##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939402_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2314365 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.844036269127816 31.0 31.0 31.0 28.0 34.0 2 31.09978158155693 31.0 31.0 34.0 28.0 34.0 3 31.09624151765171 31.0 31.0 34.0 27.0 34.0 4 35.02281057655124 35.0 35.0 37.0 32.0 37.0 5 34.58716969881588 35.0 35.0 37.0 30.0 37.0 6 34.645572759698666 35.0 35.0 37.0 30.0 37.0 7 34.486599996111245 35.0 35.0 37.0 30.0 37.0 8 34.38951029764104 35.0 35.0 37.0 30.0 37.0 9 35.88357972921298 37.0 35.0 39.0 30.0 39.0 10-11 35.68099975587256 37.0 35.0 39.0 30.0 39.0 12-13 35.66191050244883 37.0 35.0 39.0 30.0 39.0 14-15 36.48122984058262 38.0 35.0 40.0 30.0 41.0 16-17 36.38697850166244 38.0 35.0 40.0 30.0 41.0 18-19 36.38566280599646 38.0 35.0 40.0 30.0 41.0 20-21 36.268353522456486 38.0 34.0 40.0 29.5 41.0 22-23 36.104372689701066 38.0 34.0 40.0 29.0 41.0 24-25 35.9297807389932 38.0 34.0 40.0 29.0 41.0 26-27 35.455405694434546 38.0 34.0 40.0 27.0 41.0 28-29 35.230525867786625 38.0 33.5 40.0 27.0 41.0 30-31 34.92532876188501 37.0 33.0 40.0 26.5 41.0 32-33 34.67433378054024 37.0 33.0 40.0 25.0 41.0 34-35 34.91375582503192 37.0 33.0 40.0 26.0 41.0 36-37 34.75151888314937 37.5 33.0 40.0 25.0 41.0 38-39 34.55891075953879 37.0 33.0 40.0 25.0 41.0 40-41 34.394099461407336 37.0 33.0 40.0 24.5 41.0 42-43 34.22070416723378 37.0 33.0 40.0 24.0 41.0 44-45 33.834723131398896 37.0 32.5 40.0 23.5 41.0 46-47 33.421096067387815 37.0 32.0 40.0 22.5 41.0 48-49 33.021824344906705 37.0 31.0 39.5 20.5 41.0 50-51 32.76496490398014 36.0 31.0 39.0 19.0 40.0 52-53 32.644909294774166 36.0 31.0 39.0 19.0 40.0 54-55 32.29910169744185 36.0 30.5 39.0 18.0 40.0 56-57 31.801618370481755 35.5 30.0 39.0 14.5 40.0 58-59 31.335184813112882 35.0 29.0 38.5 13.0 40.0 60-61 30.957191713493767 35.0 28.0 38.0 9.0 40.0 62-63 30.775216095991773 34.0 28.0 38.0 8.5 40.0 64-65 30.29922873012684 34.0 28.0 38.0 8.0 40.0 66-67 29.39337572077006 33.5 27.0 37.0 4.5 39.5 68-69 25.898059943008125 31.0 17.0 35.5 2.0 39.0 70-71 24.53894307941919 29.5 12.0 35.0 2.0 38.0 72-73 25.577497499314067 30.0 18.5 35.0 2.0 37.5 74-75 26.34667262942535 31.0 21.5 35.0 2.0 37.0 76-77 26.035180492273255 30.5 22.0 34.0 2.0 36.0 78-79 27.185597777360094 32.0 25.5 35.0 2.0 37.0 80-81 27.204713603947518 32.0 25.5 35.0 2.0 36.0 82-83 26.876096034981515 32.0 25.0 35.0 2.0 36.0 84-85 26.5113108779298 32.0 24.5 34.5 2.0 35.5 86-87 26.056289090095987 31.5 23.5 34.0 2.0 35.0 88-89 25.561134047568125 31.0 22.0 34.0 2.0 35.0 90-91 24.94936429647009 31.0 20.0 34.0 2.0 35.0 92-93 24.365031012826414 31.0 18.0 34.0 2.0 35.0 94-95 23.490334497799612 30.0 7.0 34.0 2.0 35.0 96-97 22.80688352960747 30.0 2.0 34.0 2.0 35.0 98-99 21.932596846219155 29.5 2.0 34.0 2.0 35.0 100-101 20.512122547653462 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3266392962620124 1101 2 0.23564317369686094 1101 3 0.36598580051302676 1101 4 0.36222992155941114 1101 5 0.46045307507302624 1101 6 0.28625351484479467 1101 7 0.7088027072521044 1101 8 0.6380463782357282 1101 9 0.7395078529280852 1101 10-11 0.727948059228325 1101 12-13 0.8427674818051685 1101 14-15 0.7878271086677131 1101 16-17 0.8336702128161022 1101 18-19 0.5617487150802489 1101 20-21 0.7031508012732104 1101 22-23 0.8744584615134627 1101 24-25 0.8145536776057796 1101 26-27 0.8040934957021122 1101 28-29 0.5697843202196609 1101 30-31 0.5079889076997972 1101 32-33 0.833042990924163 1101 34-35 0.8103348016053431 1101 36-37 1.0728407080413191 1101 38-39 1.0245634876486776 1101 40-41 1.0164903743484146 1101 42-43 0.6338669665314995 1101 44-45 0.9761549424822888 1101 46-47 0.8475160209428907 1101 48-49 1.03287787791934 1101 50-51 0.5692580726525307 1101 52-53 0.37778418521611457 1101 54-55 0.41966121339008566 1101 56-57 0.6963109853073526 1101 58-59 0.5595820306736172 1101 60-61 0.6385935892438539 1101 62-63 0.31682819066308454 1101 64-65 0.0712617629545278 1101 66-67 -0.1344187475720453 1101 68-69 -7.02423541519811 1101 70-71 -7.730701539919245 1101 72-73 -5.028453880803326 1101 74-75 -2.953429735680711 1101 76-77 -0.1980868100466786 1101 78-79 0.07766274234658965 1101 80-81 0.061060116864759095 1101 82-83 -0.3183457059783237 1101 84-85 -0.3931781324554997 1101 86-87 -0.3636740304308006 1101 88-89 -0.40522841747719696 1101 90-91 -0.43219250766474815 1101 92-93 -0.23900146678326806 