##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939405_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3238747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98092101667713 31.0 31.0 31.0 30.0 34.0 2 31.230642282339435 31.0 31.0 34.0 30.0 34.0 3 31.215233545565614 31.0 31.0 34.0 28.0 34.0 4 35.15485463977273 35.0 35.0 37.0 33.0 37.0 5 34.77421391667827 35.0 35.0 37.0 32.0 37.0 6 34.733497553220424 35.0 35.0 37.0 32.0 37.0 7 34.60209056156594 35.0 35.0 37.0 30.0 37.0 8 34.62485831712079 35.0 35.0 37.0 30.0 37.0 9 35.96028309713602 37.0 35.0 39.0 30.0 39.0 10-11 35.817635956127475 37.0 35.0 39.0 30.0 39.0 12-13 35.777965830612885 37.0 35.0 39.0 30.0 39.0 14-15 36.61270516035985 38.0 35.5 40.0 30.0 41.0 16-17 36.620790540292276 38.0 35.0 40.0 30.5 41.0 18-19 36.5972694069651 38.0 35.0 40.0 30.0 41.0 20-21 36.506914711152184 38.0 35.0 40.0 30.0 41.0 22-23 36.36203306402136 38.0 35.0 40.0 30.0 41.0 24-25 36.11987629783987 38.0 34.0 40.0 29.0 41.0 26-27 35.72331151522487 38.0 34.0 40.0 27.5 41.0 28-29 35.43483328583554 38.0 34.0 40.0 27.0 41.0 30-31 35.19304718769327 38.0 33.5 40.0 27.0 41.0 32-33 34.9663215434858 37.0 33.0 40.0 27.0 41.0 34-35 34.892193802109276 37.5 33.0 40.0 26.0 41.0 36-37 34.85239631252456 38.0 33.0 40.0 25.5 41.0 38-39 34.81036833071555 38.0 33.0 40.0 25.0 41.0 40-41 34.57584183018926 37.5 33.0 40.0 25.0 41.0 42-43 34.24787518135872 37.0 33.0 40.0 24.0 41.0 44-45 34.04184133555353 37.0 33.0 40.0 24.0 41.0 46-47 33.67075646847377 37.0 32.5 40.0 23.0 41.0 48-49 33.324637429228034 37.0 32.0 40.0 21.5 41.0 50-51 33.04067437190988 37.0 31.0 40.0 20.0 41.0 52-53 32.64586999231493 36.0 31.0 39.0 19.0 41.0 54-55 32.4073471932201 36.0 31.0 39.0 18.0 40.0 56-57 32.006880901780846 35.5 30.0 39.0 15.5 40.0 58-59 31.567996512231428 35.0 29.5 39.0 13.5 40.0 60-61 31.262145051774652 35.0 29.0 38.0 12.5 40.0 62-63 30.857903226155052 35.0 29.0 38.0 8.0 40.0 64-65 30.406025540124006 34.0 28.0 38.0 7.5 40.0 66-67 28.717632003981787 33.0 25.0 36.5 4.5 39.5 68-69 25.094685382958286 29.5 15.5 35.0 2.0 38.0 70-71 24.4889106805811 29.5 13.0 34.5 2.0 38.0 72-73 26.295566773199635 31.0 21.0 35.0 2.0 37.5 74-75 26.90179226719469 31.0 23.0 35.0 2.0 37.0 76-77 26.949519057833168 31.5 25.0 34.0 2.0 36.0 78-79 27.777982812488904 32.5 26.0 35.0 2.0 37.0 80-81 27.650194658613344 33.0 26.0 35.0 2.0 36.0 82-83 27.256883449062244 32.5 26.0 35.0 2.0 36.0 84-85 26.930911089998695 32.0 25.0 35.0 2.0 35.5 86-87 26.485399291763144 32.0 24.5 34.5 2.0 35.0 88-89 25.994177686617697 32.0 23.5 34.0 2.0 35.0 90-91 25.546476770183034 31.5 22.0 34.0 2.0 35.0 92-93 24.997875721691138 31.0 19.5 34.0 2.0 35.0 94-95 23.62939047106798 31.0 7.0 34.0 2.0 35.0 96-97 22.813594887158523 30.0 2.0 34.0 2.0 35.0 98-99 22.47582552758829 30.0 2.0 34.0 2.0 35.0 100-101 21.234097476585852 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4552869169336482 1101 2 0.28235765779195177 1101 3 0.35821920827149967 1101 4 0.3717084746803252 1101 5 0.41596575559145066 1101 6 0.4737444007313769 1101 7 0.5227303181693088 1101 8 0.5350134570741503 1101 9 0.5889535958340204 1101 10-11 0.8051917042825139 1101 12-13 0.6990898990561121 1101 14-15 0.7836120356634595 1101 16-17 0.7564069782944145 1101 18-19 0.8776490346089503 1101 20-21 0.7964386604714733 1101 22-23 0.7950070077235551 1101 24-25 0.7625489237003791 1101 26-27 0.8228291127132792 1101 28-29 0.7920312963402054 1101 30-31 0.789782396504151 1101 32-33 0.6914685499604616 1101 34-35 0.8947349520835317 1101 36-37 0.9227475766282822 1101 38-39 0.8181521458061383 1101 40-41 1.0395348589364488 1101 42-43 0.9758038141042107 1101 44-45 0.6732434059501884 1101 46-47 0.7244333205254065 