##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939409_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 731377 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.969391982520644 31.0 31.0 31.0 30.0 34.0 2 31.157552124280638 31.0 31.0 34.0 28.0 34.0 3 31.140019442777117 31.0 31.0 34.0 28.0 34.0 4 35.00880530834303 35.0 35.0 37.0 32.0 37.0 5 34.69741186829774 35.0 35.0 37.0 32.0 37.0 6 34.720821136021506 35.0 35.0 37.0 32.0 37.0 7 34.61502480936644 35.0 35.0 37.0 30.0 37.0 8 34.687463510610804 35.0 35.0 37.0 31.0 37.0 9 35.96736703505853 37.0 35.0 39.0 30.0 39.0 10-11 35.828500896254596 37.0 35.0 39.0 30.0 39.0 12-13 35.76807173318275 37.0 35.0 39.0 30.0 39.0 14-15 36.388442622614605 38.0 35.0 40.0 30.0 41.0 16-17 36.44829684280474 38.0 35.0 40.0 30.5 41.0 18-19 36.603395376119295 38.0 35.0 40.0 30.0 41.0 20-21 36.55994719549562 38.0 35.0 40.0 30.0 41.0 22-23 36.38118029415746 38.0 35.0 40.0 30.0 41.0 24-25 36.12058897121457 38.0 34.0 40.0 29.5 41.0 26-27 35.52868288174224 38.0 34.0 40.0 28.0 41.0 28-29 35.19417003816089 37.5 33.5 40.0 27.0 41.0 30-31 34.98366232462875 37.0 33.0 40.0 27.0 41.0 32-33 34.71092473512293 37.0 33.0 39.5 26.5 40.5 34-35 34.562635275651274 37.0 33.0 40.0 25.5 41.0 36-37 34.323549961237504 37.0 33.0 40.0 24.5 41.0 38-39 34.11620956086943 37.0 33.0 40.0 24.0 41.0 40-41 33.82453303836462 37.0 33.0 40.0 23.5 41.0 42-43 33.57598270112405 37.0 33.0 40.0 22.5 41.0 44-45 33.36919194888546 37.0 33.0 40.0 22.0 41.0 46-47 32.87444095179367 36.0 31.5 40.0 19.0 41.0 48-49 32.46005069888717 36.0 31.0 40.0 15.5 41.0 50-51 32.17985457568395 36.0 31.0 39.0 13.5 41.0 52-53 31.639302986011316 35.0 30.0 39.0 9.0 40.0 54-55 31.468137499538543 35.0 30.0 39.0 8.0 40.0 56-57 31.226306679045145 35.0 29.5 39.0 8.0 40.0 58-59 30.905982824179596 35.0 28.5 39.0 6.5 40.0 60-61 30.60699406735514 35.0 28.0 38.5 2.0 40.0 62-63 30.11938644502083 35.0 28.0 38.0 2.0 40.0 64-65 29.553622140154804 34.0 27.0 38.0 2.0 40.0 66-67 27.91182112645052 33.0 23.0 36.5 2.0 39.5 68-69 24.4996417716171 29.0 13.0 35.0 2.0 38.0 70-71 23.927130604325814 28.5 10.5 35.0 2.0 37.5 72-73 25.643385695749252 31.0 19.5 35.0 2.0 37.0 74-75 26.21146002677142 31.0 22.0 35.0 2.0 37.0 76-77 26.088892595747474 30.5 23.0 34.0 2.0 36.0 78-79 26.834212724764384 32.0 25.0 35.0 2.0 37.0 80-81 26.767043535686796 32.0 25.0 35.0 2.0 36.0 82-83 26.425385266422104 32.0 24.0 35.0 2.0 36.0 84-85 26.114569503826345 32.0 24.0 35.0 2.0 35.5 86-87 25.559978643025417 32.0 20.5 34.5 2.0 35.0 88-89 24.89287740795787 31.0 19.0 34.0 2.0 35.0 90-91 24.349596035970507 31.0 16.5 34.0 2.0 35.0 92-93 23.93123450696426 31.0 9.0 34.0 2.0 35.0 94-95 22.712078039096117 30.0 2.0 34.0 2.0 35.0 96-97 21.86651480700104 29.0 2.0 34.0 2.0 35.0 98-99 21.524527705957393 29.0 2.0 34.0 2.0 35.0 100-101 20.38563080326562 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38470711065200547 1101 2 0.330355356496991 1101 3 0.3863691826531017 1101 4 0.31282348996284526 1101 5 0.16243502317541214 1101 6 0.5500166151283992 1101 7 0.6035616427884918 1101 8 0.47587205398636456 1101 9 0.6502890617467401 1101 10-11 0.8206491361653079 1101 12-13 0.6934330983999075 1101 14-15 0.7050208073858428 1101 16-17 0.695996289758007 1101 18-19 0.6462734010221638 1101 20-21 0.7147426753335466 1101 22-23 0.8377039666069521 1101 24-25 0.8732338853168642 1101 