##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2915692 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.889849133584754 31.0 31.0 31.0 28.0 34.0 2 31.142657043336538 31.0 31.0 34.0 28.0 34.0 3 31.138733446468283 31.0 31.0 34.0 28.0 34.0 4 35.05889613854961 35.0 35.0 37.0 32.0 37.0 5 34.61642656357393 35.0 35.0 37.0 30.0 37.0 6 34.677683719679585 35.0 35.0 37.0 31.0 37.0 7 34.51124295707503 35.0 35.0 37.0 30.0 37.0 8 34.40752075322085 35.0 35.0 37.0 30.0 37.0 9 35.90952096449145 37.0 35.0 39.0 30.0 39.0 10-11 35.703687323626774 37.0 35.0 39.0 30.0 39.0 12-13 35.679517589649386 37.0 35.0 39.0 30.0 39.0 14-15 36.4922882801064 38.0 35.0 40.0 30.0 41.0 16-17 36.415375320850075 38.0 35.0 40.0 30.0 41.0 18-19 36.42244122493048 38.0 35.0 40.0 30.0 41.0 20-21 36.307361168463615 38.0 34.5 40.0 29.5 41.0 22-23 36.13898021464544 38.0 34.0 40.0 29.0 41.0 24-25 35.96079455580356 38.0 34.0 40.0 29.0 41.0 26-27 35.48190926888025 38.0 34.0 40.0 27.0 41.0 28-29 35.24605033727842 38.0 33.5 40.0 27.0 41.0 30-31 34.914607750064135 37.0 33.0 40.0 26.5 41.0 32-33 34.62686199365365 37.0 33.0 40.0 25.0 41.0 34-35 34.82662657784155 37.0 33.0 40.0 26.0 41.0 36-37 34.6357981227098 37.0 33.0 40.0 25.0 41.0 38-39 34.43159205430477 37.0 33.0 40.0 25.0 41.0 40-41 34.24000528862445 37.0 33.0 40.0 24.0 41.0 42-43 34.035952700079434 37.0 33.0 40.0 24.0 41.0 44-45 33.606120090873794 37.0 32.0 40.0 23.0 41.0 46-47 33.15074997633495 37.0 31.0 40.0 21.0 41.0 48-49 32.718178394700125 36.0 31.0 39.0 18.5 40.5 50-51 32.45320664871324 36.0 31.0 39.0 18.0 40.0 52-53 32.31448074762355 36.0 31.0 39.0 18.0 40.0 54-55 31.948753503456466 35.5 30.0 39.0 15.5 40.0 56-57 31.438020373894087 35.0 29.5 39.0 12.5 40.0 58-59 30.95287962514559 35.0 28.5 38.0 9.0 40.0 60-61 30.572975986489656 34.0 28.0 38.0 8.0 40.0 62-63 30.392720150139315 34.0 28.0 38.0 7.5 40.0 64-65 29.91018152809007 34.0 28.0 37.5 6.5 40.0 66-67 29.05079994731954 33.5 27.0 36.5 2.0 39.0 68-69 25.696549566963863 31.0 16.5 35.0 2.0 38.5 70-71 24.417519751743324 29.5 12.0 35.0 2.0 38.0 72-73 25.398643786792295 30.0 18.0 35.0 2.0 37.0 74-75 26.113232639112773 31.0 21.0 35.0 2.0 37.0 76-77 25.763327367911288 30.5 21.5 34.0 2.0 36.0 78-79 26.856388809243228 32.0 24.5 35.0 2.0 36.0 80-81 26.890713250919507 32.0 25.0 35.0 2.0 36.0 82-83 26.584264215836242 32.0 24.5 35.0 2.0 36.0 84-85 26.245322379730098 32.0 24.0 34.0 2.0 35.0 86-87 25.80666887997772 31.5 23.0 34.0 2.0 35.0 88-89 25.34384255950217 31.0 20.5 34.0 2.0 35.0 90-91 24.776166001072816 31.0 19.0 34.0 2.0 35.0 92-93 24.2250894470335 31.0 17.0 34.0 2.0 35.0 94-95 23.40585493941061 30.0 7.0 34.0 2.0 35.0 96-97 22.745010961377265 30.0 2.0 34.0 2.0 35.0 98-99 21.904248116742096 29.5 2.0 34.0 2.0 35.0 100-101 20.52701691399503 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3253843752909731 1101 2 0.2707624253077512 1101 3 0.4220129063601661 1101 4 0.3753757389886516 1101 5 0.5163352076816068 1101 6 0.37770736831539864 1101 7 0.5054723746361773 1101 8 0.7464250899048608 1101 9 0.70033423488983 1101 10-11 0.7431864061289772 1101 12-13 0.7212373879951315 1101 14-15 0.7232816800959228 1101 16-17 0.7412824009715351 1101 18-19 0.5701351703608069 1101 20-21 0.7364392481860165 1101 22-23 0.738458280737774 1101 24-25 0.7092828935002302 1101 26-27 