##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939410_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1820331 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.903130804232855 31.0 31.0 31.0 28.0 34.0 2 31.151507610429093 31.0 31.0 34.0 28.0 34.0 3 31.1334696821622 31.0 31.0 34.0 28.0 34.0 4 35.05590192113412 35.0 35.0 37.0 32.0 37.0 5 34.6527345850837 35.0 35.0 37.0 32.0 37.0 6 34.72201264495303 35.0 35.0 37.0 32.0 37.0 7 34.55871047628151 35.0 35.0 37.0 30.0 37.0 8 34.46412932593029 35.0 35.0 37.0 30.0 37.0 9 35.91740842736843 37.0 35.0 39.0 30.0 39.0 10-11 35.74119075047341 37.0 35.0 39.0 30.0 39.0 12-13 35.73212591556151 37.0 35.0 39.0 30.0 39.0 14-15 36.466903546662664 38.0 35.0 40.0 30.0 41.0 16-17 36.41379782028653 38.0 35.0 40.0 30.0 41.0 18-19 36.51487037247621 38.0 35.0 40.0 30.0 41.0 20-21 36.44302217563729 38.0 35.0 40.0 30.0 41.0 22-23 36.26170048194531 38.0 34.0 40.0 30.0 41.0 24-25 36.089841627703976 38.0 34.0 40.0 29.0 41.0 26-27 35.5804919544852 38.0 34.0 40.0 27.0 41.0 28-29 35.37005412751857 38.0 34.0 40.0 27.0 41.0 30-31 34.99566782085236 37.0 33.0 40.0 27.0 41.0 32-33 34.604956461215025 37.0 33.0 40.0 25.5 41.0 34-35 34.69067383898862 37.0 33.0 40.0 26.0 41.0 36-37 34.42549322073843 37.0 33.0 40.0 25.0 41.0 38-39 34.184966360513556 37.0 33.0 40.0 24.0 41.0 40-41 33.98503541388901 37.0 33.0 40.0 24.0 41.0 42-43 33.74167005890687 37.0 33.0 40.0 23.5 41.0 44-45 33.23668277912094 37.0 32.0 40.0 20.5 41.0 46-47 32.666277451738175 36.0 31.0 40.0 18.0 41.0 48-49 32.150656391612294 36.0 30.5 39.0 14.0 41.0 50-51 31.873070062532584 36.0 30.0 39.0 10.5 40.0 52-53 31.73545003628461 36.0 30.0 39.0 9.0 40.0 54-55 31.385295037001512 35.0 30.0 39.0 8.0 40.0 56-57 30.893591055692617 35.0 28.5 39.0 7.0 40.0 58-59 30.4596059727599 35.0 28.0 38.0 3.5 40.0 60-61 30.090439870551016 34.5 28.0 38.0 2.0 40.0 62-63 29.91776358255724 34.0 28.0 38.0 2.0 40.0 64-65 29.464476240859494 34.0 27.0 38.0 2.0 40.0 66-67 28.64701035141411 33.5 26.0 37.0 2.0 39.5 68-69 25.419548697462165 31.0 15.5 35.5 2.0 39.0 70-71 24.21083198605089 29.5 10.5 35.0 2.0 38.0 72-73 25.13868878791824 30.5 16.0 35.0 2.0 37.5 74-75 25.805859483797178 31.0 20.5 35.0 2.0 37.0 76-77 25.39693385433748 30.5 21.0 34.0 2.0 36.0 78-79 26.40387105422036 32.0 23.5 35.0 2.0 36.0 80-81 26.395751377084718 32.0 24.0 35.0 2.0 36.0 82-83 26.0835172284601 32.0 23.0 35.0 2.0 36.0 84-85 25.734838883697527 32.0 21.5 34.5 2.0 35.5 86-87 25.30212554749658 31.5 20.0 34.0 2.0 35.0 88-89 24.82141269911901 31.0 19.0 34.0 2.0 35.0 90-91 24.245062024434016 31.0 15.0 34.0 2.0 35.0 92-93 23.740466706329784 31.0 8.5 34.0 2.0 35.0 94-95 22.950359577461462 30.0 2.0 34.0 2.0 35.0 96-97 22.302931719560895 30.0 2.0 34.0 2.0 35.0 98-99 21.51556749843847 29.0 2.0 34.0 2.0 35.0 100-101 20.179334417751498 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.14492078979293055 1101 2 0.18367832551933816 1101 3 0.2924918699540129 1101 4 0.30752684680982867 1101 5 0.45760379997150835 1101 6 0.3599684393924676 1101 7 0.46087248616655785 1101 8 0.6017011940899835 1101 9 0.6426153030407207 1101 10-11 0.7822083050981874 1101 12-13 0.6395077399851843 1101 14-15 0.7646003959486336 1101 16-17 0.8402725121668269 1101 18-19 0.46937848485878675 1101 20-21 0.7668846054844778 1101 22-23 0.7244100948892012 1101 24-25 0.6310224321681162 1101 26-27 0.8071584182431693 1101 28-29 0.7008440964095612 1101 30-31 0.4965564357515433 1101 32-33 0.9006659619250641 1101 34-35 0.8215508731769106 1101 36-37 0.9121057814594735 1101 38-39 1.136278788617041 1101 40-41 1.1284444087043326 1101 42-43 0.6546451374627864 1101 44-45 0.8118589350436878 1101 46-47 0.6871679876429866 1101 48-49 0.760508727183911 1101 50-51 0.5711939910228523 1101 52-53 0.4160092660518693 1101 54-55 0.4573619054988072 1101 56-57 0.48837369689239196 1101 58-59 0.4416478149815788 1101 60-61 0.24573687910599418 1101 62-63 -0.12563426187293203 1101 64-65 0.040185498811510456 1101 66-67 0.0597202089327169 1101 68-69 -6.44415144698802 1101 70-71 -7.281364646054186 1101 72-73 -4.492475002247236 1101 74-75 -2.5067690270449035 1101 76-77 -0.10005240737321941 1101 78-79 0.06314613061928043 1101 80-81 -0.07060961791779263 1101 82-83 -0.31592594565855947 1101 84-85 -0.34065864584183814 1101 86-87 -0.42608123093353 1101 88-89 -0.3622798385263586 1101 90-91 -0.3356473719170161 1101 92-93 -0.5293389046528496 1101 94-95 -0.5749721947003046 1101 96-97 -0.5888519592814987 1101 98-99 -0.7100013000641674 1101 100-101 -0.3149091137617219 1102 1 0.34205781944163505 1102 2 0.3180933081439754 1102 3 0.3639960015256065 1102 4 0.32411301103481094 1102 5 0.4906833447272305 1102 6 0.4974207394031538 1102 7 0.5330352740792108 1102 8 0.5399018593274931 1102 9 0.5987356283878285 1102 10-11 0.5772616665111698 1102 12-13 0.6444399755219834 1102 14-15 0.6766209550890636 1102 16-17 0.6811507691711896 1102 18-19 0.5403175529436695 1102 20-21 0.6815277874473225 1102 22-23 0.6588825548546566 1102 24-25 0.41203659570692963 1102 26-27 0.6427003990887314 1102 28-29 0.5960033768827984 1102 30-31 0.5524389219661501 1102 32-33 0.6729741019680233 1102 34-35 0.49312370244303594 1102 36-37 0.6934823138738864 1102 38-39 0.8806003725857465 1102 40-41 0.7241530819298703 1102 42-43 0.5874586843136953 1102 44-45 0.5921151852662092 1102 46-47 0.778045705207262 1102 48-49 0.8007026774712713 1102 50-51 0.46990804678154063 1102 52-53 0.351997822369956 1102 54-55 -0.036609716716199614 1102 56-57 0.07248697067873167 1102 58-59 0.1505531346160076 1102 60-61 0.11769902227986151 1102 62-63 -0.2077534953933089 1102 64-65 -0.2573804396470045 1102 66-67 -0.24673637638998258 1102 68-69 -6.7182564619466945 1102 70-71 -7.447533904011195 1102 72-73 -1.6454483420394617 1102 74-75 -0.05918668960786633 1102 76-77 0.3265443622437907 1102 78-79 -0.03923474213003786 1102 80-81 -0.2848508091309583 1102 82-83 -0.450976388700699 1102 84-85 -0.46343574812075516 1102 86-87 -0.6526029104910194 1102 88-89 -0.7708086020110123 1102 90-91 -0.7952477600980998 1102 92-93 -0.6388701905593308 1102 94-95 -0.42580136805153046 1102 96-97 -0.6490530679387554 1102 98-99 -0.8562267838610076 1102 100-101 -0.9220386775596836 1103 1 0.26688803764312397 1103 2 0.2447319662904377 1103 3 0.41488184123399563 1103 4 0.28882002386486505 1103 5 0.34007409919256304 1103 6 0.3844376225397568 1103 7 0.45184896679995035 1103 8 0.604381239656 1103 9 0.5714320340774321 1103 10-11 0.5707104900433606 1103 12-13 0.44816166480963915 1103 14-15 0.5905993553986519 1103 16-17 0.5939891741831858 1103 18-19 0.5442314757057218 1103 20-21 0.4700480557468154 1103 22-23 0.5503012755987484 1103 24-25 0.5644783692819715 1103 26-27 0.8237552953345002 1103 28-29 0.6829113923012713 1103 30-31 0.5513431754525087 1103 32-33 0.6941055145724846 1103 34-35 0.7924571691336624 1103 36-37 0.9167861584910071 1103 38-39 0.97286576370049 1103 40-41 0.8421974480123708 1103 42-43 0.5611938891174901 1103 44-45 0.8343311957853743 1103 46-47 0.8039757544255295 1103 48-49 0.940767228330408 1103 50-51 0.9416405359144022 1103 52-53 0.9072970200792803 1103 54-55 0.8011674338401562 1103 56-57 0.8132146249119252 1103 58-59 0.8704254729469234 1103 60-61 0.9654174869046628 1103 62-63 0.6441036705794296 1103 64-65 0.8349627141235416 1103 66-67 0.48934908293595925 1103 68-69 -6.619920216222987 1103 70-71 -7.710236708755712 1103 72-73 -0.9226328050084653 1103 74-75 0.546710207059963 1103 76-77 0.8972591555286584 1103 78-79 0.6585547038280559 1103 80-81 0.43453989820548955 1103 82-83 0.3461077211682415 1103 84-85 0.06822032938426403 1103 86-87 0.09663621988684312 1103 88-89 0.2033734107797649 1103 90-91 -0.09257589284654344 1103 92-93 -0.05883330435411338 1103 94-95 0.1776727113250267 1103 96-97 0.34968872181720556 1103 98-99 0.16133448499513037 1103 100-101 0.193341335709718 1104 1 0.2750460126743377 1104 2 0.22574584638544692 1104 3 0.35053365149402893 1104 4 0.2778798153960125 1104 5 0.46474230180965037 1104 6 0.3829573643248594 1104 7 0.3821729171842989 1104 8 0.6316562452464467 1104 9 0.5085584993726115 1104 10-11 0.47114758900213616 1104 12-13 0.4060821668952954 1104 14-15 0.6365381707132656 1104 16-17 0.6050759567405919 1104 18-19 0.5126859557280952 1104 20-21 0.43666290998009316 1104 22-23 0.3843496086272964 1104 24-25 0.4110686561189283 1104 26-27 0.545774543785086 1104 28-29 0.3010292230964495 1104 30-31 0.4299362781419873 1104 32-33 0.48951903178519984 1104 34-35 0.15890372422233412 1104 36-37 0.523180481756782 1104 38-39 0.722016372587845 1104 40-41 0.6684149553003209 1104 42-43 0.5078013165907009 1104 44-45 0.49384888425978346 1104 46-47 0.4818705990417058 1104 48-49 0.6777833873914361 1104 50-51 0.6855896111605233 1104 52-53 0.6082601557409255 1104 54-55 0.48144923303136267 1104 56-57 0.5187317449182842 1104 58-59 0.48942695065823827 1104 60-61 0.552980669123631 1104 62-63 0.14865574474922738 1104 64-65 0.152865102657195 1104 66-67 -0.01564322287947917 