##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3227307 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.87949302622899 31.0 31.0 31.0 28.0 34.0 2 31.116591635069113 31.0 31.0 34.0 28.0 34.0 3 31.116296652286255 31.0 31.0 34.0 28.0 34.0 4 35.03439895863641 35.0 35.0 37.0 32.0 37.0 5 34.598569333503136 35.0 35.0 37.0 30.0 37.0 6 34.66128292102363 35.0 35.0 37.0 31.0 37.0 7 34.503545525727795 35.0 35.0 37.0 30.0 37.0 8 34.405174345049915 35.0 35.0 37.0 30.0 37.0 9 35.890883327802406 37.0 35.0 39.0 30.0 39.0 10-11 35.68513531560524 37.0 35.0 39.0 30.0 39.0 12-13 35.66651282323002 37.0 35.0 39.0 30.0 39.0 14-15 36.46148940897163 38.0 35.0 40.0 30.0 41.0 16-17 36.38106833344333 38.0 35.0 40.0 30.0 41.0 18-19 36.39382246560368 38.0 35.0 40.0 30.0 41.0 20-21 36.28555200977162 38.0 34.5 40.0 29.5 41.0 22-23 36.11745086538095 38.0 34.0 40.0 29.0 41.0 24-25 35.928666377261294 38.0 34.0 40.0 29.0 41.0 26-27 35.43079694618454 38.0 34.0 40.0 27.0 41.0 28-29 35.20894835849208 38.0 33.5 40.0 27.0 41.0 30-31 34.89161226372328 37.0 33.0 40.0 26.5 41.0 32-33 34.62420789221478 37.0 33.0 40.0 25.0 41.0 34-35 34.83912732814077 37.0 33.0 40.0 26.0 41.0 36-37 34.65382453544085 37.0 33.0 40.0 25.0 41.0 38-39 34.44082264253137 37.0 33.0 40.0 25.0 41.0 40-41 34.26275777296675 37.0 33.0 40.0 24.5 41.0 42-43 34.077012506092544 37.0 33.0 40.0 24.0 41.0 44-45 33.660623547744294 37.0 32.0 40.0 23.0 41.0 46-47 33.20739427640444 37.0 31.0 40.0 21.5 41.0 48-49 32.77625989718362 36.0 31.0 39.0 19.5 40.5 50-51 32.50870880892336 36.0 31.0 39.0 18.0 40.0 52-53 32.371457224243 36.0 31.0 39.0 18.0 40.0 54-55 32.01446546609913 35.5 30.5 39.0 15.5 40.0 56-57 31.51173749506942 35.0 29.5 39.0 13.0 40.0 58-59 31.03946200345985 35.0 29.0 38.0 9.5 40.0 60-61 30.657897280921834 34.0 28.0 38.0 8.0 40.0 62-63 30.470740000873796 34.0 28.0 38.0 8.0 40.0 64-65 29.996147252182702 34.0 28.0 37.5 7.0 40.0 66-67 29.12499508103815 33.5 27.0 36.5 2.0 39.0 68-69 25.725618294138116 31.0 16.5 35.0 2.0 38.5 70-71 24.4282643392773 29.5 12.0 35.0 2.0 38.0 72-73 25.415826569954454 30.0 18.0 35.0 2.0 37.0 74-75 26.15173378299616 31.0 21.0 35.0 2.0 37.0 76-77 25.80960488109746 30.5 21.5 34.0 2.0 36.0 78-79 26.910170925790453 32.0 25.0 35.0 2.0 36.0 80-81 26.941835251496062 32.0 25.0 35.0 2.0 36.0 82-83 26.622165787140794 32.0 24.5 35.0 2.0 36.0 84-85 26.270230102063422 32.0 24.0 34.0 2.0 35.0 86-87 25.82316045545094 31.5 23.0 34.0 2.0 35.0 88-89 25.339002301299505 31.0 20.5 34.0 2.0 35.0 90-91 24.74667919723782 31.0 19.0 34.0 2.0 35.0 92-93 24.19317421614987 31.0 17.0 34.0 2.0 35.0 94-95 23.351565872103272 30.0 7.0 34.0 2.0 35.0 96-97 22.67684140368425 30.0 2.0 34.0 2.0 35.0 98-99 21.82457959530965 29.5 2.0 34.0 2.0 35.0 100-101 20.42822095945629 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28134552948619884 1101 2 0.20652607399601308 1101 3 0.3369131741761535 1101 4 0.366735279201194 1101 5 0.5338662172153548 1101 6 0.40602561488532984 1101 7 0.5325581234620884 1101 8 0.7397334531019837 1101 9 0.7303375054081158 1101 10-11 0.7844469144612702 1101 12-13 0.8019798525421109 1101 14-15 0.6992581614973616 1101 16-17 0.715570980361889 1101 18-19 0.5431828003289993 1101 20-21 0.6081083903095177 1101 22-23 0.7578104769511711 1101 24-25 0.7326285560828367 1101 26-27 0.7090666016303047 1101 28-29 0.6601824991529242 1101 30-31 0.3732092455930527 1101 32-33 0.8175332801266606 1101 34-35 0.7871297246359887 1101 36-37 1.0118614133184636 1101 38-39 1.0185675587002194 1101 40-41 0.9374869934695553 1101 42-43 0.6799921391854937 1101 44-45 0.7331120080865929 1101 46-47 