0.8049997007923366 1101 28-29 0.7636613783570212 1101 30-31 0.5286279806630532 1101 32-33 0.7838515655020899 1101 34-35 0.8659262483036514 1101 36-37 1.145272218110243 1101 38-39 1.0545094591892834 1101 40-41 0.9827293014742224 1101 42-43 0.6852369128695059 1101 44-45 1.0006709811506482 1101 46-47 0.7611389904097194 1101 48-49 0.9982676780345017 1101 50-51 0.7432188058194384 1101 52-53 0.4019791304220135 1101 54-55 0.6176041261734007 1101 56-57 0.6910462027100692 1101 58-59 0.7247885216783665 1101 60-61 0.6489490824236235 1101 62-63 0.3817904956295237 1101 64-65 0.220025899312418 1101 66-67 0.002359427816312376 1101 68-69 -6.667192361110178 1101 70-71 -7.326482547016344 1101 72-73 -4.667264970895683 1101 74-75 -2.637805816362917 1101 76-77 -0.12664574390980476 1101 78-79 0.2142981713058525 1101 80-81 0.1194152229425427 1101 82-83 -0.13303242619100075 1101 84-85 -0.16849453101637124 1101 86-87 -0.34645399264635657 1101 88-89 -0.37115459635864667 1101 90-91 -0.4500535022436516 1101 92-93 -0.5001589938729403 1101 94-95 -0.6440980454257108 1101 96-97 -0.5700684026296869 1101 98-99 -0.5345604976172815 1101 100-101 -0.5460644060330431 1102 1 0.401629738459814 1102 2 0.27862636312487865 1102 3 0.33755876415096253 1102 4 0.32582645935028154 1102 5 0.5160835424969719 1102 6 0.42971807327351996 1102 7 0.5455724255284693 1102 8 0.6242787933378153 1102 9 0.6345674310456673 1102 10-11 0.5347970473319208 1102 12-13 0.5465008574753298 1102 14-15 0.7997712683477118 1102 16-17 0.7592178396638971 1102 18-19 0.6183334973066366 1102 20-21 0.6746344732585854 1102 22-23 0.7019972385083548 1102 24-25 0.5802663371268295 1102 26-27 0.6835607132214037 1102 28-29 0.6242138493496086 1102 30-31 0.4823789603596609 1102 32-33 0.6355979521093431 1102 34-35 0.3718772810496844 1102 36-37 0.36805447859089924 1102 38-39 0.5432082942591094 1102 40-41 0.495693503899318 1102 42-43 0.2918206139136359 1102 44-45 0.35509335762099425 1102 46-47 0.29894009981376257 1102 48-49 0.4310066620901125 1102 50-51 0.33879070696216473 1102 52-53 -0.026123322075186195 1102 54-55 -0.2556725165810789 1102 56-57 -0.021856601399342424 1102 58-59 0.22026349177814453 1102 60-61 0.18046555291128996 1102 62-63 0.03390297518672014 1102 64-65 -0.1716842195454511 1102 66-67 -0.2571042816645921 1102 68-69 -6.847666108836204 1102 70-71 -7.528074349282608 1102 72-73 -1.4449809475603281 1102 74-75 -0.09095839217605928 1102 76-77 0.3130547285815908 1102 78-79 0.1042399427730949 1102 80-81 -0.25467259063027825 1102 82-83 -0.20511033374279464 1102 84-85 -0.46259555900873295 1102 86-87 -0.33881760210034173 1102 88-89 -0.3539409973307919 1102 90-91 -0.40426407050363977 1102 92-93 -0.5018331665536095 1102 94-95 -0.48087469776743674 1102 96-97 -0.6292524142206659 1102 98-99 -0.5583936899382209 1102 100-101 -0.531356972802211 1103 1 0.2568196613189073 1103 2 0.20161565453522456 1103 3 0.28992751069264244 1103 4 0.28938796050576343 1103 5 0.3397010277685908 1103 6 0.3326450335313069 1103 7 0.31028453046613436 1103 8 0.4389453298088668 1103 9 0.47876802994551326 1103 10-11 0.5076838096923737 1103 12-13 0.39308973225969623 1103 14-15 0.5256627014759871 1103 16-17 0.5469705005368652 1103 18-19 0.5393759573528527 1103 20-21 0.6377647014263985 1103 22-23 0.6020456550041473 1103 24-25 0.5632513971795561 1103 26-27 