1101 94-95 -0.27880858920671514 1101 96-97 -0.5792362988041226 1101 98-99 -0.47031056125997495 1101 100-101 -0.3450572343865055 1102 1 0.3215899229104018 1102 2 0.3289491353811904 1102 3 0.39368289378307963 1102 4 0.3601089899027059 1102 5 0.5007442351902114 1102 6 0.4591630979374628 1102 7 0.5292116118526309 1102 8 0.576837200846569 1102 9 0.7019867500825967 1102 10-11 0.6579151436605741 1102 12-13 0.7168453239913788 1102 14-15 0.8779037956775468 1102 16-17 0.5404523507274632 1102 18-19 0.5886903508378296 1102 20-21 0.6946618554760278 1102 22-23 0.7820715609351723 1102 24-25 0.5684127321704651 1102 26-27 0.690192826952412 1102 28-29 0.5167534436078043 1102 30-31 0.6008276492534392 1102 32-33 0.6801725168147286 1102 34-35 0.525924950595261 1102 36-37 0.6221351457974933 1102 38-39 0.9498296305425953 1102 40-41 0.738308107001636 1102 42-43 0.6949709254461922 1102 44-45 0.6350030367892217 1102 46-47 0.6214885054767478 1102 48-49 0.7322584667809409 1102 50-51 0.4552633892474063 1102 52-53 0.15646561401619863 1102 54-55 -0.043953305645970886 1102 56-57 0.2570652598078773 1102 58-59 0.306502511394001 1102 60-61 0.46131611727080113 1102 62-63 0.24518784021319107 1102 64-65 0.13848279829874954 1102 66-67 -0.012394614128297121 1102 68-69 -6.842808911555132 1102 70-71 -7.605936715735698 1102 72-73 -1.616461619093247 1102 74-75 -0.030185081850884643 1102 76-77 0.43015050215017325 1102 78-79 0.1447679121088079 1102 80-81 -0.16669772754829282 1102 82-83 -0.26980637778993355 1102 84-85 -0.5169548633063634 1102 86-87 -0.6631091074309623 1102 88-89 -0.7091588007034808 1102 90-91 -0.5105365692927109 1102 92-93 -0.44363024459274314 1102 94-95 -0.17364362862884875 1102 96-97 -0.5742730765579331 1102 98-99 -0.6066179634353226 1102 100-101 -0.48472550391838354 1103 1 0.4361602159297959 1103 2 0.28160020731744595 1103 3 0.46120886849977794 1103 4 0.3986968991745883 1103 5 0.40494425812228485 1103 6 0.4367579103158121 1103 7 0.3969243586314306 1103 8 0.48111748603313487 1103 9 0.5972027881664133 1103 10-11 0.5357177261435169 1103 12-13 0.540320582909672 1103 14-15 0.6767938695436868 1103 16-17 0.8162755914161437 1103 18-19 0.7626697699932876 1103 20-21 0.706927958695232 1103 22-23 0.7429008869118334 1103 24-25 0.7334880142971301 1103 26-27 0.9409717576271035 1103 28-29 0.7204229206045838 1103 30-31 0.6226051059207975 1103 32-33 0.7354649214635813 1103 34-35 0.6225460616403709 1103 36-37 0.9669745314942304 1103 38-39 1.0831500012685638 1103 40-41 0.9619802916407352 1103 42-43 0.6502611197747825 1103 44-45 0.811095729332294 1103 46-47 0.9735170959693349 1103 48-49 0.9317217043199122 1103 50-51 0.8776138023700781 1103 52-53 0.7450895100648296 1103 54-55 0.49004839630062946 1103 56-57 0.8031998241053948 1103 58-59 0.8133708053168647 1103 60-61 0.6170774551776539 1103 62-63 0.40815827579824315 1103 64-65 0.40879301674746316 1103 66-67 0.21156325440461643 1103 68-69 -6.656948123051002 1103 70-71 -7.773748719708749 1103 72-73 -1.0502543767026182 1103 74-75 0.6030115800129394 1103 76-77 0.9563206249749108 1103 78-79 0.7356957907401203 1103 80-81 0.43189326235745185 1103 82-83 0.4235005652404418 1103 84-85 0.12138466122612002 1103 86-87 0.09857416196425461 1103 88-89 0.20443121527990016 1103 90-91 0.13482914891632092 1103 92-93 0.16264609793505969 1103 94-95 0.3319170536417566 1103 96-97 0.18501144277339066 1103 98-99 0.5666793647019155 1103 100-101 0.6634777819989779 1104 1 0.25990014916157733 1104 2 0.30158238770231094 1104 3 0.2631033094162696 1104 4 0.17716186661366606 1104 5 0.30726191489823407 1104 6 0.4238050700379503 1104 7 0.529876811526357 1104 8 0.6120869533400963 1104 9 0.44096972752299735 1104 10-11 0.5460702584519481 1104 12-13 0.41360713975328167 1104 14-15 0.6518065632524497 1104 16-17 0.5057405048983199 1104 18-19 0.5042799548441295 1104 20-21 0.48494155583634324 1104 22-23 0.5656808022133148 1104 24-25 0.5304223625737521 1104 26-27 0.7401805279768183 1104 28-29 0.6862141710075278 1104 30-31 0.7097736686885909 1104 32-33 0.6956024562415806 1104 34-35 0.4945604805867134 1104 36-37 0.7656694660472709 1104 38-39 0.8073698742899751 1104 40-41 0.7115997570799593 1104 42-43 0.6569798617763709 1104 44-45 0.6008129951281731 1104 46-47 0.6728238188618718 1104 48-49 0.6660469455003764 1104 50-51 0.504234411972476 1104 52-53 0.30849289053436735 1104 54-55 0.4696957809815743 1104 56-57 0.4964873079010381 1104 58-59 