26-27 0.7272626438171486 1101 28-29 0.5766057394141697 1101 30-31 0.5727423814303947 1101 32-33 0.7777875315283111 1101 34-35 1.195013135951271 1101 36-37 1.2320113554112382 1101 38-39 0.8840006327112064 1101 40-41 1.0762042892391506 1101 42-43 1.2874446169431408 1101 44-45 1.167522505803511 1101 46-47 0.7696043725666968 1101 48-49 0.8467957855592303 1101 50-51 0.7345216421484082 1101 52-53 0.8748972185034951 1101 54-55 0.7840962794041424 1101 56-57 0.8563024336537701 1101 58-59 1.124816200737886 1101 60-61 0.8516230804044369 1101 62-63 0.9548051031836273 1101 64-65 0.5380348764315563 1101 66-67 -2.125099733936022 1101 68-69 -6.854646112988636 1101 70-71 -10.07201652133275 1101 72-73 -10.253220711860685 1101 74-75 -8.290455405416278 1101 76-77 -3.5346973274442064 1101 78-79 -1.1200699472667246 1101 80-81 -0.3924498000625469 1101 82-83 -0.38568488043037874 1101 84-85 -0.3963508845248569 1101 86-87 -0.4629504536975233 1101 88-89 -0.25537245884338233 1101 90-91 -0.3667730960398359 1101 92-93 -0.7726198774629616 1101 94-95 -4.140637792375667 1101 96-97 -1.4314593392783337 1101 98-99 -0.9109407394885984 1101 100-101 -0.8549367924122073 1102 1 0.3906665862181562 1102 2 0.30174987377947105 1102 3 0.3613393852752722 1102 4 0.219855671130901 1102 5 0.4866304939739834 1102 6 0.490421859466899 1102 7 0.4378882220495157 1102 8 0.2506038775858883 1102 9 0.5799672500661615 1102 10-11 0.6823893030306252 1102 12-13 0.7935522879112256 1102 14-15 0.5643771840246998 1102 16-17 0.48979843516921306 1102 18-19 0.6325666072200207 1102 20-21 0.9406067992906415 1102 22-23 0.7369888295390083 1102 24-25 0.6282994395481012 1102 26-27 0.8261899382152365 1102 28-29 0.8972255248730434 1102 30-31 0.32899983077485473 1102 32-33 0.5471558271183028 1102 34-35 0.6431656985257632 1102 36-37 1.198638292240794 1102 38-39 1.1629040892070392 1102 40-41 0.9444998792272301 1102 42-43 0.8565745335104822 1102 44-45 0.7163902054459399 1102 46-47 0.7856949565953002 1102 48-49 0.9087743314471979 1102 50-51 0.9568100807658126 1102 52-53 1.0304395307800114 1102 54-55 0.620210701335008 1102 56-57 0.3169698949171753 1102 58-59 0.45497114710260433 1102 60-61 0.4839234379729689 1102 62-63 0.42202798756980187 1102 64-65 0.44745084782607236 1102 66-67 -2.564909030717903 1102 68-69 -7.138317149937386 1102 70-71 -10.361051086291035 1102 72-73 -8.311027624852343 1102 74-75 -6.30595004188827 1102 76-77 -2.891073966299988 1102 78-79 -0.6623822237863912 1102 80-81 -0.16658567610545205 1102 82-83 0.06902310526687927 1102 84-85 0.06789226207824228 1102 86-87 0.05492797300092889 1102 88-89 0.11232718358808569 1102 90-91 3.3059883501351806E-4 1102 92-93 -0.5211300085714221 1102 94-95 -4.004761749467441 1102 96-97 -1.0399217945822663 1102 98-99 -0.2655295356745313 1102 100-101 -0.5194183180379532 1103 1 0.3190722886515758 1103 2 0.19648277192262853 1103 3 0.3792885056340545 1103 4 0.15482598686293159 1103 5 0.3816023366890775 1103 6 0.34263817641203076 1103 7 0.3844282622317934 1103 8 0.2391430213725556 1103 9 0.5229966430762616 1103 10-11 0.5699510362695079 1103 12-13 0.5385899464240893 1103 14-15 0.7446325860807548 1103 16-17 0.7033646899746131 1103 18-19 0.6496249874586013 1103 20-21 0.4998007218980476 1103 22-23 0.659261856938457 1103 24-25 0.726767932090624 1103 26-27 0.7069742702379145 1103 28-29 0.8783361788318231 1103 30-31 