1101 48-49 0.6402297838816722 1101 50-51 0.6438946185338068 1101 52-53 0.7679520052932176 1101 54-55 0.594295289853541 1101 56-57 0.558495245120028 1101 58-59 0.45745536627692474 1101 60-61 0.30546771419981766 1101 62-63 0.2458970421279254 1101 64-65 0.013357005283815226 1101 66-67 -2.852396897852387 1101 68-69 -7.860717682627861 1101 70-71 -11.139794698802177 1101 72-73 -11.137849666634565 1101 74-75 -9.649499206758232 1101 76-77 -4.690694334967436 1101 78-79 -1.5712177362291904 1101 80-81 -1.031194552129488 1101 82-83 -0.7402041407403281 1101 84-85 -0.785241230326367 1101 86-87 -0.6747300429266332 1101 88-89 -0.705030802684071 1101 90-91 -0.7534580666353143 1101 92-93 -1.3398420512588238 1101 94-95 -4.958806355143036 1101 96-97 -1.8335294095312307 1101 98-99 -1.187004747156081 1101 100-101 -0.9343355776422122 1102 1 0.4100320979652423 1102 2 0.3209069413522734 1102 3 0.4356780893641954 1102 4 0.23919361865809208 1102 5 0.44872168321823125 1102 6 0.5176029260679158 1102 7 0.4445360270208383 1102 8 0.4711233226554796 1102 9 0.6854487571561307 1102 10-11 0.6542663440917309 1102 12-13 0.6896074823779443 1102 14-15 0.5602176809519079 1102 16-17 0.6143764458427441 1102 18-19 0.652434016476235 1102 20-21 0.784804830623429 1102 22-23 0.7414212369842517 1102 24-25 0.7689295606122926 1102 26-27 0.6645558111779195 1102 28-29 0.6063812160240616 1102 30-31 0.3776925385109706 1102 32-33 0.3360379100921236 1102 34-35 0.5342796260886971 1102 36-37 0.7122364481618746 1102 38-39 0.6497845097614317 1102 40-41 0.8829236210824192 1102 42-43 0.9325544349528982 1102 44-45 0.6290799995603749 1102 46-47 0.5299207483394213 1102 48-49 0.3514720109546161 1102 50-51 0.5140383868882665 1102 52-53 0.5640983703719584 1102 54-55 0.3610421905374679 1102 56-57 0.23689531558142818 1102 58-59 0.244846717756122 1102 60-61 0.0925446378452861 1102 62-63 0.08467193766767167 1102 64-65 0.13507014116098404 1102 66-67 -2.7168252243202495 1102 68-69 -7.61410586580779 1102 70-71 -10.878405081757418 1102 72-73 -9.323325441217218 1102 74-75 -7.667007078232697 1102 76-77 -4.071102432115477 1102 78-79 -1.3443825370648739 1102 80-81 -0.6479144138674684 1102 82-83 -0.47336115633052955 1102 84-85 -0.44349790198609895 1102 86-87 -0.4150120363436649 1102 88-89 -0.42737562746677327 1102 90-91 -0.6471565140208497 1102 92-93 -1.1249049121887182 1102 94-95 -4.530631403924005 1102 96-97 -1.3851543947491152 1102 98-99 -0.8339219993586156 1102 100-101 -0.7904754509102467 1103 1 0.33568223682926757 1103 2 0.2402811339329638 1103 3 0.3444141545770627 1103 4 0.2570344100862627 1103 5 0.4723421755807138 1103 6 0.4137742465341532 1103 7 0.43065431183240577 1103 8 0.40780637761563554 1103 9 0.5960060518720383 1103 10-11 0.5731176797507445 1103 12-13 0.528800628567403 1103 14-15 0.7767958893398799 1103 16-17 0.5989502766270149 1103 18-19 0.5801399749283078 1103 20-21 0.7074728857287411 1103 22-23 0.685252523242518 1103 24-25 0.8760874839661312 1103 26-27 0.7714705259260484 1103 28-29 0.7280333743435605 1103 30-31 0.7184123724789515 1103 32-33 0.7441538198208306 1103 34-35 0.8058563950325137 1103 36-37 0.6547875816939097 1103 38-39 0.721002672643607 1103 40-41 0.7597461358034607 1103 42-43 0.7687721502768099 1103 44-45 0.5720854198538703 1103 46-47 0.688352670493316 1103 48-49 0.6596646406376507 1103 50-51 0.5786156771769768 1103 52-53 0.6391259949987926 1103 54-55 0.6448437262671334 1103 56-57 0.717396366133741 1103 58-59 0.7464984496856566 1103 60-61 0.6292287663404714 1103 62-63 0.7041126138193476 1103 64-65 0.4861742341418207 1103 66-67 -2.873070839458851 1103 68-69 -7.762970851993963 1103 70-71 -11.219993345086186 1103 72-73 -9.255399175232128 