##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939401_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1814357 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.934603278186156 31.0 31.0 31.0 28.0 34.0 2 31.188891712050054 31.0 31.0 34.0 28.0 34.0 3 31.164743212058045 31.0 31.0 34.0 28.0 34.0 4 35.11630346177737 35.0 35.0 37.0 32.0 37.0 5 34.74272758889237 35.0 35.0 37.0 32.0 37.0 6 34.7131435544383 35.0 35.0 37.0 31.0 37.0 7 34.58781375440445 35.0 35.0 37.0 30.0 37.0 8 34.6066049845758 35.0 35.0 37.0 30.0 37.0 9 35.92304711806993 37.0 35.0 39.0 30.0 39.0 10-11 35.78757543305976 37.0 35.0 39.0 30.0 39.0 12-13 35.74193915530405 37.0 35.0 39.0 30.0 39.0 14-15 36.549564115551675 38.0 35.0 40.0 30.0 41.0 16-17 36.573434831182624 38.0 35.0 40.0 30.5 41.0 18-19 36.584060909732756 38.0 35.0 40.0 30.0 41.0 20-21 36.50339183523419 38.0 35.0 40.0 30.0 41.0 22-23 36.356229231623104 38.0 34.5 40.0 30.0 41.0 24-25 36.12962608791985 38.0 34.0 40.0 29.0 41.0 26-27 35.73859968021729 38.0 34.0 40.0 27.5 41.0 28-29 35.459881103884186 38.0 34.0 40.0 27.0 41.0 30-31 35.210390237422956 37.5 34.0 40.0 27.0 41.0 32-33 34.95192980212825 37.0 33.0 40.0 27.0 41.0 34-35 34.86334828261472 37.0 33.0 40.0 26.0 41.0 36-37 34.81322777160173 38.0 33.0 40.0 25.0 41.0 38-39 34.76140913833386 38.0 33.0 40.0 25.0 41.0 40-41 34.52847289700979 37.5 33.0 40.0 24.5 41.0 42-43 34.19497292980378 37.0 33.0 40.0 24.0 41.0 44-45 33.9611724704675 37.0 33.0 40.0 24.0 41.0 46-47 33.561026854141716 37.0 32.5 40.0 22.5 41.0 48-49 33.19934913580955 37.0 31.5 40.0 20.5 41.0 50-51 32.936685834155014 37.0 31.0 40.0 19.0 41.0 52-53 32.58450293960891 36.0 31.0 39.0 18.0 41.0 54-55 32.35855016405261 36.0 31.0 39.0 16.0 40.0 56-57 31.970317308004983 36.0 30.0 39.0 14.0 40.0 58-59 31.55101118467865 35.0 29.5 39.0 11.0 40.0 60-61 31.269390753859355 35.0 29.0 39.0 8.5 40.0 62-63 30.90329549256293 35.0 29.0 38.0 8.0 40.0 64-65 30.472852365879483 34.5 28.0 38.0 7.5 40.0 66-67 28.735499408330334 33.5 25.0 37.5 4.0 39.5 68-69 24.99633864779644 29.5 13.5 35.0 2.0 39.0 70-71 24.377109356096952 28.5 11.0 35.0 2.0 38.0 72-73 26.26081719308824 31.0 21.0 35.0 2.0 38.0 74-75 26.885644060127085 31.0 22.5 35.0 2.0 38.0 76-77 26.972072750842308 31.5 25.0 34.5 2.0 36.5 78-79 27.8187603101264 32.5 26.0 35.0 2.0 37.0 80-81 27.65901694098791 33.0 26.0 35.0 2.0 37.0 82-83 27.239249497204796 32.5 26.0 35.0 2.0 36.0 84-85 26.88588243658773 32.0 25.0 35.0 2.0 36.0 86-87 26.431434386948105 32.0 24.5 34.5 2.0 35.5 88-89 25.921998261643104 32.0 23.5 34.0 2.0 35.0 90-91 25.453513558797965 31.0 21.0 34.0 2.0 35.0 92-93 24.863630200671643 31.0 19.0 34.0 2.0 35.0 94-95 23.451343092897375 30.5 7.0 34.0 2.0 35.0 96-97 22.642822498549073 30.0 2.0 34.0 2.0 35.0 98-99 22.295415951766934 30.0 2.0 34.0 2.0 35.0 100-101 21.04774694285634 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3625804740272933 1101 2 0.19339446728911014 1101 3 