1104 68-69 -6.687113038447844 1104 70-71 -4.465844727619071 1104 72-73 0.08131960158270246 1104 74-75 0.7609402715831273 1104 76-77 0.8913597713181147 1104 78-79 0.24917798626244547 1104 80-81 0.2693637622499345 1104 82-83 0.17922707246265546 1104 84-85 0.31343623263466824 1104 86-87 0.2209968843424619 1104 88-89 0.014820808892309856 1104 90-91 -0.0061920526207082105 1104 92-93 -0.02568871328181288 1104 94-95 0.18151012538625722 1104 96-97 0.13167141716060726 1104 98-99 -0.0796396838368274 1104 100-101 0.016825000657902933 1105 1 0.2643016123064612 1105 2 0.2366005696107969 1105 3 0.2561839499509766 1105 4 0.24652346932889913 1105 5 0.5080331903120978 1105 6 0.3597497185274747 1105 7 0.3572616175088186 1105 8 0.49020739981374817 1105 9 0.44013516740814396 1105 10-11 0.5028934270536567 1105 12-13 0.36068107357834833 1105 14-15 0.46222456939915446 1105 16-17 0.4280635545089311 1105 18-19 0.6444413318505937 1105 20-21 0.7594505320045215 1105 22-23 0.5667104281244164 1105 24-25 0.5214141758529038 1105 26-27 0.752267429953065 1105 28-29 0.670274970872498 1105 30-31 0.6351211037932742 1105 32-33 0.8363660020281642 1105 34-35 0.8374112128532332 1105 36-37 0.7769134660747454 1105 38-39 0.6435460650012459 1105 40-41 0.8768991316903865 1105 42-43 0.7578790780178934 1105 44-45 0.7649161296524412 1105 46-47 0.5864444651755107 1105 48-49 0.6821063997682657 1105 50-51 0.5608609045304149 1105 52-53 0.5233015940521462 1105 54-55 0.6609498764153443 1105 56-57 0.7097002376565236 1105 58-59 0.6393516048782608 1105 60-61 0.809610935888287 1105 62-63 0.5401102557462742 1105 64-65 0.3764484438559066 1105 66-67 0.6455104553667717 1105 68-69 -2.192488476097129 1105 70-71 -2.3857784741359964 1105 72-73 1.2027558059584145 1105 74-75 1.388328805028756 1105 76-77 1.2913887092775695 1105 78-79 0.8654552896131982 1105 80-81 0.6770789410065845 1105 82-83 0.5143738429540043 1105 84-85 0.6087868339440483 1105 86-87 0.6491120793362448 1105 88-89 0.6190229891010866 1105 90-91 0.7400894071374715 1105 92-93 0.6263297093902089 1105 94-95 0.47856749352117944 1105 96-97 0.45114536888800316 1105 98-99 0.4611086737098411 1105 100-101 0.49356269150337084 1106 1 0.10040908351651723 1106 2 0.13778401491914494 1106 3 0.04701675683184092 1106 4 0.03930160799635729 1106 5 0.153661848970728 1106 6 0.09410415882230438 1106 7 0.21715705127464702 1106 8 0.33126786483312287 1106 9 0.373778593027545 1106 10-11 0.3234197654272961 1106 12-13 0.09648564549249983 1106 14-15 0.20320189337365946 1106 16-17 0.3299165679873539 1106 18-19 0.2621508952782534 1106 20-21 0.3126583203758315 1106 22-23 0.3825042750422014 1106 24-25 0.18581195019292807 1106 26-27 0.3889936471372195 1106 28-29 0.42150367001030276 1106 30-31 0.21149875363503412 1106 32-33 0.4520244471070711 1106 34-35 0.28541122674314323 1106 36-37 0.3904563039276354 1106 38-39 0.3632772803214195 1106 40-41 0.3687644799067584 1106 42-43 0.28713396112807743 1106 44-45 0.341353927773973 1106 46-47 0.3764404048565808 1106 48-49 0.1583624632729439 1106 50-51 0.17299205605621637 1106 52-53 -0.09056914493830703 1106 54-55 -0.15784096705700534 1106 56-57 0.00506686064438 1106 58-59 0.30798530631907184 1106 60-61 0.5555438580306209 1106 62-63 0.06209079349918056 1106 64-65 0.30059749451762485 1106 66-67 0.25291283594887304 1106 68-69 -3.208526115774454 1106 70-71 -3.2165130297319813 1106 72-73 0.5877704015275818 1106 74-75 1.0031062328303584 1106 76-77 1.0195853299115782 1106 78-79 0.7517306427332073 1106 80-81 0.48380962032497976 1106 82-83 0.2908708964323985 1106 84-85 0.012459177864222681 1106 86-87 0.11397692677168436 1106 88-89 0.2818846879911092 1106 90-91 0.32930207979714154 1106 92-93 0.3933456577375942 1106 94-95 0.5958660643732507 1106 96-97 0.494357381280782 1106 98-99 0.5640940622664878 1106 100-101 0.6889431547964264 1107 1 0.0024379560124643262 1107 2 -0.008337107484766904 1107 3 0.14096291487201995 1107 4 -0.1668453309609106 1107 5 0.31675194439256416 1107 6 0.1376324418247492 1107 7 0.038290691030688606 1107 8 0.0499381104010439 1107 9 0.16406906290848866 1107 10-11 0.01928707165686916 1107 12-13 0.2548663946074825 1107 14-15 0.14013165484755064 1107 16-17 0.0051717951145136 1107 18-19 0.2992948109131106 1107 20-21 0.30452851375144974 1107 22-23 0.24494077569279682 1107 24-25 0.18564949645017492 1107 26-27 0.5262811692917708 1107 28-29 0.43917138828988556 1107 30-31 0.24635731402822358 1107 32-33 0.2245211190706371 1107 34-35 