FastQCFastQC Report
Fri 27 May 2016
SRR939401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814357
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139750.7702453265812627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122300.6740680031548367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90440.49846860347770594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58720.3236408270257728No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.16468644263504922No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.14930909407575244No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23940.1319475715088045No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.10758632397042038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91200.035.599671
GTATCAA141650.032.490241
GTACATG118950.025.7405761
ATCAACG179650.025.4191273
TATCAAC181300.025.2710482
TCAACGC182050.025.0584
TACATGG121150.024.6774642
CAACGCA184350.024.616895
AACGCAG188150.024.1952426
ACATGGG123950.023.1216953
GAGTACT109750.021.86239212-13
CATGGGG37950.021.5945974
AGTACTT114550.021.56661412-13
ACGCAGA214600.021.1910427
CGCAGAG215000.021.0855188
GTACTTT116950.020.41517314-15
AGAGTAC182750.020.3097410-11
CATGGGA81550.020.2146424
GCAGAGT219700.019.8150989
TATGACG1456.2617066E-419.6017134
Produced by FastQC (version 0.11.5)