0.7639955962246034 1103 32-33 0.7498098085145557 1103 34-35 0.8484494840864443 1103 36-37 0.7416794203985049 1103 38-39 0.7853879251016025 1103 40-41 1.1792021245992785 1103 42-43 0.9315628337096982 1103 44-45 0.7364120639257195 1103 46-47 0.9804637671179108 1103 48-49 1.1334126273340885 1103 50-51 1.068045141428044 1103 52-53 1.267449924161955 1103 54-55 1.0478896729401903 1103 56-57 1.1333657341711039 1103 58-59 1.2710290706601377 1103 60-61 0.8847564920663444 1103 62-63 1.1851420150300491 1103 64-65 0.9555035626747141 1103 66-67 -2.087661785296376 1103 68-69 -6.826272505477011 1103 70-71 -10.79721117632648 1103 72-73 -8.69173419384865 1103 74-75 -6.833705079871972 1103 76-77 -3.3195871230971115 1103 78-79 -0.635743438699361 1103 80-81 0.020040638619896356 1103 82-83 0.43119096877721574 1103 84-85 0.39471188154840675 1103 86-87 0.1922518220727909 1103 88-89 0.4430908023682818 1103 90-91 0.45930494693342183 1103 92-93 0.3691237392529807 1103 94-95 1.1648577913913698 1103 96-97 1.0294484804302115 1103 98-99 0.8591062152405975 1103 100-101 0.4048157514991608 1104 1 0.36991417741814203 1104 2 0.20483765385338515 1104 3 0.46485990616487527 1104 4 0.16353076681121337 1104 5 0.41909262915261536 1104 6 0.39896749018520694 1104 7 0.4427844242772423 1104 8 0.3208399414165868 1104 9 0.6525485175908798 1104 10-11 0.5453771011115904 1104 12-13 0.6615577643405715 1104 14-15 0.6134549658993222 1104 16-17 0.8427115754555601 1104 18-19 0.7342149667207352 1104 20-21 0.7091912166197076 1104 22-23 0.6417008168025973 1104 24-25 0.5870690150676552 1104 26-27 1.0117397105547 1104 28-29 0.8821231848142261 1104 30-31 0.9590936103399983 1104 32-33 0.6506670586575041 1104 34-35 0.7361599562780796 1104 36-37 0.70240976850436 1104 38-39 0.9745739428354625 1104 40-41 1.0701683551735286 1104 42-43 1.172648968360697 1104 44-45 0.9018242181880751 1104 46-47 0.8856798078084864 1104 48-49 0.9308515445277408 1104 50-51 0.9955223767216594 1104 52-53 1.285535811324113 1104 54-55 1.19749881125232 1104 56-57 1.0438558474185875 1104 58-59 1.0267085128714903 1104 60-61 0.9510963027800514 1104 62-63 1.1068224642649653 1104 64-65 1.2443806460613835 1104 66-67 -1.3777379094504347 1104 68-69 -6.012198497779256 1104 70-71 -7.732029869414713 1104 72-73 -6.755368695499996 1104 74-75 -5.42301014827277 1104 76-77 -2.237691785274844 1104 78-79 0.3786002464948375 1104 80-81 0.9301979220458243 1104 82-83 0.9588499803579857 1104 84-85 1.15855632201027 1104 86-87 1.4024039850399248 1104 88-89 1.3600284266460818 1104 90-91 1.1320521584169043 1104 92-93 0.9247873933830597 1104 94-95 1.8558658390457765 1104 96-97 2.1476094676546644 1104 98-99 1.7892247538616566 1104 100-101 1.790789534750136 1105 1 0.40659875380766053 1105 2 0.35278410681759453 1105 3 0.4903190325346678 1105 4 0.4287737622882517 1105 5 0.5089083714954441 1105 6 0.4576688835009364 1105 7 0.6401890322364423 1105 8 0.48965881037205605 1105 9 0.6468295484623852 1105 10-11 0.7664043299614178 1105 12-13 0.9292261612686019 1105 14-15 1.0054183572115818 1105 16-17 1.085911951863288 1105 18-19 0.80167087541151 1105 20-21 1.1350297862703158 1105 22-23 1.0949534046057678 1105 24-25 0.979584875796796 1105 26-27 0.8777537347783593 1105 28-29 1.28528318845688 1105 30-31 1.2749270195619857 1105 32-33 1.1947735456198316 1105 34-35 1.2519766699957202 1105 36-37 1.2151256717311512 