0.6765605025909309 1104 60-61 0.6380454559634892 1104 62-63 0.2942879605377051 1104 64-65 0.3654923204266325 1104 66-67 0.45173804712015553 1104 68-69 -6.322615825967027 1104 70-71 -4.517382842058119 1104 72-73 0.22347068379137625 1104 74-75 1.0212955704445825 1104 76-77 1.1672822477773757 1104 78-79 0.7486413675879042 1104 80-81 0.4648868746895083 1104 82-83 0.42025291271187015 1104 84-85 0.4412313959338334 1104 86-87 0.38236087168576915 1104 88-89 0.42852864762277676 1104 90-91 0.48411740194378794 1104 92-93 0.5019676105547504 1104 94-95 0.6167514266832264 1104 96-97 0.3953355439412114 1104 98-99 0.5169156682939224 1104 100-101 0.6992942399630877 1105 1 0.11369049831486322 1105 2 0.17328968812920564 1105 3 0.18285310145040867 1105 4 0.13343197440907062 1105 5 0.384991078732817 1105 6 0.24680332519457693 1105 7 0.26361533564284656 1105 8 0.5185848306058318 1105 9 0.5083489884456114 1105 10-11 0.442483819336573 1105 12-13 0.4944432044741518 1105 14-15 0.4737538006210755 1105 16-17 0.3847937413617899 1105 18-19 0.5555157783413875 1105 20-21 0.6292645493363977 1105 22-23 0.5831593049486159 1105 24-25 0.5644926810482289 1105 26-27 0.5948571445582473 1105 28-29 0.549937372501418 1105 30-31 0.6281967867723708 1105 32-33 0.6161873284104047 1105 34-35 0.5303894881741869 1105 36-37 0.7154566891394012 1105 38-39 0.558274742565203 1105 40-41 0.7615042913790759 1105 42-43 0.5798320793050138 1105 44-45 0.7239045901745911 1105 46-47 0.6782801605759374 1105 48-49 0.7111044906472941 1105 50-51 0.5069143325118972 1105 52-53 0.4046598293607957 1105 54-55 0.4802530903312814 1105 56-57 0.6318400778608613 1105 58-59 0.6543936073995731 1105 60-61 0.7774978246485027 1105 62-63 0.3961378077869888 1105 64-65 0.2779042880717846 1105 66-67 0.6865997547170792 1105 68-69 -2.802377824632245 1105 70-71 -3.0337283713934724 1105 72-73 0.6909247956414859 1105 74-75 1.2300758136038041 1105 76-77 1.3466683974467841 1105 78-79 0.8900578738397478 1105 80-81 0.5911978148406547 1105 82-83 0.6554355898129103 1105 84-85 0.7474028772829193 1105 86-87 0.6636922503917404 1105 88-89 0.4967259262489243 1105 90-91 0.5494070744737805 1105 92-93 0.7052696944649917 1105 94-95 0.788733600078924 1105 96-97 0.7925504796661933 1105 98-99 0.45117873629806127 1105 100-101 0.7220279714944056 1106 1 0.10189091380334858 1106 2 0.11489191560915302 1106 3 -0.007705788272019731 1106 4 0.2376936167427317 1106 5 0.2471784524993339 1106 6 0.1774900353336477 1106 7 0.2716567690781275 1106 8 0.33995507701358463 1106 9 0.36697729936949486 1106 10-11 0.2931799068199865 1106 12-13 0.227278624867715 1106 14-15 0.1643673674743269 1106 16-17 0.28032190224816844 1106 18-19 0.23413009538219143 1106 20-21 0.2307766100438755 1106 22-23 0.21457528394336833 1106 24-25 0.2478786236877326 1106 26-27 0.4730546747100206 1106 28-29 0.3550664913195831 1106 30-31 0.3830985410570875 1106 32-33 0.5992058234187709 1106 34-35 0.34926362978651326 1106 36-37 0.4035272644755423 1106 38-39 0.5100577363905856 1106 40-41 0.4918336525655249 1106 42-43 0.29355603914113004 1106 44-45 0.41596443205813927 1106 46-47 0.6448147269098286 1106 48-49 0.6488142546267497 1106 50-51 0.35461572901486704 1106 52-53 0.4010228624267569 1106 54-55 0.3249110336632697 1106 56-57 0.4363127825755022 1106 58-59 0.32723479846657 1106 60-61 0.5710252930540634 1106 62-63 0.4810571626256994 1106 64-65 0.4932135961680437 1106 66-67 0.5681944803215551 1106 68-69 -3.2103497214526016 1106 70-71 -3.1201551704009134 1106 72-73 0.9085446813821534 1106 74-75 1.373576015577374 1106 76-77 1.424853520650661 1106 78-79 1.1541544749130885 1106 80-81 0.8335187796972541 1106 82-83 0.6590931869044923 1106 84-85 0.535504469988787 1106 86-87 0.5973468178797674 1106 88-89 0.6187147888491857 1106 90-91 0.6024372550958574 1106 92-93 0.46339249439574104 1106 94-95 0.73301024616352 1106 96-97 0.7306468787659703 1106 98-99 0.6542742697968187 1106 100-101 0.866766992788186 1107 1 0.037868187235279294 1107 2 0.0017109712008824829 1107 3 0.10327970144986409 1107 4 -0.10967717047086012 1107 5 0.27306240127929726 1107 6 0.14545921649509097 1107 7 -0.033088525933187896 1107 8 -0.08522785170622171 1107 9 0.3279672847972037 1107 10-11 0.09700862042801361 1107 12-13 0.08273749691062449 1107 14-15 0.04063864456482946 1107 16-17 -7.024210705921519E-4 1107 18-19 0.1296335369225048 1107 20-21 0.15130051479692241 1107 22-23 0.1413719385181409 