1103 74-75 -7.38835193336897 1103 76-77 -3.687216510484138 1103 78-79 -0.838023580819133 1103 80-81 -0.24494859945657765 1103 82-83 0.08872878352731917 1103 84-85 -0.011445260666551604 1103 86-87 0.2242614435307253 1103 88-89 0.3128759940247363 1103 90-91 0.25619547059766035 1103 92-93 0.29345070732869516 1103 94-95 1.1537952618394804 1103 96-97 1.3595791599037952 1103 98-99 1.1097912736470867 1103 100-101 1.0243631922382441 1104 1 0.3842414298285064 1104 2 0.24033079727253792 1104 3 0.33901810519819975 1104 4 0.14163811464130305 1104 5 0.5052353606902287 1104 6 0.5447150172251582 1104 7 0.46927453522440743 1104 8 0.39847177034012304 1104 9 0.587537777293484 1104 10-11 0.6522828435137384 1104 12-13 0.6470294598314865 1104 14-15 0.7496145134223084 1104 16-17 0.7132277215487264 1104 18-19 0.7724575064172683 1104 20-21 0.8476240303261804 1104 22-23 0.7363507732324663 1104 24-25 0.6753023530799709 1104 26-27 0.9056085053873346 1104 28-29 0.9759848741164063 1104 30-31 0.9448994819165506 1104 32-33 0.7267967827206618 1104 34-35 0.8505397469808358 1104 36-37 0.8946096729076132 1104 38-39 0.8882159111194596 1104 40-41 0.8456325048821896 1104 42-43 1.004889671443003 1104 44-45 0.7677122402178185 1104 46-47 0.6628870804358229 1104 48-49 0.737333452480506 1104 50-51 0.6948330681682009 1104 52-53 0.7645559354808071 1104 54-55 0.6168318022643433 1104 56-57 0.7241204972353472 1104 58-59 0.886091144283423 1104 60-61 0.6549194168492001 1104 62-63 0.7960539342580155 1104 64-65 0.5738898945682074 1104 66-67 -2.5914220886043715 1104 68-69 -7.285629488103623 1104 70-71 -8.247373224321748 1104 72-73 -7.791496996106815 1104 74-75 -6.626928314985076 1104 76-77 -3.3383445284697686 1104 78-79 -0.4605074799944546 1104 80-81 0.25920211542543825 1104 82-83 0.37810701051908424 1104 84-85 0.3992479015168122 1104 86-87 0.5468819243918865 1104 88-89 0.4893533208347165 1104 90-91 0.3897921796737194 1104 92-93 0.3734201621242548 1104 94-95 1.2977408506246313 1104 96-97 1.4141934543032697 1104 98-99 1.0727470735749485 1104 100-101 1.0575724372414506 1105 1 0.5313756122163191 1105 2 0.4038856543134628 1105 3 0.4842508037193234 1105 4 0.301796845089946 1105 5 0.4275926283980951 1105 6 0.5430766939768574 1105 7 0.48001785057941504 1105 8 0.5090107975620981 1105 9 0.7719699926584127 1105 10-11 0.8098431482382082 1105 12-13 0.8296473280874039 1105 14-15 0.982157101607747 1105 16-17 1.0148286635084887 1105 18-19 0.9938525255736579 1105 20-21 1.0238495612591905 1105 22-23 1.0525670227690043 1105 24-25 1.1081536260833218 1105 26-27 1.090017737956785 1105 28-29 1.1192827561072036 1105 30-31 1.0065549402848148 1105 32-33 1.0946817049718405 1105 34-35 1.1847875216069852 1105 36-37 1.1427522520825804 1105 38-39 1.0238689695359469 1105 40-41 1.1387065253128341 1105 42-43 1.0149578101044412 1105 44-45 1.0245245082209635 1105 46-47 1.0640870867675147 1105 48-49 0.9618456845026415 1105 50-51 1.1923111249256522 1105 52-53 1.4587811047772519 1105 54-55 1.358516900828569 1105 56-57 1.2761108529846723 1105 58-59 1.3914184345113831 1105 60-61 1.3689496676873851 1105 62-63 1.4611995508148823 1105 64-65 1.4827090781097283 1105 66-67 2.0449637556983014 1105 68-69 -4.811789084288296 1105 70-71 -5.203535825012267 1105 72-73 -1.3720718612693439 1105 74-75 1.358412162192554 1105 76-77 1.3497269592872492 1105 78-79 1.2198516056373094 1105 80-81 1.270630805138218 1105 82-83 1.3667317443904885 1105 84-85 1.241039207992845 1105 86-87 1.3969440692620374 1105 88-89 1.4737454913420969 1105 90-91 1.3751633259459695 1105 92-93 1.4810002685817807 1105 94-95 2.3560962230889864 1105 96-97 2.507759839270875 1105 98-99 2.1815876569909314 1105 100-101 1.9978350529697195 