FastQCFastQC Report
Fri 27 May 2016
SRR939404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227307
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150590.46661194612102286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105830.32792046123904545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76890.23824817409685536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44880.13906331191919455No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35300.10937912011469626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA240800.028.1871721
GTACATG162450.026.481491
TACATGG166300.025.0595932
GGTATCA171600.024.424391
GAGTACT134300.023.37527712-13
TATCAAC289450.023.151212
ACATGGG177850.022.995413
ATCAACG301850.022.4592843
AGTACTT141350.022.43048112-13
TCAACGC308150.022.1095244
CAACGCA306950.022.060035
AACGCAG312150.021.6421326
GTACTTT151350.021.08292614-15
CATGGGA101100.020.3252284
ACGCAGA333950.019.9095467
CGCAGAG338250.019.613128
ACTTTTT185550.019.26983516-17
TACTTTT167600.019.23696314-15
ATGGGAG38750.019.165465
CATGGGG74450.019.1476634
Produced by FastQC (version 0.11.5)