0.6675120688832834 1103 28-29 0.6315800733511665 1103 30-31 0.5902358591000123 1103 32-33 0.7213093897944631 1103 34-35 0.6266395926729018 1103 36-37 0.8944414687936799 1103 38-39 0.8022487525476834 1103 40-41 0.7664169846791822 1103 42-43 0.5130514507459694 1103 44-45 0.8127594384073689 1103 46-47 0.6226959179304856 1103 48-49 0.6868288474235769 1103 50-51 0.37669187221295175 1103 52-53 0.2964200493403695 1103 54-55 0.11802349292320358 1103 56-57 0.4498767091497555 1103 58-59 0.5505148506949205 1103 60-61 0.5576422609150633 1103 62-63 0.25888710759566536 1103 64-65 0.1894397755071111 1103 66-67 0.10918074909411857 1103 68-69 -6.701714785723226 1103 70-71 -7.773663972691779 1103 72-73 -1.2200171582168409 1103 74-75 0.30971252212267686 1103 76-77 0.6743455367708933 1103 78-79 0.4354840432841094 1103 80-81 0.25627604719805674 1103 82-83 0.059430889445046375 1103 84-85 -0.06166221916561909 1103 86-87 -0.00598674248376696 1103 88-89 -0.12832419558617048 1103 90-91 -0.14894029027384548 1103 92-93 -0.22051298297778388 1103 94-95 -0.06468405883243733 1103 96-97 -0.01880787755432678 1103 98-99 -0.05083827542791752 1103 100-101 -0.029542371363550757 1104 1 0.2784720611863669 1104 2 0.2587284621903301 1104 3 0.2204635586752346 1104 4 0.2073360902605259 1104 5 0.415468664738583 1104 6 0.28269446762164563 1104 7 0.33709162482823984 1104 8 0.4292141251597812 1104 9 0.3967678072573477 1104 10-11 0.4273225585068232 1104 12-13 0.2095698640481629 1104 14-15 0.4947492909530311 1104 16-17 0.5229540511253035 1104 18-19 0.47179680579477434 1104 20-21 0.4392153941073431 1104 22-23 0.4061229948732432 1104 24-25 0.42325096790116845 1104 26-27 0.6774143141434479 1104 28-29 0.5391044516688552 1104 30-31 0.5259718770331219 1104 32-33 0.5353218415509531 1104 34-35 0.29450958034455965 1104 36-37 0.6619124287809015 1104 38-39 0.6555512449112229 1104 40-41 0.6507962145805806 1104 42-43 0.475159247917297 1104 44-45 0.6851708561395213 1104 46-47 0.6739598074342297 1104 48-49 0.6977479458852827 1104 50-51 0.40435014514547163 1104 52-53 0.25099300453309326 1104 54-55 0.21259855426305663 1104 56-57 0.3538756386367581 1104 58-59 0.4858953724559818 1104 60-61 0.5902345524165575 1104 62-63 0.37051558860747136 1104 64-65 0.2899868371635641 1104 66-67 0.046961876137718406 1104 68-69 -6.811846829074977 1104 70-71 -4.037871075371147 1104 72-73 0.21776899943296968 1104 74-75 0.8831583666407248 1104 76-77 1.0722000838352344 1104 78-79 0.4936737360415151 1104 80-81 0.41039504048655573 1104 82-83 0.3133346398625889 1104 84-85 0.2276271506939409 1104 86-87 0.15190520546086006 1104 88-89 0.0062864422007926635 1104 90-91 0.09331291153931787 1104 92-93 0.08560342927435727 1104 94-95 0.10756155011909385 1104 96-97 0.06392949663021241 1104 98-99 0.34792647598635185 1104 100-101 0.3684644353820197 1105 1 0.16452325273330004 1105 2 0.22352595967723587 1105 3 0.13210381580448427 1105 4 0.20658934216707792 1105 5 0.34069843338271966 1105 6 0.1769290886167596 1105 7 0.345898698528039 1105 8 0.3905358995179 1105 9 0.38923305852900825 1105 10-11 0.31432513584589117 1105 12-13 0.39161134711406476 1105 14-15 0.49111777485789077 1105 16-17 0.35955977682387186 1105 18-19 0.4164484493208178 1105 20-21 0.49499711767449384 1105 22-23 0.5048614875455826 1105 24-25 0.5686931969593445 1105 26-27 