FastQCFastQC Report
Fri 27 May 2016
SRR939401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814357
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104390.5753553462741897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80130.4416440645363619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.30914533358098767No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38470.212031039095393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.10350774406580404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85750.036.120871
GTATCAA134000.033.9833871
GTACATG105900.028.3060461
TATCAAC165150.027.3181572
TACATGG108950.027.278572
ATCAACG166800.026.6660443
TCAACGC169300.026.3386524
CAACGCA169350.026.237745
AACGCAG173250.025.8445746
ACATGGG112600.024.8320983
GAGTACT94200.024.17364112-13
ACGCAGA188700.023.3288967
CGCAGAG190500.023.150358
GCAGAGT187800.022.5483179
GTACTTT101750.022.5419314-15
TTAACGC2354.280082E-922.395434
CATGGGG36000.022.3275644
CATGGGA74800.022.0674464
AGTACTT98700.021.9555312-13
AGAGTAC154950.020.93150110-11
Produced by FastQC (version 0.11.5)