1107 24-25 0.295741923225755 1107 26-27 0.5214844331820956 1107 28-29 0.20429904333025206 1107 30-31 0.3437852213658985 1107 32-33 0.1734247332876251 1107 34-35 0.15964126907587683 1107 36-37 0.26004371255788783 1107 38-39 0.48204460582908837 1107 40-41 0.4379233961552771 1107 42-43 0.19347286858335622 1107 44-45 0.36988546265994415 1107 46-47 0.5357034039321178 1107 48-49 0.2927424938603096 1107 50-51 0.34802417164036115 1107 52-53 0.17209609592441666 1107 54-55 0.14028735524575353 1107 56-57 0.6909766122967689 1107 58-59 0.5345440410176501 1107 60-61 0.66874837307763 1107 62-63 0.44911137463698836 1107 64-65 0.26853170611130395 1107 66-67 0.3776979373643137 1107 68-69 -2.9333610692342056 1107 70-71 -2.889561737231908 1107 72-73 0.8719683939050213 1107 74-75 1.2608344101453248 1107 76-77 0.8958301739852352 1107 78-79 0.6841839295374648 1107 80-81 0.5460723406063188 1107 82-83 0.4820072569134837 1107 84-85 0.4746198235993049 1107 86-87 0.5780586896436333 1107 88-89 0.5024429691734973 1107 90-91 0.5641006662514094 1107 92-93 0.6223367786483713 1107 94-95 0.8060626753949087 1107 96-97 0.8843137469642741 1107 98-99 1.016722026594639 1107 100-101 0.9107194158186047 1108 1 -0.02366586867822207 1108 2 0.08443917990975436 1108 3 0.1333031078056166 1108 4 0.14261289883548756 1108 5 0.03138807021684187 1108 6 0.004266295870529291 1108 7 0.25484909232302044 1108 8 0.2846804183927887 1108 9 0.18033790156552243 1108 10-11 0.37342462671795573 1108 12-13 0.2923442567691694 1108 14-15 0.29438564175968907 1108 16-17 0.45925569490112395 1108 18-19 0.20385520347263508 1108 20-21 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4.048076098494509 2311 70-71 5.329427258458068 2311 72-73 4.11323919928088 2311 74-75 3.0470221615299344 2311 76-77 2.743474115038314 2311 78-79 2.037059317633222 2311 80-81 1.6839434550731873 2311 82-83 1.5688487814894891 2311 84-85 1.634602133484858 2311 86-87 1.7215762057315267 2311 88-89 1.8690469118313864 2311 90-91 2.112970072714358 2311 92-93 2.052825734321541 2311 94-95 2.332672400325418 2311 96-97 2.3046219942097856 2311 98-99 2.365262054459567 2311 100-101 2.49323530000688 2312 1 -0.19470890740027613 2312 2 -0.1276423198331642 2312 3 -0.03685792773319463 2312 4 0.055209566192118587 2312 5 -1.1316156878558203 2312 6 -0.27054808080271897 2312 7 0.09772243611051579 2312 8 0.2016510012689423 2312 9 -2.7850430996715048 2312 10-11 -0.11910675659584058 2312 12-13 -1.1957741200630068 2312 14-15 -4.101754478191886 2312 16-17 -0.9666205781451822 2312 18-19 -0.04419724420902327 2312 20-21 0.22073343478279384 2312 22-23 0.355501063129843 2312 24-25 0.4467268093108814 2312 26-27 0.29916565036933207 2312 28-29 0.22983862752991513 2312 30-31 0.1276008572828644 2312 32-33 0.5668994517661261 2312 34-35 0.37784382402438865 2312 36-37 0.6697479560924222 2312 38-39 0.6326593252401977 2312 40-41 0.4738452133766131 2312 42-43 0.07989142456923304 2312 44-45 0.7164518553585566 2312 46-47 0.8068132843481663 2312 48-49 0.8679709655861529 2312 50-51 0.9975037984203681 2312 52-53 0.8586003747190105 2312 54-55 0.9723214322979103 2312 56-57 0.8202634713479036 2312 58-59 0.919610961658897 2312 60-61 0.8999786231365547 2312 62-63 1.1241382508454585 2312 64-65 1.2792981587264585 2312 66-67 1.6164896162474385 2312 68-69 4.250188902628018 2312 70-71 5.012946133999911 2312 72-73 3.113578002524825 2312 74-75 2.4209361277314656 2312 76-77 2.1501666174851515 2312 78-79 1.451062923041821 2312 80-81 1.3642786642923177 2312 82-83 1.402610497644467 2312 84-85 1.4808593198404658 2312 86-87 1.4924949344224956 2312 88-89 1.4169961086915208 2312 90-91 1.4950005449491641 2312 92-93 1.698948185403097 2312 94-95 1.924118377335045 2312 96-97 1.9462398528811278 2312 98-99 2.2584823847141244 2312 100-101 2.102500565871207 2313 1 -0.2991208654158797 2313 2 -0.0850971408594674 2313 3 -0.19755834905836522 2313 4 0.00597926280902783 2313 5 0.1350631673706033 2313 6 0.17202344295034777 2313 7 -2.722029808183205 2313 8 -3.3200204492707854 2313 9 -0.9438854488496915 2313 10-11 0.00803276606627179 2313 12-13 0.04212183390906432 2313 14-15 -0.42037108212573315 2313 16-17 -0.02598187028555543 2313 18-19 0.24069790548207237 2313 20-21 -2.47835835354401 2313 22-23 -0.5344818810850853 2313 24-25 0.1346570470233175 2313 26-27 -1.3105234621040154 2313 28-29 -1.5448432742898675 2313 30-31 -0.7986811756049335 2313 32-33 -0.6034575336587054 