FastQCFastQC Report
Fri 27 May 2016
SRR939406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013269
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114430.5683790889344643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86570.42999718368484285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60580.3009036547028738No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23400.11622887949896413No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22780.1131493108968548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA175350.029.9680791
GGTATCA121600.028.8788281
GTACATG116450.027.2572121
TACATGG117600.026.4269732
TATCAAC210550.024.9184232
ACATGGG123350.024.7386133
ATCAACG217900.024.2532733
TCAACGC220950.024.1459354
GAGTACT107200.024.13605512-13
CAACGCA221700.023.811095
AACGCAG225000.023.2899366
CATGGGA70500.022.8578594
AGTACTT113700.021.99488412-13
GTACTTT120950.021.81278214-15
ACGCAGA244400.021.0248497
CGCAGAG243850.020.955028
GTATAGG6450.020.3092561
GTATACG1505.194516E-420.153031
GCAGAGT236150.020.0554899
TACCTGG12500.019.5767692
Produced by FastQC (version 0.11.5)