1105 38-39 1.0289041495561548 1105 40-41 1.3475550534098844 1105 42-43 1.5151056359380277 1105 44-45 1.309414601577565 1105 46-47 1.365816710944209 1105 48-49 1.137687594674965 1105 50-51 1.033776330520645 1105 52-53 1.204430568935745 1105 54-55 1.182351435482051 1105 56-57 1.1173297265431366 1105 58-59 1.1252544862193936 1105 60-61 1.1980971830884641 1105 62-63 1.2097016798798172 1105 64-65 1.143611135087884 1105 66-67 1.9459606000963596 1105 68-69 -4.941996937508488 1105 70-71 -4.92609385872959 1105 72-73 -1.334810458545256 1105 74-75 0.9411946903879489 1105 76-77 1.3374364665540739 1105 78-79 1.2700961636851353 1105 80-81 1.2525683801097536 1105 82-83 1.4572927451335076 1105 84-85 1.4387979514914981 1105 86-87 1.4358025826175584 1105 88-89 1.0685446516407708 1105 90-91 1.0266428150055589 1105 92-93 1.3283283580796557 1105 94-95 2.408379271337914 1105 96-97 1.9520155630315266 1105 98-99 1.8520878810814025 1105 100-101 1.4493042419717028 1106 1 0.11932344121589367 1106 2 0.14021243095477942 1106 3 0.18715679392938966 1106 4 0.13817083497646365 1106 5 0.16757516180780385 1106 6 0.35355766419984747 1106 7 0.4309697002323034 1106 8 0.3523224477192386 1106 9 0.4543642078957788 1106 10-11 0.43077427787567046 1106 12-13 0.343205852463484 1106 14-15 0.32716531630031653 1106 16-17 0.43585244737340645 1106 18-19 0.2963015611814299 1106 20-21 0.5462204942964632 1106 22-23 0.5045336274771302 1106 24-25 0.6520543894055493 1106 26-27 0.6729470084526454 1106 28-29 0.8705083008971783 1106 30-31 0.6934599634113923 1106 32-33 0.5927156176125337 1106 34-35 0.7441777178929669 1106 36-37 0.8379321198540524 1106 38-39 0.8445043721184078 1106 40-41 0.8165756249620912 1106 42-43 0.793600558736749 1106 44-45 0.7079749020365966 1106 46-47 0.3907614567750315 1106 48-49 0.46512302843951403 1106 50-51 0.23293244866410845 1106 52-53 0.29173524737966616 1106 54-55 0.3678702064438575 1106 56-57 0.01341865870801584 1106 58-59 0.1769685246422945 1106 60-61 0.25558238424455126 1106 62-63 0.2712030477102978 1106 64-65 0.5128449954609842 1106 66-67 1.1187330456536166 1106 68-69 -5.4524419074471275 1106 70-71 -5.6029133823845605 1106 72-73 -2.370028615915313 1106 74-75 0.036525519895992176 1106 76-77 0.102433916361651 1106 78-79 -0.013154481590827771 1106 80-81 -0.19665883430733544 1106 82-83 0.03601023018010707 1106 84-85 0.09103671132519509 1106 86-87 0.1326813016481765 1106 88-89 0.25009439584874116 1106 90-91 0.3524955838963031 1106 92-93 0.17860192417600373 1106 94-95 1.127171871143176 1106 96-97 1.2221103065439927 1106 98-99 0.6559116241456486 1106 100-101 0.6867518857484605 1107 1 -0.06218685708272531 1107 2 -0.07003150191689045 1107 3 -0.10588349911090233 1107 4 0.03553097686214812 1107 5 0.05510936321061877 1107 6 -0.07104773850775103 1107 7 0.07205327197281264 1107 8 0.09678560824079341 1107 9 0.20723177828159578 1107 10-11 -0.045605587190330255 1107 12-13 0.010968746503749571 1107 14-15 0.045599761012134366 1107 16-17 -0.0695440215267169 1107 18-19 0.2556007777799252 1107 20-21 0.08817124427640977 1107 22-23 0.25961816175874475 1107 24-25 0.39969482682700885 1107 26-27 0.22612294848989478 1107 28-29 0.13344913135625802 1107 30-31 0.4724230927394828 1107 32-33 0.4027719440922226 1107 34-35 0.4465653545871717 1107 36-37 0.27320568651447985 1107 38-39 0.17649415848775618 1107 40-41 0.2392024709466014 