FastQCFastQC Report
Fri 27 May 2016
SRR939403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332135
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87270.3742064674643621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73490.315118978961338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.20166071003608282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24050.10312439031188161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56500.049.4154321
GTATCAA97450.039.956941
TATCAAC124550.031.6821352
ATCAACG126050.031.3735733
TCAACGC128450.030.9521484
CAACGCA129750.030.3726565
AACGCAG133750.029.19466
CGCAGAG150700.025.3598568
ACGCAGA150200.025.3321387
GTACATG99650.024.4655281
TACATGG101950.023.9877552
GCAGAGT154500.022.9566049
ACATGGG105650.022.11453
GAGTACT90250.021.5084412-13
GTGGTAT31400.021.1176221
AGAGTAC144100.020.0648610-11
CAGAGTA150900.020.0360410-11
AGTACTT95100.019.8326612-13
GTACTTT99900.019.8231914-15
CATGGGA64100.018.9583074
Produced by FastQC (version 0.11.5)