##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939403_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332135 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.953452523117228 31.0 31.0 31.0 30.0 34.0 2 31.217247286284884 31.0 31.0 34.0 30.0 34.0 3 31.20038505489605 31.0 31.0 34.0 28.0 34.0 4 35.14279747956272 35.0 35.0 37.0 33.0 37.0 5 34.76469715518184 35.0 35.0 37.0 32.0 37.0 6 34.72424923943082 35.0 35.0 37.0 31.0 37.0 7 34.59668586938578 35.0 35.0 37.0 30.0 37.0 8 34.612465401874246 35.0 35.0 37.0 30.0 37.0 9 35.956666316486825 37.0 35.0 39.0 30.0 39.0 10-11 35.826504040289265 37.0 35.0 39.0 30.0 39.0 12-13 35.78092220218812 37.0 35.0 39.0 30.0 39.0 14-15 36.627770476408955 38.0 35.5 40.0 30.0 41.0 16-17 36.631934900852656 38.0 35.0 40.0 30.5 41.0 18-19 36.597927649985955 38.0 35.0 40.0 30.0 41.0 20-21 36.503695969572945 38.0 35.0 40.0 30.0 41.0 22-23 36.34839728403373 38.0 34.5 40.0 30.0 41.0 24-25 36.13081639785004 38.0 34.0 40.0 29.0 41.0 26-27 35.751724064001436 38.0 34.0 40.0 27.5 41.0 28-29 35.46291488271476 38.0 34.0 40.0 27.0 41.0 30-31 35.233128013601274 38.0 34.0 40.0 27.0 41.0 32-33 35.00980496412086 37.5 33.0 40.0 27.0 41.0 34-35 34.97058017653352 38.0 33.0 40.0 26.0 41.0 36-37 34.96201742180449 38.0 33.0 40.0 25.5 41.0 38-39 34.92418899420488 38.0 33.0 40.0 26.0 41.0 40-41 34.698089090039815 38.0 33.0 40.0 25.0 41.0 42-43 34.399177363231544 37.5 33.0 40.0 24.5 41.0 44-45 34.20491545300765 37.0 33.0 40.0 24.0 41.0 46-47 33.876669232270004 37.0 32.5 40.0 23.5 41.0 48-49 33.555102513362215 37.0 32.0 40.0 22.5 41.0 50-51 33.28775649780137 37.0 32.0 40.0 21.5 41.0 52-53 32.923555025759654 36.5 31.0 39.5 20.0 41.0 54-55 32.69308294759952 36.0 31.0 39.0 19.5 40.5 56-57 32.288736715498885 36.0 31.0 39.0 18.0 40.0 58-59 31.850160904064303 35.0 30.0 39.0 15.0 40.0 60-61 31.54994136274272 35.0 30.0 38.5 13.5 40.0 62-63 31.15846145270321 35.0 29.0 38.0 12.0 40.0 64-65 30.713303475141878 34.5 28.5 38.0 8.0 40.0 66-67 28.950047917466186 33.5 25.0 37.0 5.0 39.5 68-69 25.179737879668203 29.5 15.5 35.0 2.0 38.0 70-71 24.555552101400647 28.5 13.5 34.5 2.0 38.0 72-73 26.45224225870286 31.0 21.0 35.0 2.0 38.0 74-75 27.100584014218732 31.5 23.5 35.0 2.0 37.5 76-77 27.20710421995296 31.5 25.0 34.5 2.0 36.5 78-79 28.06524429331921 32.5 26.0 35.0 2.0 37.0 80-81 27.930781665726897 33.0 26.0 35.0 2.0 36.5 82-83 27.52014999131697 32.5 26.0 35.0 2.0 36.0 84-85 27.173050230797102 32.0 25.5 35.0 2.0 36.0 86-87 26.726149429599914 32.0 25.0 34.5 2.0 35.0 88-89 26.23464615041582 32.0 24.0 34.0 2.0 35.0 90-91 25.76160985534714 32.0 23.5 34.0 2.0 35.0 92-93 25.17442986791073 31.0 20.5 34.0 2.0 35.0 94-95 23.75309276692816 31.0 10.0 34.0 2.0 35.0 96-97 22.929384662551698 30.0 4.0 34.0 2.0 35.0 98-99 22.589559137871518 30.0 2.0 34.0 2.0 35.0 100-101 21.324872702480775 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4262102574984503 1101 2 0.28426385470987015 1101 3 0.43578267930796954 1101 4 0.3630120636823122 1101 5 0.4372836566805347 1101 6 0.6284412961487149 1101 7 0.5046776572688216 1101 8 0.5438916441090669 1101 9 0.6474445575758665 1101 10-11 0.7450064518222632 1101 12-13 0.7023962472298066 1101 14-15 0.7068234888957008 1101 16-17 0.6728052070345569 1101 18-19 0.8521599746483233 1101 20-21 0.9202371396495792 1101 22-23 0.8875193670118335 1101 24-25 0.9467480087324489 1101 26-27 0.9163234743293245 1101 28-29 0.8085157162447203 1101 30-31 0.9260602252486763 1101 32-33 0.9099889086537658 1101 34-35 0.9512525164956216 1101 36-37 1.0746991254007767 1101 38-39 0.8482407735894171 1101 40-41 1.0379720941569488 1101 42-43 0.897711278056029 1101 44-45 0.6542291782005734 1101 46-47 0.5488157354124183 1101 48-49 0.4799248753580727 1101 50-51 0.4444568823370858 1101 52-53 0.6208717350620958 1101 54-55 0.5084293275582965 1101 56-57 0.3674509632866432 1101 58-59 0.32373958154799354 1101 60-61 0.08822992173928768 1101 62-63 -0.02413660491010461 1101 64-65 -0.0029962198742623514 1101 66-67 -3.0908223744698624 1101 68-69 -8.201338172048604 