0.6749855405335907 1105 28-29 0.6700934110587582 1105 30-31 0.7102042400222288 1105 32-33 0.7448008373712796 1105 34-35 0.5859838126225156 1105 36-37 0.4834473815800777 1105 38-39 0.42853670675375355 1105 40-41 0.3888245785371254 1105 42-43 0.33555991643823546 1105 44-45 0.3661350624929227 1105 46-47 0.32379721210669743 1105 48-49 0.6956113903742391 1105 50-51 0.4067158551986054 1105 52-53 0.4061230713474018 1105 54-55 0.6060500061892604 1105 56-57 0.6532343271415186 1105 58-59 0.810412263096687 1105 60-61 0.7024129518965587 1105 62-63 0.4801477792700979 1105 64-65 0.6103061985730136 1105 66-67 0.5747249102175509 1105 68-69 -2.8914264242589063 1105 70-71 -3.1467723901164 1105 72-73 0.7558385554655302 1105 74-75 1.274784471079645 1105 76-77 1.071696378437938 1105 78-79 0.6720130340378425 1105 80-81 0.3824854722097477 1105 82-83 0.39386118040843954 1105 84-85 0.4996752093756456 1105 86-87 0.4410386696766615 1105 88-89 0.36828415396207603 1105 90-91 0.502603708700466 1105 92-93 0.5735622579833439 1105 94-95 0.5704212249662284 1105 96-97 0.4826206373105997 1105 98-99 0.3966601030809116 1105 100-101 0.5887099628295509 1106 1 0.0632515196129475 1106 2 0.20101182930923045 1106 3 0.23247473796459062 1106 4 0.22496189239132036 1106 5 0.30156345765009007 1106 6 0.28397430579436644 1106 7 0.3347257243927544 1106 8 0.35090328908600554 1106 9 0.4733410022532851 1106 10-11 0.49846763753539136 1106 12-13 0.22710468126654604 1106 14-15 0.2639705732851212 1106 16-17 0.47277282283972255 1106 18-19 0.3201415438714079 1106 20-21 0.47641261193019346 1106 22-23 0.36666149676104 1106 24-25 0.5192238537142657 1106 26-27 0.6455555933688757 1106 28-29 0.5236594090927937 1106 30-31 0.5834198970843474 1106 32-33 0.7827278202305266 1106 34-35 0.650414066969148 1106 36-37 0.6952677415972772 1106 38-39 0.682498585478335 1106 40-41 0.6369964621766186 1106 42-43 0.5335789770759405 1106 44-45 0.6930561013821546 1106 46-47 0.8910639074436801 1106 48-49 0.8795323371088983 1106 50-51 0.8047544986832733 1106 52-53 0.8108632722443687 1106 54-55 0.7816271242085513 1106 56-57 0.846371115247436 1106 58-59 1.1798073451142592 1106 60-61 1.2726777427056746 1106 62-63 0.8437448026773602 1106 64-65 0.9041299222321619 1106 66-67 1.0611977706955251 1106 68-69 -2.184508687575242 1106 70-71 -2.2629850675139522 1106 72-73 1.4604067344911478 1106 74-75 1.8531888146318956 1106 76-77 1.7865866376994717 1106 78-79 1.4470498958674192 1106 80-81 1.2959411919369686 1106 82-83 0.990482947318803 1106 84-85 0.8849972896115617 1106 86-87 0.9788101416923887 1106 88-89 1.028320938996174 1106 90-91 1.1371816407518303 1106 92-93 1.0000962476620323 1106 94-95 1.2372240756143817 1106 96-97 1.3131741023225807 1106 98-99 1.4506327331724513 1106 100-101 1.659187477113509 1107 1 0.019974559523532776 1107 2 0.06486897029341776 1107 3 0.12496665440359322 1107 4 -0.10725771497623526 1107 5 0.22604484784231005 1107 6 0.24046749888699992 1107 7 0.19768293928846958 1107 8 0.0869416313045619 1107 9 0.3416570076221106 1107 10-11 0.16020164535919434 1107 12-13 0.13412150310372084 1107 14-15 0.16769671387434926 1107 16-17 0.03787703645222251 1107 18-19 0.11917112732833601 1107 20-21 0.1999229710156598 1107 22-23 0.25697232668423453 1107 24-25 0.19404786316052736 1107 26-27 0.5449973032325417 1107 28-29 