0.34069348098198304 1101 4 0.2539974939524612 1101 5 0.3636980591392813 1101 6 0.5772411009979805 1101 7 0.5107970347539492 1101 8 0.5009396922659732 1101 9 0.6825376861575378 1101 10-11 0.737688251561579 1101 12-13 0.6582563934212047 1101 14-15 0.7982182870228982 1101 16-17 0.7083692021717738 1101 18-19 0.8825787714111542 1101 20-21 0.8091753523787446 1101 22-23 0.8705469440888436 1101 24-25 0.7565245414857031 1101 26-27 0.9012809224510576 1101 28-29 0.8446805653975957 1101 30-31 0.7838620576413007 1101 32-33 0.8119805043376758 1101 34-35 0.8703769294382226 1101 36-37 0.8755500233341778 1101 38-39 0.9786719733851825 1101 40-41 1.1941413187149692 1101 42-43 1.015771458285748 1101 44-45 0.8999550539562264 1101 46-47 0.8152130664705552 1101 48-49 0.8507392281825403 1101 50-51 0.7389471993035954 1101 52-53 0.8240981042809423 1101 54-55 0.6914900983764127 1101 56-57 0.5971634304450539 1101 58-59 0.6445008200630191 1101 60-61 0.3968650539522294 1101 62-63 0.33959120190746006 1101 64-65 0.05913448301893709 1101 66-67 -2.7904006042700154 1101 68-69 -7.868498805560673 1101 70-71 -10.983776515044251 1101 72-73 -11.15673561226519 1101 74-75 -9.731793743223026 1101 76-77 -4.652784736689913 1101 78-79 -1.536066725197628 1101 80-81 -0.936096198863968 1101 82-83 -0.6005813733028127 1101 84-85 -0.6710022039163839 1101 86-87 -0.6575749015241144 1101 88-89 -0.6822455740611097 1101 90-91 -0.6860085014250359 1101 92-93 -1.3858854880367808 1101 94-95 -4.912967153607358 1101 96-97 -1.7501040439840203 1101 98-99 -1.1029808774256793 1101 100-101 -1.0270783779018124 1102 1 0.4365900174128292 1102 2 0.2459452741016257 1102 3 0.3799603926184929 1102 4 0.26088219044414984 1102 5 0.4477471463878544 1102 6 0.37833180535846367 1102 7 0.4609391194850829 1102 8 0.5075465489786026 1102 9 0.7386444423725607 1102 10-11 0.7264131979536046 1102 12-13 0.7611439504855184 1102 14-15 0.7742447784128714 1102 16-17 0.7705156976926943 1102 18-19 0.7573221138614556 1102 20-21 0.8997275783728753 1102 22-23 0.9320505880985763 1102 24-25 0.6765695377600665 1102 26-27 0.6417934491162285 1102 28-29 0.6709940579502245 1102 30-31 0.5679555637194582 1102 32-33 0.4232229101059062 1102 34-35 0.5221339717660314 1102 36-37 0.7560770305378028 1102 38-39 0.540477665436093 1102 40-41 0.5381127396400132 1102 42-43 0.771293662703421 1102 44-45 0.5514096040545553 1102 46-47 0.5483102026775626 1102 48-49 0.33864620182774274 1102 50-51 0.5124845705306385 1102 52-53 0.45855723285013283 1102 54-55 0.3894859207854964 1102 56-57 0.3001738055731771 1102 58-59 0.2671536458223862 1102 60-61 0.2339996396744617 1102 62-63 0.08982900921635917 1102 64-65 0.22563324650976924 1102 66-67 -2.4572147414509082 1102 68-69 -7.7545270848085295 1102 70-71 -11.082198758217356 1102 72-73 -9.07779491251316 1102 74-75 -7.558864021584721 1102 76-77 -3.976087187831979 1102 78-79 -1.48796124380301 1102 80-81 -0.643864385876121 1102 82-83 -0.34292173480545785 1102 84-85 -0.5093214398236761 1102 86-87 -0.4081921566847484 1102 88-89 -0.28499954149544493 1102 90-91 -0.4528419607968033 1102 92-93 -0.9311094089090766 1102 94-95 -4.504089717373365 1102 96-97 -1.3232837325858142 1102 98-99 -0.5016800720012498 1102 100-101 -0.25240721604959404 1103 1 0.41281710643328395 1103 2 0.29754150907875854 1103 3 0.3354218029067191 1103 4 0.24947788477534516 1103 5 0.44239076322055837 1103 6 0.3010876815594088 1103 7 0.5158556738864775 1103 8 0.4737566209465527 1103 9 0.7279376585954864 1103 10-11 0.7712246587612057 1103 12-13 0.44087969870404464 1103 14-15 0.6894238563013317 1103 16-17 0.6279451478291804 1103 18-19 0.7210517510082539 1103 20-21 0.7557833668296325 1103 22-23 0.7809902351719984 1103 24-25 0.9051818946218404 1103 26-27 0.8210036185777128 1103 28-29 0.792003470507396 1103 30-31 0.9157337083289505 1103 32-33 0.9455224501137067 1103 34-35 0.9420030423868013 1103 36-37 0.8843427314276582 1103 38-39 0.881142549152905 1103 40-41 0.8520471131997667 1103 42-43 0.9867935829359951 1103 44-45 1.037979648724317 1103 46-47 0.7969631301423732 1103 48-49 0.5901800631033112 1103 50-51 0.6329471330151293 1103 52-53 0.860325300173848 1103 54-55 0.9046306543622649 1103 56-57 0.8035371107811038 1103 58-59 0.7845781769671341 1103 60-61 0.7883427548586361 1103 62-63 0.7675398832901692 1103 64-65 0.6092483509164737 1103 66-67 -2.529395627401204 1103 68-69 -7.369322614287029 1103 70-71 -11.204962943525967 1103 72-73 -8.970029220486357 1103 74-75 -7.398052321474822 1103 76-77 -3.6615265291302386 1103 78-79 -0.9760608290123578 1103 80-81 -0.4967475354489217 1103 82-83 -0.20158647228197069 1103 84-85 -0.1754289220089511 1103 86-87 -0.18306674455300964 1103 88-89 -0.03507720395512237 1103 90-91 -0.05197615177776527 1103 92-93 -0.10784579083554746 1103 94-95 0.8190903664710696 1103 96-97 1.092187936680677 1103 98-99 0.9887430720764598 1103 100-101 1.2506609496539554 1104 1 0.3569001607434821 1104 2 0.1753151882116093 1104 3 0.42392415805115036 1104 4 0.1911791928736406 1104 5 0.39268089998918754 1104 6 0.49573469364725753 1104 7 0.5550365518162153 1104 8 0.3645155165824434 1104 9 0.7479546127978551 1104 10-11 0.8033658184570243 1104 12-13 0.6332513717078498 1104 14-15 0.7049610704208504 1104 16-17 0.7000537114748298 1104 18-19 0.7588608637146876 1104 20-21 0.8361748386530223 1104 22-23 0.6875940388721204 1104 24-25 0.7698089731077431 1104 26-27 0.9815099036920643 1104 28-29 1.0593088164014972 1104 30-31 0.9304258725080601 1104 32-33 0.6940159025284416 1104 34-35 0.7292340096985299 1104 36-37 0.6526598317624845 1104 38-39 0.5992620869203407 1104 40-41 0.7947353474089525 1104 42-43 0.7989938695258516 1104 44-45 0.6827625072453998 1104 46-47 0.6034236389103214 1104 48-49 0.5593760863142521 1104 50-51 0.6483336037035059 1104 52-53 0.5652113381399104 1104 54-55 0.5642023302035923 1104 56-57 0.6162907144358378 1104 58-59 0.6080079624503689 1104 60-61 0.503900541022869 