1101 70-71 -11.283740651019844 1101 72-73 -11.207038871104864 1101 74-75 -9.602662085870541 1101 76-77 -4.562492636059808 1101 78-79 -1.618559101047822 1101 80-81 -0.9977400654321329 1101 82-83 -0.70876766765047 1101 84-85 -0.8247375430576867 1101 86-87 -0.879722642572272 1101 88-89 -0.7278892324789688 1101 90-91 -0.7236402877402739 1101 92-93 -1.296584327474367 1101 94-95 -4.987881193584851 1101 96-97 -1.7894020061188165 1101 98-99 -0.9279933431748617 1101 100-101 -0.8188823302872663 1102 1 0.4172112917962245 1102 2 0.268559068542789 1102 3 0.4444456420001366 1102 4 0.15640129345771214 1102 5 0.5415638475915827 1102 6 0.617247357237737 1102 7 0.5301930741110894 1102 8 0.5431144831126886 1102 9 0.6986813197555648 1102 10-11 0.683423905028981 1102 12-13 0.6887577289620097 1102 14-15 0.6353367946970536 1102 16-17 0.6677363401095491 1102 18-19 0.647640323401383 1102 20-21 0.8146983066562186 1102 22-23 0.7644735996776291 1102 24-25 0.7723352677831059 1102 26-27 0.911135212691697 1102 28-29 0.7000738254596612 1102 30-31 0.6474857296571059 1102 32-33 0.5051585436208654 1102 34-35 0.7669289874946159 1102 36-37 0.9519337713365772 1102 38-39 0.8790967761722825 1102 40-41 0.6295175792979322 1102 42-43 0.7617171404459597 1102 44-45 0.588863037610011 1102 46-47 0.7251923961559044 1102 48-49 0.6647644578818586 1102 50-51 0.5728088683558568 1102 52-53 0.6853042144767727 1102 54-55 0.6375384841743141 1102 56-57 0.40760147456164475 1102 58-59 0.37793985193868096 1102 60-61 0.3213188655972097 1102 62-63 0.16012852747973838 1102 64-65 0.21101455796311797 1102 66-67 -2.7866591410857815 1102 68-69 -7.8087276915478725 1102 70-71 -11.146892129227709 1102 72-73 -9.448383575963053 1102 74-75 -7.894008871920967 1102 76-77 -4.320010121497624 1102 78-79 -1.535811171491801 1102 80-81 -0.9127618371675155 1102 82-83 -0.5887726269125011 1102 84-85 -0.6666577112618342 1102 86-87 -0.7669578279750056 1102 88-89 -0.6183307546739272 1102 90-91 -0.7844678546649781 1102 92-93 -0.8704950629485353 1102 94-95 -4.315586476499657 1102 96-97 -1.431522105698388 1102 98-99 -0.6688723812697184 1102 100-101 -0.480898353824454 1103 1 0.36669025219428164 1103 2 0.22776575845006874 1103 3 0.2930336849806068 1103 4 0.16025286317711362 1103 5 0.4009862384413694 1103 6 0.34699534487099015 1103 7 0.4724822747219264 1103 8 0.37163956782033125 1103 9 0.5567122887718767 1103 10-11 0.6194309277366585 1103 12-13 0.5070848935906369 1103 14-15 0.6666150611362056 1103 16-17 0.6251087163728286 1103 18-19 0.5146175440128431 1103 20-21 0.742383893400266 1103 22-23 0.757181775126341 1103 24-25 0.7983896925452783 1103 26-27 0.8311453662003672 1103 28-29 0.8138000490552386 1103 30-31 0.700946071486463 1103 32-33 0.6986664097379744 1103 34-35 0.9692470188547802 1103 36-37 0.8524654452313101 1103 38-39 0.792030439372418 1103 40-41 0.8205872995141661 1103 42-43 0.8042724428428656 1103 44-45 0.6565922779222078 1103 46-47 0.7213247323918708 1103 48-49 0.6108612242419653 1103 50-51 0.500286251130099 1103 52-53 0.6946756340611486 1103 54-55 0.5123225387653108 1103 56-57 0.6729173008339728 1103 58-59 0.5702789644600905 1103 60-61 0.4875937746964176 1103 62-63 0.47990537845784687 1103 64-65 0.27705697942010943 1103 66-67 -3.159335283274018 1103 68-69 -7.7887189233493785 1103 70-71 -11.709278757887983 1103 72-73 -9.444301800288773 1103 74-75 -7.732151065046246 1103 76-77 -4.0402954220528855 1103 78-79 -1.0834312086181157 1103 80-81 -0.33811623210478103 1103 82-83 -0.006078573478646376 1103 84-85 -0.14269816068905072 1103 86-87 -0.03861674505359858 1103 88-89 0.07387451243832288 1103 90-91 0.152214303028952 1103 92-93 0.21601633489461847 1103 94-95 1.121031305015741 1103 96-97 1.2404020167891083 1103 98-99 0.6418991387089044 1103 100-101 0.25657844223018245 1104 1 0.42045834533461957 1104 2 0.24084886889689372 1104 3 0.3904613124192444 1104 4 0.13554359896481571 1104 5 0.3077557606775727 1104 6 0.44525715785609776 1104 7 0.4456916438219878 1104 8 0.4223921375706965 1104 9 0.6348323721576605 1104 