0.8116223128488187 1101 48-49 0.7944540699338845 1101 50-51 0.556720865176267 1101 52-53 0.39691729105128104 1101 54-55 0.45066800434415555 1101 56-57 0.6284860290386867 1101 58-59 0.6747103160699375 1101 60-61 0.5014225920423208 1101 62-63 0.37195906251862354 1101 64-65 0.3463708815402242 1101 66-67 0.28449933113159176 1101 68-69 -6.578317428838638 1101 70-71 -7.490728716930196 1101 72-73 -4.780810166679242 1101 74-75 -2.8236941383164513 1101 76-77 -0.1595963507295899 1101 78-79 0.11397772544949447 1101 80-81 0.100891976507274 1101 82-83 -0.04992736111285012 1101 84-85 -0.08659767286325248 1101 86-87 -0.15570457860162534 1101 88-89 -0.17538747340284644 1101 90-91 -0.2564362104306568 1101 92-93 -0.14287153655806506 1101 94-95 -0.12217370383411819 1101 96-97 -0.42250560217190625 1101 98-99 -0.16208680907669049 1101 100-101 -0.030460898964030036 1102 1 0.3791429106648323 1102 2 0.4066925738053264 1102 3 0.39766070711576873 1102 4 0.41076267987422455 1102 5 0.4785678614733939 1102 6 0.49959097584722656 1102 7 0.47592571794540817 1102 8 0.5935628117019078 1102 9 0.5934441431885134 1102 10-11 0.6789425557223794 1102 12-13 0.6325031133859937 1102 14-15 0.7665234659388886 1102 16-17 0.6944335453624717 1102 18-19 0.6113109818505862 1102 20-21 0.6905533553736092 1102 22-23 0.726954459199753 1102 24-25 0.619391578531193 1102 26-27 0.5757549896348308 1102 28-29 0.456797180496352 1102 30-31 0.27670159984132425 1102 32-33 0.6131462693040177 1102 34-35 0.4320347842775476 1102 36-37 0.46301615831639964 1102 38-39 0.7098362307189987 1102 40-41 0.48397646329222965 1102 42-43 0.3122986532302079 1102 44-45 0.30118976833610844 1102 46-47 0.4434840392434225 1102 48-49 0.43902743746494366 1102 50-51 0.3215519026323008 1102 52-53 0.1732999672103972 1102 54-55 -0.12472663410518336 1102 56-57 0.0793947746005017 1102 58-59 0.08720216273885129 1102 60-61 0.47024165460282674 1102 62-63 0.2119366641995022 1102 64-65 0.16200315137357535 1102 66-67 0.046849508901807724 1102 68-69 -6.800271124729626 1102 70-71 -7.684990525627349 1102 72-73 -1.4572551412951817 1102 74-75 0.025454106252087882 1102 76-77 0.45724578692251683 1102 78-79 0.1787387994758305 1102 80-81 -0.049165722182880955 1102 82-83 -0.10787396890041734 1102 84-85 -0.27638905641591194 1102 86-87 -0.41808599563823634 1102 88-89 -0.40142646674058113 1102 90-91 -0.4484170437705792 1102 92-93 -0.4799277579206027 1102 94-95 -0.3855181749967933 1102 96-97 -0.616769446510478 1102 98-99 -0.6468283847899841 1102 100-101 -0.5159225828293899 1103 1 0.3266715529360198 1103 2 0.2687935849246621 1103 3 0.36236753291592194 1103 4 0.2963154121265603 1103 5 0.45663087179691075 1103 6 0.39995512850111936 1103 7 0.38186397238027325 1103 8 0.43916521779780027 1103 9 0.37921087622444105 1103 10-11 0.5600716504349066 1103 12-13 0.4578633518571067 1103 14-15 0.6176954677321547 1103 16-17 0.6645103626316171 1103 18-19 0.6980338447258845 1103 20-21 0.6386440361053616 1103 22-23 0.6494437878009336 1103 24-25 0.6023206245169206 1103 26-27 0.8105751014315246 1103 28-29 0.6846738375322232 1103 30-31 0.6082865727398996 1103 32-33 0.7140023869998657 1103 34-35 0.6748680874430306 1103 36-37 0.8833033104028303 1103 38-39 0.8639767869224713 1103 40-41 0.7337165997425501 1103 42-43 0.5285121911855626 1103 44-45 0.7353451946154337 1103 46-47 0.8070461670619196 1103 48-49 0.8651668847136023 1103 50-51 0.6658132524258278 1103 52-53 0.7859468254390833 1103 54-55 0.424451654820313 1103 56-57 0.7074203233307408 1103 58-59 0.9131937875333058 1103 60-61 0.6529845000495058 1103 62-63 0.33696085575845913 1103 64-65 