0.3715181258182625 1107 30-31 0.21753490079630922 1107 32-33 0.41716444560398 1107 34-35 0.3726026019968174 1107 36-37 0.38501243545442776 1107 38-39 0.45573155100213825 1107 40-41 0.33020934375920774 1107 42-43 0.06563523341619515 1107 44-45 0.28914958635156296 1107 46-47 0.4221926847569364 1107 48-49 0.2769742567436282 1107 50-51 0.2924609715957658 1107 52-53 0.2599207568925692 1107 54-55 0.19249582636477314 1107 56-57 0.5973684496798839 1107 58-59 0.5620480320161825 1107 60-61 0.5905008884850993 1107 62-63 0.3992820009880482 1107 64-65 0.18216488353104054 1107 66-67 0.4420951238107058 1107 68-69 -2.505315335187703 1107 70-71 -2.675927717250346 1107 72-73 1.2177209641806357 1107 74-75 1.3868831904728225 1107 76-77 0.8853669950969341 1107 78-79 0.9278858540705315 1107 80-81 0.7208972301480827 1107 82-83 0.7400975134614249 1107 84-85 0.6662812442551207 1107 86-87 0.6383236284973002 1107 88-89 0.3919223190603809 1107 90-91 0.49961780807047873 1107 92-93 0.527629315285342 1107 94-95 0.752299527646457 1107 96-97 0.6380357435517716 1107 98-99 0.8666594683315587 1107 100-101 0.9998922628438329 1108 1 -0.016085245324600095 1108 2 -0.024874167699280747 1108 3 -0.04741903334083375 1108 4 -0.023366154679962392 1108 5 -0.08563351610698788 1108 6 -0.09404479133480947 1108 7 0.09714356808505187 1108 8 0.17509975289338087 1108 9 0.22956738256857534 1108 10-11 0.2678846018080563 1108 12-13 0.043575264964076155 1108 14-15 0.08164041170901726 1108 16-17 0.2629766321964837 1108 18-19 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 50.0 4 129.0 5 321.0 6 852.0 7 1926.0 8 3850.0 9 6238.0 10 9916.0 11 13797.0 12 18060.0 13 22257.0 14 26887.0 15 33352.0 16 39290.0 17 43377.0 18 40043.0 19 35243.0 20 34752.0 21 35382.0 22 37114.0 23 41634.0 24 49500.0 25 60180.0 26 74018.0 27 92067.0 28 111249.0 29 131982.0 30 153915.0 31 177510.0 32 203707.0 33 234845.0 34 270255.0 35 299642.0 36 294892.0 37 227882.0 38 85313.0 39 4253.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.90882514574104 20.119124476604934 15.359428246421567 22.612622131232456 2 16.29301248265916 21.558829865623082 37.59291327454029 24.55524437717747 3 18.94522682366612 25.39024357619615 30.305942458000352 25.358587142137388 4 13.524269801551078 17.674371022276112 35.66426508466872 33.13709409150408 5 13.975550229585293 37.729156577580895 33.089949144148285 15.205344048685527 6 32.08140640369422 36.14802249345953 16.58580535941382 15.184765743432434 7 28.27723230025668 33.672143696933695 19.97940797587674 18.071216026932884 8 27.393394089636352 33.93163612617519 19.242155893009276 19.43281389117918 9 26.930896679073097 16.311496550390096 19.91801603187168 36.83959073866513 10-11 25.480548832137583 26.88963965547668 26.056903869310133 21.572907643075602 12-13 26.35964292524725 24.236990738390748 27.300157218252135 22.103209118109866 14-15 23.212139691023605 25.32805248290972 25.987981583788685 25.471826242277988 16-17 22.4019424671521 28.770025176001358 26.359501501111147 22.46853085573539 18-19 22.080873865640996 26.40436384065033 29.911820141280526 21.60294215242815 20-21 22.78599728640748 25.603904664827425 30.084933525214595 21.5251645235505 22-23 22.556915880301865 25.52819072159556 30.102331030672214 21.812562367430356 24-25 22.47491623883606 25.91529339858961 