1107 42-43 0.2950911605923139 1107 44-45 0.2407414056060233 1107 46-47 0.25140912216722455 1107 48-49 0.09588156430753969 1107 50-51 -0.05113081167652922 1107 52-53 0.30033127422386485 1107 54-55 0.16809168002299302 1107 56-57 0.21545871111302972 1107 58-59 0.011562965076148402 1107 60-61 0.11764043973326466 1107 62-63 0.4721535570549662 1107 64-65 0.6442563659234501 1107 66-67 1.1165294503843235 1107 68-69 -5.520285458867317 1107 70-71 -5.67777546272109 1107 72-73 -1.9926256053006206 1107 74-75 -0.12338999038448151 1107 76-77 0.1851188713093812 1107 78-79 0.1646371104343416 1107 80-81 0.3105914174684621 1107 82-83 0.4573358631845714 1107 84-85 0.32286429169291964 1107 86-87 0.4472673644094236 1107 88-89 0.4755651943122423 1107 90-91 0.3751598620350336 1107 92-93 -0.027889880855944682 1107 94-95 1.0807559999097833 1107 96-97 1.106627654564992 1107 98-99 1.2210413443848616 1107 100-101 1.2127293551565863 1108 1 0.05216837406082675 1108 2 0.05806618214186443 1108 3 0.19782020459280147 1108 4 0.2111460079516334 1108 5 0.3186130128456597 1108 6 0.21661559392443763 1108 7 0.1360165052791089 1108 8 0.06856176395855584 1108 9 0.220325407190316 1108 10-11 0.19404927448399434 1108 12-13 0.3787573380149638 1108 14-15 0.2732648957332344 1108 16-17 0.2053681168890762 1108 18-19 0.264657279537154 1108 20-21 0.4536889517649243 1108 22-23 0.3742392305160678 1108 24-25 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2316 94-95 1.0299396640978813 2316 96-97 0.9572001128954817 2316 98-99 0.09140824071703335 2316 100-101 -0.02563956496074482 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 7.0 4 40.0 5 132.0 6 329.0 7 643.0 8 1155.0 9 1781.0 10 2779.0 11 4156.0 12 5403.0 13 6673.0 14 7644.0 15 9696.0 16 11479.0 17 13151.0 18 11511.0 19 9106.0 20 8532.0 21 8518.0 22 9216.0 23 10172.0 24 11990.0 25 14584.0 26 17895.0 27 21991.0 28 26415.0 29 31350.0 30 36688.0 31 42814.0 32 48868.0 33 56834.0 34 67652.0 35 76026.0 36 72016.0 37 57494.0 38 25016.0 39 1616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.51631242725909 19.682955616248012 14.65444733318654 18.146284623306357 2 14.340378684791752 25.106295685947362 39.065801440068924 21.487524189191962 3 21.994812524867477 30.480723347876676 30.627569639187453 16.896894488068398 4 11.362402700659167 18.441925299811178 39.04785083479519 31.147821164734466 5 10.892618844479447 42.92607359278948 32.790110695456235 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17.439924336110277 52-53 18.648643840733904 30.355156224128564 32.77723573675691 18.218964198380625 54-55 16.91485482583273 30.094333620436647 33.71546123181688 19.275350321913738 56-57 18.87675475011866 32.318862187400406 29.838172474684953 18.96621058779598 58-59 18.299455455970158 30.408243920717016 30.709575605740273 20.582725017572553 60-61 18.416610317590003 28.818091506342437 32.04574838524855 20.71954979081901 62-63 22.44601473698891 28.084437559222103 30.788725615533863 18.68082208825512 64-65 21.13417321348849 28.915632996939156 32.26649364962271 17.68370013994964 66-67 18.046386376447987 29.602813476066984 33.543643690448974 18.807156457036058 68-69 18.355927517517014 31.824809868957637 31.458302164780928 18.36096044874442 70-71 18.120112978989305 31.416053478009744 29.953716706495463 20.51011683650549 72-73 18.20717978252225 30.222277071592142 28.774390829185347 