2313 34-35 -1.2320277069161065 2313 36-37 -1.9181956453099716 2313 38-39 -1.775277832076128 2313 40-41 -0.7083087128290302 2313 42-43 0.21880684498298564 2313 44-45 0.19716523591025492 2313 46-47 0.011300560011022753 2313 48-49 -1.911457642384672 2313 50-51 -0.3434648750680651 2313 52-53 -0.19362774046233255 2313 54-55 -0.28024943830139293 2313 56-57 -0.16697256503085 2313 58-59 0.14905759284756215 2313 60-61 0.35397377886726744 2313 62-63 0.6148142759210948 2313 64-65 0.47742128449626975 2313 66-67 1.1332028372000629 2313 68-69 2.088627356823494 2313 70-71 4.438569410760294 2313 72-73 3.6833214333420408 2313 74-75 2.3173376057719715 2313 76-77 2.30812106014686 2313 78-79 1.5751509331883895 2313 80-81 1.5123189707116218 2313 82-83 1.314257240388514 2313 84-85 1.0372648862409264 2313 86-87 1.3004468218592322 2313 88-89 0.8423331182693765 2313 90-91 1.3898060322059713 2313 92-93 1.6367973254674126 2313 94-95 1.6795214483601875 2313 96-97 1.843951327808906 2313 98-99 2.1843613206146024 2313 100-101 2.22800570105354 2314 1 -0.39411056865423433 2314 2 -0.2734666744089651 2314 3 -0.21598201953540297 2314 4 -0.20564355999915307 2314 5 -0.23991675422114156 2314 6 -2.798482621559778 2314 7 -0.7010660113534328 2314 8 -0.11486702807618343 2314 9 -0.24113174362177858 2314 10-11 -0.48285163658307795 2314 12-13 -2.533686619737786 2314 14-15 -0.8434950110465067 2314 16-17 -1.8948374619422452 2314 18-19 -0.5004346385990388 2314 20-21 -1.0155003624483 2314 22-23 -0.5706442021380056 2314 24-25 -0.424247879359541 2314 26-27 -2.195404392988877 2314 28-29 -0.9864822650632235 2314 30-31 -0.5435704591076203 2314 32-33 -0.1449915905163337 2314 34-35 -0.3373991934716045 2314 36-37 -0.7835794724705991 2314 38-39 -0.3844905258396878 2314 40-41 -0.5076511934235981 2314 42-43 -0.7305754877901833 2314 44-45 -3.688968033762979 2314 46-47 -4.075873380485994 2314 48-49 -3.901289105610445 2314 50-51 -4.4068416091501845 2314 52-53 -1.1038404220403812 2314 54-55 -1.3974860178933106 2314 56-57 -1.0492402564625465 2314 58-59 -1.599768166448598 2314 60-61 -1.2281572963051843 2314 62-63 -1.450265107181579 2314 64-65 -1.539014208147556 2314 66-67 -1.3742112189042714 2314 68-69 -1.6392321131508396 2314 70-71 1.2395153945801418 2314 72-73 1.0079121661457862 2314 74-75 0.518418710355828 2314 76-77 0.4991608835233343 2314 78-79 -1.9734760637131679 2314 80-81 -1.3636596093655022 2314 82-83 -0.26906815389603267 2314 84-85 -0.01242626629847976 2314 86-87 -0.3910941308930873 2314 88-89 -0.3206164580623607 2314 90-91 0.03650248322960792 2314 92-93 -0.6779128918668178 2314 94-95 -0.150123504454168 2314 96-97 0.39897128990087083 2314 98-99 0.12644812912806813 2314 100-101 0.2362996068550629 2315 1 -0.3288245997333945 2315 2 -0.26214933922539885 2315 3 -0.22700746424967377 2315 4 -0.16434763809181874 2315 5 -0.2238292827821411 2315 6 -0.10730154180429707 2315 7 -0.9899891269253089 2315 8 -2.8036525723203205 2315 9 -0.6659878918638569 2315 10-11 -1.4411507764632177 2315 12-13 -0.6182370605641694 2315 14-15 -0.4142649917865242 2315 16-17 -0.18567401524216365 2315 18-19 -0.10184863259375021 2315 20-21 -0.712737571305496 2315 22-23 -0.1856062803024443 2315 24-25 -4.901083559380595 2315 26-27 -1.0366165758271535 2315 28-29 -0.7885078533301808 2315 30-31 -1.195104080343853 2315 32-33 -0.22579739961518186 2315 34-35 -2.2745051712448614 2315 36-37 -3.4846656878235613 2315 38-39 -1.1545071540262626 2315 40-41 -0.26812874537173315 2315 42-43 -1.6711270291871827 2315 44-45 -5.160685761323897 2315 46-47 -3.2368279460596625 2315 48-49 -2.6083913749392167 2315 50-51 -0.9781313702545802 2315 52-53 -0.12530932493894653 2315 54-55 -1.1814200793577854 2315 56-57 -0.9976246905658819 2315 58-59 -0.3999224855901531 2315 60-61 -1.41363843707056 2315 62-63 -0.4149903234078174 2315 64-65 0.10331888194371786 2315 66-67 0.42869512492834616 2315 68-69 3.0646545759259958 2315 70-71 3.6593334415457974 2315 72-73 -2.1553165132890975 2315 74-75 -1.8162585353465595 2315 76-77 -0.17757879563640344 2315 78-79 -0.10364096986559446 2315 80-81 -0.19920146095595115 2315 82-83 -0.8857059106460028 2315 84-85 -0.4699478291087651 2315 86-87 0.08683972720508137 2315 88-89 0.2261556998934502 2315 90-91 0.37900364959521227 2315 92-93 -0.816852456184403 2315 94-95 0.10823156116137156 2315 96-97 -0.2176631770999542 2315 98-99 -2.3585437362195094 2315 100-101 -3.738818525045936 