1104 62-63 0.5434154366072335 1104 64-65 0.28721959261672225 1104 66-67 -2.7698030248247356 1104 68-69 -7.467422892364322 1104 70-71 -9.184790812262133 1104 72-73 -8.528688809896718 1104 74-75 -7.4215709569098145 1104 76-77 -3.7284991754045684 1104 78-79 -0.8172304561617807 1104 80-81 -0.08497677896518141 1104 82-83 0.32450632892113873 1104 84-85 0.2697944620214656 1104 86-87 0.3403250890546161 1104 88-89 0.26086141331972 1104 90-91 0.16020941515941445 1104 92-93 0.49205188097649355 1104 94-95 1.3770902316887081 1104 96-97 1.3375299646586605 1104 98-99 1.1947410645286496 1104 100-101 1.1251023122326949 1105 1 0.47488598755743183 1105 2 0.26890128436981087 1105 3 0.4044158839645995 1105 4 0.2969174340019691 1105 5 0.26827992236931664 1105 6 0.4877809809554634 1105 7 0.4370555210181237 1105 8 0.3943846237177624 1105 9 0.7095816899523228 1105 10-11 0.772438710224371 1105 12-13 0.7424374686334048 1105 14-15 0.875117957691451 1105 16-17 0.9730896950225727 1105 18-19 0.8747369231545932 1105 20-21 0.9822499711189892 1105 22-23 1.0229187579215164 1105 24-25 0.9511002907843178 1105 26-27 0.898554656275202 1105 28-29 1.0747543854583697 1105 30-31 0.9685187080277373 1105 32-33 0.9646255816557883 1105 34-35 0.9620093585607208 1105 36-37 0.9755302568429585 1105 38-39 0.8295483035544535 1105 40-41 0.8210985669552073 1105 42-43 0.7385448857056929 1105 44-45 0.8582110786081998 1105 46-47 0.760679723136036 1105 48-49 0.4678733794429064 1105 50-51 0.8225970795488493 1105 52-53 0.803274294480822 1105 54-55 0.7427699904768517 1105 56-57 0.8624982648995179 1105 58-59 1.1611346290658737 1105 60-61 1.0254699029225556 1105 62-63 1.0590715507600876 1105 64-65 0.9877693119538726 1105 66-67 1.461379087570858 1105 68-69 -5.563170792668583 1105 70-71 -5.699505948104488 1105 72-73 -2.007276997117831 1105 74-75 0.5031172061393789 1105 76-77 0.8974228586433064 1105 78-79 0.6897693289767091 1105 80-81 0.6990425604891435 1105 82-83 0.7549811248986238 1105 84-85 0.5585203716338363 1105 86-87 0.8240354212074905 1105 88-89 0.9624516282963391 1105 90-91 1.083632149425604 1105 92-93 1.051361091629019 1105 94-95 1.6817434701504546 1105 96-97 1.664253269075214 1105 98-99 1.3236068931075309 1105 100-101 1.1156280227144855 1106 1 0.2574798222658856 1106 2 0.22125382982795472 1106 3 0.16304105956460546 1106 4 0.11942045135421608 1106 5 0.20663937311227443 1106 6 0.2440273947581204 1106 7 0.26033365164474276 1106 8 0.34974067128310793 1106 9 0.30445763225659306 1106 10-11 0.3472663254278743 1106 12-13 0.35105126733149206 1106 14-15 0.47682325635986444 1106 16-17 0.2744954843817098 1106 18-19 0.26145908931671613 1106 20-21 0.3646919489191873 1106 22-23 0.46054192525100746 1106 24-25 0.5397681544989297 1106 26-27 0.5139122372490661 1106 28-29 0.5493893353010719 1106 30-31 0.621602343096292 1106 32-33 0.7132472452556442 1106 34-35 0.7069384755152726 1106 36-37 0.703706391070618 1106 38-39 0.6053587841543688 1106 