##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939407_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2026638 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98707514612871 31.0 31.0 31.0 30.0 34.0 2 31.236462555226932 31.0 31.0 34.0 30.0 34.0 3 31.207499810030207 31.0 31.0 34.0 28.0 34.0 4 35.1540763570011 35.0 35.0 37.0 33.0 37.0 5 34.78767495724446 35.0 35.0 37.0 32.0 37.0 6 34.757688842309285 35.0 35.0 37.0 32.0 37.0 7 34.630779645896304 35.0 35.0 37.0 30.0 37.0 8 34.65632441511508 35.0 35.0 37.0 30.0 37.0 9 35.974172003090835 37.0 35.0 39.0 30.0 39.0 10-11 35.84673656568168 37.0 35.0 39.0 30.0 39.0 12-13 35.803258648066404 37.0 35.0 39.0 30.0 39.0 14-15 36.6156476884377 38.0 35.5 40.0 30.0 41.0 16-17 36.638082874198545 38.0 35.5 40.0 30.5 41.0 18-19 36.67179387734761 38.5 35.0 40.0 30.0 41.0 20-21 36.600831031491566 38.0 35.0 40.0 30.0 41.0 22-23 36.467479391978245 38.0 35.0 40.0 30.0 41.0 24-25 36.24023135853567 38.0 34.5 40.0 29.5 41.0 26-27 35.859734940329744 38.0 34.0 40.0 28.0 41.0 28-29 35.588797308646136 38.0 34.0 40.0 27.0 41.0 30-31 35.32455327493119 38.0 34.0 40.0 27.0 41.0 32-33 35.07066136132846 37.0 33.0 40.0 27.0 41.0 34-35 34.96370368067706 37.5 33.0 40.0 26.5 41.0 36-37 34.89415302584872 38.0 33.0 40.0 25.5 41.0 38-39 34.8449103885351 38.0 33.0 40.0 25.0 41.0 40-41 34.60632806648252 38.0 33.0 40.0 25.0 41.0 42-43 34.25078751113914 37.0 33.0 40.0 24.0 41.0 44-45 33.9860431413997 37.0 33.0 40.0 24.0 41.0 46-47 33.545829348901975 37.0 32.5 40.0 22.0 41.0 48-49 33.13986069539799 37.0 32.0 40.0 19.5 41.0 50-51 32.846154567317896 37.0 31.0 40.0 18.0 41.0 52-53 32.47620344629875 37.0 31.0 40.0 15.0 41.0 54-55 32.266721289149814 36.0 31.0 39.0 14.0 41.0 56-57 31.891061452513966 36.0 30.0 39.0 11.5 40.0 58-59 31.47811029892857 35.5 29.5 39.0 8.5 40.0 60-61 31.215201974896356 35.0 29.0 39.0 8.0 40.0 62-63 30.84609535595405 35.0 29.0 38.5 7.5 40.0 64-65 30.43375087213405 34.5 28.0 38.0 4.5 40.0 66-67 28.7955263840903 33.5 25.0 37.5 2.0 39.5 68-69 25.240193858005227 30.0 15.0 35.0 2.0 39.0 70-71 24.649073490184236 29.5 12.0 35.0 2.0 38.0 72-73 26.427458924583473 31.0 21.0 35.0 2.0 38.0 74-75 27.00940325800661 32.0 22.5 35.0 2.0 38.0 76-77 27.033951796028695 31.5 25.0 34.5 2.0 36.5 78-79 27.84640276161801 33.0 26.0 35.0 2.0 37.0 80-81 27.686256499680752 33.0 26.0 35.0 2.0 37.0 82-83 27.283593567277432 33.0 26.0 35.0 2.0 36.0 84-85 26.947948523613984 32.0 25.5 35.0 2.0 36.0 86-87 26.504491922089688 32.0 24.5 35.0 2.0 35.5 88-89 26.00981255655919 32.0 23.5 34.0 2.0 35.0 90-91 25.558285446142825 32.0 22.0 34.0 2.0 35.0 92-93 25.006932910564196 31.0 19.5 34.0 2.0 35.0 94-95 23.66592208376632 31.0 7.0 34.0 2.0 35.0 96-97 22.87498754094219 30.0 2.0 34.0 2.0 35.0 98-99 22.551159111790067 30.0 2.0 34.0 2.0 35.0 100-101 21.320522461337447 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4001428615322773 1101 2 0.22238366267558973 1101 3 0.34664961128944327 1101 4 0.32761698715771814 1101 5 0.4387103706264881 1101 6 0.47792455414148094 1101 7 0.4010631721633757 1101 8 0.5308782296337142 1101 9 0.6471380365717039 1101 10-11 0.8157695614901854 1101 12-13 0.7337028686640394 1101 14-15 0.7922086463936253 1101 16-17 0.8148048525340457 1101 18-19 0.8774130018905879 1101 20-21 0.909322120093627 1101 22-23 0.855555243445707 1101 24-25 0.7177908218564184 1101 26-27 0.7664961146777358 1101 28-29 0.8456267835022473 1101 30-31 0.9100577479695389 1101 32-33 0.8195032822747308 1101 34-35 0.87119175813514 1101 36-37 0.8691772556485091 1101 38-39 0.8197594707287124 1101 40-41 0.8560721947462682 1101 42-43 1.1538253905457907 1101 44-45 0.8049962336558494 1101 46-47 0.9117054221064578 1101 48-49 0.8034476352294817 1101 50-51 0.8074825423428891 1101 52-53 0.8297139241929514 1101 54-55 0.7535593271142957 1101 56-57 0.7676481352514166 1101 58-59 0.7463059289855138 1101 60-61 0.6377851860047059 1101 62-63 0.6089287984718723 1101 64-65 0.3629229407513819 1101 66-67 -2.624430009504202 1101 68-69 -7.187272411032268 1101 70-71 -10.831412839405413 1101 72-73 -10.80886779066977 1101 74-75 -9.120496782599798 1101 76-77 -3.9375937261569938 1101 78-79 -1.0550059952013306 1101 80-81 -0.40118133580245896 1101 82-83 -0.04760478324421413 1101 84-85 -0.16465955908220664 1101 86-87 -0.09918729117699598 1101 88-89 -0.14874077904203276 