FastQCFastQC Report
Fri 27 May 2016
SRR939411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814097
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160920.8870528973919255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114650.6319948712775557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86480.47671100277438305No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70420.3881821093359396No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28600.1576541937944884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.15710295535464752No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27140.1496061125728117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23380.12887954723479506No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21760.11994948450937297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.11327949938729846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG3950.029.8685469
GTATCAA220150.028.8561
GTACATG108550.027.8283711
TACATGG114100.026.5136642
GTATCGA1401.1638176E-525.0894491
ACATGGG115750.024.6777943
GGTATCA153700.024.4854741
GAGTACT129100.024.44856512-13
TATCAAC261150.024.1976552
CATGGGG39300.024.00364
ATCAACG270300.023.458213
TCAACGC271100.023.3686164
CAACGCA269700.023.1421595
AGTACTT137000.023.09123612-13
CATGGGA70250.023.0395014
AACGCAG270600.022.916146
GCGGAGT3450.022.7982149
TAGGCAT4950.022.4636945
ATCGACG1302.769557E-422.1201973
GTACTTT145750.021.95296114-15
Produced by FastQC (version 0.11.5)