2316 1 -0.5158573071387025 2316 2 -0.42595743327984437 2316 3 -0.894115479901977 2316 4 -0.28284421546910465 2316 5 -0.24560292911464643 2316 6 -0.5448902715841015 2316 7 -0.5961628562304853 2316 8 -0.938707119820414 2316 9 -4.616810762788614 2316 10-11 -1.675608518411174 2316 12-13 -0.5954480537400215 2316 14-15 -0.3296759350381393 2316 16-17 -0.2702927804259829 2316 18-19 -0.6096079837193358 2316 20-21 -3.569878494763209 2316 22-23 -1.1656056474600334 2316 24-25 -0.42592613983068617 2316 26-27 -0.5122120629576017 2316 28-29 -0.005342692008106553 2316 30-31 0.02900053553837978 2316 32-33 -1.3370147071519796 2316 34-35 -0.748713327177235 2316 36-37 -0.42756994228246015 2316 38-39 -5.510057357766357 2316 40-41 -5.193815643072753 2316 42-43 -3.6921530425260976 2316 44-45 -2.4841548979510613 2316 46-47 -2.806705702000471 2316 48-49 -3.0365414359381937 2316 50-51 -1.5221924957999704 2316 52-53 -1.1694217810355063 2316 54-55 -0.2932115155936401 2316 56-57 -3.973262542979999 2316 58-59 -1.0186864388863839 2316 60-61 -0.6843838902736223 2316 62-63 -0.4247121891678809 2316 64-65 -0.16619411066256973 2316 66-67 -0.1542659447159629 2316 68-69 2.3582873023165796 2316 70-71 3.428146615918493 2316 72-73 2.0780293596994284 2316 74-75 0.9017700902251953 2316 76-77 -1.755482927042614 2316 78-79 -0.22713225597716402 2316 80-81 0.13776479799613384 2316 82-83 0.37811712552094434 2316 84-85 0.33098965660833457 2316 86-87 0.41385453923330573 2316 88-89 0.16523969566043561 2316 90-91 0.20550498032217135 2316 92-93 0.05825960965933419 2316 94-95 0.465354729873642 2316 96-97 0.5264791632216017 2316 98-99 0.6059321823688286 2316 100-101 0.5899953173270838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 33.0 4 74.0 5 175.0 6 534.0 7 1272.0 8 2716.0 9 4521.0 10 6881.0 11 9906.0 12 13007.0 13 16092.0 14 19548.0 15 24296.0 16 28564.0 17 31933.0 18 29862.0 19 27829.0 20 27474.0 21 28019.0 22 29631.0 23 32608.0 24 38542.0 25 48196.0 26 59351.0 27 73705.0 28 89341.0 29 105368.0 30 123250.0 31 142721.0 32 163503.0 33 188120.0 34 215166.0 35 238363.0 36 236074.0 37 183916.0 38 70178.0 39 3592.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.23043346730628 20.6352478499503 15.307921690652146 22.826396992091276 2 16.339836798265818 21.593546262531625 37.9664866855147 24.10013025368786 3 19.199664009768604 25.060049180051617 30.669824352637647 25.07046245754213 4 13.26449374229436 17.79968163346853 36.46991972510291 32.4659048991342 5 13.35117840098688 38.06802297822513 34.16107658040111 14.419722040386887 6 31.641119702380564 36.90416161668536 16.860607553259747 14.594111127674331 7 27.76273405448147 33.40311489328606 20.59510924162783 18.239041810604636 8 26.552337250174453 35.10954408660691 19.423686410743336 18.9144322524753 9 26.084303901934224 16.34219321498554 20.403566421026934 37.16993646205331 10-11 24.967611430507397 27.231505457009415 26.78452591258414 21.01635719989905 12-13 25.687888470487586 24.569439133412406 27.734367742339693 22.00830465376032 14-15 22.701929038850828 25.851864334277437 26.243137966569662 25.20306866030207 16-17 21.841755671081078 29.101240671522888 26.89429414676206 22.16270951063397 18-19 21.557110290343264 27.027814120855666 29.96933498792757 21.4457406008735 20-21 22.432265437819876 26.18253819082124 30.13923905693354 21.24595731442534 22-23 22.169925558269767 26.202724251472493 29.970330897023175 21.657019293234566 24-25 21.884841729700906 26.472912038256318 30.080441708873735 21.56180452316904 26-27 21.80089544183679 26.644007317189626 29.94065315877338 21.614444082200205 28-29 21.72701125399602 26.620774404380356 30.07624009244994 21.575974249173683 30-31 21.74674921209387 26.52804531011138 29.989690463289666 21.735515014505076 32-33 21.6611755759164 26.655180385031684 29.81977700050338 21.863867038548538 34-35 22.049890928543018 26.624886008066774 29.746855270159532 21.578367793230676 36-37 21.964452100159647 26.514792596166092 29.696986340588555 21.823768963085705 38-39 21.831981137522607 26.762923891599144 29.815231022313665 21.589863948564584 40-41 22.007892479614195 26.513927084835615 29.844673700606865 21.633506734943325 42-43 21.738898225117172 26.70843607749252 29.52590363686214 22.026762060528167 44-45 21.92340342354116 26.83278116555044 29.375722463361676 21.868092947546728 46-47 22.02763151521861 27.02676230046981 28.915985421409147 22.029620762902432 48-49 