0.37576698371366035 1103 66-67 0.21847729723196707 1103 68-69 -6.754153230307928 1103 70-71 -7.501921256430098 1103 72-73 -0.9987264997877396 1103 74-75 0.48313079246293356 1103 76-77 0.6085103925523718 1103 78-79 0.4845357614589929 1103 80-81 0.28417116255532093 1103 82-83 0.321081513284895 1103 84-85 0.05671813262040715 1103 86-87 -0.04083885375541385 1103 88-89 0.1385944292674104 1103 90-91 -0.09971930192945777 1103 92-93 -0.1035873698933436 1103 94-95 0.09639574200420142 1103 96-97 0.15048812810148604 1103 98-99 0.03572104274646293 1103 100-101 0.096862474691914 1104 1 0.199433969469478 1104 2 0.2845742875740491 1104 3 0.15750635095036358 1104 4 0.23419136582742794 1104 5 0.4590773341760297 1104 6 0.341449760568139 1104 7 0.4615417830345123 1104 8 0.43266814833776834 1104 9 0.47438483806507037 1104 10-11 0.34984310232757565 1104 12-13 0.2847687696716079 1104 14-15 0.5333650706071538 1104 16-17 0.46447465198983906 1104 18-19 0.42822577525451067 1104 20-21 0.3319576435603864 1104 22-23 0.5043168733137549 1104 24-25 0.45694430237291783 1104 26-27 0.6292897450619961 1104 28-29 0.3753193498949088 1104 30-31 0.3827710357765568 1104 32-33 0.3158610408787226 1104 34-35 0.33085897272684406 1104 36-37 0.5394449739519089 1104 38-39 0.5862619278944763 1104 40-41 0.4944870426678776 1104 42-43 0.513312495009437 1104 44-45 0.6748332287162668 1104 46-47 0.6002951552604046 1104 48-49 0.597877265740479 1104 50-51 0.6110898115070995 1104 52-53 0.5278059327864071 1104 54-55 0.48312990760631536 1104 56-57 0.5010366209874384 1104 58-59 0.6694862293476369 1104 60-61 0.7749777206959259 1104 62-63 0.2982471190649285 1104 64-65 0.3270605025155966 1104 66-67 0.18710521501813204 1104 68-69 -6.536699795851128 1104 70-71 -4.045877088601419 1104 72-73 0.35352723849293355 1104 74-75 1.0283875122987816 1104 76-77 1.1200052513536285 1104 78-79 0.6458424308464714 1104 80-81 0.3788117321117177 1104 82-83 0.33675678451572466 1104 84-85 0.367402983372596 1104 86-87 0.336503027458523 1104 88-89 0.2993784762467442 1104 90-91 0.3287806243819169 1104 92-93 0.17272797207856527 1104 94-95 0.3660796744827479 1104 96-97 0.291944215348817 1104 98-99 0.1985269922527486 1104 100-101 0.3070281040494649 1105 1 0.185563713253714 1105 2 0.2523351402806462 1105 3 0.244007238714687 1105 4 0.16936060710497713 1105 5 0.40765493006117737 1105 6 0.3080475571012329 1105 7 0.2404082749785985 1105 8 0.42708985620386386 1105 9 0.5340856209203722 1105 10-11 0.4427600449464819 1105 12-13 0.4094974873852024 1105 14-15 0.4793291864372762 1105 16-17 0.3937052122153588 1105 18-19 0.42811633778199365 1105 20-21 0.6875324450162807 1105 22-23 0.6175329576032453 1105 24-25 0.5279605425164675 1105 26-27 0.6063765572804982 1105 28-29 0.5750363219487298 1105 30-31 0.57825409844083 1105 32-33 0.6783100250914273 1105 34-35 0.5824872325012151 1105 36-37 0.6543382818504639 1105 38-39 0.5477208803514415 1105 40-41 0.5978163947414359 1105 42-43 0.44897043120738545 1105 44-45 0.6111300533313937 1105 46-47 0.5477057325808161 1105 48-49 0.49746668653322246 1105 50-51 0.3292275630953583 1105 52-53 0.28072687553362385 1105 54-55 0.41238216664103433 1105 56-57 0.49088950306101964 1105 58-59 0.6538344065533188 1105 60-61 0.8869748979638743 1105 62-63 0.49096254578616083 1105 64-65 0.5237692648721222 1105 66-67 0.5180324334383464 1105 68-69 -3.1567100603313065 1105 70-71 -3.1443989279811717 1105 72-73 0.7909997611964918 1105 74-75 1.283677398955522 1105 76-77 1.2081343045496773 1105 78-79 0.8047982841474237 1105 80-81 0.6067264010727946 1105 82-83 0.4885859941318813 1105 84-85 0.5789965627892961 