1106 1 0.15765831196185331 1106 2 0.1670001018105296 1106 3 0.09831964063380738 1106 4 0.06795853942368524 1106 5 0.3185233031870709 1106 6 0.3757821516581288 1106 7 0.45338956586383006 1106 8 0.3164303253701277 1106 9 0.49092999472215837 1106 10-11 0.483198786589206 1106 12-13 0.39768983936890123 1106 14-15 0.510911318923668 1106 16-17 0.30709856067139185 1106 18-19 0.43224078766175467 1106 20-21 0.4939128371209449 1106 22-23 0.4105786792367283 1106 24-25 0.5532863690384886 1106 26-27 0.7412685141640267 1106 28-29 0.8372253969254828 1106 30-31 0.7785427955205506 1106 32-33 0.7143235717018968 1106 34-35 0.8930261899025354 1106 36-37 0.8446495144894541 1106 38-39 0.7591088244842297 1106 40-41 0.7631399170574866 1106 42-43 0.8801066576324672 1106 44-45 0.8546307081129854 1106 46-47 0.7071858442831314 1106 48-49 0.6479014092729045 1106 50-51 0.5129975777368614 1106 52-53 0.6797656948753357 1106 54-55 0.807348259785492 1106 56-57 0.7551302245378082 1106 58-59 0.9489206502721181 1106 60-61 0.9338753729073694 1106 62-63 1.001228811755027 1106 64-65 0.8783244054618891 1106 66-67 1.5542169752309043 1106 68-69 -5.045760980518832 1106 70-71 -5.988750758410461 1106 72-73 -2.1914627159357067 1106 74-75 0.7248989883202874 1106 76-77 0.9500504787813391 1106 78-79 1.014823256798259 1106 80-81 1.0430350944087792 1106 82-83 1.0376438270581723 1106 84-85 0.9716671942026132 1106 86-87 1.092813402949151 1106 88-89 1.0965106133333151 1106 90-91 0.8515129591707726 1106 92-93 1.1173867912286042 1106 94-95 2.0099045676162888 1106 96-97 2.0913792406745912 1106 98-99 1.7820445367207292 1106 100-101 1.638915210993538 1107 1 -0.03889037082862146 1107 2 -0.03921525610019572 1107 3 -0.02499187305583561 1107 4 -0.002366134620146454 1107 5 0.06995101986420593 1107 6 0.09427282030857498 1107 7 0.09327362963392005 1107 8 0.0789592436774953 1107 9 0.016623494008769057 1107 10-11 0.08618001399347719 1107 12-13 0.1306510505610703 1107 14-15 0.10262849576422184 1107 16-17 -0.014802941906481237 1107 18-19 0.1688534781844453 1107 20-21 0.12683560798087257 1107 22-23 0.1705799754531867 1107 24-25 0.3012059651073855 1107 26-27 0.3037851278079273 1107 28-29 0.2814536878828662 1107 30-31 0.2317934105189039 1107 32-33 0.26016548777281656 1107 34-35 0.26852577332862637 1107 36-37 0.22384965500683762 1107 38-39 0.1764295873357966 1107 40-41 0.27449554813173904 1107 42-43 0.4970604879051308 1107 44-45 0.3253581720279186 1107 46-47 0.24537456494566356 1107 48-49 0.4206009303303162 1107 50-51 0.3749026214278146 1107 52-53 0.5491803404305813 1107 54-55 0.6121055385575076 1107 56-57 0.5041270657146555 1107 58-59 0.4435029586082031 1107 60-61 0.3074190079679582 1107 62-63 0.5062983800679817 1107 64-65 0.6099865823353205 1107 66-67 1.3862529179982488 1107 68-69 -5.361909348460205 1107 70-71 -6.112990757583887 1107 72-73 -2.251600467112869 1107 74-75 0.3731478750300319 1107 76-77 0.4866400628367522 1107 78-79 0.5404531193711364 1107 80-81 0.42934458299502865 1107 82-83 0.5545756545261291 1107 84-85 0.6058751448937869 1107 86-87 0.7709841080815139 1107 88-89 0.645453821206047 1107 90-91 0.5796131238278335 1107 92-93 0.5527493843002347 1107 94-95 1.4333817715834627 1107 96-97 1.439090714162738 1107 98-99 1.120652437817533 1107 100-101 0.9151554928924384 1108 1 0.063116789325111 1108 2 0.04611300819901487 1108 3 0.07077887806422112 1108 4 0.052497148780908276 1108 5 0.17426694579490487 1108 6 0.1445784069734657 1108 7 0.2018983776006138 1108 8 0.01232876396436211 1108 9 0.20168662570115004 1108 10-11 0.29556981327746 1108 12-13 0.24486475192382073 1108 14-15 0.27027349745915075 1108 16-17 0.12305492870823542 1108 18-19 0.21584037793513744 