40-41 0.6546361898752124 1106 42-43 0.6562349788973592 1106 44-45 0.5025483493388876 1106 46-47 0.5124344865954029 1106 48-49 0.41684179122739806 1106 50-51 0.44592269637480086 1106 52-53 0.7637118600216652 1106 54-55 0.7812585443242241 1106 56-57 0.6799034408952416 1106 58-59 0.8983969507453047 1106 60-61 0.9308855555610016 1106 62-63 0.8415347984204011 1106 64-65 0.8768826061110886 1106 66-67 1.6599060260960137 1106 68-69 -5.259247987421357 1106 70-71 -5.86822293035652 1106 72-73 -2.135515209658145 1106 74-75 0.3954004514253846 1106 76-77 0.8107444688211807 1106 78-79 0.7329419512042072 1106 80-81 0.7242577323298285 1106 82-83 0.7555890471795443 1106 84-85 0.7631740191675185 1106 86-87 0.961522807685796 1106 88-89 0.8105613313097315 1106 90-91 0.7586316547776946 1106 92-93 0.7615562796941546 1106 94-95 1.459228828572332 1106 96-97 1.7202855003330058 1106 98-99 1.3772682769345224 1106 100-101 1.1239178270317716 1107 1 0.03122072495543904 1107 2 -0.014250547714123485 1107 3 0.05448220156635841 1107 4 0.11187054540489783 1107 5 0.19160972991396363 1107 6 0.1662711189680408 1107 7 0.2805442069869173 1107 8 0.16156397418538404 1107 9 0.25049790170439934 1107 10-11 0.32914938251811776 1107 12-13 0.35667264194864146 1107 14-15 0.36509708171120536 1107 16-17 0.1830068246285066 1107 18-19 0.40703501431253386 1107 20-21 0.32639461089809174 1107 22-23 0.2686301605451291 1107 24-25 0.4247051503922137 1107 26-27 0.5586844228686658 1107 28-29 0.4648788912439983 1107 30-31 0.5070205485502512 1107 32-33 0.4462384753991344 1107 34-35 0.5116802793186608 1107 36-37 0.5058331182054658 1107 38-39 0.31712065866810946 1107 40-41 0.4218255518078351 1107 42-43 0.418235303889233 1107 44-45 0.2996677395359555 1107 46-47 0.18205624036973944 1107 48-49 0.2838431749049235 1107 50-51 0.29887618314368325 1107 52-53 0.435768221491422 1107 54-55 0.5016301183682046 1107 56-57 0.4586556198180354 1107 58-59 0.31760717124736715 1107 60-61 0.3499466983506174 1107 62-63 0.3318813086970387 1107 64-65 0.32500774767952123 1107 66-67 1.066303441918194 1107 68-69 -5.525163356582585 1107 70-71 -6.109481387531453 1107 72-73 -2.2614245713937997 1107 74-75 0.35427777684073547 1107 76-77 0.19767991854099876 1107 78-79 0.3564906901372531 1107 80-81 0.33958405371724965 1107 82-83 0.44217402270666994 1107 84-85 0.362915502903153 1107 86-87 0.36948363139247675 1107 88-89 0.3995937172425066 1107 90-91 0.29906704540807993 1107 92-93 0.5311077416651813 1107 94-95 1.2554772651644441 1107 96-97 1.420377827570281 1107 98-99 0.932166298423379 1107 100-101 1.1805035904179881 1108 1 0.07635459271787326 1108 2 0.012773881957567568 1108 3 0.08563598877496048 1108 4 0.04740901838223266 1108 5 0.17419905559554394 1108 6 0.19195998169017514 1108 7 0.1833654625564165 1108 8 0.3260068877394744 1108 9 0.2054335815486965 1108 10-11 0.2853705550351222 1108 12-13 0.26581046406155195 1108 14-15 0.27400425867015343 1108 16-17 0.11014799311934809 