29.88057442400427 21.72921593857006 26-27 22.208977369308116 26.231205399372616 29.747362290686162 21.812454940633106 28-29 22.036689488273318 26.279916917553432 30.035604312988696 21.647789281184558 30-31 21.977637170525707 26.170491923600153 29.892171191930274 21.95969971394387 32-33 21.98638017159317 26.248016968966542 29.587191715950556 22.178411143489736 34-35 22.539375598181717 26.200260563282455 29.465018684791655 21.79534515374417 36-37 22.49692124303686 25.958791428054063 29.355078726068296 22.189208602840782 38-39 22.213717566032628 26.232382187004287 29.623313919486293 21.930586327476792 40-41 22.215153224109592 26.019540411766943 29.82135324079143 21.94395312333203 42-43 22.256636211258947 26.337428571330495 29.156764969084048 22.249170248326507 44-45 22.253298540671647 26.551716325845497 28.94568555205203 22.249299581430822 46-47 22.34009907651244 26.63211597553843 28.542541389432753 22.48524355851638 48-49 22.550094732072985 26.838608811455718 28.145842772217417 22.465453684253884 50-51 22.387751305480787 27.076447252535278 28.018583236713685 22.51721820527025 52-53 22.873687846382214 26.797695712654896 27.831134921401617 22.497481519561273 54-55 22.268720059665775 26.814769646975783 27.977579696342108 22.938930597016334 56-57 22.480953780992238 27.284474196734482 27.680047535355623 22.554524486917657 58-59 22.570353702018 26.72791457128268 27.81942674170513 22.882304984994196 60-61 22.52275197394159 26.643910833769397 28.255650840317614 22.577686351971405 62-63 22.535958817777445 26.856367429715807 28.056761032875606 22.550912719631146 64-65 22.43109660623668 26.638581098578456 27.974975089993293 22.955347205191572 66-67 22.115205259930644 27.0588770776331 28.078297805098824 22.747619857337433 68-69 22.19482295488201 26.471308124817444 28.745111444639036 22.58875747566151 70-71 21.572612629819414 26.615949058179407 29.04728366862212 22.764154643379054 72-73 22.12985479709973 26.799460974891964 28.040629964082203 23.030054263926107 74-75 22.240911219294915 27.019411143885012 27.567166716968533 23.17251091985154 76-77 21.678994323040783 27.055240856236452 28.219018354625153 23.046746466097616 78-79 22.04380781393479 27.08831641842856 27.69673159927403 23.171144168362616 80-81 21.89364536473403 27.37173841166359 27.61645614425004 23.11816007935234 82-83 22.18434037482965 27.192855644781112 27.237048275261266 23.385755705127977 84-85 21.98330582409809 27.07033932820304 27.173128121583673 23.773226726115197 86-87 22.2633397642015 26.91867095391211 27.4071768488746 23.41081243301179 88-89 21.951549519528562 26.882460957622893 27.29913934254743 23.866850180301117 90-91 21.781343677758798 27.176685256449833 27.424491867827328 23.61747919796404 92-93 21.939262253403495 26.861631569154206 27.449801796734253 23.749304380708043 94-95 21.610376380098405 26.686567645737856 27.48979765907783 24.213258315085906 96-97 21.88751943882378 26.336151623254366 27.52667825776674 24.249650680155117 98-99 21.623792833528512 26.442202478100008 27.252702811170593 24.68130187720089 100-101 21.460604166745277 26.32210998377194 27.636990917981247 24.58029493150154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6090.0 1 4116.5 2 2297.0 3 2588.5 4 2499.5 5 2500.5 6 3874.0 7 7049.0 8 10250.0 9 11376.0 10 9914.0 11 7863.0 12 7068.5 13 6817.0 14 