1108 20-21 0.2477257784314375 1108 22-23 0.21400467726839167 1108 24-25 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64-65 -0.5317542067663581 2314 66-67 -0.4547735242736728 2314 68-69 1.6992456129998494 2314 70-71 1.6128363306785403 2314 72-73 -0.3499305151575989 2314 74-75 0.14350067968532798 2314 76-77 -0.03546222193848436 2314 78-79 0.4194303413701519 2314 80-81 0.6859871784706755 2314 82-83 0.29288061998525805 2314 84-85 0.3655452396068206 2314 86-87 0.1508120059246174 2314 88-89 0.4523620879500925 2314 90-91 1.0559539307858543 2314 92-93 1.078285586181103 2314 94-95 0.9023928741384104 2314 96-97 1.2881142650086836 2314 98-99 0.6884274999427369 2314 100-101 0.6533505999747113 2315 1 -0.3244879214606158 2315 2 -0.2630959045758914 2315 3 -0.349471072046299 2315 4 -0.178890266273676 2315 5 -1.0510210041053938 2315 6 -0.32361266897888896 2315 7 -0.21732788605044817 2315 8 -0.10148856588966026 2315 9 -0.07490370625050957 2315 10-11 -1.7577951751222898 2315 12-13 -1.282834954527992 2315 14-15 -0.24709946716426145 2315 16-17 -1.736976893925899 2315 18-19 -2.8446070126545635 2315 20-21 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-5.46613981532057 2315 88-89 -4.045318639953681 2315 90-91 -3.841354762695815 2315 92-93 -4.443671724949027 2315 94-95 -2.54044026307454 2315 96-97 -1.5239567216551322 2315 98-99 -3.0253942325728325 2315 100-101 -2.3580435113622364 2316 1 -0.37528715646167754 2316 2 -0.37643476465052217 2316 3 -0.8384035977058559 2316 4 -0.32720456645443363 2316 5 -0.36766873818518775 2316 6 -2.2016356521774227 2316 7 -0.367247302314631 2316 8 -0.504459276784246 2316 9 -0.21178543486127666 2316 10-11 -0.19058208688944234 2316 12-13 -0.6499515871334935 2316 14-15 -1.044767610835052 2316 16-17 -1.5132818213830532 2316 18-19 -0.29768830209927444 2316 20-21 -4.100591445152084 2316 22-23 -4.297197326097759 2316 24-25 -2.963400103609956 2316 26-27 -2.349754578699489 2316 28-29 -1.4635327245990908 2316 30-31 -0.35920861180299823 2316 32-33 -1.825251406401641 2316 34-35 -2.3050824058399613 2316 36-37 -4.551220335285251 2316 38-39 -2.1614318010953397 2316 40-41 -1.6430983239783643 2316 42-43 -3.07871445495449 2316 44-45 -0.8076096298376356 2316 46-47 -0.9238103563989029 2316 48-49 -0.4340278166375029 2316 50-51 -0.12972663187314026 2316 52-53 -2.9879245041922005 2316 54-55 -1.3146403733498317 2316 56-57 -0.29202998897014254 2316 58-59 -0.15528414089939346 2316 60-61 -1.4546173516774559 2316 62-63 -0.9818436400807897 2316 64-65 0.1248237852854075 2316 66-67 1.365079229585806 2316 68-69 3.6990203174885643 2316 70-71 3.9602559407819697 2316 72-73 2.2099095250293885 2316 74-75 1.23162605385102 2316 76-77 0.04466317133353925 2316 78-79 0.4246829907816796 2316 80-81 -0.11428456931298925 2316 82-83 0.07510205760945254 2316 84-85 0.3531269191808768 2316 86-87 -0.06185322405194782 2316 88-89 0.5997913308297953 2316 90-91 0.8720729887162619 2316 92-93 0.9131312388534383 2316 94-95 1.338764804387555 2316 96-97 1.2787143471659164 2316 98-99 0.10403164219575345 2316 100-101 0.02312083835806078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 57.0 4 124.0 5 280.0 6 777.0 7 1742.0 8 3657.0 9 6119.0 10 9527.0 11 13763.0 12 17860.0 13 22347.0 14 27031.0 15 33738.0 16 39896.0 17 44954.0 18 41631.0 19 35477.0 20 34668.0 21 35091.0 22 37156.0 23 42402.0 24 50995.0 25 63330.0 26 79060.0 27 97279.0 28 118837.0 29 141269.0 30 165952.0 31 192579.0 32 222642.0 33 257998.0 34 299310.0 35 342289.0 36 353797.0 37 288016.0 38 111399.0 39 5690.