1105 86-87 0.5928989873289332 1105 88-89 0.6081091336451898 1105 90-91 0.5985734290567812 1105 92-93 0.4718064330858027 1105 94-95 0.5084083906868528 1105 96-97 0.5856653347054781 1105 98-99 0.5598317888216968 1105 100-101 0.7533552503918912 1106 1 0.10943378717816898 1106 2 0.10228616704303661 1106 3 0.08193088006912319 1106 4 0.10454646930053002 1106 5 0.22354647909306635 1106 6 0.1699724262139739 1106 7 0.21466830687593585 1106 8 0.26253680744845553 1106 9 0.30742491025242913 1106 10-11 0.349894371758694 1106 12-13 0.1469873004947928 1106 14-15 0.3109017681266195 1106 16-17 0.2947861650532886 1106 18-19 0.29798968522083413 1106 20-21 0.28356271855047055 1106 22-23 0.28180401038314074 1106 24-25 0.28512129812096987 1106 26-27 0.604800502527965 1106 28-29 0.3189530537353278 1106 30-31 0.35712515487658436 1106 32-33 0.5329310270701555 1106 34-35 0.2510851050976797 1106 36-37 0.3516688595823396 1106 38-39 0.46611543403997047 1106 40-41 0.38977697699743175 1106 42-43 0.398247371438714 1106 44-45 0.41045021644350754 1106 46-47 0.6488038984073938 1106 48-49 0.8559821652751793 1106 50-51 0.6380614059636187 1106 52-53 0.36560027628704006 1106 54-55 0.36139554250415884 1106 56-57 0.5269447735105821 1106 58-59 0.629106539334007 1106 60-61 0.8477247140286188 1106 62-63 0.4267542899493577 1106 64-65 0.4684973681988005 1106 66-67 0.6173758264687628 1106 68-69 -2.830604316219901 1106 70-71 -3.002263778601371 1106 72-73 0.8753715480418087 1106 74-75 1.2681936682763428 1106 76-77 1.2462516681900588 1106 78-79 0.8623443517411573 1106 80-81 0.5740776267746845 1106 82-83 0.5203163869383758 1106 84-85 0.41025203705283175 1106 86-87 0.316278148340448 1106 88-89 0.3499262616351828 1106 90-91 0.6332655602837356 1106 92-93 0.6739864657143215 1106 94-95 0.9386998614224886 1106 96-97 0.8452198762687537 1106 98-99 0.8704903608138821 1106 100-101 0.9577527120092135 1107 1 0.055948865694034566 1107 2 0.14287532464943453 1107 3 0.15962875636446938 1107 4 0.009781945945718462 1107 5 0.30289758402962974 1107 6 0.2043504878231488 1107 7 0.11928541505930212 1107 8 0.06777396102506117 1107 9 0.23022656602871905 1107 10-11 0.08260085522704941 1107 12-13 0.21358518072447907 1107 14-15 -0.01902900685433906 1107 16-17 0.012695421779241656 1107 18-19 0.2699282534180938 1107 20-21 0.2650834180114714 1107 22-23 0.2823902107854792 1107 24-25 0.16976873296412975 1107 26-27 0.544808925883494 1107 28-29 0.4017876612140583 1107 30-31 0.3989060624963656 1107 32-33 0.3472802067604519 1107 34-35 0.3010570164963582 1107 36-37 0.5077019267350593 1107 38-39 0.5533293318026224 1107 40-41 0.4115826483795004 1107 42-43 0.24626538361710715 1107 44-45 0.5067965376106969 1107 46-47 0.5604932229974651 1107 48-49 0.36765280175450243 1107 50-51 0.5043886875094401 1107 52-53 0.17662856741007005 1107 54-55 0.21024242641084 1107 56-57 0.5513660548022834 1107 58-59 0.5662039548602031 1107 60-61 0.43430081349903205 1107 62-63 0.3386181605263694 1107 64-65 0.07721105860243327 1107 66-67 0.33827622899680065 1107 68-69 -2.6358865373284672 1107 70-71 -2.937847763519308 1107 72-73 0.8606286603272046 1107 74-75 1.1501992105346908 1107 76-77 0.8122930168041016 1107 78-79 0.688919167714058 1107 80-81 0.5563490755337845 1107 82-83 0.515942175821742 1107 84-85 0.5115818275460491 1107 86-87 0.5977539092642061 1107 88-89 0.5651263872760453 1107 90-91 0.43381947975619894 1107 92-93 0.47267536333185234 1107 94-95 0.6770578456477203 1107 96-97 0.6871594957730132 1107 98-99 0.8801623852764422 1107 100-101 1.0745361511504683 1108 1 -0.023812739355474122 1108 2 -0.043864092326316495 