0.18160496791174552 1107 36-37 0.3517429945261341 1107 38-39 0.40782675661197487 1107 40-41 0.242545170719616 1107 42-43 -0.08753291161536936 1107 44-45 0.3825709664920751 1107 46-47 0.3260599394855035 1107 48-49 0.10537095850340705 1107 50-51 0.328625459963078 1107 52-53 0.06271353389413648 1107 54-55 0.01153291680164159 1107 56-57 0.24301719406935618 1107 58-59 0.49590286421603125 1107 60-61 0.4530624709578426 1107 62-63 0.18463217583943603 1107 64-65 0.17306017416816388 1107 66-67 0.24445190846874 1107 68-69 -2.6324635906297225 1107 70-71 -2.608990269380115 1107 72-73 0.9281337749526877 1107 74-75 1.133780399034908 1107 76-77 0.8380587410880835 1107 78-79 0.672709647237383 1107 80-81 0.3472293868161671 1107 82-83 0.3584029820174308 1107 84-85 0.290624126275123 1107 86-87 0.17611049973399773 1107 88-89 0.19543923283843867 1107 90-91 0.2256813159564679 1107 92-93 0.22019728090290513 1107 94-95 0.6009871759037644 1107 96-97 0.40056862862003584 1107 98-99 0.49769229228442313 1107 100-101 0.7295646160879059 1108 1 -0.005909324520331438 1108 2 0.11210883821951612 1108 3 -0.015337940684787554 1108 4 0.020617127315837536 1108 5 -0.009657757177691906 1108 6 -0.06417187519728174 1108 7 0.19869365376300152 1108 8 0.2804603335232798 1108 9 0.38897067860958146 1108 10-11 0.3147765946889436 1108 12-13 0.17996572566788416 1108 14-15 0.27911571664373014 1108 16-17 0.3468543375688782 1108 18-19 0.23994128070247456 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0.8083471491004488 2311 64-65 1.0366682741701396 2311 66-67 1.1487177742273786 2311 68-69 3.2611421430337977 2311 70-71 4.541774910331199 2311 72-73 3.527143252392637 2311 74-75 2.422857266231791 2311 76-77 2.1682505798920886 2311 78-79 1.5597123742516175 2311 80-81 1.2513064861941139 2311 82-83 1.100045259071134 2311 84-85 1.257220746355035 2311 86-87 1.1121562273162802 2311 88-89 1.3165657053593343 2311 90-91 1.409819819272844 2311 92-93 1.4642518555376647 2311 94-95 1.7104064751749704 2311 96-97 1.6709377456132088 2311 98-99 1.9446898095373832 2311 100-101 2.084986743515234 2312 1 -0.053223466848475454 2312 2 0.019837273599655703 2312 3 -0.025114588267538096 2312 4 0.06092911986967664 2312 5 -1.2268743467767749 2312 6 -0.16192798754517668 2312 7 0.1573385122140678 2312 8 0.17627303435617847 2312 9 -2.262271999753999 2312 10-11 0.06417065499014285 2312 12-13 -0.9387068032737531 2312 14-15 -3.8346122990154896 2312 16-17 -0.75700570234347 2312 18-19 -0.03321202819463309 2312 20-21 0.0927053960325992 2312 22-23 0.40042672295085424 2312 24-25 0.43265850924807125 2312 26-27 0.5591092129967521 2312 28-29 0.43245244247572856 2312 30-31 0.2438855047356583 2312 32-33 0.6384567684381253 2312 34-35 0.4892887356674649 2312 36-37 0.6597776155295278 2312 38-39 0.5266256009676624 2312 40-41 0.17982407113346 2312 42-43 -0.06919793149759812 2312 44-45 0.7064548809237223 2312 46-47 0.9399448792118861 2312 48-49 0.906049089750276 2312 50-51 0.9106087130469618 2312 52-53 1.0060468977033246 2312 54-55 1.178776121285246 2312 56-57 1.1333877173027318 2312 58-59 1.0920364908141345 2312 60-61 0.8695892752316929 2312 62-63 1.0614479107831158 2312 64-65 1.1968370530534287 2312 66-67 1.6891893383225813 2312 68-69 3.9411848978442023 2312 70-71 4.685393237872372 2312 72-73 2.8868380701539813 2312 74-75 2.4962041304938865 2312 76-77 2.2120305892373615 2312 78-79 1.6052970079001554 2312 80-81 1.4750346617943393 2312 82-83 1.5447894841754497 2312 84-85 1.4724341894450355 2312 86-87 1.3240825712277342 2312 88-89 1.5260143382903273 2312 90-91 1.7144545018215105 2312 92-93 1.8023405322104793 2312 94-95 1.7041638263507473 2312 96-97 1.855792962456004 2312 98-99 2.469336688722141 2312 100-101 2.345885283874779 2313 1 -0.33290499826641806 2313 2 -0.2697632726671877 2313 3 -0.13962717879072528 2313 4 -0.06874377869251447 2313 5 0.017109644050179895 2313 6 0.10847020341359581 2313 7 -2.8093866032382024 2313 8 -3.0475646383350394 2313 9 -0.8969930727692486 2313 10-11 -0.05258881649979941 2313 12-13 -0.05682745980730175 2313 14-15 -0.5733824736875874 2313 16-17 -0.07267630374756351 2313 18-19 0.25901656982569676 2313 20-21 -2.522435463721891 2313 22-23 -0.6376763248954305 2313 24-25 0.00876366093884684 2313 26-27 -1.2311638385793984 2313 28-29 -1.4163786532655607 2313 30-31 -0.6880591405358203 2313 32-33 -0.842751862546848 2313 34-35 -1.1277197829929477 2313 36-37 -1.9124456741614395 2313 38-39 -1.8022347568417985 2313 40-41 -0.5861411581399949 