FastQCFastQC Report
Fri 27 May 2016
SRR939412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2915692
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206830.709368479249523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168650.5784218634890105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128320.4401013550128066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82770.2838777209664121No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41300.14164733449212055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38130.13077512988340334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91300.047.8236471
GTATCAA160400.039.2637861
ATCAACG211000.029.7446733
TATCAAC211850.029.7001512
TCAACGC213200.029.4225584
CAACGCA217150.028.9300635
AACGCAG222850.028.2326496
ACGCAGA256200.024.483537
CGCAGAG258700.024.2102768
GTACATG156200.023.7906551
GCAGAGT268550.022.6337349
TACATGG161050.022.5689642
GAGTACT160550.021.6719112-13
ACATGGG158150.021.5670853
AGTACTT165500.021.09532412-13
GTACTTT169000.020.65843614-15
CCTATAA17400.020.4567991
AGAGTAC258550.019.56516310-11
TACTTTT179100.019.41404214-15
CAGAGTA264300.019.32785610-11
Produced by FastQC (version 0.11.5)