FastQCFastQC Report
Fri 27 May 2016
SRR939407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026638
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113030.5577217046162166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91700.4524735053818196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60970.30084307113554565No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23930.11807732806746937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23770.11728784321620338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA172150.030.6217731
GGTATCA116900.030.2629871
GTACATG125450.026.043451
TATCAAC210300.025.2138082
TACATGG125450.025.1814962
ATCAACG212300.024.6607993
TCAACGC214650.024.619574
CAACGCA214500.024.27925
AACGCAG219850.023.7870186
GAGTACT112550.023.51887712-13
ACATGGG129950.023.3326683
AGTACTT117800.022.22668312-13
ACGCAGA241750.021.3216637
GTACTTT124800.021.12223814-15
CGCAGAG242150.021.0195438
CATGGGG46950.021.002644
CATGGGA76200.020.855734
GCAGAGT235000.020.0766629
TACCTGG12300.019.847822
AGAGTAC197300.019.03625710-11
Produced by FastQC (version 0.11.5)