10-11 0.7665491362535448 1104 12-13 0.6834635122187009 1104 14-15 0.7427979985182063 1104 16-17 0.753837968260811 1104 18-19 0.7531765827164278 1104 20-21 0.8431539990746941 1104 22-23 0.6878534174066075 1104 24-25 0.7894291631242609 1104 26-27 0.969833220196584 1104 28-29 1.0196980194768912 1104 30-31 1.0919144150463538 1104 32-33 0.7783262128111872 1104 34-35 1.0138161267842989 1104 36-37 1.0669560184429727 1104 38-39 1.0107583146084167 1104 40-41 1.1394362873471877 1104 42-43 1.3453638656454743 1104 44-45 1.122266935725854 1104 46-47 1.1446321060021063 1104 48-49 1.177395306211757 1104 50-51 1.2604554482142234 1104 52-53 1.3581284738975228 1104 54-55 1.2404796606449011 1104 56-57 1.0817829396004868 1104 58-59 1.1892356261445904 1104 60-61 1.1639174911323273 1104 62-63 1.1217111853070172 1104 64-65 0.9580916317171493 1104 66-67 -2.246940334931246 1104 68-69 -6.833096283324206 1104 70-71 -8.813919877827146 1104 72-73 -8.0774011027851 1104 74-75 -6.7121691254091544 1104 76-77 -3.186757262571035 1104 78-79 -0.22843527301920474 1104 80-81 0.5778752195419585 1104 82-83 0.8684822089841227 1104 84-85 0.8221906582746605 1104 86-87 1.1508777614494434 1104 88-89 1.0156331197909552 1104 90-91 0.9574745885859066 1104 92-93 1.03337476918243 1104 94-95 1.9273060519739964 1104 96-97 1.9491057350605594 1104 98-99 1.56506638881379 1104 100-101 1.5269176558341222 1105 1 0.4928631072393834 1105 2 0.34813160699212986 1105 3 0.5085327409906739 1105 4 0.3638144322981489 1105 5 0.4628301654394775 1105 6 0.5727482292846631 1105 7 0.6488493819172305 1105 8 0.548368328046891 1105 9 0.8630793959671834 1105 10-11 0.8963348505392617 1105 12-13 0.8346242265044239 1105 14-15 0.9474319270896316 1105 16-17 1.0476579087370013 1105 18-19 0.9585902731926197 1105 20-21 1.0602001300270771 1105 22-23 1.083572167406608 1105 24-25 0.9352044607433143 1105 26-27 1.0246427440061083 1105 28-29 1.0944658766197435 1105 30-31 1.1235795936177766 1105 32-33 1.1363693675730886 1105 34-35 1.3249441029747757 1105 36-37 1.410423280347736 1105 38-39 1.276777659846509 1105 40-41 1.4355136682995706 1105 42-43 1.4863192227883317 1105 44-45 1.4282178285830085 1105 46-47 1.4690249631449603 1105 48-49 1.162268818116516 1105 50-51 1.1892084244046899 1105 52-53 1.4386254977070507 1105 54-55 1.5281939463591883 1105 56-57 1.3704734157909684 1105 58-59 1.4091656856683947 1105 60-61 1.3240261220847067 1105 62-63 1.4380400543546408 1105 64-65 1.4608981793361977 1105 66-67 2.0953498436401823 1105 68-69 -4.851018902371823 1105 70-71 -5.385995770684289 1105 72-73 -1.4844427694517641 1105 74-75 1.3185942674479882 1105 76-77 1.5488870826670649 1105 78-79 1.4235706793617453 1105 80-81 1.3498395052562486 1105 82-83 1.4616369708888826 1105 84-85 1.219904943988947 1105 86-87 1.4241426424018222 1105 88-89 1.3940096078861934 1105 90-91 1.44516060049067 1105 92-93 1.5356500668014768 1105 94-95 2.3592152781644735 1105 96-97 2.2439688302986553 1105 98-99 2.0531095435756974 1105 100-101 2.0318060486912692 1106 1 0.27648215485843153 1106 2 0.20756017842070307 1106 3 0.1805327409906745 1106 4 0.05410014658386331 1106 5 0.2719730225823369 1106 6 0.3483672769037156 1106 7 0.27703985810770604 1106 8 0.2637968994754587 1106 9 0.5175555864433719 1106 10-11 0.37481104101545526 1106 12-13 0.3277670836472737 1106 14-15 0.43238430804201045 1106 16-17 0.25280076587985434 1106 18-19 0.37535217795451814 1106 20-21 0.4813429871699313 1106 22-23 0.46585788169232245 1106 24-25 0.5161568416956968 1106 26-27 0.7212141725775396 1106 28-29 0.7790373051911743 1106 30-31 0.6820557840939756 1106 32-33 0.6784646056683314 1106 34-35 0.9257060077366788 1106 36-37 0.7132804232048713 1106 38-39 0.6642062312750809 1106 40-41 0.8223708111567092 1106 42-43 0.8695573180264233 1106 44-45 0.741551161916334 1106 46-47 0.5886440107640141 1106 48-49 0.5677926276403298 1106 50-51 0.5191131863094611 1106 52-53 0.6818635929451489 1106 54-55 0.6824796606449013 1106 56-57 0.8647591300766777 1106 58-59 0.9108799713826805 1106 60-61 0.7623118363704258 