FastQCFastQC Report
Fri 27 May 2016
SRR939411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814097
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223601.232569151484182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183641.012294270923771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140720.7757027325440701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110040.6065827792008917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.2573732275617015No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45430.2504276232197066No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39600.21829042217698394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37350.2058875572805644No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA34510.19023238558908373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.1449757096781484No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26020.143432242046594No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22760.12546186890778166No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.12287104824052959No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.1219890667367842No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG18180.10021514836306988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG4850.030.2834429
GGTATCA159500.027.8142951
TAGGCAT5300.026.8182645
GTATCAA225400.026.7687051
GAGTGCA7450.024.1664339
GTACATG128250.023.5045871
GAGTACT145850.022.98292412-13
TTAGGCA6700.022.6287424
CCCTATA5900.022.4898742
CTTTAGG5900.022.4898742
TACATGG132450.022.4692552
AGGCTTA8450.022.4278966
TATCAAC275550.021.7899992
ATCAACG275850.021.6756253
TCAACGC276450.021.6114444
AGTACTT152850.021.5584212-13
CTTACTA8850.021.4142079
CAACGCA278650.021.4068095
ACATGGG132600.021.40273
AACGCAG281000.021.2277856
Produced by FastQC (version 0.11.5)