1108 18-19 0.2634983642411868 1108 20-21 0.1569880214267414 1108 22-23 0.17144989871229654 1108 24-25 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1.0313969982926245 2312 82-83 1.1003267713290334 2312 84-85 1.1738410210109897 2312 86-87 1.208673804836792 2312 88-89 1.149121327957964 2312 90-91 1.2963935116572003 2312 92-93 1.4843315436458902 2312 94-95 2.1435823563082117 2312 96-97 2.059027749369374 2312 98-99 1.8660691819118966 2312 100-101 1.7360637965306829 2313 1 -0.31273098194754567 2313 2 -0.1334690336069997 2313 3 -0.08461939839394717 2313 4 -0.05356437180373774 2313 5 -0.12115189224366674 2313 6 -0.031120526385983283 2313 7 -3.805579479507582 2313 8 -1.233781045928815 2313 9 -1.0062464193234462 2313 10-11 -0.071277878451248 2313 12-13 -1.4323019657073246 2313 14-15 -0.4332124967776494 2313 16-17 -0.20716760033796788 2313 18-19 0.0928641411886133 2313 20-21 -0.034157848103816946 2313 22-23 -1.614996361782346 2313 24-25 -2.5773485771734457 2313 26-27 -1.9886770460962069 2313 28-29 -0.2996176693952606 2313 30-31 -0.10374192180858444 2313 32-33 -2.3773305758592684 2313 34-35 -2.6195934701452614 2313 36-37 -0.971457449837871 2313 38-39 -0.37164743782021503 2313 40-41 -0.2040407450394781 2313 42-43 -0.515015916589384 2313 44-45 -0.08573470407191763 2313 46-47 -0.03349900858886912 2313 48-49 -0.14029192152698755 2313 50-51 0.1160917112067068 2313 52-53 0.4541248452428235 2313 54-55 0.4059169327134029 2313 56-57 0.6529851092865506 2313 58-59 0.30317619667912865 2313 60-61 0.6625708616184802 2313 62-63 0.4357990399762919 2313 64-65 0.26704087510204033 2313 66-67 1.6162195393226888 2313 68-69 4.697196532199751 2313 70-71 4.709913263227815 2313 72-73 2.9859151148397203 2313 74-75 2.14452519604583 2313 76-77 1.4099660586749856 2313 78-79 1.2322152802922695 2313 80-81 1.1734577641693598 2313 82-83 1.0196906418092766 2313 84-85 1.15436640952014 2313 86-87 1.0278286263596783 2313 88-89 1.1142822445735483 2313 90-91 1.1752227025635236 2313 92-93 1.4503242225952668 2313 94-95 1.9158279025727012 2313 96-97 2.1416796736992403 2313 98-99 1.6629577353972032 2313 100-101 1.5500892563612858 2314 1 -0.17311341281084935 2314 2 -0.12765926090723312 2314 3 -0.1360622402919205 2314 4 -0.13870419540027257 2314 5 -1.5117913472911866 2314 6 -3.175408716715296 2314 7 -1.041861905070185 2314 8 -0.4620349036918938 2314 9 -0.14854984567914897 2314 10-11 0.09784836533918195 2314 12-13 -0.15915263684657788 2314 14-15 -2.738125550213759 2314 16-17 -3.063939801040661 2314 18-19 -0.5090324110528357 2314 20-21 -0.09853791065214068 2314 22-23 -0.03920428143565857 2314 24-25 -2.2113236458575827 2314 26-27 -1.2136447167964164 2314 28-29 -0.28893399846702295 2314 30-31 -0.017098148186697415 2314 32-33 0.06611940298791552 2314 34-35 -0.06002654774458449 2314 36-37 0.08754839793987657 2314 38-39 -1.3706960612871342 2314 40-41 -4.0580860830694405 2314 42-43 -5.425684285482447 2314 44-45 -2.268905065730017 2314 46-47 -3.1202635482726464 2314 48-49 -0.9199723690265031 2314 50-51 -0.8730090891997477 2314 52-53 -2.6264548718595577 2314 54-55 -1.5275867232728402 2314 56-57 -3.936557799920376 2314 58-59 -5.275398753789279 2314 60-61 -2.6836904411347895 2314 62-63 -1.294447332791286 2314 64-65 -0.3494521184661039 2314 66-67 -0.4137429456878188 2314 68-69 1.658665907626684 2314 70-71 1.6177505055810464 2314 72-73 -0.48868300671044906 2314 74-75 -0.03174999855505334 2314 76-77 -0.12188617767244736 2314 78-79 0.3332642198012259 2314 80-81 0.6761228686282514 2314 82-83 0.3233915340279445 2314 84-85 0.14804206838277167 2314 86-87 -0.09520298684487472 2314 88-89 0.0021915865160302417 2314 90-91 0.653064505523318 2314 92-93 0.7153637228487995 2314 94-95 0.599358062083919 2314 96-97 0.6329012561697063 2314 98-99 0.15692568313425426 2314 100-101 0.0850619077239152 2315 1 -0.3197400965386379 2315 2 -0.37478573137097726 2315 3 -0.4598901834171656 2315 4 -0.08224880665736123 2315 5 -1.336042236976695 2315 6 -0.3439530837769098 2315 7 -0.03900123649553677 2315 8 -0.046791245448346785 2315 9 0.04872428164762255 2315 10-11 -1.8924730284634634 2315 12-13 -1.6110200810316329 2315 14-15 -0.434331403742064 2315 16-17 -1.917112877024529 2315 18-19 -3.043643700697963 2315 20-21 -2.039933601741609 2315 22-23 -1.6961275487059737 2315 24-25 -1.3115372354984842 2315 26-27 -0.6427723528865528 2315 28-29 -0.941936137619372 2315 30-31 -3.214508048196784 2315 32-33 -2.8547485654034226 2315 34-35 -1.9890330628798196 2315 36-37 -5.105238067770188 2315 38-39 -2.667055269572849 2315 40-41 -2.2394663410049773 2315 42-43 -2.2711749718670866 2315 44-45 -2.126992982612393 2315 46-47 -1.757936723562672 2315 48-49 -5.177911815060931 2315 50-51 -4.616524396999999 2315 52-53 -2.932429195519493 2315 54-55 -4.61780425260072 2315 56-57 -6.782489666127308 2315 58-59 -7.811033385265752 2315 60-61 -6.667012215882373 2315 62-63 -7.185529639689644 2315 64-65 -6.732655702604468 2315 66-67 -4.748999562571168 2315 68-69 -2.197959441165853 2315 70-71 0.34020541895677425 2315 72-73 -1.362233055386742 2315 74-75 -3.3349258420876176 2315 76-77 -5.636067097214923 2315 78-79 -4.599811568705956 2315 80-81 -4.357486242793261 2315 82-83 -7.803197771512689 2315 84-85 -5.654200948440671 2315 86-87 -6.060283359715086 2315 88-89 -4.601558604770428 2315 90-91 -3.944655034347292 2315 92-93 -4.888895046210656 2315 94-95 -3.0177157598357205 2315 96-97 -2.199972338485342 2315 98-99 -3.3607381150495854 2315 100-101 -2.650956924631007 2316 1 -0.28452832930934946 2316 2 -0.42831656012577923 2316 3 -1.0130605046565861 2316 4 -0.2956214080861912 2316 5 -0.17976181657708423 2316 6 -2.239405079355123 2316 7 -0.4732420258172567 2316 8 -0.33886915466974443 2316 9 0.013341747700522433 2316 10-11 -0.06907262588298835 2316 12-13 -0.5447242338802241 2316 14-15 -1.013695029606204 2316 16-17 -1.3983454497447454 2316 18-19 -0.2213124462638092 2316 