22.79615231670026 74-75 18.43005679814576 30.776583689106328 30.45291300194805 20.340446510799865 76-77 20.290415945595385 28.88980207490754 31.050793731107134 19.76898824838994 78-79 18.223754020171963 28.76692847922638 33.191168748769336 19.818148751832325 80-81 17.64534567731588 32.25535417508411 31.183527865911643 18.91577228168837 82-83 17.378451879126633 31.842606480652247 29.765633865981567 21.01330777423955 84-85 18.165802315358565 29.30786721485636 29.951105927585907 22.575224542199166 86-87 19.5503407276148 29.60836833584914 30.842685568025203 19.998605368510862 88-89 20.898134532176876 27.509138652517905 31.427393602293773 20.165333213011444 90-91 18.155198758464625 28.817225619656732 33.071945470891265 19.955630150987382 92-93 17.152928371996357 31.355676122084546 31.45925000205097 20.032145503868126 94-95 16.845562303303304 31.96577811550777 29.9087923465329 21.27986723465603 96-97 16.57471549501161 30.224513444561012 28.776410978222955 24.424360082204416 98-99 18.17279902197747 29.631197168220012 29.279002395143255 22.91700141465926 100-101 19.825822754346913 29.753501867845166 29.953276523127915 20.467398854680006 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8549.0 1 5503.0 2 3398.0 3 4201.0 4 3490.5 5 2977.0 6 3818.0 7 5833.0 8 7420.5 9 7669.0 10 6501.0 11 4881.5 12 4030.0 13 3560.5 14 3311.0 15 3038.0 16 2747.0 17 2640.5 18 2712.0 19 2818.0 20 2985.0 21 3277.5 22 3600.0 23 3999.5 24 4425.0 25 5146.0 26 6097.5 27 7022.5 28 7752.0 29 8675.5 30 9821.0 31 10919.0 32 12251.5 33 13676.5 34 15029.5 35 15965.0 36 16944.0 37 18078.5 38 18342.5 39 18286.0 40 18331.0 41 18029.0 42 17861.0 43 18251.5 44 20197.5 45 22040.0 46 25225.5 47 35815.0 48 61681.0 49 59663.5 50 32162.5 51 21084.5 52 15309.0 53 12058.0 54 10164.0 55 9130.0 56 8087.0 57 6969.5 58 6190.5 59 5433.5 60 4648.0 61 4045.5 62 3227.5 63 2210.0 64 1428.5 65 1015.0 66 784.0 67 581.5 68 455.5 69 389.0 70 323.5 71 273.0 72 260.0 73 268.0 74 213.5 75 138.0 76 105.5 77 70.0 78 46.5 79 31.0 80 23.0 81 16.5 82 8.5 83 6.5 84 3.5 85 2.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025431480618067017 2 0.022149999247993855 3 0.0 4 0.0 5 1.3672839041971514E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.469135616788605E-4 18-19 0.001435648099407009 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.050925856295727E-4 28-29 0.014698301970119378 30-31 0.0056742282024181785 32-33 0.010664814452737781 34-35 0.011416820600046214 36-37 0.10828888521241439 38-39 0.11047653945912984 40-41 0.1245595636723605 42-43 0.11013471848308055 44-45 0.10432376189024264 46-47 0.10575940998964967 48-49 0.10890416296930311 50-51 0.10630632355132852 52-53 0.08203703425182908 54-55 0.07021002848052373 56-57 0.039651233221717395 58-59 0.01681759202162496 60-61 0.009912808305429349 62-63 0.0027345678083943027 64-65 0.005810956592837893 66-67 0.43308717665444774 68-69 0.8413581504477172 70-71 0.9321458016864079 72-73 0.2880867186143398 74-75 0.1585365686916597 76-77 0.0157921290934771 78-79 0.008887345377281485 80-81 0.0011621913185675787 82-83 0.0 84-85 0.0 86-87 1.3672839041971514E-4 88-89 7.520061473084333E-4 90-91 0.0033498455652830206 92-93 0.0020509258562957274 94-95 0.22813131941529474 96-97 0.2368819364021565 98-99 0.014424845189279948 100-101 0.007246604692244902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 