1108 3 0.011068678816847921 1108 4 0.06833437023854572 1108 5 -0.05908872118364883 1108 6 -0.13338652396360118 1108 7 0.04227774220654368 1108 8 0.21430313711173454 1108 9 0.17602631272927027 1108 10-11 0.19823305109140676 1108 12-13 0.04313326013273411 1108 14-15 0.12152630889141847 1108 16-17 0.1815444972161373 1108 18-19 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-1.2075656090168465 2315 94-95 -0.014929475178558249 2315 96-97 -0.2879392572799766 2315 98-99 -2.4865874845761198 2315 100-101 -3.9809995570595262 2316 1 -0.5300623277307643 2316 2 -0.47582021822590903 2316 3 -0.8755169279878565 2316 4 -0.4854126820547364 2316 5 -0.3940188030252969 2316 6 -0.5830766268235834 2316 7 -0.7196076103029299 2316 8 -0.9487659888319229 2316 9 -5.035663568386408 2316 10-11 -1.5828378625472368 2316 12-13 -0.7499191307493973 2316 14-15 -0.6801923516024218 2316 16-17 -0.5437417329838183 2316 18-19 -0.7261452685576231 2316 20-21 -3.838716623054758 2316 22-23 -1.238362385357945 2316 24-25 -0.5415611314885496 2316 26-27 -0.6362475507303458 2316 28-29 -0.1784309636622936 2316 30-31 -0.04546227563602656 2316 32-33 -1.3630102888071818 2316 34-35 -0.5749343778267075 2316 36-37 -0.20030210120916792 2316 38-39 -5.282197438482701 2316 40-41 -4.999752205427619 2316 42-43 -3.895381909322289 2316 44-45 -2.545174545242329 2316 46-47 -2.6882272954348423 2316 48-49 -3.2202165663917306 2316 50-51 -1.5854013422251931 2316 52-53 -1.2624163933899126 2316 54-55 -0.3576033190610808 2316 56-57 -3.755008253284597 2316 58-59 -0.7985720663023379 2316 60-61 -0.7007408620029487 2316 62-63 -0.5253096703805689 2316 64-65 -0.03592387273157627 2316 66-67 -0.15662731334890267 2316 68-69 2.526899546978189 2316 70-71 3.6755429921663954 2316 72-73 2.352570013344181 2316 74-75 0.8995417191629151 2316 76-77 -1.5034970186874794 2316 78-79 -0.11319344009677934 2316 80-81 0.14793335388603168 2316 82-83 0.2413613295696564 2316 84-85 0.20165416359058597 2316 86-87 0.4695205437239487 2316 88-89 0.2727779221262061 2316 90-91 0.41251457646811573 2316 92-93 0.10548334322859176 2316 94-95 0.3134875451638486 2316 96-97 0.46393841651807577 2316 98-99 0.45438994685418876 2316 100-101 0.4013361087409564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 57.0 4 109.0 5 389.0 6 888.0 7 2014.0 8 4088.0 9 6948.0 10 10505.0 11 14990.0 12 19645.0 13 24016.0 14 29165.0 15 35844.0 16 42478.0 17 47684.0 18 44027.0 19 39301.0 20 38728.0 21 39388.0 22 41799.0 23 46529.0 24 54311.0 25 66730.0 26 83019.0 27 102373.0 28 124350.0 29 146972.0 30 170929.0 31 197274.0 32 227794.0 33 262172.0 34 299943.0 35 330563.0 36 325124.0 37 250455.0 38 92404.0 39 4292.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.86083485943681 20.26995993029253 15.463547537412909 22.405657672857753 2 16.206100504010735 21.908242960259606 37.590312382523784 24.295344153205875 3 19.19898019574342 25.61455662745725 30.480781684672507 24.70568149212682 4 13.270067220245158 17.777095496166044 36.36805991673063 32.58477736685817 5 13.693274299594057 38.21102237872009 33.14961979136165 14.9460835303242 6 31.41681284117067 36.54929636381045 16.935730006472888 15.098160788545991 7 27.943080717142806 34.041911723923384 20.138493177128794 17.876514381805016 8 27.136401959900315 33.7645907253323 19.467624245229846 19.63138306953754 9 26.794042215382667 16.654009054608068 20.26785800049391 36.28409072951535 10-11 25.256326426893587 26.86224410742065 26.336575827254304 21.544853638431455 12-13 26.31605855904009 24.34954282316495 27.41613983423331 21.91825878356165 14-15 23.25170800298825 25.50862065492995 