2313 42-43 0.02057090673827844 2313 44-45 -0.05957660503045048 2313 46-47 -0.0463998137643955 2313 48-49 -1.453947620800399 2313 50-51 -0.3101069058742354 2313 52-53 -0.06115413558909566 2313 54-55 0.01510516599253009 2313 56-57 -0.09677785814238504 2313 58-59 -0.048514780359258936 2313 60-61 -1.231555223668579E-5 2313 62-63 0.5244164673111698 2313 64-65 0.39178155139097015 2313 66-67 0.9218235597422719 2313 68-69 1.4872167267741325 2313 70-71 4.049371585189114 2313 72-73 3.266703210526554 2313 74-75 1.8594723620913847 2313 76-77 1.9434967443504902 2313 78-79 1.2558358890742518 2313 80-81 1.1715137817808987 2313 82-83 0.9259168143157304 2313 84-85 0.9122953388269615 2313 86-87 0.9800161103205376 2313 88-89 0.4485731401201072 2313 90-91 0.8329726984393808 2313 92-93 0.8825307980127981 2313 94-95 1.2075931941957556 2313 96-97 1.155921305544009 2313 98-99 1.4377394623047444 2313 100-101 1.4462637654197579 2314 1 -0.28680397468167484 2314 2 -0.18513942235633252 2314 3 -0.160367651174127 2314 4 2.0177213911409808E-4 2314 5 -0.13941122416000695 2314 6 -2.422190586401186 2314 7 -0.6414753569514957 2314 8 -0.10913621630185588 2314 9 -0.27647763801275005 2314 10-11 -0.2884352525759297 2314 12-13 -2.171028969421677 2314 14-15 -0.6159760871103401 2314 16-17 -1.8011358562466313 2314 18-19 -0.4768241933617148 2314 20-21 -0.728160230028216 2314 22-23 -0.49289012042183344 2314 24-25 -0.3841580215069271 2314 26-27 -2.103314141179105 2314 28-29 -0.9631899616410564 2314 30-31 -0.4301355821244286 2314 32-33 -0.0060682645895582255 2314 34-35 -0.45198131959462273 2314 36-37 -0.6323542271953215 2314 38-39 -0.5916260245837535 2314 40-41 -0.7275934645996074 2314 42-43 -0.762327446441688 2314 44-45 -3.6205597675055046 2314 46-47 -4.401701245640634 2314 48-49 -3.2200070797163605 2314 50-51 -3.848212090620656 2314 52-53 -1.317479308700232 2314 54-55 -1.5741868848819394 2314 56-57 -1.1484725456190326 2314 58-59 -1.7846145402591347 2314 60-61 -1.3164699134477154 2314 62-63 -1.0616520134540224 2314 64-65 -1.133679155638756 2314 66-67 -0.9751260693094892 2314 68-69 -1.0564518012520914 2314 70-71 1.172164000106541 2314 72-73 0.9538878028043065 2314 74-75 0.634037046129091 2314 76-77 0.6787833249952939 2314 78-79 -1.269095869986817 2314 80-81 -0.7805608718696817 2314 82-83 0.3641075618733538 2314 84-85 0.579729866844577 2314 86-87 0.26871372070419497 2314 88-89 0.4043081763111793 2314 90-91 0.8777902798560895 2314 92-93 0.08281389472146472 2314 94-95 0.6263601664029075 2314 96-97 0.5876089664633248 2314 98-99 0.12696179533304885 2314 100-101 0.3171406023109071 2315 1 -0.2833581580512359 2315 2 -0.35179125777633047 2315 3 -0.43175562168478265 2315 4 -0.11153669834067159 2315 5 -0.26041120500001824 2315 6 -0.21226784371337715 2315 7 -1.0175556074789327 2315 8 -2.838107176153173 2315 9 -0.6633866855699608 2315 10-11 -1.618259470369587 2315 12-13 -0.6706138364407437 2315 14-15 -0.46703659221009275 2315 16-17 -0.3394956339056634 2315 18-19 -0.3206151877435772 2315 20-21 -0.6173895516157728 2315 22-23 -0.25764043390045543 2315 24-25 -5.329207613931498 2315 26-27 -0.8552387615453156 2315 28-29 -0.9586786370262175 2315 30-31 -1.193432717281091 2315 32-33 -0.42542325438154904 2315 34-35 -2.37830224080664 2315 36-37 -3.6199334484058454 2315 38-39 -1.2286041545267707 2315 40-41 -0.3358216131703813 2315 42-43 -1.51193264433239 2315 44-45 -4.833420374366057 2315 46-47 -3.276948888430411 2315 48-49 -2.460150226083737 2315 50-51 -1.2763270249764247 2315 52-53 -0.5083670333244292 2315 54-55 -1.5588158943746464 2315 56-57 -1.7329225157081716 2315 58-59 -1.143742675843697 2315 60-61 -1.8363769236599836 2315 62-63 -0.7735552597939304 2315 64-65 -0.1948863623473045 2315 66-67 0.06506008057693435 2315 68-69 2.7790271664079818 2315 70-71 3.1241650475189857 2315 72-73 -2.590142904262965 2315 74-75 -2.426325205296731 2315 76-77 -0.8117128972945764 2315 78-79 -0.5333918789622061 2315 80-81 -0.9515347738429476 2315 82-83 -1.4780429121233531 2315 84-85 -0.8737147788979698 2315 86-87 -0.43207418040474366 2315 88-89 -0.5063637217406765 2315 90-91 -0.37782625648720725 2315 92-93 -1.6316461450035682 2315 94-95 -0.5205656142067632 2315 96-97 -0.627261522691061 2315 98-99 -3.3484357211072826 2315 100-101 -4.9318123045779565 2316 1 -0.6034970232888348 2316 2 -0.41667692554874236 2316 3 -1.1120331705180746 2316 4 -0.3964002512109559 2316 5 -0.2074044638147612 2316 6 -0.45574383405911334 2316 7 -0.4554025151389993 