731377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.65680367920632 #Duplication Level Percentage of deduplicated Percentage of total 1 83.34599405811358 44.720796406244965 2 10.551129469430872 11.322797650702816 3 2.971904376085297 4.7838866898294885 4 1.1801619302930995 2.5329486801364003 5 0.5472919827908248 1.4682969237905426 6 0.3201937121899995 1.0308342692577057 7 0.20533132841475868 0.7712195944558942 8 0.13801464988456463 0.5924339978968385 9 0.0991907422284721 0.47900323642931 >10 0.5476889191232018 5.350231809223508 >50 0.045651786917964385 1.6929269764306076 >100 0.03359763693564155 3.7214921179050586 >500 0.005129410219172766 2.0168690041773223 >1k 0.006411762773965957 7.840000275449388 >5k 0.00153882306575183 6.295972558004685 >10k+ 7.69411532875915E-4 5.3802898100655545 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 14684 2.007719684923097 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14317 1.9575403656390618 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10096 1.380409829677444 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9830 1.3440400778257997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9010 1.2319227976816334 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8853 1.210456440385738 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6959 0.9514928689307975 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5813 0.7948021335098041 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5286 0.7227462717586142 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4677 0.6394786819930077 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4487 0.6135002878132618 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4347 0.5943583131545017 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3342 0.456946280782688 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3076 0.4205765289310438 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2995 0.40950152930704686 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2761 0.3775070859488335 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2564 0.3505715930361496 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2469 0.33758239594627665 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.3176200509449983 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2224 0.3040839402934465 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2199 0.3006657305329536 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2188 0.2991617182383367 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2130 0.29123147159399326 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1957 0.26757746005138255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1945 0.26593671936634594 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1677 0.2292935107338623 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1494 0.20427221528705442 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1355 0.18526696901871403 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1309 0.17897746305940712 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1285 0.17569598168933395 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1095 0.14971758750958808 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1031 0.14096697052272633 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1026 