22.160856834170104 27.156490460029243 28.66819210489503 22.01446060090562 50-51 22.06413942228381 27.352884008126882 28.51576697196173 22.067209597627578 52-53 22.283555035781877 27.28573296915549 28.341625332769844 22.089086662292786 54-55 22.043485591263977 27.288062321133676 28.47981998806837 22.188632099533976 56-57 22.11476975361161 27.596956283365344 28.269433451276527 22.018840511746525 58-59 22.137049600602975 27.294744762330353 28.303166609259062 22.26503902780761 60-61 22.147889119460274 27.263318378363895 28.545913715213704 22.042878786962124 62-63 22.136540392250566 27.238856999155043 28.53881165089488 22.085790957699516 64-65 22.047571751822815 27.152835557887045 28.436772902366876 22.362819787923264 66-67 21.73437537869214 27.511637750456163 28.478536162286243 22.275450708565455 68-69 22.027750111439236 26.776062851730092 29.144973742129604 22.051213294701064 70-71 21.523454004842673 26.725622350776874 29.54454183183546 22.206381812544997 72-73 21.99357162790728 27.15990461740821 28.531034795304073 22.315488959380435 74-75 21.947318601690764 27.38905712198837 28.150159083495762 22.513465192825105 76-77 21.32108013511106 27.56673018984866 28.669318865952086 22.442870809088195 78-79 21.8240122452719 27.514005832234247 28.111360410987558 22.550621511506304 80-81 21.735972359533474 27.63031221697559 28.00386123075434 22.6298541927366 82-83 21.91034782541064 27.683766563166234 27.76340111704421 22.642484494378916 84-85 21.78506569626286 27.577501887128335 27.790368235233625 22.847064181375178 86-87 21.82959180873687 27.549242876793805 27.898049513042604 22.723115801426726 88-89 21.786381858609356 27.460924301122475 27.776865596187967 22.975828244080205 90-91 21.63732329702951 27.669800130927097 27.887795909284375 22.80508066275902 92-93 21.763537679166113 27.573616308950193 27.81107851614099 22.851767495742706 94-95 21.534847416876033 27.351254697276982 28.005868082732615 23.108029803114366 96-97 21.647683347824497 27.182595386125264 27.88610311269372 23.283618153356514 98-99 21.470310518207373 27.21590209309712 27.729118185334272 23.584669203361226 100-101 21.34167447757653 27.2020175699017 27.95411203891324 23.502195913608528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3281.0 1 2261.0 2 1299.0 3 1404.5 4 1309.5 5 1246.0 6 1860.5 7 3315.0 8 4795.0 9 5565.0 10 5158.5 11 4130.5 12 3616.5 13 3503.5 14 3517.0 15 3605.5 16 3727.5 17 3947.5 18 4257.5 19 4639.5 20 5186.5 21 5950.0 22 6932.0 23 8251.0 24 9695.5 25 11665.5 26 14736.5 27 17956.5 28 20704.5 29 23945.0 30 27666.0 31 31880.0 32 36543.5 33 40758.5 34 45272.0 35 50260.0 36 56168.5 37 61582.0 38 64381.5 39 65388.5 40 66184.0 41 68476.0 42 71221.0 43 73603.5 44 76475.5 45 79323.5 46 80392.5 47 81323.0 48 84473.5 49 85897.0 50 84360.0 51 82454.5 52 79999.5 53 78663.5 54 77442.5 55 72521.0 56 66010.5 57 59929.0 58 54253.0 59 47706.5 60 40092.0 61 32531.0 62 25781.5 63 20052.0 64 14860.0 65 10964.0 66 8227.0 67 6151.0 68 4389.5 69 2998.5 70 2109.5 71 1506.0 72 1117.5 73 931.5 74 732.5 75 537.0 76 375.5 77 222.0 78 127.5 79 80.5 80 55.0 81 35.0 82 21.0 83 10.0 84 5.5 85 8.0 86 6.5 87 2.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02009190425883558 2 0.0181043180310798 3 5.185007550667246E-4 4 0.0836514551507649 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011968725762790226 12-13 0.0 14-15 0.0 16-17 5.833133494500651E-4 18-19 4.3208396255560384E-5 20-21 0.0 22-23 1.9443778315002172E-4 24-25 0.0034782758985726107 26-27 0.0038239430686170936 28-29 0.001361064482050152 30-31 1.2962518876668115E-4 32-33 4.3208396255560384E-4 34-35 0.0024412743884391617 36-37 0.08972223482467113 38-39 0.09771578813194981 40-41 0.08254964104624811 42-43 0.08585508335979847 44-45 0.0848612902459206 46-47 0.08352182996199821 48-49 0.07801275943941427 50-51 0.07749425868434755 52-53 0.060167691785867825 54-55 0.05120194956283905 56-57 0.029403313651908836 58-59 0.02169061492029131 60-61 0.013264977650457036 62-63 0.0023116491996724806 64-65 0.006718905617739639 66-67 0.1633709462422738 68-69 0.740980787386605 70-71 0.9075491549517903 72-73 0.36699051359660206 74-75 0.054939475838945026 76-77 0.2034899421655616 78-79 0.014215562368079365 80-81 0.0033270465116781492 82-83 0.0018579610389890964 84-85 8.641679251112077E-5 86-87 0.0019659820296279973 88-89 4.752923588111642E-4 90-91 0.0043208396255560375 92-93 0.004407256418067159 94-95 