1106 62-63 0.7519448162593996 1106 64-65 0.8239457983838179 1106 66-67 1.4873498436401853 1106 68-69 -5.12225699760992 1106 70-71 -6.262376723065245 1106 72-73 -2.3202522932612872 1106 74-75 0.5039276007813207 1106 76-77 0.9795537493337321 1106 78-79 0.7591897269807966 1106 80-81 0.8479347433514839 1106 82-83 0.7976369708888811 1106 84-85 0.8084763725603743 1106 86-87 0.9963331185923003 1106 88-89 0.8185810364576227 1106 90-91 0.7375415528716189 1106 92-93 0.7510786382300516 1106 94-95 1.7100724210216143 1106 96-97 2.0232069255367513 1106 98-99 1.723585734051884 1106 100-101 1.4024727153579342 1107 1 -0.06609075948671617 1107 2 -0.1232614544446129 1107 3 -0.023034155970918846 1107 4 -0.02867676449257317 1107 5 -0.0018626508446004664 1107 6 0.13838510618831634 1107 7 0.051632533243278544 1107 8 -0.04468404547662175 1107 9 0.15835344692921893 1107 10-11 0.047132859602477595 1107 12-13 0.14871381865953737 1107 14-15 0.13234523219326633 1107 16-17 0.057656348674598235 1107 18-19 0.2065644950186325 1107 20-21 0.07525361788087537 1107 22-23 0.07347640929056354 1107 24-25 0.2444836376246684 1107 26-27 0.31444914769083 1107 28-29 0.3260053610562679 1107 30-31 0.2586214181479036 1107 32-33 0.2125995882104874 1107 34-35 0.2309938763942867 1107 36-37 0.25671776069837193 1107 38-39 0.20735896881316052 1107 40-41 0.04548083270042724 1107 42-43 0.29093173300302766 1107 44-45 0.28663384522366897 1107 46-47 0.016458586204173287 1107 48-49 0.038328917663356776 1107 50-51 0.19305761837245683 1107 52-53 0.26002271931020005 1107 54-55 0.1802222503484927 1107 56-57 0.14096780699517808 1107 58-59 0.24945734304897726 1107 60-61 0.17503206517957537 1107 62-63 0.18667693125828677 1107 64-65 0.22223143838084525 1107 66-67 1.1597063550434932 1107 68-69 -5.551847444084924 1107 70-71 -6.100789991024534 1107 72-73 -2.285450049742977 1107 74-75 0.2840111012641984 1107 76-77 0.31314204143684066 1107 78-79 0.26145798342533766 1107 80-81 0.21362154225201024 1107 82-83 0.3800271350668787 1107 84-85 0.43170146727845093 1107 86-87 0.5969331483077731 1107 88-89 0.408091251151923 1107 90-91 0.5242279060400286 1107 92-93 0.6278458917773335 1107 94-95 1.3676798053169854 1107 96-97 1.5392980034655785 1107 98-99 1.1594690265264411 1107 100-101 1.0218918521234066 1108 1 0.0933987656341948 1108 2 -0.035100228465058336 1108 3 -0.05472368646293546 1108 4 -0.001830995826146875 1108 5 0.05742216186246196 1108 6 0.15017590645881995 1108 7 0.16377269960111107 1108 8 -0.01569471688939217 1108 9 0.132979239472661 1108 10-11 0.20463599071364058 1108 12-13 0.281925813806005 1108 14-15 0.22985401517369297 1108 16-17 0.0990053265773767 1108 18-19 0.2475927323922562 1108 20-21 0.3222848606329265 1108 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1.5277824316118078 2312 94-95 2.095078070739131 2312 96-97 2.3438671353834017 2312 98-99 2.0742717711786014 2312 100-101 1.924687404623473 2313 1 -0.19217392979765435 2313 2 -0.22291601205548872 2313 3 -0.09161540715747307 2313 4 -0.0540480015642828 2313 5 -0.10341322080390825 2313 6 0.011436059972496082 2313 7 -3.267362258294412 2313 8 -1.0567745290959678 2313 9 -0.7892169003291158 2313 10-11 -0.19542176321734672 2313 12-13 -1.3889736570934659 2313 14-15 -0.43705484539713524 2313 16-17 -0.10109341401432204 2313 18-19 0.24427281287515967 2313 20-21 0.004856214683158555 2313 22-23 -1.300163282328839 2313 24-25 -2.4940653805265285 2313 26-27 -1.9154842401208754 2313 28-29 -0.41335422920035825 2313 30-31 -0.23275903072084247 2313 32-33 -2.549133277929556 2313 34-35 -2.7559342039035286 2313 36-37 -1.5055555556310978 2313 38-39 -0.8770847740159198 2313 40-41 -0.8693434745575743 2313 42-43 -0.969839507370402 2313 44-45 -0.4259514835863101 2313 46-47 -0.2182977881777859 2313 48-49 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2314 8 -0.20365880209300258 2314 9 0.08343943553197164 2314 10-11 0.16364359718331656 2314 12-13 -0.10317144962953506 2314 14-15 -2.8270521763440257 2314 16-17 -2.954751586811959 2314 18-19 -0.5460597197422459 