FastQCFastQC Report
Fri 27 May 2016
SRR939410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1820331
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218411.1998367329897694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176150.9676811524936948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139770.767827389634083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107540.590771678337621No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.2589638917317785No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43970.24154947644137248No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38080.2091927237409021No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36100.19831558106739927No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33500.18403246442542592No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25420.13964493270729336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.13706298469893663No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.12492233555325928No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22370.12288973818497845No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.12069233562467487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT4400.030.171385
CATATAG3300.028.7583483
GGTATCA171650.026.2728311
TTAGGCA5650.026.0402284
GTACATG132900.025.7535651
CATGGGT10450.025.433334
GTATCAA235950.024.7866481
TACATGG136100.024.6201112
ACATGGG134000.024.22143
CATTCCG4950.023.945549
GAGTACT149200.022.74963212-13
CATGGGG42750.021.9816654
GTGTACG1954.2492684E-721.9096241
CAGCGTA1954.300182E-721.8825389
CATGGGA80750.021.8641154
AGTACTT154450.021.70009812-13
ATGGGTA7650.021.6918415
GTGGTAC4250.021.2222651
TAGATAT3800.021.2107077
ATGGGAT27750.021.0151215
Produced by FastQC (version 0.11.5)