FastQCFastQC Report
Fri 27 May 2016
SRR939402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314365
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121120.5233400954473474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112760.48721787617769885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.327001142862081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38040.1643647393561517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29300.12660060102879192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.11126162035806797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64150.050.5956881
GTATCAA106500.036.1793251
ATCAACG138050.027.7655893
TATCAAC139000.027.545272
TCAACGC139550.027.4062674
TGGTATC33200.027.295032
GTGGTAT34250.026.8778921
CAACGCA142100.026.8227235
AACGCAG146100.026.1857036
GTACATG110150.023.5213471
TACATGG112800.022.5026152
ACGCAGA171050.022.1998657
CGCAGAG171900.022.0900948
GAGTACT105700.021.00739912-13
ACATGGG114550.020.8307483
GCAGAGT183150.019.93089
CATGGGA67900.019.8469244
AGCGTAC1200.004792909619.7716431
AGTACTT112400.019.64976312-13
ATAACGC2451.7820275E-719.3626963
Produced by FastQC (version 0.11.5)