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.07228641806938 20.194823484067985 15.30502704131676 22.427863056545878 2 16.21926269060027 21.782389500096507 37.649256900212315 24.349090909090908 3 19.262480212254925 25.452003506294258 30.441216927410508 24.84429935404031 4 13.277727466825906 17.72691723064506 36.26797647361773 32.7273788289113 5 13.717072508023943 38.16382580289587 33.13561271418404 14.983488974896138 6 31.516601945134955 36.40997583324662 16.910274251122427 15.163147970495997 7 28.078358698595473 33.94575124268737 20.078567421289776 17.897322637427376 8 27.059106500137244 33.79164843688007 19.462032693507705 19.687212369474985 9 26.82747371128402 16.67956774641551 20.18836296876539 36.304595573535074 10-11 25.263643625142684 26.825667457198726 26.314157913538782 21.596531004119804 12-13 26.37893605150387 24.296093520117502 27.38647075551131 21.938499672867316 14-15 23.322414501657583 25.489348195459538 26.227473155513536 24.96076414736934 16-17 22.089533160035113 28.96550904319683 26.708592526768953 22.2363652699991 18-19 21.7230739684306 26.852869633736436 29.935227217289867 21.488829180543092 20-21 22.663783658276433 25.905500785566264 29.785396912046064 21.645318644111235 22-23 22.28659725504956 25.83639598894264 29.79996585099114 22.077040905016663 24-25 22.388195187830355 26.138874076919254 29.797295065035957 21.675635670214437 26-27 22.169464909002127 26.380082066131177 29.809497829318566 21.64095519554813 28-29 21.65557494248259 26.41522165433967 30.31656964624863 21.61263375692911 30-31 21.781384268844146 26.36020755850127 29.988004094355443 21.870404078299146 32-33 21.687895266818323 26.522714226471273 29.60516625561954 22.184224251090868 34-35 22.469918414497336 26.319422636124827 29.413965291345257 21.796693658032577 36-37 22.478551932033803 26.098598214178086 29.50112707251992 21.921722781268187 38-39 22.144355567314715 26.260554266478287 29.71931840696219 21.875771759244806 40-41 22.05579512779164 26.243219669492845 30.000542534638363 21.700442668077155 42-43 22.15466723868863 26.584092328796 29.150247565377875 22.110992867137494 44-45 21.99206488534047 26.83591861763219 28.9423988685492 22.22961762847814 46-47 22.177299649955255 26.959812464659016 28.388766485954708 22.47412139943102 48-49 22.46338803016305 26.939443009084957 28.224976656305085 22.37219230444691 50-51 22.470214339306562 27.125176658581644 28.073156163481727 22.331452838630064 52-53 22.735141881518224 27.071581717701044 27.96348521407337 22.229791186707367 54-55 22.030060773167424 26.92358375532416 28.26479854202485 22.781556929483568 56-57 22.312571805318726 27.556377455164675 27.73050945540622 22.40054128411037 58-59 22.508704270060353 26.828313609727843 27.87256352623263 22.790418593979172 60-61 22.375596563227635 26.842755266377033 28.39039594768539 22.39125222270994 62-63 22.510901039373422 26.89887429516081 28.204667824750935 22.385556840714834 64-65 22.266962269015636 26.76287637965346 28.23606605971435 22.734095291616555 66-67 21.658913159113386 27.024794118755096 28.73886526601737 22.577427456114144 68-69 21.584591439688715 26.686817120622568 29.21649805447471 22.51209338521401 70-71 21.264163420578523 26.766273085781627 29.366851189349557 22.60271230429029 72-73 22.059012046282696 26.89269754491656 28.037425717067176 23.01086469173357 74-75 21.813981930221 27.351927770360586 27.96737018772501 22.866720111693407 76-77 22.175377603993766 27.109991918466996 27.8354770343337 22.879153443205535 78-79 22.06008411405421 27.114106705038814 27.924816116302193 22.90099306460478 80-81 21.86556334331676 27.778661191218703 27.613162688733134 22.742612776731406 82-83 22.14777813765632 27.45722342622008 27.424139644127806 22.970858791995795 84-85 21.96633451146385 27.19531658385172 27.359330630024516 23.47901827465992 86-87 22.202104646933467 