2314 20-21 -0.03995247700980542 2314 22-23 -0.037237215100105914 2314 24-25 -2.1891103619216636 2314 26-27 -1.3211975838164065 2314 28-29 -0.44077560988583286 2314 30-31 0.04364967981248924 2314 32-33 -0.19633940733152855 2314 34-35 -0.27732352455101505 2314 36-37 -0.04313896354091895 2314 38-39 -1.5631177760726658 2314 40-41 -4.232979195908435 2314 42-43 -5.716104403041825 2314 44-45 -2.596027473098502 2314 46-47 -3.368868494534844 2314 48-49 -1.2722740672869008 2314 50-51 -0.9159706062580142 2314 52-53 -2.6196200865800776 2314 54-55 -1.477176125705249 2314 56-57 -3.9686250722689493 2314 58-59 -4.973226288331883 2314 60-61 -2.5928296077443136 2314 62-63 -1.8077092744570074 2314 64-65 -0.7630209954553813 2314 66-67 -0.6806292435436525 2314 68-69 1.2538707401320615 2314 70-71 1.2154398658848748 2314 72-73 -0.6677108208859899 2314 74-75 -0.32430074456275193 2314 76-77 -0.405846278926834 2314 78-79 -0.07672153485331279 2314 80-81 0.3455919427297509 2314 82-83 0.28411824826630294 2314 84-85 0.15851961122082514 2314 86-87 -0.06337240634764285 2314 88-89 0.12024897484959496 2314 90-91 0.702769756912879 2314 92-93 0.5965849262051819 2314 94-95 0.5012969511897651 2314 96-97 0.4326691270345613 2314 98-99 -0.0035585361190904052 2314 100-101 0.20432830388414658 2315 1 -0.3656591139023071 2315 2 -0.22023197043339593 2315 3 -0.3163707566915832 2315 4 -0.09636956069999769 2315 5 -1.1896162671839434 2315 6 -0.36056591379939107 2315 7 -0.12347464803512054 2315 8 -0.04009651684273763 2315 9 0.2003174263733527 2315 10-11 -1.7034276052939887 2315 12-13 -1.3027500806044046 2315 14-15 -0.25588383679249915 2315 16-17 -1.6903570674372617 2315 18-19 -3.034573070837986 2315 20-21 -1.9826103338453365 2315 22-23 -1.3944341212899474 2315 24-25 -0.8557648413410348 2315 26-27 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92-93 -4.294116473442937 2315 94-95 -2.3562684003988537 2315 96-97 -1.4720257238197938 2315 98-99 -2.8419366491696145 2315 100-101 -2.176716755937729 2316 1 -0.45604918801253547 2316 2 -0.42702134068911946 2316 3 -0.8746442581108518 2316 4 -0.38456458491105394 2316 5 -0.3319669870935229 2316 6 -2.4807349416324698 2316 7 -0.659226089780887 2316 8 -0.5575002185009055 2316 9 -0.23404300364578745 2316 10-11 -0.08961317627669274 2316 12-13 -0.733636192322038 2316 14-15 -1.0111884336816743 2316 16-17 -1.5994596529465994 2316 18-19 -0.35596677981727254 2316 20-21 -4.2126217650589695 2316 22-23 -4.054246341128881 2316 24-25 -2.902719514652368 2316 26-27 -2.4246740021408257 2316 28-29 -1.735894756457867 2316 30-31 -0.3688565111579436 2316 32-33 -1.8707983703493696 2316 34-35 -2.6003552266264407 2316 36-37 -5.346772794018154 2316 38-39 -2.5876068864248296 2316 40-41 -1.9492695233515533 2316 42-43 -3.150538472975029 2316 44-45 -0.9767478221184973 2316 46-47 -0.9739381994785745 2316 48-49 -0.580831603474472 2316 50-51 -0.20539341400846212 2316 52-53 -2.872708250305948 2316 54-55 -0.9376770882665646 2316 56-57 0.1395659584684097 2316 58-59 0.10065535323353814 2316 60-61 -1.5650253673064007 2316 62-63 -0.8270367304957311 2316 64-65 0.21196791463234632 2316 66-67 0.9669693059388891 2316 68-69 3.758825454529134 2316 70-71 3.830440748771096 2316 72-73 2.350052634515336 2316 74-75 1.2193409672890283 2316 76-77 -0.21389583253094457 2316 78-79 0.08828171675203222 2316 80-81 -0.38758326065708815 2316 82-83 -0.5298068054605523 2316 84-85 -0.1358230261541209 2316 86-87 -0.2266464929910832 2316 88-89 0.12021473609132016 2316 90-91 0.4643084337894443 2316 92-93 0.48220421705374505 2316 94-95 1.1870032386316787 2316 96-97 1.1256437214452433 2316 98-99 0.16900006814546487 2316 100-101 0.11211083824133894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 38.0 4 80.0 5 164.0 6 480.0 7 1079.0 8 2411.0 9 4036.0 10 6340.0 11 9184.0 12 12064.0 13 15140.0 14 18424.0 15 22983.0 16 26695.0 17 30534.0 18 28474.0 19 24918.0 20 24496.0 21 24541.0 22 26454.0 23 29908.0 24 36039.0 25 44881.0 26 56643.0 27 70030.0 28 85491.0 29 102485.0 30 119341.0 31 139066.0 32 160868.0 33 185191.0 34 215831.0 35 246457.0 36 259059.0 37 213214.0 38 84522.0 39 4570.