1101 90-91 -0.059162350004942965 1101 92-93 -0.6904751387305872 1101 94-95 -4.5142987997211925 1101 96-97 -1.4973805051485165 1101 98-99 -0.8473819574764327 1101 100-101 -0.7033697892085655 1102 1 0.4641552000628124 1102 2 0.36767912415979254 1102 3 0.4873585504074285 1102 4 0.2935449562482191 1102 5 0.4424348802962328 1102 6 0.6141834106165831 1102 7 0.49544008689907315 1102 8 0.5581566993190421 1102 9 0.8229623072613776 1102 10-11 0.7868859247819984 1102 12-13 0.805936542745755 1102 14-15 0.796866441670872 1102 16-17 0.8482313214192558 1102 18-19 0.9502387569939117 1102 20-21 1.0458688054295138 1102 22-23 1.0579548349687613 1102 24-25 0.9638667059247652 1102 26-27 0.8421220424362659 1102 28-29 0.9156056227118299 1102 30-31 0.5719953815404537 1102 32-33 0.7208766281010028 1102 34-35 0.8467228308082468 1102 36-37 1.1222876812856981 1102 38-39 1.0348065824387334 1102 40-41 1.0281167903225068 1102 42-43 1.1863854250768355 1102 44-45 0.9208741959033091 1102 46-47 1.0133561743146586 1102 48-49 1.0840213979549063 1102 50-51 1.0205760182922319 1102 52-53 1.0838047997864564 1102 54-55 1.1323425290261397 1102 56-57 0.9993994275335218 1102 58-59 1.0345264223161763 1102 60-61 1.0066716652071257 1102 62-63 0.8677118951061473 1102 64-65 0.7911365910403703 1102 66-67 -1.9924061536081297 1102 68-69 -7.1316290071980575 1102 70-71 -10.435102502201373 1102 72-73 -8.628469315088903 1102 74-75 -6.851885077840201 1102 76-77 -3.2059356045192935 1102 78-79 -0.2780583076230343 1102 80-81 0.24839871199355557 1102 82-83 0.434714902607535 1102 84-85 0.28803133546125537 1102 86-87 0.24290531096855972 1102 88-89 0.3979176057895728 1102 90-91 0.15688100329603927 1102 92-93 -0.033842546902231874 1102 94-95 -3.6032693956974846 1102 96-97 -0.49927655408152916 1102 98-99 0.2538304189540277 1102 100-101 0.29481227781112906 1103 1 0.3304927463007665 1103 2 0.3381955210455878 1103 3 0.44125049123916327 1103 4 0.3012499224185561 1103 5 0.45629260402267846 1103 6 0.43510661992819877 1103 7 0.369703440338526 1103 8 0.46215834696034364 1103 9 0.7502660483043613 1103 10-11 0.7858659369353944 1103 12-13 0.6935478299043538 1103 14-15 0.7742000564082829 1103 16-17 0.8238232895757349 1103 18-19 0.7758940410692929 1103 20-21 0.8476502156310275 1103 22-23 0.6695233976464223 1103 24-25 0.6858486471235423 1103 26-27 0.780731396471154 1103 28-29 0.8437244160184818 1103 30-31 0.9881071927631737 1103 32-33 0.9486097142881462 1103 34-35 1.028462866452756 1103 36-37 1.0681768366248292 1103 38-39 1.11143556542806 1103 40-41 1.1965917841030702 1103 42-43 1.0562284913209936 1103 44-45 0.9164041532033025 1103 46-47 1.1526754619137094 1103 48-49 1.1114803190761293 1103 50-51 1.013404394427532 1103 52-53 1.0676973727577987 1103 54-55 1.065914240166883 1103 56-57 1.2411532682914306 1103 58-59 0.9988829245857573 1103 60-61 0.8595283244920289 1103 62-63 0.9334144469110086 1103 64-65 0.8780067454863527 1103 66-67 -2.4587951886368202 1103 68-69 -7.185297761964595 1103 70-71 -11.210845062326007 1103 72-73 -8.779512039569834 1103 74-75 -6.967714465398878 1103 76-77 -3.2385229111559433 1103 78-79 -0.3486850230664089 1103 80-81 0.24242122024196178 1103 82-83 0.6182762140321323 1103 84-85 0.32599621296891357 1103 86-87 0.5151223673187104 1103 88-89 0.6050482425376842 1103 90-91 0.6394999169131381 1103 92-93 0.6984972057068752 1103 94-95 1.4051543743831445 1103 96-97 1.5098214642627568 1103 98-99 1.22448374543578 1103 100-101 0.8303940909506622 1104 1 0.43221125116110315 1104 2 0.18044971419744726 1104 3 0.31423802095918774 1104 4 0.16676984090077696 1104 5 0.4079367077314089 1104 6 0.45523698368513266 1104 7 0.6045239879956981 1104 8 0.4402682342264299 1104 9 0.7424037874798017 1104 10-11 0.7726039316626157 1104 12-13 0.7065789489947178 1104 14-15 0.821284259102903 1104 16-17 0.8600833377747463 1104 18-19 0.8851840761672349 1104 20-21 0.8695035321432272 1104 22-23 0.6704675644343894 1104 24-25 0.8603779789688488 1104 26-27 1.067784787384511 1104 28-29 1.0296520851598032 1104 30-31 0.8906299578981489 1104 32-33 0.89044499660816 1104 34-35 1.0175566280779407 1104 36-37 1.0125522713054735 1104 38-39 1.031510128321294 1104 40-41 1.195155114039828 1104 42-43 1.2784158043072296 1104 44-45 1.0645396765183648 1104 46-47 0.9491725424772142 1104 48-49 1.2060312434295568 1104 50-51 1.0969184740367623 