FastQCFastQC Report
Fri 27 May 2016
SRR939402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314365
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85680.3702095391176414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67540.29182950831005483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46330.20018449985201126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24730.10685436394000081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.049.8016621
GTATCAA92850.041.41361
TATCAAC122750.031.756322
ATCAACG124750.031.2291073
TCAACGC127550.030.4658854
CAACGCA128450.030.0380655
AACGCAG131250.029.4436826
GTACATG95950.026.3861451
ACGCAGA148600.025.256967
CGCAGAG150100.024.9077668
TACATGG98800.024.671322
ACATGGG103050.023.383253
GCAGAGT152150.022.7584089
GAGTACT88150.021.71345112-13
GTGGTAT30050.021.3547781
TGGTATC29700.020.8879622
AGAGTAC137900.020.84319510-11
CATGGGG44500.020.464924
AGTACTT95450.019.82636612-13
CAGAGTA148300.019.69173410-11
Produced by FastQC (version 0.11.5)