FastQCFastQC Report
Fri 27 May 2016
SRR939405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150640.46511814599905454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112510.34738743100340963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77150.2382094062920012No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42450.13106920670246858No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38030.11742195361354252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA255450.027.8271351
GTACATG169200.026.6865351
TACATGG177150.025.4082322
GAGTACT140600.024.08843812-13
GGTATCA181650.023.6724821
ACATGGG180850.023.5765463
TATCAAC303750.023.1918162
ATCAACG313300.022.4325373
AGTACTT149850.022.4256712-13
CAACGCA317450.022.2021885
TCAACGC318250.022.1264444
AACGCAG318150.021.9828556
GTACTTT161300.021.32151814-15
CATGGGG73800.020.3676364
CATGGGA103600.020.1924514
ACGCAGA342350.020.1134387
ATGGGAG41150.020.0743795
CGCAGAG342850.019.9928118
GCAGAGT340200.019.452849
TACTTTT175550.019.05108514-15
Produced by FastQC (version 0.11.5)