26.29438414132898 24.945287200752826 16-17 22.08412727898358 28.970961226428937 26.705932859736585 22.2389786348509 18-19 21.69865107139028 26.838703040496366 30.0046602394198 21.457985648693555 20-21 22.674554977261227 25.919148689604054 29.785607628899264 21.620688704235448 22-23 22.330596166520465 25.85686872289339 29.802361290526484 22.01017382005966 24-25 22.337780449524896 26.17604816581634 29.876083955521608 21.61008742913716 26-27 22.19996523303011 26.429280357808516 29.77394342651728 21.596810982644094 28-29 21.736044642024396 26.43566255473274 30.23729321938367 21.59099958385919 30-31 21.78679645933243 26.4077475264935 29.987116169207646 21.818339844966427 32-33 21.733645277541985 26.526448767408784 29.59512581235041 22.144780142698828 34-35 22.394158697618124 26.3376854204951 29.46044520285304 21.80771067903374 36-37 22.519014662949406 26.08176448504917 29.55085403744767 21.848366814553753 38-39 22.130195812782862 26.29300284469233 29.725708881727144 21.851092460797663 40-41 22.023857332333954 26.25567151233196 30.03272583945825 21.68774531587583 42-43 22.075347679379984 26.584011423579383 29.21292945995553 22.127711437085107 44-45 22.047854801598398 26.798803637712922 28.95836812437851 22.194973436310175 46-47 22.18165782562671 26.94453131892728 28.44973833509141 22.424072520354603 48-49 22.52520007572465 26.900608913647382 28.281281421117903 22.292909589510057 50-51 22.47462621725239 27.125112932152025 28.08057281699262 22.319688033602965 52-53 22.656084321436413 27.10716779335841 28.005064222049427 22.231683663155756 54-55 22.036547591681703 26.91238092256078 28.295095196073888 22.755976289683634 56-57 22.32002954365864 27.52928446975843 27.76271718595102 22.38796880063191 58-59 22.476050361764628 26.82094498738539 27.901489316433683 22.801515334416305 60-61 22.33010385694835 26.7828269970735 28.477368537915993 22.40970060806216 62-63 22.472802619946883 26.931642229384174 28.19753777297698 22.398017377691968 64-65 22.282336124113353 26.79756172270925 28.23652523020178 22.683576922975618 66-67 21.8643787165521 27.11868588630006 28.373963213766928 22.642972183380913 68-69 21.947004086861334 26.67562719259502 28.864577965159455 22.512790755384195 70-71 21.5066580230573 26.680880578907146 29.18337431856932 22.62908707946623 72-73 22.00727540736606 26.849362993692523 28.140153049443693 23.003208549497725 74-75 22.060086422363415 27.298891911280975 27.693444288950054 22.947577377405555 76-77 21.578443662217804 27.145120289984437 28.375287675273313 22.901148372524442 78-79 22.066854681998578 27.11435512709009 27.904301528620117 22.914488662291213 80-81 21.807146651892264 27.759622224009117 27.67158934972647 22.761641774372148 82-83 22.139554686016055 27.456417241794952 27.40200560786874 23.00202246432025 84-85 21.981486407619734 27.169112799861306 27.384416313985867 23.46498447853309 86-87 22.213654560234925 27.125016442009553 27.52980361152516 23.131525386230358 88-89 22.02242835610031 27.014219477993745 27.46437926333743 23.49897290256851 90-91 21.823432113134135 27.193867211538997 27.76221206267519 23.220488612651675 92-93 21.90316594214597 27.221955564452184 27.612217560101026 23.26266093330082 94-95 21.70785172634283 26.928929413785813 27.645083685965677 23.718135173905683 96-97 21.771439553269346 26.74750201868817 27.537091942140755 23.94396648590173 98-99 21.669780873373448 26.840458976066188 27.234121148348162 24.255639002212202 100-101 21.698264938329682 26.612088256375976 27.64424991408208 24.045396891212263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5008.0 1 3439.0 2 2029.0 3 2370.5 4 