0.0801515750540645 96-97 0.038304243280554276 98-99 0.014258770764334926 100-101 0.012703268499134751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2314365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54710812408877 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3729543804055 39.824566297154945 2 14.120352031650807 15.122080339780045 3 5.012048057024375 8.051420376978397 4 2.26207527570511 4.845103574920378 5 1.1624354905000949 3.1122529448543372 6 0.7099657454514771 2.280996752165372 7 0.4584285889053394 1.7183267652101355 8 0.3265497341716279 1.3988635138864485 9 0.22950855527225011 1.1060567482109922 >10 1.2008429384189747 11.918114223325638 >50 0.09563047688234227 3.5798108774881134 >100 0.046758748136935986 4.556721917835677 >500 0.0016333183167752251 0.5176288880469722 >1k 5.716614108711712E-4 0.614359064642235 >5k 8.166591583873875E-5 0.3309466439755452 >10k+ 1.633318316774775E-4 1.022751071524848 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12112 0.5233400954473474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11276 0.48721787617769885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7568 0.327001142862081 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3804 0.1643647393561517 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2930 0.12660060102879192 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.11126162035806797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.320839625556038E-5 14-15 0.0 0.0 0.0 4.320839625556038E-5 4.320839625556038E-5 16-17 0.0 0.0 0.0 4.320839625556038E-5 4.320839625556038E-5 18-19 0.0 0.0 0.0 8.641679251112076E-5 4.320839625556038E-5 20-21 0.0 0.0 0.0 8.641679251112076E-5 4.320839625556038E-5 22-23 0.0 0.0 0.0 3.2406297191670284E-4 4.320839625556038E-5 24-25 0.0 0.0 0.0 0.0010802099063890094 6.481259438334056E-5 26-27 0.0 0.0 0.0 0.0016203148595835143 8.641679251112076E-5 28-29 0.0 0.0 0.0 0.0033270465116781492 8.641679251112076E-5 30-31 0.0 0.0 0.0 0.007302218967189704 8.641679251112076E-5 32-33 0.0 0.0 0.0 0.01477727151940165 8.641679251112076E-5 34-35 0.0 0.0 0.0 0.02832310374551983 8.641679251112076E-5 36-37 0.0 0.0 0.0 0.05096430338343347 8.641679251112076E-5 38-39 0.0 0.0 0.0 0.08358664255638155 8.641679251112076E-5 40-41 0.0 0.0 0.0 0.1180021301739354 8.641679251112076E-5 42-43 0.0 0.0 0.0 0.15438359982111724 8.641679251112076E-5 44-45 0.0 0.0 0.0 0.2001196872576279 8.641679251112076E-5 46-47 0.0 0.0 0.0 0.2571979787112232 8.641679251112076E-5 48-49 0.0 0.0 0.0 0.3170416075251743 1.2962518876668115E-4 50-51 0.0 0.0 0.0 0.3794129275200757 1.2962518876668115E-4 52-53 0.0 0.0 0.0 0.44277804062885495 1.2962518876668115E-4 54-55 0.0 0.0 0.0 0.5162539184614354 1.2962518876668115E-4 56-57 0.0 0.0 0.0 0.6031460033313674 1.2962518876668115E-4 58-59 0.0 0.0 0.0 0.6903837553713437 1.2962518876668115E-4 60-61 0.0 0.0 0.0 0.7787017173177091 1.2962518876668115E-4 62-63 0.0 0.0 0.0 0.8684887647367636 1.2962518876668115E-4 64-65 0.0 0.0 0.0 0.96555642692488 1.2962518876668115E-4 66-67 0.0 0.0 0.0 1.069947912278314 1.2962518876668115E-4 68-69 0.0 0.0 0.0 1.1875611668859491 1.728335850222415E-4 70-71 0.0 0.0 0.0 1.338660928591644 1.728335850222415E-4 72-73 0.0 0.0 0.0 1.5030040637496678 1.728335850222415E-4 74-75 0.0 0.0 0.0 1.6767450250932763 1.728335850222415E-4 76-77 0.0 0.0 0.0 1.8804294050419879 1.728335850222415E-4 78-79 0.0 0.0 0.0 2.1156991226535142 1.728335850222415E-4 80-81 0.0 0.0 0.0 2.3762025436782874 1.728335850222415E-4 82-83 0.0 0.0 0.0 2.6462118118792843 1.728335850222415E-4 84-85 0.0 0.0 0.0 2.917560540364204 1.728335850222415E-4 86-87 0.0 0.0 0.0 3.200165056073696 1.9443778315002172E-4 88-89 0.0 0.0 0.0 3.515867203314948 2.376461794055821E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6415 0.0 50.595688 1 GTATCAA 10650 0.0 36.179325 1 ATCAACG 13805 0.0 27.765589 3 TATCAAC 13900 0.0 27.54527 2 TCAACGC 13955 0.0 27.406267 4 TGGTATC 3320 0.0 27.29503 2 GTGGTAT 3425 0.0 26.877892 1 CAACGCA 14210 0.0 26.822723 5 AACGCAG 14610 0.0 26.185703 6 GTACATG 11015 0.0 23.521347 1 TACATGG 11280 0.0 22.502615 2 ACGCAGA 17105 0.0 22.199865 7 CGCAGAG 17190 0.0 22.090094 8 GAGTACT 10570 0.0 21.007399 12-13 ACATGGG 11455 0.0 20.830748 3 GCAGAGT 18315 0.0 19.9308 9 CATGGGA 6790 0.0 19.846924 4 AGCGTAC 120 0.0047929096 19.771643 1 AGTACTT 11240 0.0 19.649763 12-13 ATAACGC 245 1.7820275E-7 19.362696 3 >>END_MODULE