2316 8 -0.8218379646833966 2316 9 -5.0621106511760665 2316 10-11 -1.5709462947468396 2316 12-13 -0.8002959403989465 2316 14-15 -0.4708808161109914 2316 16-17 -0.45616400346521147 2316 18-19 -0.4905700270245532 2316 20-21 -3.4204547587897167 2316 22-23 -1.0052848789503699 2316 24-25 -0.5549803299548088 2316 26-27 -0.6752625112246093 2316 28-29 -0.09873544553251179 2316 30-31 -0.009821358304456851 2316 32-33 -1.2193253085244535 2316 34-35 -0.7053782375098407 2316 36-37 -0.49157664920870303 2316 38-39 -5.282542037535755 2316 40-41 -4.850896302051854 2316 42-43 -3.5795252306179677 2316 44-45 -2.5011669021674834 2316 46-47 -2.6488207309832106 2316 48-49 -2.9599342992672604 2316 50-51 -1.4205949740349482 2316 52-53 -1.1005389483069372 2316 54-55 -0.3492967232066633 2316 56-57 -3.7496692183399354 2316 58-59 -0.8847401623738129 2316 60-61 -0.6381154637585702 2316 62-63 -0.545653019468066 2316 64-65 -0.04881357389427876 2316 66-67 -0.06624272329294456 2316 68-69 2.41879584543992 2316 70-71 3.4421882386494147 2316 72-73 2.425857477185044 2316 74-75 1.008276215597082 2316 76-77 -1.4893804789823477 2316 78-79 -0.05998993039865397 2316 80-81 -0.02431142014301102 2316 82-83 0.1223482991329945 2316 84-85 0.15125258232193417 2316 86-87 0.11052924763214023 2316 88-89 0.003063493212085433 2316 90-91 0.04138964081246144 2316 92-93 0.022057646998280944 2316 94-95 0.10189259112578597 2316 96-97 0.46396517321995034 2316 98-99 0.4242382975155543 2316 100-101 0.4217597970416058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 26.0 4 76.0 5 236.0 6 650.0 7 1344.0 8 2706.0 9 4536.0 10 7415.0 11 10643.0 12 13625.0 13 16786.0 14 20293.0 15 25469.0 16 30220.0 17 33852.0 18 28795.0 19 22993.0 20 22164.0 21 22492.0 22 23359.0 23 25984.0 24 30541.0 25 36343.0 26 44855.0 27 54151.0 28 65714.0 29 77954.0 30 90493.0 31 105630.0 32 120623.0 33 139563.0 34 162293.0 35 181322.0 36 179972.0 37 148890.0 38 64392.0 39 3926.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.158764914517114 22.183955097790882 16.123475580440175 20.533804407251825 2 15.610321093230167 23.48322885536876 38.25728667182051 22.64916337958056 3 17.77548148074901 28.592468804969673 31.787892471771478 21.844157242509844 4 11.896600554887991 19.476953450451912 38.19383683873327 30.432609155926826 5 11.746819671806941 40.25773334629801 33.851590727180934 14.143856254714116 6 27.411663043699196 39.379816088392715 17.880044892934308 15.328475974973783 7 25.392799441420273 36.358112892655235 21.49284937739345 16.75623828853104 8 26.0038970934407 33.906800466508564 20.473529264732623 19.615773175318115 9 26.6090068234843 16.98542737557071 21.813450410941744 34.59211539000325 10-11 23.626844851447668 28.333259162929302 27.112304430500405 20.92759155512262 12-13 25.21824327553615 26.741565132934614 28.144414394964436 19.8957771965648 14-15 22.639783643743915 27.6172575207476 27.93288693100321 21.81007190450528 16-17 19.48728794937154 30.9655445196449 28.501719477893992 21.045448053089565 18-19 18.971713072667928 28.26926568473767 33.15852056373341 19.600500678860985 20-21 20.375909655991137 27.528729665099373 32.857595679027604 19.23776499988189 22-23 20.052391628106786 27.233236409462247 32.803850292996486 19.91052166943448 24-25 19.7832241094301 28.086896043272773 32.92527964304138 19.20460020425575 26-27 19.839303220610457 27.825357963873852 33.202060913808765 19.133277901706926 28-29 19.05206230039452 27.746929849017434 33.88434768922537 19.31666016136267 30-31 19.418703502529077 27.96375926859315 33.294997740791146 19.32253948808662 32-33 19.433627521292255 28.23193595197332 32.90536863955076 19.429067887183667 34-35 19.82899798985975 27.926995485836997 32.67639818466591 19.567608339637342 36-37 20.321144348908536 27.44382668058102 32.61464217653262 19.62038679397783 38-39 19.54290484351384 28.17325535260562 32.73364866934454 19.550191134536004 40-41 19.08396526879409 28.05969377909409 33.36006219201565 19.49627876009617 42-43 19.482345269745167 28.100632361062967 32.342616924118595 20.07440544507327 44-45 19.471803031369305 28.258711008286564 31.917317994686744 20.352167965657383 46-47 20.008703213014027 28.518141910753858 30.986654615218196 20.48650026101392 48-49 20.05508649872248 28.422846603067963 31.01262673811785 20.509440160091703 50-51 20.04531707936564 28.798226020239028 30.633037211902842 20.5234196884925 52-53 19.907125312392555 29.129099168849603 30.55250709711358 20.411268421644266 54-55 