FastQCFastQC Report
Fri 27 May 2016
SRR939410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820331
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160820.8834656993700596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108830.5978583015946002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87490.4806268750024034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69630.38251285068484797No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30630.16826610105524764No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.15848765966189665No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25820.14184233526759693No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.11992324472856859No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20770.11410012794376408No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.11036454359124796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA203100.030.0906811
GTACATG114650.028.2797931
TACATGG117950.027.0522272
GGTATCA141000.027.0002121
ACATGGG122500.025.5739613
TATCAAC249250.024.2092462
GAGTACT127150.024.1595112-13
ATCAACG257950.023.7863463
CATGGGA73100.023.7379974
TCAACGC259850.023.5010054
CAACGCA260000.023.2411925
CATTCCG4650.022.9634639
AGTACTT134700.022.87679312-13
AACGCAG262800.022.8448036
CATGGGT11100.022.7621334
TAGGCAT5650.022.3530245
CATGGGG41500.022.0553114
GTACTTT143850.021.88411514-15
ATGGGAT27300.021.5295095
ACGCAGA281600.021.113437
Produced by FastQC (version 0.11.5)