2229.0 5 2210.5 6 3640.5 7 6549.0 8 9401.5 9 10688.0 10 9526.0 11 7482.5 12 6644.5 13 6321.0 14 6142.5 15 6158.0 16 6254.0 17 6379.5 18 6530.5 19 6809.5 20 7251.0 21 8112.5 22 9267.0 23 10485.5 24 12106.0 25 14497.0 26 17487.0 27 20829.0 28 24618.0 29 29067.5 30 34194.0 31 40144.5 32 46844.5 33 52083.5 34 56546.5 35 61739.0 36 68213.5 37 74556.0 38 77930.5 39 78672.5 40 79973.0 41 86387.5 42 93911.0 43 99114.5 44 102601.0 45 106168.0 46 110017.5 47 117724.0 48 136924.0 49 140675.0 50 127596.5 51 120513.0 52 115780.0 53 114096.5 54 111839.5 55 103674.5 56 93315.0 57 83853.5 58 75536.5 59 67738.0 60 58644.5 61 48787.0 62 40064.5 63 31914.0 64 24588.0 65 19215.0 66 14853.5 67 11804.0 68 8468.5 69 5502.0 70 4110.5 71 3289.0 72 2724.0 73 2286.5 74 1809.5 75 1368.5 76 932.0 77 565.5 78 360.5 79 226.0 80 143.5 81 96.5 82 64.0 83 41.5 84 26.0 85 11.5 86 7.5 87 6.0 88 2.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021070198775635537 2 0.018281495996507302 3 6.506973151299211E-4 4 0.08775118078323506 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011371710221556239 12-13 0.0 14-15 0.0 16-17 5.732333490430256E-4 18-19 1.549279321737907E-4 20-21 0.0 22-23 1.2394234573903258E-4 24-25 0.0037802415450404934 26-27 0.0032844721620843634 28-29 0.001053509938781777 30-31 1.8591351860854885E-4 32-33 6.352045219125419E-4 34-35 0.0025253252944327886 36-37 0.08947088083036414 38-39 0.1001609081503557 40-41 0.08173997701489198 42-43 0.08516388431593276 44-45 0.08361460499419486 46-47 0.08265405181471734 48-49 0.07639496335489619 50-51 0.0766118624599395 52-53 0.05995710975125701 54-55 0.05027411399039509 56-57 0.027747592652325917 58-59 0.021457518606070015 60-61 0.012905496750076769 62-63 0.0020450487046940373 64-65 0.005964725388690943 66-67 0.1595912629322218 68-69 0.7320654651075958 70-71 0.8923074253549477 72-73 0.3618961567647577 74-75 0.05431773302013102 76-77 0.19968661177879884 78-79 0.013711121997380478 80-81 0.0032070081959974677 82-83 0.001735192840346456 84-85 7.746396608689535E-5 86-87 0.001719700047129077 88-89 2.6337748469544423E-4 90-91 0.0039971406500838 92-93 0.0035943280264319447 94-95 0.07786677871054722 96-97 0.03724467489457929 98-99 0.013277323787293864 100-101 0.012084378709555676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3227307.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.07384164709973 #Duplication Level Percentage of deduplicated Percentage of total 1 64.31402667072838 21.271119337950186 2 14.102922361791107 9.328756419104415 3 6.720066331853181 6.667752291531552 4 3.942563820888228 5.215829259825669 5 2.471657303490678 4.08736011307741 6 1.6719792932869504 3.3179267030041393 7 1.2196236732154986 2.8236348165888456 8 0.9169727063936697 2.4262248068781362 9 0.6951838650188845 2.0693160960528005 >10 3.5361667315574463 20.441884650898974 >50 0.2334120205444405 5.350247820045495 >100 0.15894511627954222 9.953647913541136 >500 0.011976820459358778 2.700647432158993 >1k 0.004120026237969909 2.509210817908759 >5k 9.581456367371884E-5 0.22005228425453607 >10k+ 2.874436910211565E-4 1.616389237179021 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21134 0.6548493837121786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17419 0.5397379301070521 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12454 0.3858944934584779 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6944 0.21516391220296055 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4796 0.14860687254110005 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4293 0.13302112256441673 