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.421295631121055 20.48147091810148 15.234827511521035 22.862405939256437 2 16.276628228924718 21.478546456374133 38.123441075662086 24.121384239039067 3 19.214625225383607 24.943839014465286 30.571772217303028 25.26976354284808 4 13.289110621812203 17.756261965966807 36.352569641122834 32.60205777109816 5 13.350096242366966 38.069398271882434 34.13398807014866 14.446517415601939 6 31.699151206941277 36.80370132946849 16.82831396981736 14.668833493772873 7 27.81091146095745 33.38571737913972 20.579554785636336 18.223816374266498 8 26.596616405139496 35.13921792692104 19.318993111462245 18.945172556477218 9 26.114397322624978 16.315564922270795 20.336129769503053 37.23390798560118 10-11 24.96120507603548 27.159834229150544 26.825977055359147 21.052983639454833 12-13 25.718708393810825 24.571819384383836 27.68823417169246 22.021238050112878 14-15 22.764398287406177 25.84350820171217 26.247194094681483 25.14489941620018 16-17 21.767881982261663 29.069616366882723 26.963146041977186 22.19935560887843 18-19 21.590422066192833 27.037055062356607 29.923582094416197 21.448940777034366 20-21 22.422029599487164 26.15433926423642 30.147032654627626 21.27659848164879 22-23 22.182463708147257 26.214863204745864 29.991102573393047 21.61157051371383 24-25 21.907265231215174 26.45828822087915 30.07062198371878 21.56382456418689 26-27 21.78433556034566 26.608888124682963 29.976077591104755 21.630698723866622 28-29 21.656430101698053 26.631673003451688 30.135063474708218 21.576833420142037 30-31 21.746682140968083 26.528978831181387 29.992665215432762 21.73167381241776 32-33 21.591422463117265 26.591676542157934 29.943697371064538 21.87320362366027 34-35 22.072753852332283 26.56973414527828 29.773944903613163 21.58356709877627 36-37 21.97556882320876 26.424493842845553 29.734892544885632 21.86504478906005 38-39 21.836811692988654 26.71229554567939 29.848270492218436 21.60262226911352 40-41 22.022449577101035 26.46503517217753 29.8679315428278 21.644583707893627 42-43 21.84251777883846 26.733887444757688 29.480056967828116 21.943537808575737 44-45 21.91488841170217 26.833962913635546 29.390684157281623 21.860464517380667 46-47 22.030428550550184 27.029380185623904 28.887314612704813 22.052876651121107 48-49 22.180761028356837 27.23459607671277 28.617316280614 21.967326614316395 50-51 22.04428618581587 27.319328989900853 28.514581288273117 22.121803536010155 52-53 22.361694001733653 27.238514551524673 28.339062111108248 22.06072933563343 54-55 22.087102294705087 27.290752249040967 28.421617653274073 22.20052780297987 56-57 22.116611871630983 27.589523878360385 28.2489087998847 22.044955450123936 58-59 22.10892697545721 27.314084958855183 28.31118415071165 22.265803914975958 60-61 22.189409504009785 27.22176265834048 28.528389255039087 22.060438582610644 62-63 22.148847189401064 27.216998708879576 28.54452435869564 22.089629743023725 64-65 22.008003385915945 27.14283753992795 28.48511136087149 22.36404771328462 66-67 21.594834482807627 27.353561812659986 28.915069544504973 22.13653416002741 68-69 21.72242153852873 26.793062970907748 29.435865302681506 22.048650187882014 70-71 21.282525869629016 26.810462818116576 29.74819044617914 22.15882086607527 72-73 21.961468761046802 27.24563439058647 28.443591329245603 22.349305519121124 74-75 21.71291535402594 27.465787237642747 28.380031526997975 22.44126588133334 76-77 21.924789458730697 27.496523589735343 28.176109529706288 22.402577421827672 78-79 21.78860620666809 27.56955351313452 28.13590902804654 22.505931252150848 80-81 21.739784819902123 27.622890682318413 28.0439930997606 22.59333139801886 82-83 21.86830951038426 27.730169994447145 27.773327873386407 22.628192621782187 84-85 21.80392644508144 27.60759990309309 27.786749051834477 22.801724599990994 86-87 21.78346289649619 27.604704108666255 27.943664411719492 22.66816858311806 88-89 21.784708532052953 27.495012579769245 