FastQCFastQC Report
Fri 27 May 2016
SRR939405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3238747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211070.6517026492035346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180410.5570364094509389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124850.3854885855548457No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68390.21116190921983102No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49590.1531147693845799No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44870.13854123214934666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43150.13323053637718538No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.1299885418651102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192300.028.5898251
GTATCAA264200.025.0967621
GTACATG178700.023.4720761
GAGTACT171900.022.68275312-13
TACATGG182700.022.5368542
ACATGGG184650.021.371783
GTACTTT186300.021.0057914-15
TATCAAC317950.020.9377292
ATCAACG321300.020.583523
TCAACGC325050.020.3897824
CAACGCA328600.020.1406675
AGTACTT178800.020.01883512-13
AACGCAG335350.019.777656
CATGGGA103950.019.4602494
TAAGGTG12950.019.3888075
ACTTTTT210150.018.97127716-17
GTAAGGT11050.018.8640694
TACTTTT198050.017.98932314-15
ACGCAGA370850.017.7822197
CGCAGAG372250.017.7153428
Produced by FastQC (version 0.11.5)