1104 52-53 1.2585880839357628 1104 54-55 1.1615189112652686 1104 56-57 1.1243046422866136 1104 58-59 1.1678321700610397 1104 60-61 1.0109965341738878 1104 62-63 1.035589063178982 1104 64-65 0.9388713353904379 1104 66-67 -2.055846234296485 1104 68-69 -6.834919690895319 1104 70-71 -8.351857079729406 1104 72-73 -7.55910348020257 1104 74-75 -6.482135753832733 1104 76-77 -3.032809792292017 1104 78-79 -0.01279940674991309 1104 80-81 0.532341481616438 1104 82-83 0.6200468805694079 1104 84-85 0.8751195733130999 1104 86-87 1.0297551329389094 1104 88-89 0.8973165880825107 1104 90-91 0.8924108619695055 1104 92-93 1.0415039018105183 1104 94-95 2.102125357934021 1104 96-97 2.1872443539387874 1104 98-99 1.7208680112096353 1104 100-101 1.5801615873519133 1105 1 0.5832397223119443 1105 2 0.3846853381846387 1105 3 0.4679619186268731 1105 4 0.2245616471279348 1105 5 0.4235758475908611 1105 6 0.5416383545483043 1105 7 0.5996200115535615 1105 8 0.5231109135937047 1105 9 0.7062530993770793 1105 10-11 0.7349808769430268 1105 12-13 0.7865896092851727 1105 14-15 0.975800191259097 1105 16-17 0.9520032504202618 1105 18-19 0.9042756688648126 1105 20-21 0.9753340310966294 1105 22-23 0.9427871934530501 1105 24-25 0.9190146354743831 1105 26-27 1.0479141111967039 1105 28-29 0.9635117416669488 1105 30-31 1.0617861016088952 1105 32-33 1.1336402588092156 1105 34-35 1.2617021697777275 1105 36-37 1.1372628974376724 1105 38-39 1.0400958307429704 1105 40-41 1.1406518495788234 1105 42-43 1.2820475475277817 1105 44-45 1.1763741773302314 1105 46-47 1.409991118417821 1105 48-49 1.2579343926266802 1105 50-51 1.4117847630739035 1105 52-53 1.5735241869479992 1105 54-55 1.5187989102291866 1105 56-57 1.5360516833882265 1105 58-59 1.7228550933279934 1105 60-61 1.7319391483005155 1105 62-63 1.8059747439077327 1105 64-65 1.72583065144822 1105 66-67 2.4601328692677917 1105 68-69 -4.856413896133027 1105 70-71 -4.721758635953737 1105 72-73 -0.7290240177337495 1105 74-75 1.788217751759671 1105 76-77 1.7948597727001392 1105 78-79 1.8149361273897284 1105 80-81 1.8297811906722607 1105 82-83 2.0452999916420254 1105 84-85 1.8703552248211608 1105 86-87 1.8092384852400585 1105 88-89 1.7322265705500968 1105 90-91 1.5983608743538973 1105 92-93 1.8212746043859873 1105 94-95 2.6565800561514763 1105 96-97 2.4206873120763035 1105 98-99 2.1530292657080814 1105 100-101 1.9698235339193282 1106 1 0.20204217383213674 1106 2 0.27579644024921635 1106 3 0.1460427552865049 1106 4 0.12168491723278407 1106 5 0.3344350805850169 1106 6 0.4331736485821338 1106 7 0.3520453759536011 1106 8 0.23393350812602876 1106 9 0.4534029323667568 1106 10-11 0.5404222991667069 1106 12-13 0.44328447399765736 1106 14-15 0.5390410260460996 1106 16-17 0.4432399409901322 1106 18-19 0.4302399219388775 1106 20-21 0.6105200832413047 1106 22-23 0.5100415681871624 1106 24-25 0.6940452184126613 1106 26-27 0.6506086665177264 1106 28-29 0.9980001364341646 1106 30-31 0.6867751609571044 1106 32-33 0.8207290684052353 1106 34-35 0.9422605806455095 1106 36-37 0.8186654177567547 1106 38-39 0.8249439948507131 1106 40-41 0.9045209322127974 1106 42-43 0.9884707787551505 1106 44-45 0.9143878027670951 1106 46-47 0.9445785388054944 1106 48-49 0.7339804715988478 1106 50-51 0.7181534437025405 1106 52-53 0.9719003906741364 1106 54-55 0.9578986625124841 1106 56-57 0.8918131832854392 1106 58-59 0.933386615635726 1106 60-61 0.8818840888342514 1106 62-63 1.0358283732526985 1106 64-65 0.8636426635714791 1106 66-67 1.5361059766368044 1106 68-69 -5.560394359690989 1106 70-71 -6.27507339901484 1106 72-73 -2.2326921384045626 1106 74-75 0.6435244258625872 1106 76-77 0.7426766292422435 1106 78-79 0.7527954641928609 1106 80-81 0.7222907624704185 1106 82-83 0.8571461223151324 1106 84-85 0.8781274638586289 1106 86-87 0.7345913848689918 1106 88-89 0.6970138537470874 1106 90-91 0.66551978554023 1106 92-93 0.6722221157184656 1106 94-95 1.653192932128249 1106 96-97 1.733749737908859 1106 98-99 1.4997816731603457 1106 100-101 1.5090109131905116 1107 1 0.018507170628808467 1107 2 0.014367497698994924 1107 3 -0.13724747027118056 1107 4 -0.13311256937171834 1107 5 -0.09599186751901811 1107 6 0.030981931278063257 1107 7 -0.005298108665776624 1107 8 -0.0381811947822186 1107 9 -0.007232371711246799 1107 10-11 -0.011167410029173652 1107 12-13 0.1508609890056789 