27.76378119884611 22.95649768933169 90-91 21.66777513204031 27.686034579831592 27.95884141569681 22.687348872431286 92-93 21.74876350369314 27.632211144192976 27.823927299655864 22.795098052458016 94-95 21.314334956526224 27.42191697217936 28.25060493662671 23.013143134667704 96-97 21.32308805220812 27.244900086373104 28.233151066532646 23.198860794886127 98-99 21.4258742288896 27.290826957133884 27.75268372899971 23.530615084976798 100-101 21.27329242487364 27.277654859904 28.00948732061453 23.43956539460783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3371.0 1 2263.0 2 1239.5 3 1387.5 4 1326.0 5 1278.5 6 1900.0 7 3269.5 8 4829.5 9 5752.0 10 5261.5 11 4248.0 12 3842.5 13 3705.5 14 3682.0 15 3779.5 16 3831.5 17 3940.5 18 4207.5 19 4641.5 20 5150.5 21 5976.5 22 7111.5 23 8282.0 24 9639.0 25 11714.5 26 14725.0 27 18033.5 28 20866.5 29 24167.5 30 28064.5 31 32117.0 32 36854.5 33 41339.5 34 45608.0 35 50450.0 36 56799.5 37 62313.5 38 64862.0 39 65694.0 40 67106.5 41 69667.5 42 71745.0 43 74283.0 44 77295.0 45 79838.5 46 80904.0 47 82235.0 48 85548.0 49 86550.0 50 84744.0 51 83058.0 52 80596.0 53 78939.0 54 77374.5 55 72818.5 56 67030.5 57 60948.0 58 54614.5 59 47798.0 60 40278.0 61 32486.5 62 25542.5 63 19781.0 64 14836.0 65 10932.0 66 8027.5 67 6034.5 68 4376.0 69 2997.0 70 2087.5 71 1523.0 72 1147.5 73 926.0 74 745.0 75 530.0 76 368.5 77 239.5 78 134.0 79 82.5 80 49.0 81 27.5 82 19.5 83 11.5 84 9.0 85 5.5 86 2.0 87 1.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022725957116547714 2 0.017108786583967053 3 0.0 4 0.0 5 8.575832874168948E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.7167080807929216E-4 18-19 0.0010505395270856961 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.145499724501369E-4 28-29 0.01627264287873558 30-31 0.004137839361786517 32-33 0.00786832666205001 34-35 0.008725909949466905 36-37 0.09969405716221402 38-39 0.10278135699691485 40-41 0.11736027288300206 42-43 0.10286711532565652 44-45 0.09759297810804263 46-47 0.09932958426506185 48-49 0.10203097162042507 50-51 0.10007996964155164 52-53 0.0769681000456663 54-55 0.06562656106957787 56-57 0.03736919174919119 58-59 0.015908169981583398 60-61 0.009176141175360775 62-63 0.0022297165472839266 64-65 0.005231258053243058 66-67 0.4406477326569859 68-69 0.8424255028118012 70-71 0.9389250622283873 72-73 0.2916211968861151 74-75 0.15670190619325208 76-77 0.014900509618868548 78-79 0.008897426606950283 80-81 0.001800924903575479 82-83 0.0 84-85 0.0 86-87 1.500770752979566E-4 88-89 7.503853764897829E-4 90-91 0.003516091478409269 92-93 0.0017794853213900568 94-95 0.23347704999924962 96-97 0.24014476005891597 98-99 0.015072026276351926 100-101 0.007739689168937475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2332135.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.77045122078463 #Duplication Level Percentage of deduplicated Percentage of total 1 73.9424637365595 39.0197717575475 2 14.352995043506619 15.148280496310592 3 4.967955255519136 7.864837214352398 4 2.3092976629282753 4.87450718703314 5 1.244720110073104 3.2842220926071204 6 0.7210299438268606 2.2829445287664254 7 0.484510559963815 1.7897488609367882 8 0.3201213811645178 1.3514359783577912 9 0.25056715567957405 1.190028767968774 >10 1.2541247107261384 12.149261657026718 >50 0.09851100890072677 3.614484359620941 >100 0.05083674962712134 4.774803947158625 >500 0.0019657244739301697 0.6045856493753385 >1k 6.552414913097211E-4 0.6680351584744805 >5k 8.190518641371514E-5 0.33064628379462835 >10k+ 1.6381037282743027E-4 1.0524060606686805 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12448 0.5337598380882753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11901 0.5103049351774233 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7650 0.32802560743696224 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3860 0.1655135744714607 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3012 0.12915204308498437 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2641 