FastQCFastQC Report
Fri 27 May 2016
SRR939412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2915692
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152850.5242323263225334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105310.3611835543671965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.2839120181418339No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56250.19292161174774292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.047.4788131
GTATCAA148300.042.71711
TATCAAC195100.032.362382
ATCAACG200750.031.7719213
TCAACGC205000.031.378694
CAACGCA205350.030.9157315
AACGCAG208250.030.2708266
ACGCAGA229000.026.9995847
CGCAGAG231550.026.5983858
GTACATG141450.026.2586351
TACATGG144050.025.0416132
GCAGAGT227400.025.0357919
ACATGGG147650.023.3433233
GAGTACT134100.022.67103812-13
AGTACTT141850.022.29715712-13
AGAGTAC217250.021.06695710-11
GTACTTT149050.020.90040214-15
CAGAGTA222400.020.49182910-11
CATGGGA92050.020.2030774
TACTTTT160050.019.99761214-15
Produced by FastQC (version 0.11.5)