1107 14-15 0.05212643533191397 1107 16-17 -0.07454375009115921 1107 18-19 0.08292466178629354 1107 20-21 0.12238781430259849 1107 22-23 0.21786472384193445 1107 24-25 0.2990591753959677 1107 26-27 0.16595358564794793 1107 28-29 -0.02059396012932524 1107 30-31 0.001030845439231598 1107 32-33 0.06163405224370422 1107 34-35 0.13945924797871356 1107 36-37 0.19692108981887912 1107 38-39 -0.026810589943266905 1107 40-41 0.009655466767846121 1107 42-43 0.22158841796014883 1107 44-45 0.08030305898666512 1107 46-47 -0.1788653636436237 1107 48-49 0.1412992714535406 1107 50-51 0.08715465775826914 1107 52-53 0.02552201307899793 1107 54-55 0.04194123001780525 1107 56-57 0.1607549883058077 1107 58-59 -0.027013329302238986 1107 60-61 0.08150931511126203 1107 62-63 0.09531513714732398 1107 64-65 -0.09277190799519275 1107 66-67 0.6756878156880006 1107 68-69 -5.626583373573453 1107 70-71 -5.799920244798713 1107 72-73 -2.313942377748454 1107 74-75 -0.08184820023412342 1107 76-77 -0.08901146711217578 1107 78-79 0.1743678785284466 1107 80-81 -8.588893036254319E-4 1107 82-83 0.11298295653805113 1107 84-85 0.10709278409299827 1107 86-87 0.1907602472894645 1107 88-89 -0.09734063189383235 1107 90-91 -0.09665539245400012 1107 92-93 0.015207554294800474 1107 94-95 0.6798733850031198 1107 96-97 0.6781427773227193 1107 98-99 0.4979923640957047 1107 100-101 0.36178466309089075 1108 1 0.03817983447787299 1108 2 -0.008031649863976753 1108 3 0.07508332588869493 1108 4 -0.05390287102824942 1108 5 0.1320974469781575 1108 6 0.06772871570819206 1108 7 0.12271598100986836 1108 8 -0.05392951949049518 1108 9 0.20354836873732296 1108 10-11 0.21308712363934035 1108 12-13 0.2146155855002334 1108 14-15 0.20804433625982455 1108 16-17 0.04112148613890554 1108 18-19 0.18401727319434258 1108 20-21 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2311 60-61 0.42500684649229115 2311 62-63 0.5355426620827011 2311 64-65 0.37559455068285175 2311 66-67 1.5932253101228078 2311 68-69 3.9479539680262654 2311 70-71 4.407767028025216 2311 72-73 2.3493200533926917 2311 74-75 1.5288190246128757 2311 76-77 0.7796942190177063 2311 78-79 0.37782627083619147 2311 80-81 0.2341270565130138 2311 82-83 0.1952836562569651 2311 84-85 0.1647023103706644 2311 86-87 0.4002509741864486 2311 88-89 0.5609008342598436 2311 90-91 0.6201860378166515 2311 92-93 0.4931929884461361 2311 94-95 1.1216578343648678 2311 96-97 1.3105624226124064 2311 98-99 0.8373085267388234 2311 100-101 0.7400983604375391 2312 1 -0.23118405337225312 2312 2 -0.1484382772139199 2312 3 -0.13635386169600494 2312 4 0.0010361926207096417 2312 5 -0.05887114195392229 2312 6 -0.03561920987135636 2312 7 0.028016763113093646 2312 8 0.045661131408309075 2312 9 -1.5480692435091896 2312 10-11 -1.101128051381842 2312 12-13 -2.0444483893773153 2312 14-15 -2.0711434439919216 2312 16-17 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1.0512697936230673 2312 84-85 1.0842780297573498 2312 86-87 1.305533477862145 2312 88-89 1.3090839890592534 2312 90-91 1.510177636220348 2312 92-93 1.6127161978559243 2312 94-95 2.3962220015566302 2312 96-97 2.5015307065863652 2312 98-99 2.384641019912525 2312 100-101 2.041831189675097 2313 1 -0.3975420681747259 2313 2 -0.3094071663876079 2313 3 -0.1947168320162973 2313 4 -0.21133978593386615 2313 5 -0.2444403323034834 2313 6 -0.21367181952820147 2313 7 -3.7038192348790666 2313 8 -1.1641815522609065 2313 9 -0.8512379981149394 2313 10-11 -0.18429744678731197 2313 12-13 -1.6358307011690911 2313 14-15 -0.5576941259096273 2313 16-17 -0.2890527220290906 2313 18-19 0.0907818101943576 2313 20-21 -0.17997232216099945 2313 22-23 -1.485393383541549 2313 24-25 -2.700599665595959 2313 26-27 -2.3085150533685166 2313 28-29 -0.7422276377301742 2313 30-31 -0.4458140165817639 2313 32-33 -2.638682238681781 2313 34-35 -2.9048284757368847 2313 36-37 -1.4109764692233853 2313 38-39 -0.7945500812355561 2313 40-41 -0.9976592465886469 2313 42-43 -1.0485737685660155 2313 44-45 -0.6561718125930014 2313 46-47 -0.6838339288329749 2313 48-49 -0.8094770789999188 2313 50-51 -0.5268651123673962 2313 52-53 -0.37842035513958905 2313 54-55 -0.379230056672327 2313 56-57 -0.07915517574582154 2313 58-59 -0.44020701280926033 2313 60-61 -0.3237362960607264 2313 62-63 -0.48256509194988695 2313 64-65 -0.36124733910671836 2313 66-67 