0.11324387310340095 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.287916437084474E-5 6 0.0 0.0 0.0 0.0 4.287916437084474E-5 7 0.0 0.0 0.0 0.0 4.287916437084474E-5 8 0.0 0.0 0.0 0.0 4.287916437084474E-5 9 0.0 0.0 0.0 0.0 4.287916437084474E-5 10-11 0.0 0.0 0.0 2.143958218542237E-5 4.287916437084474E-5 12-13 0.0 0.0 0.0 4.287916437084474E-5 4.287916437084474E-5 14-15 0.0 0.0 0.0 4.287916437084474E-5 4.287916437084474E-5 16-17 0.0 0.0 0.0 6.431874655626711E-5 4.287916437084474E-5 18-19 0.0 0.0 0.0 8.575832874168948E-5 4.287916437084474E-5 20-21 0.0 0.0 0.0 1.9295623966880134E-4 4.287916437084474E-5 22-23 0.0 0.0 0.0 6.217478833772487E-4 4.287916437084474E-5 24-25 0.0 0.0 0.0 0.00126493534893992 4.287916437084474E-5 26-27 0.0 0.0 0.0 0.0020796394719859697 4.287916437084474E-5 28-29 0.0 0.0 0.0 0.003666168553707225 4.287916437084474E-5 30-31 0.0 0.0 0.0 0.008275678723573035 4.287916437084474E-5 32-33 2.143958218542237E-5 0.0 0.0 0.015886730399397975 4.287916437084474E-5 34-35 4.287916437084474E-5 0.0 0.0 0.028900556785949355 4.287916437084474E-5 36-37 4.287916437084474E-5 0.0 0.0 0.0524197784433577 4.287916437084474E-5 38-39 4.287916437084474E-5 0.0 0.0 0.08472922879678921 4.287916437084474E-5 40-41 4.287916437084474E-5 0.0 0.0 0.12177682681319905 4.287916437084474E-5 42-43 4.287916437084474E-5 0.0 0.0 0.15648751037139788 4.287916437084474E-5 44-45 4.287916437084474E-5 0.0 0.0 0.20061017050899713 4.287916437084474E-5 46-47 4.287916437084474E-5 0.0 0.0 0.2547451155271886 4.287916437084474E-5 48-49 4.287916437084474E-5 0.0 0.0 0.3156978476803444 4.287916437084474E-5 50-51 4.287916437084474E-5 0.0 0.0 0.3799951546544261 4.287916437084474E-5 52-53 4.287916437084474E-5 0.0 0.0 0.44358495541638887 8.575832874168948E-5 54-55 4.287916437084474E-5 0.0 0.0 0.5173156785520564 8.575832874168948E-5 56-57 6.431874655626711E-5 0.0 0.0 0.6018733906913623 8.575832874168948E-5 58-59 8.575832874168948E-5 0.0 0.0 0.6857879153651054 8.575832874168948E-5 60-61 8.575832874168948E-5 0.0 0.0 0.7689949338267296 8.575832874168948E-5 62-63 8.575832874168948E-5 0.0 2.143958218542237E-5 0.852008996048685 8.575832874168948E-5 64-65 8.575832874168948E-5 0.0 4.287916437084474E-5 0.9414120537618963 8.575832874168948E-5 66-67 8.575832874168948E-5 0.0 4.287916437084474E-5 1.0304506385779555 8.575832874168948E-5 68-69 8.575832874168948E-5 0.0 4.287916437084474E-5 1.1412718388944036 8.575832874168948E-5 70-71 8.575832874168948E-5 0.0 4.287916437084474E-5 1.2931927182603065 8.575832874168948E-5 72-73 8.575832874168948E-5 0.0 4.287916437084474E-5 1.4613004821762035 8.575832874168948E-5 74-75 8.575832874168948E-5 0.0 4.287916437084474E-5 1.64589528479269 8.575832874168948E-5 76-77 8.575832874168948E-5 0.0 4.287916437084474E-5 1.850922009231884 1.2863749311253423E-4 78-79 8.575832874168948E-5 0.0 4.287916437084474E-5 2.0896946360309334 1.2863749311253423E-4 80-81 8.575832874168948E-5 0.0 4.287916437084474E-5 2.349906845015404 1.2863749311253423E-4 82-83 8.575832874168948E-5 0.0 4.287916437084474E-5 2.6211389992431826 1.2863749311253423E-4 84-85 8.575832874168948E-5 0.0 4.287916437084474E-5 2.8818871977822895 1.2863749311253423E-4 86-87 8.575832874168948E-5 0.0 4.287916437084474E-5 3.1546630019274184 1.500770752979566E-4 88-89 8.575832874168948E-5 0.0 4.287916437084474E-5 3.4589764314673035 1.7151665748337897E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6185 0.0 56.5529 1 GTATCAA 10335 0.0 36.595745 1 ATCAACG 13640 0.0 27.368715 3 TATCAAC 13795 0.0 27.100775 2 TCAACGC 13925 0.0 26.808567 4 GTGGTAT 2880 0.0 26.495466 1 CAACGCA 14100 0.0 26.40864 5 AACGCAG 14530 0.0 25.659708 6 TGGTATC 2875 0.0 25.545843 2 GTACATG 10725 0.0 23.730951 1 TACATGG 11105 0.0 21.846292 2 ACGCAGA 16975 0.0 21.824263 7 CGCAGAG 17060 0.0 21.687754 8 ACATGGG 11105 0.0 21.244835 3 GAGTACT 10765 0.0 21.101688 12-13 GGACCGT 225 7.362905E-8 21.055235 6 GCAGAGT 18160 0.0 19.774065 9 CATGGGA 7000 0.0 19.694164 4 CAGAGTA 17625 0.0 19.460411 10-11 GTACTTT 11765 0.0 19.42889 14-15 >>END_MODULE