6639.0 15 6520.5 16 6482.5 17 6476.0 18 6538.5 19 6764.5 20 7172.5 21 7789.0 22 8766.0 23 9997.5 24 11483.0 25 13587.5 26 16048.0 27 18617.0 28 21124.0 29 24105.0 30 27922.5 31 32566.0 32 38169.5 33 42341.5 34 45620.0 35 49211.5 36 54001.5 37 59921.0 38 64125.0 39 65650.5 40 67995.5 41 74374.0 42 80936.5 43 86158.0 44 89613.0 45 92724.5 46 95634.5 47 99479.5 48 109593.0 49 115760.5 50 114969.5 51 112952.5 52 109374.0 53 109264.5 54 109144.0 55 103121.0 56 95356.5 57 86512.0 58 77141.0 59 68889.0 60 60336.0 61 50477.5 62 40564.5 63 31504.5 64 23805.5 65 18395.0 66 13953.5 67 10567.5 68 7547.5 69 5052.5 70 3685.0 71 2858.5 72 2319.0 73 1890.5 74 1516.5 75 1191.0 76 836.0 77 514.5 78 295.0 79 176.0 80 118.0 81 82.0 82 49.5 83 29.5 84 12.5 85 5.0 86 6.0 87 5.5 88 1.5 89 1.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020235333498874367 2 0.020852682656467145 3 4.4586328048367243E-4 4 0.09435152958542946 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01034059838967902 12-13 0.0 14-15 0.0 16-17 6.344977453036878E-4 18-19 1.2004011397637337E-4 20-21 0.0 22-23 2.572288156636572E-4 24-25 0.0037898378841112164 26-27 0.0032239344896511702 28-29 0.0011661039643419127 30-31 1.886344648200153E-4 32-33 5.659033944600458E-4 34-35 0.0029324084985656923 36-37 0.08864104987769626 38-39 0.09975333471436626 40-41 0.08042687636417016 42-43 0.08474832046731959 44-45 0.08347932497671222 46-47 0.08190165490730845 48-49 0.07586535203306796 50-51 0.07565956898053704 52-53 0.059419856418304814 54-55 0.050022430352725876 56-57 0.027746414916253154 58-59 0.02241320413816 60-61 0.013564532879330191 62-63 0.0022293164024183624 64-65 0.006756543558098729 66-67 0.15869303067676557 68-69 0.7219040968661985 70-71 0.8770302212990947 72-73 0.35830259163176353 74-75 0.055509978420217224 76-77 0.19794614794703966 78-79 0.014164733449212055 80-81 0.0030181514371202447 82-83 0.0016291158325364955 84-85 1.5433728939819432E-4 86-87 0.0022979107532620042 88-89 4.8016045590549346E-4 90-91 0.0038412836472439477 92-93 0.00426999834001671 94-95 0.07776884526897902 96-97 0.03770974437629215 98-99 0.013513087116197458 100-101 0.012724252081495576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2915692.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.93861881462807 #Duplication Level Percentage of deduplicated Percentage of total 1 64.50729150081666 22.537956685111308 2 14.492432162889363 10.126911260720945 3 6.72492702694441 7.048789858518023 4 3.777892160864315 5.279773365248394 5 2.5037103006658996 4.3738089908611855 6 1.6699911171830193 3.500830984024343 7 1.1971410807057326 2.927851912027664 8 0.8598902066550996 2.403470092220343 9 0.6664893089360321 2.095759431804682 >10 3.253334997944193 19.535429059879338 >50 0.19677450842486233 4.749807038717725 >100 0.1363137675640628 9.23390248227636 >500 0.010408938277198488 2.4592749922307853 >1k 0.0030025783491867647 1.6752779309469643 >5k 1.0008594497289213E-4 0.28943548957268145 >10k+ 3.002578349186764E-4 1.7617204258392767 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20683 0.709368479249523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16865 0.5784218634890105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12832 0.4401013550128066 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8277 