FastQCFastQC Report
Fri 27 May 2016
SRR939407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026638
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162950.8040409782112049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150160.74093153291313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100580.4962899146270819No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37570.18538091163789486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36310.17916371843417522No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28660.14141647398302015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27890.13761707813630258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130100.033.2949031
CATTCCG4100.031.201949
GTATCAA187300.028.5670241
GTACATG133400.025.6856961
TACATGG132800.025.476792
GTGTACG2351.8189894E-1024.2003821
ACATGGG134400.023.6903633
ATCAACG232800.022.8762593
GAGTACT134400.022.86190612-13
TATCAAC233650.022.8561292
TCAACGC234350.022.7249554
CAACGCA236100.022.5765845
AACGCAG240100.022.2004646
AGTACTT136850.021.70823112-13
GTACTTT141500.021.51386514-15
CATGGGG48400.019.9703884
CATGGGA77700.019.8791714
ACGCAGA268200.019.7861357
CGCAGAG268350.019.7044498
AGAGTAC227650.019.04386510-11
Produced by FastQC (version 0.11.5)