1.0200450923221034 2313 68-69 4.146901810489219 2313 70-71 3.972534079527712 2313 72-73 2.1823790888467194 2313 74-75 1.376903033455907 2313 76-77 0.5448829523346639 2313 78-79 0.040091889439317185 2313 80-81 0.11077898478616532 2313 82-83 -0.23329654516774667 2313 84-85 -0.020972789932294233 2313 86-87 0.25334194282642386 2313 88-89 0.23123162931429064 2313 90-91 0.28501449929212797 2313 92-93 0.35644116661693914 2313 94-95 0.7833953272003669 2313 96-97 0.5968843746307506 2313 98-99 0.4045074131713129 2313 100-101 0.21246387259671806 2314 1 -0.30329161424415574 2314 2 -0.1569118142025907 2314 3 -0.05112068916018586 2314 4 -0.18730425275533946 2314 5 -1.3274058191123643 2314 6 -2.60167286121748 2314 7 -0.7166088642836357 2314 8 -0.3448654502042956 2314 9 0.011970838882390922 2314 10-11 0.24716699471532877 2314 12-13 -0.0013091910090068382 2314 14-15 -2.602206087251375 2314 16-17 -2.7403653228523126 2314 18-19 -0.5285885465118838 2314 20-21 -0.13174210883172321 2314 22-23 -0.03655624153181236 2314 24-25 -2.090815020385193 2314 26-27 -1.1327737540058251 2314 28-29 -0.26194341570568014 2314 30-31 0.02979779501715285 2314 32-33 -0.18464316086613763 2314 34-35 -0.20626767074256236 2314 36-37 0.31985319287865366 2314 38-39 -1.3032083999201305 2314 40-41 -3.818517627485221 2314 42-43 -5.319486404112215 2314 44-45 -2.219738006882519 2314 46-47 -2.922615514081496 2314 48-49 -0.6268298861354893 2314 50-51 -0.5509878146829834 2314 52-53 -2.1160705500946335 2314 54-55 -1.056744397984712 2314 56-57 -3.644338881989448 2314 58-59 -4.913140814902768 2314 60-61 -2.2157782240539525 2314 62-63 -1.2558012732906292 2314 64-65 -0.4928278855595494 2314 66-67 -0.5212021859029718 2314 68-69 1.6285897811482997 2314 70-71 1.594612828343152 2314 72-73 -0.35779611750179896 2314 74-75 0.038924055324148554 2314 76-77 0.0012842775949053475 2314 78-79 0.22330803272946653 2314 80-81 0.6060526814086309 2314 82-83 0.530340903561612 2314 84-85 0.13505309315255687 2314 86-87 -0.01848723460074808 2314 88-89 0.25378311697678413 2314 90-91 0.7588568859179965 2314 92-93 0.7124977940032373 2314 94-95 0.4367493496400918 2314 96-97 0.8756013606764554 2314 98-99 0.22934555346660446 2314 100-101 0.1192750013104451 2315 1 -0.31740609593769875 2315 2 -0.3580752780553986 2315 3 -0.2746644803361882 2315 4 -0.17171832885158267 2315 5 -1.185003408971447 2315 6 -0.42067652170399583 2315 7 -0.2898145682360891 2315 8 -0.23801163434171002 2315 9 0.10395275387786285 2315 10-11 -1.7305410360753513 2315 12-13 -1.346004930403133 2315 14-15 -0.1866178369567777 2315 16-17 -1.7528056979023106 2315 18-19 -3.0822102896765458 2315 20-21 -1.935566963812569 2315 22-23 -1.5072099394642748 2315 24-25 -1.0907250964313349 2315 26-27 -0.4482046150120311 2315 28-29 -0.5563246896909959 2315 30-31 -3.0495455543235366 2315 32-33 -2.8021509520385663 2315 34-35 -1.717257854267956 2315 36-37 -4.923701362328838 2315 38-39 -2.437368899414139 2315 40-41 -1.9401997243865523 2315 42-43 -1.8416401320193359 2315 44-45 -1.5556216461809882 2315 46-47 -1.6476692223932403 2315 48-49 -4.9655910919575525 2315 50-51 -4.452898228950559 2315 52-53 -2.7520313181973073 2315 54-55 -4.3816474724891705 2315 56-57 -6.765200255043222 2315 58-59 -7.368781910060207 2315 60-61 -6.355815321040293 2315 62-63 -6.651398668090785 2315 64-65 -6.0589146098312305 2315 66-67 -4.234323075013762 2315 68-69 -2.009049343735043 2315 70-71 0.2862633668578951 2315 72-73 -1.501161519238181 2315 74-75 -3.247801155862927 2315 76-77 -5.281407777154019 2315 78-79 -4.311528999117932 2315 80-81 -3.9622877339952964 2315 82-83 -7.500184699711799 2315 84-85 -5.354521096793679 2315 86-87 -5.600078682788002 2315 88-89 -4.0849138894055805 2315 90-91 -3.813297692016544 2315 92-93 -4.867647712796536 2315 94-95 -2.817785546669981 2315 96-97 -1.7988593863992293 2315 98-99 -3.1614290541562617 2315 100-101 -2.396089468080067 2316 1 -0.47747945293356864 2316 2 -0.4475404389622142 2316 3 -0.9464722950273128 2316 4 -0.2888687623603232 2316 5 -0.16859150338570572 2316 6 -2.367287596543683 2316 7 -0.40239689001423073 2316 8 -0.5080244572030423 2316 9 -0.09247291919188427 2316 10-11 -0.07013053750767995 2316 12-13 -0.5202186625029199 2316 14-15 -1.0354129733503683 2316 16-17 -1.5538548553354232 2316 18-19 -0.2804364866204523 2316 20-21 -4.071662300768871 2316 22-23 -4.13730770530119 2316 24-25 -2.766218244263733 2316 26-27 -2.0971546486479014 2316 28-29 -1.1990754413444762 2316 30-31 -0.31067500990252483 2316 32-33 -1.665686116269832 2316 34-35 -2.4332150362445617 2316 36-37 -4.579071176885318 2316 38-39 -2.3232094231004226 2316 40-41 -1.544026803117461 2316 42-43 -3.004625379727056 2316 44-45 -1.023287629692362 2316 46-47 -0.905495890917841 2316 48-49 -0.6457063672367767 2316 50-51 -0.2157418354468561 2316 52-53 -2.791550990616777 2316 54-55 -1.0633192876103337 2316 56-57 0.09089457045991978 2316 58-59 0.12420335330424948 2316 60-61 -1.2202515300451076 2316 62-63 -0.9413320695830834 2316 64-65 0.16780735640346478 2316 66-67 1.2037227250946856 2316 68-69 3.572786292964121 2316 70-71 3.916766641984726 2316 72-73 1.8836780175522634 2316 74-75 1.0371012166012683 2316 76-77 -0.13239876983762855 2316 78-79 0.17305434139140274 2316 80-81 -0.25367695037077453 2316 82-83 -0.22984595593469592 2316 84-85 -0.055974667545655166 2316 86-87 -0.33086226555912646 2316 88-89 0.26704573293323364 2316 90-91 0.4107563675385215 2316 92-93 0.5827982169496337 2316 94-95 1.1287782970361917 2316 96-97 1.0995140881786796 2316 98-99 0.12993641617971363 2316 100-101 -0.06011432261600547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 29.0 4 59.0 5 141.0 6 395.0 7 1026.0 8 2196.0 9 3743.0 10 5999.0 11 9065.0 12 12050.0 13 15348.0 14 18774.0 15 24112.0 16 29059.0 17 32921.0 18 28712.0 19 21976.0 20 20354.0 21 20558.0 22 21698.0 23 24647.0 24 29931.0 25 37234.0 26 46612.0 27 57458.0 28 70703.0 29 83750.0 30 99226.0 31 115330.0 32 133893.0 33 155677.0 34 181672.0 35 209090.0 36 224386.0 37 196177.0 38 86877.0 39 5756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79241418453717 21.246403277151245 15.941366631295809 21.01981590701577 2 15.675135471262324 22.260519380533594 39.22310068796699 22.841244460237085 3 19.05796693834814 26.75490146735628 31.424704362594603 22.762427231700975 4 12.550588708985028 18.550722921409744 37.59383767599344 31.304850693611787 5 12.29678146445637 39.34125319001537 34.13262174361849 14.229343601909767 6 29.450153406775158 38.029337257073045 17.55537989517615 14.965129440975645 7 26.469206636804405 35.182849625833526 20.804504800561325 17.54343893680075 8 26.3339580132219 34.96786303227316 19.567332695824316 19.130846258680634 9 26.3363758105789 16.9745164158572 21.306617165966493 35.38249060759741 10-11 24.47333959000078 27.994639397859906 26.723322073305646 20.808698938833675 12-13 25.802239965894252 25.88580200312044 27.85842858961492 20.45352944137039 14-15 22.73928545699824 26.611980037875536 27.50789731565282 23.140837189473405 16-17 20.40633958838071 30.038388852429897 28.192714012917996 21.362557546271397 18-19 20.13439211450469 27.573673632825702 32.160922171886305 20.1310120807833 20-21 21.425360621877218 26.25271508774631 32.29508673971375 20.026837550662723 22-23 20.928675964824503 26.568360999843087 32.09292927498645 20.410033760345954 24-25 20.716625268054777 26.910158597638056 32.31697027293478 20.056245861372382 26-27 20.522730238916505 26.821886695070162 32.5233756458078 20.13200742020553 28-29 19.976113274406163 27.097614782132336 32.728737606293464 20.19753433716804 30-31 20.343344250969942 26.90359788555603 32.45952764259235 20.293530220881674 32-33 20.346417759091615 26.973014451344834 32.20476151703344 20.475806272530107 34-35 20.81343886254661 27.016343835672885 32.09120116421978 20.07901613756072 36-37 20.828081194445048 26.645483588630093 32.16059986999721 20.365835346927646 38-39 20.46329223780267 27.163479764614706 32.11838120869218 20.25484678889045 40-41 20.507659953493405 27.06178500555026 32.276758212633055 20.15379682832328 42-43 20.48798330070002 27.21566537384738 31.69372333930678 20.602627986145823 44-45 20.618991300327743 27.372146774463214 31.342256669349478 20.666605255859565 46-47 20.890960198874737 27.729724176389208 30.652512038233898 20.726803586502164 48-49 21.012379559476763 27.7950573634497 30.413223303781045 20.77933977329249 50-51 20.81743908239688 28.071144667607438 30.17155665449459 20.93985959550109 52-53 21.183374563674022 28.40705864749503 29.748363646419723 20.661203142411217 54-55 20.758628453714532 28.056133400024784 29.988490292788978 21.196747853471702 56-57 21.028215570801287 28.517468844551384 29.595214254563334 