0.2838777209664121 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4130 0.14164733449212055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3813 0.13077512988340334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.4297175421820964E-5 0.0 12-13 0.0 0.0 0.0 3.4297175421820964E-5 0.0 14-15 0.0 0.0 0.0 5.1445763132731445E-5 0.0 16-17 0.0 0.0 0.0 1.2004011397637337E-4 0.0 18-19 0.0 0.0 0.0 1.714858771091048E-4 0.0 20-21 0.0 0.0 0.0 3.9441751735094105E-4 0.0 22-23 0.0 0.0 0.0 0.0013032926660291964 0.0 24-25 0.0 0.0 0.0 0.0022293164024183624 0.0 26-27 0.0 0.0 0.0 0.0036012034192912006 0.0 28-29 0.0 0.0 0.0 0.006585057680989624 0.0 30-31 0.0 0.0 0.0 0.015296540238132147 0.0 32-33 0.0 0.0 0.0 0.027849306442518616 0.0 34-35 0.0 0.0 0.0 0.046266889644036475 0.0 36-37 0.0 0.0 0.0 0.07519655711234247 0.0 38-39 0.0 0.0 0.0 0.1176564602845568 0.0 40-41 0.0 0.0 0.0 0.16354608099895326 0.0 42-43 0.0 0.0 0.0 0.2106018056776916 0.0 44-45 0.0 0.0 0.0 0.2664719044398379 0.0 46-47 0.0 0.0 0.0 0.33631810218637637 0.0 48-49 0.0 0.0 0.0 0.4108115672025715 0.0 50-51 0.0 0.0 0.0 0.48904342433974507 0.0 52-53 0.0 0.0 0.0 0.5684413854412607 0.0 54-55 0.0 1.7148587710910482E-5 0.0 0.6537213121276184 0.0 56-57 0.0 3.4297175421820964E-5 0.0 0.7563213124019958 3.4297175421820964E-5 58-59 0.0 3.4297175421820964E-5 0.0 0.8579095460014294 3.4297175421820964E-5 60-61 0.0 3.4297175421820964E-5 0.0 0.9645566129755818 3.4297175421820964E-5 62-63 0.0 3.4297175421820964E-5 0.0 1.0658018748207971 5.1445763132731445E-5 64-65 0.0 3.4297175421820964E-5 0.0 1.1790168508882282 6.859435084364193E-5 66-67 0.0 3.4297175421820964E-5 0.0 1.2999658400132799 6.859435084364193E-5 68-69 0.0 3.4297175421820964E-5 0.0 1.4321643026766888 6.859435084364193E-5 70-71 0.0 3.4297175421820964E-5 0.0 1.6055365244339939 6.859435084364193E-5 72-73 0.0 3.4297175421820964E-5 0.0 1.7952856474552181 6.859435084364193E-5 74-75 0.0 3.4297175421820964E-5 0.0 2.0008972141090346 6.859435084364193E-5 76-77 0.0 3.4297175421820964E-5 0.0 2.2367074437217647 6.859435084364193E-5 78-79 3.4297175421820964E-5 3.4297175421820964E-5 0.0 2.5018932040832844 6.859435084364193E-5 80-81 3.4297175421820964E-5 3.4297175421820964E-5 0.0 2.7924588742569516 6.859435084364193E-5 82-83 3.4297175421820964E-5 3.4297175421820964E-5 0.0 3.0946512868986162 6.859435084364193E-5 84-85 3.4297175421820964E-5 3.4297175421820964E-5 0.0 3.4129976691639583 6.859435084364193E-5 86-87 3.4297175421820964E-5 3.4297175421820964E-5 0.0 3.744497018203569 6.859435084364193E-5 88-89 3.4297175421820964E-5 3.4297175421820964E-5 0.0 4.098220936916519 6.859435084364193E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9130 0.0 47.823647 1 GTATCAA 16040 0.0 39.263786 1 ATCAACG 21100 0.0 29.744673 3 TATCAAC 21185 0.0 29.700151 2 TCAACGC 21320 0.0 29.422558 4 CAACGCA 21715 0.0 28.930063 5 AACGCAG 22285 0.0 28.232649 6 ACGCAGA 25620 0.0 24.48353 7 CGCAGAG 25870 0.0 24.210276 8 GTACATG 15620 0.0 23.790655 1 GCAGAGT 26855 0.0 22.633734 9 TACATGG 16105 0.0 22.568964 2 GAGTACT 16055 0.0 21.67191 12-13 ACATGGG 15815 0.0 21.567085 3 AGTACTT 16550 0.0 21.095324 12-13 GTACTTT 16900 0.0 20.658436 14-15 CCTATAA 1740 0.0 20.456799 1 AGAGTAC 25855 0.0 19.565163 10-11 TACTTTT 17910 0.0 19.414042 14-15 CAGAGTA 26430 0.0 19.327856 10-11 >>END_MODULE