FastQCFastQC Report
Fri 27 May 2016
SRR939404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3227307
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211340.6548493837121786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174190.5397379301070521No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124540.3858944934584779No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69440.21516391220296055No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA47960.14860687254110005No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42930.13302112256441673No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42890.1328971802186777No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41090.12731977466042121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195250.025.4763011
GTATCAA267950.025.1713491
GTACATG184500.023.076091
TACATGG187150.022.2612512
GAGTACT158800.021.9417412-13
ACATGGG186600.021.3572433
AGTACTT165050.020.82364512-13
TATCAAC325750.020.6118472
ATCAACG326600.020.5259633
GTACTTT170600.020.45187214-15
TCAACGC331100.020.2217854
CAACGCA333750.020.0589525
AACGCAG337400.019.8560076
TACTTTT182900.018.6617814-15
ACTTTTT195250.018.47670416-17
CCCTATA4400.018.333322
CATGGGG77200.018.193914
ACGCAGA374250.017.8375687
CATGGGA106000.017.7719974
CGCAGAG374900.017.7687028
Produced by FastQC (version 0.11.5)