FastQCFastQC Report
Fri 27 May 2016
SRR939403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332135
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124480.5337598380882753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119010.5103049351774233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76500.32802560743696224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38600.1655135744714607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.12915204308498437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.11324387310340095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.056.55291
GTATCAA103350.036.5957451
ATCAACG136400.027.3687153
TATCAAC137950.027.1007752
TCAACGC139250.026.8085674
GTGGTAT28800.026.4954661
CAACGCA141000.026.408645
AACGCAG145300.025.6597086
TGGTATC28750.025.5458432
GTACATG107250.023.7309511
TACATGG111050.021.8462922
ACGCAGA169750.021.8242637
CGCAGAG170600.021.6877548
ACATGGG111050.021.2448353
GAGTACT107650.021.10168812-13
GGACCGT2257.362905E-821.0552356
GCAGAGT181600.019.7740659
CATGGGA70000.019.6941644
CAGAGTA176250.019.46041110-11
GTACTTT117650.019.4288914-15
Produced by FastQC (version 0.11.5)