20.859101330083995 58-59 21.110249110074406 28.024056738092668 29.75919573329227 21.106498418540657 60-61 21.03628973160334 28.142171422706113 30.110844579240155 20.710694266450393 62-63 20.969449472723564 27.968386358685155 30.067796033096915 20.994368135494366 64-65 20.716477007214806 28.10369661988219 29.95565812841378 21.224168244489217 66-67 20.457995484363124 28.202042665410037 30.072045541880144 21.267916308346692 68-69 20.591986374857555 27.867773643168704 30.326735810417922 21.213504171555826 70-71 20.25742177951449 28.067497108753198 30.595340450223013 21.0797406615093 72-73 20.992339216068544 27.961737645117967 29.67029731679583 21.375625822017653 74-75 20.456901141882234 28.529241351875896 29.56075275525571 21.45310475098616 76-77 20.617467675825278 28.498671352363175 29.354439365970187 21.52942160584136 78-79 20.548548192889996 28.559444148358697 29.276021002348944 21.61598665640236 80-81 20.508119595807678 28.75842632732157 29.181127395287547 21.552326681583207 82-83 20.693286122139227 28.820662594898543 28.995804874871585 21.49024640809064 84-85 20.458389707485996 28.61218925136112 28.981470790540786 21.9479502506121 86-87 20.517873734415655 28.53328649044992 29.1721046681861 21.776735106948323 88-89 20.627700463752568 28.45664911444896 28.88707031586344 22.02858010593503 90-91 20.359091002172892 28.56439343192891 29.173578003924383 21.90293756197382 92-93 20.5307310121726 28.54414855218171 29.125343370500655 21.799777065145037 94-95 20.094140128144517 28.25412988133082 29.309868195908003 22.341861794616662 96-97 20.076169628640294 28.271613150673282 29.22320183962438 22.429015381062047 98-99 20.195011984679745 28.285250676466944 28.74373808502732 22.775999253825994 100-101 20.04441704053024 28.173090754411174 29.09281611981794 22.68967608524065 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3283.0 1 2168.5 2 1137.0 3 1223.0 4 1135.0 5 1123.5 6 1750.0 7 3314.5 8 5044.0 9 5991.0 10 5662.5 11 4906.0 12 4684.5 13 4788.0 14 5001.0 15 5268.0 16 5592.0 17 5914.5 18 6262.0 19 6667.5 20 7258.5 21 8018.5 22 9096.0 23 10536.5 24 12069.5 25 14467.0 26 18407.5 27 22303.0 28 24778.0 29 27506.0 30 31262.5 31 35545.0 32 40335.5 33 45392.5 34 51216.5 35 56041.0 36 61103.0 37 65864.0 38 66416.5 39 64521.0 40 62954.5 41 63323.0 42 64405.0 43 65135.5 44 66009.0 45 66239.5 46 66478.5 47 69114.0 48 75777.0 49 76087.0 50 68115.5 51 61786.0 52 57652.5 53 55461.5 54 52818.0 55 48191.5 56 42999.5 57 37993.0 58 34008.0 59 29828.5 60 25093.5 61 20643.5 62 16292.5 63 12451.5 64 9479.5 65 7125.5 66 5311.0 67 4046.0 68 2890.0 69 1919.0 70 1347.5 71 1028.0 72 845.0 73 730.0 74 575.5 75 426.5 76 276.5 77 147.0 78 86.0 79 54.0 80 33.5 81 19.0 82 11.5 83 5.0 84 3.5 85 3.5 86 2.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024079287963612642 2 0.018651579611158972 3 0.0 4 0.0 5 9.868560640824855E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.947424256329942E-4 18-19 0.0015296268993278523 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.6875663043918054E-4 28-29 0.020131863707282704 30-31 0.004958951722014489 32-33 0.009029732986354741 34-35 0.010682716893692904 36-37 0.10194223141972074 38-39 0.10497681381677439 40-41 0.11886681291873537 42-43 0.10470542839915169 44-45 0.09974647667713721 46-47 0.10100471815884238 48-49 0.10317580149982386 50-51 0.10159683179729187 52-53 0.08055212623073287 54-55 0.06863583925693686 56-57 0.03890680032645198 58-59 0.017023267105422873 60-61 0.009720532231212482 62-63 0.00219575474258353 64-65 0.005477051155657794 66-67 0.4213628679616192 68-69 0.8011790956253657 70-71 0.8876276868389914 72-73 0.28105660705069185 74-75 0.1482997950299955 76-77 0.016258453655758946 78-79 0.009769875034416606 80-81 0.0014062698913175416 82-83 0.0 84-85 0.0 86-87 2.4671401602062134E-4 88-89 9.12841859276299E-4 90-91 0.0037747244451155067 92-93 0.001825683718552598 94-95 0.22431238336594891 96-97 0.23208387487059848 98-99 0.014852183764441404 100-101 0.008067548323874317 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2026638.