0.14028332857062772 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1015 0.13877931627601087 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 999 0.1365916620292954 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 991 0.1354978349059377 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 940 0.12852468699453223 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 874 0.11950061322683105 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 872 0.1192271564459916 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 834 0.11403147761004243 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 759 0.10377684832856379 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 739 0.10104228052016948 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 735 0.10049536695849062 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.3672839041971514E-4 0.0 12-13 0.0 0.0 0.0 1.3672839041971514E-4 0.0 14-15 0.0 0.0 0.0 1.3672839041971514E-4 0.0 16-17 0.0 0.0 0.0 4.7854936646900297E-4 0.0 18-19 0.0 0.0 0.0 6.836419520985757E-4 0.0 20-21 0.0 0.0 0.0 0.001093827123357721 0.0 22-23 0.0 0.0 0.0 0.0025978394179745875 0.0 24-25 0.0 0.0 0.0 0.004717129469480172 0.0 26-27 0.0 0.0 0.0 0.007041512106615329 0.0 28-29 0.0 0.0 0.0 0.01264737611382365 0.0 30-31 0.0 0.0 0.0 0.023107097980931857 0.0 32-33 0.0 0.0 0.0 0.04170215907801311 0.0 34-35 0.0 0.0 0.0 0.07424351599790532 0.0 36-37 0.0 0.0 0.0 0.12544829821008863 0.0 38-39 0.0 0.0 0.0 0.18389968511451688 0.0 40-41 0.0 0.0 0.0 0.2492558557351407 0.0 42-43 0.0 0.0 0.0 0.3124243721090491 0.0 44-45 0.0 0.0 0.0 0.38694134488779386 0.0 46-47 0.0 0.0 0.0 0.47998501456841003 0.0 48-49 0.0 0.0 0.0 0.582668035773616 0.0 50-51 0.0 0.0 0.0 0.6882223531776361 0.0 52-53 0.0 0.0 0.0 0.7869402510606704 0.0 54-55 0.0 0.0 0.0 0.8948873152970356 0.0 56-57 0.0 0.0 0.0 1.0102860768112751 0.0 58-59 0.0 0.0 0.0 1.1498173992345944 0.0 60-61 0.0 0.0 0.0 1.3159423935945482 0.0 62-63 0.0 0.0 0.0 1.4568409999220648 0.0 64-65 0.0 0.0 0.0 1.5798965512998084 0.0 66-67 0.0 0.0 0.0 1.704866300143428 0.0 68-69 0.0 0.0 0.0 1.8641548749823962 0.0 70-71 0.0 0.0 0.0 2.0813479231641137 0.0 72-73 0.0 0.0 0.0 2.3334067109028584 0.0 74-75 0.0 0.0 0.0 2.579996363024815 0.0 76-77 0.0 0.0 0.0 2.83191842237314 0.0 78-79 0.0 0.0 0.0 3.121372424891677 0.0 80-81 0.0 0.0 0.0 3.4315407785588006 0.0 82-83 0.0 0.0 0.0 3.762081662398462 0.0 84-85 0.0 6.836419520985757E-5 0.0 4.192434271244515 0.0 86-87 0.0 1.3672839041971514E-4 0.0 4.642407404115798 0.0 88-89 0.0 1.3672839041971514E-4 0.0 5.008292576878956 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGAGG 25 0.005075143 56.84445 6 TATCACG 40 5.450752E-4 47.386578 2 TATAACG 40 5.450752E-4 47.386578 2 GTACGCG 30 0.0036641152 32.158474 64-65 GCAACGC 180 0.0 32.107063 68-69 GCCGTAA 30 0.0036998538 32.09479 62-63 AGTACTC 460 0.0 31.923515 5 GACACGC 30 0.00400451 31.580248 16-17 AAGTACT 470 0.0 30.236414 4 AGCAACG 215 0.0 29.16699 66-67 CATTCCG 65 0.0059212376 29.151001 9 ATCACGC 65 0.0059212376 29.151001 3 GGTATCG 85 3.1559466E-9 28.318937 62-63 CGTCGAT 35 0.0077844122 27.564407 64-65 ATCGACG 80 5.3772965E-8 27.133713 64-65 ATAACGC 70 0.008502178 27.068789 3 GCGTTTA 490 0.0 27.018578 62-63 GTATCGA 90 5.808033E-9 26.745663 62-63 TGTACGC 120 7.2759576E-12 26.077019 62-63 TAGCAAC 255 0.0 25.537611 66-67 >>END_MODULE