 0.0 3.4138477956443396E-5 0.0 0.41194901350040247 0.0 50-51 0.0 3.4138477956443396E-5 0.0 0.4913039055101551 0.0 52-53 0.0 3.4138477956443396E-5 0.0 0.5697711970930404 0.0 54-55 0.0 3.4138477956443396E-5 0.0 0.6562439617567115 0.0 56-57 0.0 3.4138477956443396E-5 0.0 0.7570207486841324 0.0 58-59 0.0 3.4138477956443396E-5 0.0 0.8539057491245188 0.0 60-61 0.0 3.4138477956443396E-5 0.0 0.9558944520193934 1.7069238978221698E-5 62-63 1.7069238978221698E-5 5.120771693466509E-5 0.0 1.056107954060533 3.4138477956443396E-5 64-65 3.4138477956443396E-5 6.827695591288679E-5 0.0 1.1619543049644858 3.4138477956443396E-5 66-67 3.4138477956443396E-5 6.827695591288679E-5 0.0 1.2745771437427926 3.4138477956443396E-5 68-69 3.4138477956443396E-5 6.827695591288679E-5 0.0 1.4106701861161541 3.4138477956443396E-5 70-71 3.4138477956443396E-5 6.827695591288679E-5 0.0 1.5897094337587214 3.4138477956443396E-5 72-73 3.4138477956443396E-5 6.827695591288679E-5 0.0 1.7815506106349552 3.4138477956443396E-5 74-75 3.4138477956443396E-5 6.827695591288679E-5 0.0 1.9978690762059588 3.4138477956443396E-5 76-77 3.4138477956443396E-5 6.827695591288679E-5 0.0 2.232673527590377 3.4138477956443396E-5 78-79 3.4138477956443396E-5 6.827695591288679E-5 0.0 2.4979124320729635 3.4138477956443396E-5 80-81 3.4138477956443396E-5 6.827695591288679E-5 0.0 2.793244404874155 3.4138477956443396E-5 82-83 3.4138477956443396E-5 6.827695591288679E-5 0.0 3.0929290336147934 3.4138477956443396E-5 84-85 3.4138477956443396E-5 6.827695591288679E-5 0.0 3.40338435215069 3.4138477956443396E-5 86-87 3.4138477956443396E-5 6.827695591288679E-5 0.0 3.7237568985329332 3.4138477956443396E-5 88-89 3.4138477956443396E-5 6.827695591288679E-5 0.0 4.068111725679578 3.4138477956443396E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9485 0.0 48.177105 1 GTATCAA 16245 0.0 39.0133 1 TATCAAC 21765 0.0 29.001007 2 ATCAACG 21950 0.0 28.728613 3 TCAACGC 22215 0.0 28.385912 4 CAACGCA 22775 0.0 27.708752 5 AACGCAG 23275 0.0 27.113506 6 ACGCAGA 26840 0.0 23.44157 7 GTACATG 16120 0.0 23.436583 1 CGCAGAG 27075 0.0 23.150614 8 TACATGG 16295 0.0 22.596134 2 GCAGAGT 28370 0.0 21.375769 9 ACATGGG 16355 0.0 21.349518 3 GAGTACT 16665 0.0 21.25125 12-13 CTAACGG 135 3.864742E-4 21.056618 9 AGTACTT 17325 0.0 20.769836 12-13 GTGGTAT 4730 0.0 20.744846 1 GTACTTT 17205 0.0 20.542944 14-15 TACTTTT 18295 0.0 19.565033 14-15 CATGGGA 9800 0.0 19.289366 4 >>END_MODULE