27.13113873366725 27.538936462288703 23.12782015711058 88-89 22.042740998982005 27.017859232529425 27.47005990335062 23.469339865137954 90-91 21.819936766560105 27.203671794393593 27.77198229685999 23.204409142186314 92-93 21.953185076865196 27.22889921988508 27.57691035216559 23.241005351084134 94-95 21.489304188534575 26.984087687535098 27.850546592629232 23.676061531301094 96-97 21.43464146342218 26.81292223325999 27.840595049097434 23.9118412542204 98-99 21.717650989737464 26.86838982263132 27.26072736694106 24.153231820690163 100-101 21.69859357336488 26.66561370712471 27.609170443888836 24.026622275621577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5010.0 1 3342.5 2 1886.5 3 2313.5 4 2286.0 5 2300.0 6 3695.5 7 6407.0 8 9270.0 9 10694.5 10 9573.5 11 7765.5 12 6845.0 13 6426.5 14 6384.0 15 6279.0 16 6138.0 17 6326.0 18 6624.5 19 6906.5 20 7340.5 21 8107.5 22 9115.5 23 10405.0 24 12087.5 25 14366.5 26 17477.5 27 20927.5 28 24596.5 29 29340.5 30 34487.0 31 39834.5 32 46717.0 33 52275.0 34 56440.5 35 61509.5 36 68242.5 37 74816.0 38 78020.5 39 79005.5 40 80768.0 41 87038.0 42 94208.5 43 99280.0 44 103274.5 45 106888.5 46 110929.5 47 119024.0 48 138151.5 49 141571.0 50 127844.0 51 120567.0 52 115995.5 53 114635.0 54 112097.5 55 103961.5 56 94024.5 57 84764.5 58 76075.0 59 67491.0 60 58449.5 61 48721.0 62 40076.0 63 32083.5 64 24758.0 65 19184.0 66 14567.5 67 11551.5 68 8393.0 69 5483.0 70 4022.0 71 3147.0 72 2683.5 73 2351.0 74 1819.0 75 1366.0 76 909.0 77 534.5 78 337.5 79 225.5 80 156.0 81 95.5 82 68.5 83 40.0 84 20.5 85 15.0 86 8.0 87 5.5 88 2.5 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02281746613736732 2 0.019204957966769246 3 0.0 4 0.0 5 6.175227642047989E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.3226593494335926E-4 18-19 0.0012659216666198378 20-21 1.5438069105119973E-5 22-23 0.0 24-25 0.0 26-27 5.403324186791991E-4 28-29 0.01843305451151325 30-31 0.004384411625854073 32-33 0.008336557316764786 34-35 0.009386346015912943 36-37 0.10417609032134958 38-39 0.10633741999606638 40-41 0.12135866123534811 42-43 0.10621391544322542 44-45 0.10050182987433104 46-47 0.10199932257752765 48-49 0.10491711763839534 50-51 0.10311086355309632 52-53 0.07961412237510371 54-55 0.06803557054626372 56-57 0.03891937221400745 58-59 0.016595924288003973 60-61 0.009309155670387344 62-63 0.0020069489836655966 64-65 0.005001934390058871 66-67 0.42138209622424966 68-69 0.8104059996041678 70-71 0.898943325922031 72-73 0.28415309994883825 74-75 0.15112325847001942 76-77 0.015438069105119974 78-79 0.008861451666338865 80-81 0.0014511784958812775 82-83 0.0 84-85 0.0 86-87 2.778852438921595E-4 88-89 9.262841463071984E-4 90-91 0.003473565548651994 92-93 0.0017753779470887968 94-95 0.22596701749164105 96-97 0.2290083171053497 98-99 0.014326528129551337 100-101 0.007904291381821426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3238747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.488257106261905 #Duplication Level Percentage of deduplicated Percentage of total 1 64.13422554962702 20.83609208967274 2 14.208843160329417 9.232410995506662 3 6.665554256061757 6.496567212800179 4 3.840943494393609 4.991422391059344 5 2.519882636697452 4.093329748931599 6 1.745662663088355 3.40281224515298 7 1.214976667804579 2.763073205322117 8 0.909561995989642 2.3640067183837004 9 0.7014562518766564 2.051018195378726 >10 3.6357083175682314 20.804481203105478 >50 0.24201482459130752 5.465474186261026 >100 0.1634564553442007 10.161901364663022 >500 0.012873910609329992 2.8415589988640924 >1k 0.004452630737003512 2.657056883427666 >5k 9.679632036964158E-5 0.21506902459122532 >10k+ 2.903889611089247E-4 