19.825722669958594 28.849032015517214 30.348428244711577 20.976817069812615 56-57 20.09070769531732 29.37979774167677 29.791188764269073 20.73830579873684 58-59 19.902897150763273 28.44195313435821 30.50577331166212 21.149376403216394 60-61 20.249768897838422 28.942067186514898 30.37038156237423 20.43778235327245 62-63 20.067593606624875 28.706461963076773 30.501752330191252 20.724192100107096 64-65 20.06680538673369 28.538700838500997 30.689109101272514 20.705384673492794 66-67 19.823558652896807 28.87777988379377 30.222231147573 21.076430315736424 68-69 19.8483969950504 28.705672954457683 30.335733073367642 21.110196977124275 70-71 19.72040190044918 28.83359211271477 30.5605427384954 20.885463248340653 72-73 20.060971660030624 28.519135646783344 29.854751282761587 21.565141410424445 74-75 19.86203469826514 29.35757175352684 29.33179360663482 21.4485999415732 76-77 19.15800221266946 29.296459178450142 30.083690644591343 21.461847964289056 78-79 19.61799355410824 29.002749338763152 29.721621208807992 21.657635898320617 80-81 19.666368634366556 29.945653741404104 29.09317148943359 21.294806134795753 82-83 19.783245175054407 29.50164134536758 28.86636336592951 21.848750113648503 84-85 19.459421153293103 29.027347566494257 28.913631702884068 22.599599577328572 86-87 19.636756779325115 28.979525577028564 29.221272040720862 22.16244560292546 88-89 19.82434455385714 28.723881724417343 29.24969442269924 22.202079299026273 90-91 19.528087449406055 28.88338293725733 29.476789621220135 22.111739992116476 92-93 19.495766787950437 29.029462452664447 29.06346878916016 22.411301970224955 94-95 19.364625416137862 28.76291570148522 29.114710423574806 22.75774845880211 96-97 19.56491633386878 28.511661161098722 28.86732945974359 23.056093045288904 98-99 19.4058150414846 28.69832693523831 28.673602652201314 23.222255371075775 100-101 19.2970469963117 28.417969769444074 29.12129088902185 23.16369234522238 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8460.0 1 5606.0 2 2782.0 3 2836.5 4 2525.0 5 2603.5 6 4371.5 7 7721.0 8 10560.0 9 11248.5 10 9779.5 11 8045.0 12 7303.0 13 6775.5 14 6503.0 15 6318.0 16 6256.5 17 6498.0 18 6727.0 19 7004.5 20 7482.5 21 8122.5 22 8992.5 23 9958.5 24 10877.0 25 12450.5 26 15187.0 27 17993.0 28 19401.0 29 21503.5 30 25499.5 31 29385.0 32 34698.5 33 41929.0 34 49704.5 35 55574.5 36 62278.5 37 68687.0 38 67835.5 39 63296.5 40 58671.0 41 55009.0 42 52877.0 43 53427.5 44 54129.0 45 53223.5 46 51709.0 47 52694.5 48 61671.0 49 60848.0 50 50057.5 51 44397.5 52 41411.0 53 40717.0 54 39879.0 55 37599.5 56 34129.5 57 30727.0 58 28943.5 59 27903.0 60 25715.5 61 22571.5 62 19466.5 63 16300.5 64 13145.0 65 10085.5 66 7349.0 67 5615.5 68 3899.0 69 2225.0 70 1598.5 71 1374.5 72 1181.5 73 930.5 74 736.0 75 627.5 76 406.5 77 208.5 78 118.0 79 74.5 80 46.0 81 31.0 82 24.0 83 13.5 84 5.5 85 2.5 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020655584066853774 2 0.018128571122504645 3 4.944155760683084E-4 4 0.1069036345587698 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010877142673502786 12-13 0.0 14-15 0.0 16-17 6.042857040834881E-4 18-19 1.3733766001897455E-4 20-21 0.0 22-23 1.0987012801517965E-4 24-25 0.003515844096485749 26-27 0.003872922012535083 28-29 0.0011811038761631813 30-31 1.922727240265644E-4 32-33 5.218831080721034E-4 34-35 0.0032961038404553896 36-37 0.08707207645202988 38-39 0.1014925307540222 40-41 0.07954597268299007 42-43 0.08308928431147962 44-45 0.08146869992325571 46-47 0.08034253111110012 48-49 0.07545331041442463 50-51 0.07526103769039807 52-53 0.060730713260390555 54-55 0.051199479655073724 56-57 0.029088116392018814 58-59 0.02224870092307388 60-61 0.013651363405886073 62-63 0.0022248700923073877 64-65 0.006509805084899395 66-67 0.15307655585714905 68-69 0.7093764815300074 70-71 0.8627551802391984 72-73 0.35012863045237375 74-75 0.052765128979290025 76-77 0.1934263603707238 78-79 0.014118311449950587 80-81 0.003213701244444005 82-83 0.0014008441321935404 84-85 2.472077880341542E-4 86-87 0.0019501947722694389 88-89 3.2961038404553895E-4 90-91 0.004312402524595802 92-93 0.004504675248622365 94-95 0.07539837535041703 96-97 0.03614727211699411 98-99 0.014173246513958177 100-101 0.013843636129912636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1820331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.72734270217601 #Duplication Level Percentage of deduplicated Percentage of total 1 67.87843038974941 