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.93546366337926 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34620085159384 34.42537944916899 2 13.924646846684016 13.071195121958523 3 5.162739564801582 7.269467257417052 4 2.4636986811559067 4.6253935970763385 5 1.3817396504675865 3.242629557838588 6 0.8442089693533444 2.377400364322965 7 0.5389532002110092 1.7707212841336053 8 0.3755269150868201 1.4100423902142685 9 0.25437642290197754 1.0745347818542548 >10 1.4655422488719085 12.78183053261718 >50 0.14217400277891168 4.663699894027797 >100 0.09229781784285573 7.997139805510872 >500 0.005761090885372018 1.7202413811218717 >1k 0.0018136767602097096 1.4988142580118724 >5k 0.0 0.0 >10k+ 3.2006060474288993E-4 2.071510324725817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16295 0.8040409782112049 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15016 0.74093153291313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10058 0.4962899146270819 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3757 0.18538091163789486 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3631 0.17916371843417522 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2866 0.14141647398302015 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2789 0.13761707813630258 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4671401602062134E-5 0.0 12-13 4.934280320412427E-5 0.0 0.0 4.934280320412427E-5 0.0 14-15 9.868560640824853E-5 0.0 0.0 4.934280320412427E-5 0.0 16-17 9.868560640824853E-5 0.0 0.0 7.40142048061864E-5 0.0 18-19 1.480284096123728E-4 0.0 0.0 2.7138541762268347E-4 0.0 20-21 1.9737121281649707E-4 0.0 0.0 4.6875663043918054E-4 0.0 22-23 1.9737121281649707E-4 0.0 0.0 8.388276544701125E-4 0.0 24-25 2.960568192247456E-4 0.0 0.0 0.0013322556865113552 0.0 26-27 2.960568192247456E-4 0.0 0.0 0.0019490407265629086 0.0 28-29 3.9474242563299414E-4 0.0 0.0 0.003626696035503134 0.0 30-31 4.440852288371184E-4 0.0 0.0 0.008240248135088753 0.0 32-33 4.440852288371184E-4 0.0 0.0 0.017418009531055868 0.0 34-35 4.440852288371184E-4 0.0 0.0 0.03239355030350758 0.0 36-37 4.934280320412427E-4 0.0 0.0 0.059458077860969745 0.0 38-39 4.934280320412427E-4 0.0 0.0 0.09898166322747329 0.0 40-41 4.934280320412427E-4 0.0 0.0 0.14213194462947998 0.0 42-43 5.427708352453669E-4 0.0 0.0 0.18557828285071137 0.0 44-45 6.414564416536155E-4 0.0 0.0 0.23738822621504185 0.0 46-47 7.894848512659883E-4 0.0 0.0 0.3042230531550282 0.0 48-49 8.388276544701125E-4 0.0 0.0 0.3785579861820414 0.0 50-51 8.388276544701125E-4 0.0 0.0 0.4583699703647124 0.0 52-53 8.388276544701125E-4 0.0 0.0 0.5341358446846451 0.0 54-55 8.388276544701125E-4 0.0 0.0 0.6202883790790461 0.0 56-57 8.388276544701125E-4 0.0 0.0 0.7181598292344267 0.0 58-59 8.388276544701125E-4 0.0 0.0 0.8184984195500133 0.0 60-61 8.388276544701125E-4 0.0 0.0 0.9192810950944372 0.0 62-63 8.388276544701125E-4 4.934280320412427E-5 0.0 1.0182134155187064 0.0 64-65 8.388276544701125E-4 4.934280320412427E-5 0.0 1.1257313837004932 0.0 66-67 8.388276544701125E-4 4.934280320412427E-5 0.0 1.2422297420654305 0.0 68-69 8.388276544701125E-4 7.40142048061864E-5 0.0 1.379772805996927 0.0 70-71 8.388276544701125E-4 9.868560640824853E-5 0.0 1.564092847365933 0.0 72-73 8.388276544701125E-4 9.868560640824853E-5 0.0 1.7576153215325085 0.0 74-75 8.388276544701125E-4 9.868560640824853E-5 0.0 1.966606764503577 0.0 76-77 8.388276544701125E-4 9.868560640824853E-5 0.0 2.1983945825549505 0.0 78-79 8.388276544701125E-4 9.868560640824853E-5 0.0 2.4680283306638877 0.0 80-81 0.0010361988672866096 9.868560640824853E-5 0.0 2.7682792881609837 0.0 82-83 0.0010361988672866096 9.868560640824853E-5 0.0 3.074007296813738 0.0 84-85 0.001110213072092796 9.868560640824853E-5 0.0 3.3786250923943992 0.0 86-87 0.0012088986785010445 9.868560640824853E-5 0.0 3.6917791929293737 0.0 88-89 0.0012335700801031067 9.868560640824853E-5 0.0 4.033157376897107 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13010 0.0 33.294903 1 CATTCCG 410 0.0 31.20194 9 GTATCAA 18730 0.0 28.567024 1 GTACATG 13340 0.0 25.685696 1 TACATGG 13280 0.0 25.47679 2 GTGTACG 235 1.8189894E-10 24.200382 1 ACATGGG 13440 0.0 23.690363 3 ATCAACG 23280 0.0 22.876259 3 GAGTACT 13440 0.0 22.861906 12-13 TATCAAC 23365 0.0 22.856129 2 TCAACGC 23435 0.0 22.724955 4 CAACGCA 23610 0.0 22.576584 5 AACGCAG 24010 0.0 22.200464 6 AGTACTT 13685 0.0 21.708231 12-13 GTACTTT 14150 0.0 21.513865 14-15 CATGGGG 4840 0.0 19.970388 4 CATGGGA 7770 0.0 19.879171 4 ACGCAGA 26820 0.0 19.786135 7 CGCAGAG 26835 0.0 19.704449 8 AGAGTAC 22765 0.0 19.043865 10-11 >>END_MODULE