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4289 0.1328971802186777 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4109 0.12731977466042121 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.0985586434758144E-5 0.0 0.0 0.0 0.0 9 3.0985586434758144E-5 0.0 0.0 0.0 0.0 10-11 3.0985586434758144E-5 0.0 0.0 0.0 0.0 12-13 7.746396608689535E-5 0.0 0.0 1.5492793217379072E-5 0.0 14-15 9.295675930427443E-5 0.0 0.0 3.0985586434758144E-5 0.0 16-17 1.084495525216535E-4 0.0 0.0 1.3943513895641165E-4 0.0 18-19 1.2394234573903258E-4 0.0 0.0 2.6337748469544423E-4 0.0 20-21 1.2394234573903258E-4 0.0 0.0 5.112621761735093E-4 0.0 22-23 1.2394234573903258E-4 0.0 0.0 0.0013788585963467373 0.0 24-25 1.2394234573903258E-4 0.0 0.0 0.0025098325012154095 0.0 26-27 1.2394234573903258E-4 0.0 0.0 0.0039661550636490425 0.0 28-29 1.2394234573903258E-4 0.0 0.0 0.006661901083473001 0.0 30-31 1.2394234573903258E-4 0.0 0.0 0.013881542722771648 0.0 32-33 1.2394234573903258E-4 0.0 0.0 0.02588845746624043 0.0 34-35 1.2394234573903258E-4 0.0 0.0 0.042667152520661965 0.0 36-37 1.2394234573903258E-4 0.0 0.0 0.07046122355264002 0.0 38-39 1.2394234573903258E-4 0.0 0.0 0.10995235346373927 0.0 40-41 1.2394234573903258E-4 0.0 0.0 0.15498990334666024 0.0 42-43 1.3943513895641165E-4 0.0 0.0 0.20027533792105928 0.0 44-45 1.5492793217379073E-4 0.0 0.0 0.2562198142290151 0.0 46-47 1.5492793217379073E-4 0.0 0.0 0.3245895106973089 0.0 48-49 1.5492793217379073E-4 0.0 0.0 0.39729718926646895 0.0 50-51 1.5492793217379073E-4 0.0 0.0 0.4775343653392751 0.0 52-53 1.5492793217379073E-4 0.0 0.0 0.5530772250672155 0.0 54-55 1.5492793217379073E-4 0.0 0.0 0.6400537661895815 0.0 56-57 2.16899105043307E-4 0.0 0.0 0.736403447208462 0.0 58-59 2.16899105043307E-4 0.0 0.0 0.837354487812904 1.5492793217379072E-5 60-61 2.16899105043307E-4 0.0 0.0 0.9417914068912564 3.0985586434758144E-5 62-63 2.16899105043307E-4 0.0 0.0 1.0461353692103046 3.0985586434758144E-5 64-65 2.16899105043307E-4 0.0 0.0 1.1519666396782209 3.0985586434758144E-5 66-67 2.16899105043307E-4 0.0 0.0 1.26865835819152 3.0985586434758144E-5 68-69 2.16899105043307E-4 0.0 0.0 1.3997893599834166 3.0985586434758144E-5 70-71 2.16899105043307E-4 0.0 0.0 1.5722551340792803 3.0985586434758144E-5 72-73 2.3239189826068608E-4 0.0 0.0 1.7637460582460858 3.0985586434758144E-5 74-75 2.4788469147806516E-4 0.0 0.0 1.9678326232986203 3.0985586434758144E-5 76-77 2.4788469147806516E-4 0.0 0.0 2.2007202909422623 3.0985586434758144E-5 78-79 2.4788469147806516E-4 0.0 0.0 2.462641453075273 3.0985586434758144E-5 80-81 3.0985586434758146E-4 0.0 0.0 2.7548355331550423 3.0985586434758144E-5 82-83 3.0985586434758146E-4 0.0 0.0 3.052421105274459 3.0985586434758144E-5 84-85 3.0985586434758146E-4 0.0 0.0 3.3653600354722992 3.0985586434758144E-5 86-87 3.0985586434758146E-4 0.0 0.0 3.696394548148038 3.0985586434758144E-5 88-89 3.0985586434758146E-4 0.0 0.0 4.042782418902199 3.0985586434758144E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19525 0.0 25.476301 1 GTATCAA 26795 0.0 25.171349 1 GTACATG 18450 0.0 23.07609 1 TACATGG 18715 0.0 22.261251 2 GAGTACT 15880 0.0 21.94174 12-13 ACATGGG 18660 0.0 21.357243 3 AGTACTT 16505 0.0 20.823645 12-13 TATCAAC 32575 0.0 20.611847 2 ATCAACG 32660 0.0 20.525963 3 GTACTTT 17060 0.0 20.451872 14-15 TCAACGC 33110 0.0 20.221785 4 CAACGCA 33375 0.0 20.058952 5 AACGCAG 33740 0.0 19.856007 6 TACTTTT 18290 0.0 18.66178 14-15 ACTTTTT 19525 0.0 18.476704 16-17 CCCTATA 440 0.0 18.33332 2 CATGGGG 7720 0.0 18.19391 4 ACGCAGA 37425 0.0 17.837568 7 CATGGGA 10600 0.0 17.771997 4 CGCAGAG 37490 0.0 17.768702 8 >>END_MODULE