16.784532103331326 2 11.488611519095121 5.68165668409664 3 5.332607201555633 3.9558361730687372 4 3.251405355169924 3.2159445792390806 5 2.0955578152641543 2.590878812513 6 1.5352544261658279 2.2777657398501003 7 1.1524503659117837 1.9947924601604172 8 0.9183221556970523 1.816613332393764 9 0.7027800713186291 1.5640095300979893 >10 4.711582633467557 22.312812122685024 >50 0.5228016522772346 9.027732711441192 >100 0.3781319304676106 17.851967470923384 >500 0.023819330867518043 3.94463893493222 >1k 0.007329024882313244 3.345682675588007 >5k 0.0 0.0 >10k+ 9.161281102891555E-4 3.635136669679183 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21841 1.1998367329897694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17615 0.9676811524936948 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13977 0.767827389634083 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10754 0.590771678337621 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.2589638917317785 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4397 0.24154947644137248 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3808 0.2091927237409021 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3610 0.19831558106739927 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3350 0.18403246442542592 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2542 0.13964493270729336 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2495 0.13706298469893663 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2274 0.12492233555325928 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2237 0.12288973818497845 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2197 0.12069233562467487 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.4935064007589825E-5 0.0 12-13 0.0 0.0 0.0 5.4935064007589825E-5 0.0 14-15 0.0 0.0 0.0 1.0987012801517965E-4 0.0 16-17 0.0 0.0 0.0 2.472077880341542E-4 0.0 18-19 0.0 0.0 0.0 3.845454480531288E-4 0.0 20-21 0.0 0.0 0.0 6.866883000948729E-4 0.0 22-23 0.0 0.0 0.0 0.0016755194522314898 0.0 24-25 0.0 0.0 0.0 0.002801688264387081 0.0 26-27 0.0 0.0 0.0 0.005685779124785547 0.0 28-29 0.0 0.0 0.0 0.012992142637794994 0.0 30-31 0.0 0.0 0.0 0.02939025924406056 0.0 32-33 0.0 0.0 0.0 0.0540011679194608 0.0 34-35 0.0 0.0 0.0 0.09701532303740364 0.0 36-37 0.0 0.0 0.0 0.1616189583103293 0.0 38-39 0.0 0.0 0.0 0.2519047360068032 0.0 40-41 0.0 0.0 0.0 0.3502110330483852 0.0 42-43 0.0 0.0 0.0 0.44744609634181914 0.0 44-45 0.0 0.0 0.0 0.5615462242855832 0.0 46-47 0.0 0.0 0.0 0.6977027804283946 0.0 48-49 0.0 0.0 0.0 0.8474832324450883 0.0 50-51 0.0 0.0 0.0 1.0088000479033759 0.0 52-53 0.0 0.0 0.0 1.1577290064279517 0.0 54-55 0.0 0.0 0.0 1.3217376400226113 0.0 56-57 0.0 0.0 0.0 1.5146421172852629 0.0 58-59 0.0 2.7467532003794913E-5 0.0 1.7022178933391783 0.0 60-61 0.0 5.4935064007589825E-5 0.0 1.903170357478942 0.0 62-63 0.0 5.4935064007589825E-5 0.0 2.086845743988319 0.0 64-65 0.0 5.4935064007589825E-5 0.0 2.2849141172676837 0.0 66-67 0.0 5.4935064007589825E-5 0.0 2.494491386456639 0.0 68-69 0.0 5.4935064007589825E-5 0.0 2.7297507980691424 0.0 70-71 0.0 5.4935064007589825E-5 0.0 3.019917806157232 0.0 72-73 0.0 5.4935064007589825E-5 0.0 3.3477153330905205 0.0 74-75 0.0 5.4935064007589825E-5 0.0 3.69680019732675 0.0 76-77 0.0 5.4935064007589825E-5 0.0 4.087196229696687 2.7467532003794913E-5 78-79 0.0 5.4935064007589825E-5 0.0 4.522721417148859 5.4935064007589825E-5 80-81 0.0 5.4935064007589825E-5 0.0 5.004364590835403 5.4935064007589825E-5 82-83 0.0 5.4935064007589825E-5 0.0 5.489386270958414 5.4935064007589825E-5 84-85 0.0 5.4935064007589825E-5 0.0 5.983087691194623 5.4935064007589825E-5 86-87 0.0 5.4935064007589825E-5 0.0 6.497060150049634 5.4935064007589825E-5 88-89 0.0 5.4935064007589825E-5 0.0 7.037621179884318 5.4935064007589825E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCAT 440 0.0 30.17138 5 CATATAG 330 0.0 28.758348 3 GGTATCA 17165 0.0 26.272831 1 TTAGGCA 565 0.0 26.040228 4 GTACATG 13290 0.0 25.753565 1 CATGGGT 1045 0.0 25.43333 4 GTATCAA 23595 0.0 24.786648 1 TACATGG 13610 0.0 24.620111 2 ACATGGG 13400 0.0 24.2214 3 CATTCCG 495 0.0 23.94554 9 GAGTACT 14920 0.0 22.749632 12-13 CATGGGG 4275 0.0 21.981665 4 GTGTACG 195 4.2492684E-7 21.909624 1 CAGCGTA 195 4.300182E-7 21.882538 9 CATGGGA 8075 0.0 21.864115 4 AGTACTT 15445 0.0 21.700098 12-13 ATGGGTA 765 0.0 21.691841 5 GTGGTAC 425 0.0 21.222265 1 TAGATAT 380 0.0 21.210707 7 ATGGGAT 2775 0.0 21.015121 5 >>END_MODULE