FastQCFastQC Report
Fri 27 May 2016
SRR939406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013269
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157060.7801242655601413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143460.7125724381590339No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98420.4888566803541901No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37900.18825104841926238No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36150.17955871768750226No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.14419334922456958No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28450.14131246246775767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128000.031.15371
GTATCAA185150.027.9987871
GTACATG135600.023.421071
TATCAAC226500.022.7972372
TACATGG136450.022.7818642
ATCAACG227850.022.658223
TCAACGC229900.022.4215894
CAACGCA232400.022.0756385
AACGCAG237700.021.5834166
ACATGGG137100.021.527763
GAGTACT132150.021.4858412-13
AGTACTT134100.021.26177612-13
GTACTTT139350.020.29065914-15
ACGACCG600.004800240519.76685348-49
ACGCAGA267750.019.0902427
AGCGTTG2001.305359E-518.966288
CGCAGAG269350.018.9592368
CATGGGG47750.018.687884
CATGGGA80400.018.419344
AGAGTAC227950.018.32287810-11
Produced by FastQC (version 0.11.5)