1.623725536879476 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21107 0.6517026492035346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18041 0.5570364094509389 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12485 0.3854885855548457 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6839 0.21116190921983102 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4959 0.1531147693845799 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4487 0.13854123214934666 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4315 0.13323053637718538 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.1299885418651102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.631420731535992E-5 0.0 12-13 0.0 0.0 0.0 1.0806648373583981E-4 0.0 14-15 0.0 0.0 0.0 1.852568292614397E-4 0.0 16-17 1.5438069105119973E-5 0.0 0.0 4.168278658382393E-4 0.0 18-19 3.0876138210239945E-5 0.0 0.0 5.094562804689592E-4 0.0 20-21 3.0876138210239945E-5 0.0 0.0 7.255892479406387E-4 0.0 22-23 3.0876138210239945E-5 0.0 0.0 0.001682749532458077 0.0 24-25 6.175227642047989E-5 0.0 0.0 0.0030567376828137547 0.0 26-27 6.175227642047989E-5 0.0 0.0 0.004384411625854073 0.0 28-29 9.262841463071985E-5 0.0 0.0 0.007672720345244627 0.0 30-31 9.262841463071985E-5 0.0 0.0 0.014619851442548615 0.0 32-33 9.262841463071985E-5 0.0 0.0 0.026538040791701238 0.0 34-35 1.0806648373583981E-4 0.0 0.0 0.04291783211223353 0.0 36-37 1.5438069105119974E-4 0.0 0.0 0.07055197581039828 0.0 38-39 1.852568292614397E-4 0.0 0.0 0.10860681615451902 0.0 40-41 1.852568292614397E-4 0.0 0.0 0.15098431584807334 0.0 42-43 1.852568292614397E-4 0.0 0.0 0.19490562245213966 0.0 44-45 1.852568292614397E-4 0.0 0.0 0.24907780694200565 0.0 46-47 2.0069489836655965E-4 0.0 0.0 0.3156776370614932 0.0 48-49 2.1613296747167963E-4 0.0 0.0 0.3870169543962526 0.0 50-51 2.1613296747167963E-4 0.0 0.0 0.4666002006331461 0.0 52-53 2.1613296747167963E-4 0.0 0.0 0.5445315734757916 0.0 54-55 2.1613296747167963E-4 0.0 0.0 0.6261372067654559 0.0 56-57 2.1613296747167963E-4 0.0 0.0 0.71995435271727 0.0 58-59 2.1613296747167963E-4 0.0 0.0 0.8155314385470678 0.0 60-61 2.315710365767996E-4 0.0 0.0 0.9170984951896521 0.0 62-63 2.4700910568191956E-4 0.0 0.0 1.0173533159583013 0.0 64-65 2.4700910568191956E-4 0.0 0.0 1.121313273312179 0.0 66-67 2.4700910568191956E-4 0.0 0.0 1.2299509656049084 0.0 68-69 2.4700910568191956E-4 0.0 0.0 1.3629190548073067 0.0 70-71 2.4700910568191956E-4 0.0 0.0 1.5373537976260572 0.0 72-73 2.4700910568191956E-4 0.0 0.0 1.7291100539807525 0.0 74-75 2.4700910568191956E-4 0.0 0.0 1.9382187000096025 0.0 76-77 2.4700910568191956E-4 0.0 0.0 2.1725840270944285 0.0 78-79 2.4700910568191956E-4 0.0 0.0 2.434398241048158 0.0 80-81 2.778852438921595E-4 0.0 0.0 2.723661341870791 0.0 82-83 2.778852438921595E-4 0.0 0.0 3.023607586514167 0.0 84-85 2.778852438921595E-4 0.0 0.0 3.3310412946735264 0.0 86-87 3.087613821023995E-4 0.0 0.0 3.6482164244382167 0.0 88-89 3.3963752031263944E-4 0.0 0.0 3.989135304486581 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19230 0.0 28.589825 1 GTATCAA 26420 0.0 25.096762 1 GTACATG 17870 0.0 23.472076 1 GAGTACT 17190 0.0 22.682753 12-13 TACATGG 18270 0.0 22.536854 2 ACATGGG 18465 0.0 21.37178 3 GTACTTT 18630 0.0 21.00579 14-15 TATCAAC 31795 0.0 20.937729 2 ATCAACG 32130 0.0 20.58352 3 TCAACGC 32505 0.0 20.389782 4 CAACGCA 32860 0.0 20.140667 5 AGTACTT 17880 0.0 20.018835 12-13 AACGCAG 33535 0.0 19.77765 6 CATGGGA 10395 0.0 19.460249 4 TAAGGTG 1295 0.0 19.388807 5 ACTTTTT 21015 0.0 18.971277 16-17 GTAAGGT 1105 0.0 18.864069 4 TACTTTT 19805 0.0 17.989323 14-15 ACGCAGA 37085 0.0 17.782219 7 CGCAGAG 37225 0.0 17.715342 8 >>END_MODULE