20-21 -3.8075926952755736 2316 22-23 -4.294572086645882 2316 24-25 -3.0535095498412304 2316 26-27 -2.25458577993534 2316 28-29 -1.3704112815748886 2316 30-31 -0.6632070862934327 2316 32-33 -1.997517791095973 2316 34-35 -2.373181044202724 2316 36-37 -4.73700191472361 2316 38-39 -2.0192859613842984 2316 40-41 -1.528344642907335 2316 42-43 -2.808630630267654 2316 44-45 -0.6891623798966222 2316 46-47 -0.49650195314902845 2316 48-49 -0.10839779899070834 2316 50-51 0.11589405570504852 2316 52-53 -2.087279547777406 2316 54-55 -0.5438956746667465 2316 56-57 0.5666020529243205 2316 58-59 0.48841677890128565 2316 60-61 -0.8683020408258635 2316 62-63 -0.46200673055073693 2316 64-65 0.7738973670716547 2316 66-67 1.852128982694385 2316 68-69 4.163738903964411 2316 70-71 4.535527895816646 2316 72-73 2.7401374671429117 2316 74-75 1.6584567413570888 2316 76-77 0.09218035778350853 2316 78-79 0.6497986376990319 2316 80-81 -0.08177883960231114 2316 82-83 0.30650334548313296 2316 84-85 0.5903716397888026 2316 86-87 -0.06937606871869662 2316 88-89 0.6711170704593279 2316 90-91 0.7760143381881406 2316 92-93 1.071338169978361 2316 94-95 1.4436719878585151 2316 96-97 1.8153339691587398 2316 98-99 0.6659827514883894 2316 100-101 0.6669834802593542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 28.0 4 65.0 5 194.0 6 405.0 7 1045.0 8 2047.0 9 3336.0 10 5446.0 11 8070.0 12 10391.0 13 12977.0 14 15893.0 15 19984.0 16 24202.0 17 26874.0 18 24301.0 19 20113.0 20 19071.0 21 19506.0 22 20721.0 23 23643.0 24 28395.0 25 34895.0 26 43816.0 27 54495.0 28 65756.0 29 78773.0 30 92003.0 31 107125.0 32 123403.0 33 142126.0 34 165411.0 35 188086.0 36 195579.0 37 164924.0 38 67725.0 39 3529.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.122379449725784 21.452513104129594 15.972594342015025 21.452513104129594 2 15.612181390100291 22.26544131712397 39.16840728263799 22.953970010137745 3 18.627756279497365 26.968286836603824 31.062574785447406 23.341382098451408 4 12.596142875961016 18.6359134393066 37.40537281251705 31.362570872215333 5 12.327748609146836 39.50623748177037 34.04236438974974 14.123649519333052 6 29.83211132098038 38.00084547859104 17.465416122626362 14.701627077802218 7 26.883573629666046 34.780035020671235 20.979608753955258 17.35678259570746 8 26.301383906254394 35.234135288700074 19.622543964611154 18.84193684043438 9 26.450196956828233 16.968545881543708 20.895281358630083 35.68597580299798 10-11 24.406029243417915 28.010060864537685 26.956244002696273 20.627665889348126 12-13 25.62334755508425 25.732063755920144 27.910686816321157 20.73390187267445 14-15 22.5763176706679 26.628166342125613 27.13958719259771 23.65592879460878 16-17 20.55031859912288 30.096717886139878 27.773239878325505 21.57972363641174 18-19 20.262988556428997 27.804611876235818 31.399091955052466 20.53330761228272 20-21 21.513522838538073 26.72391010256253 31.473362594396413 20.28920446450298 22-23 21.136496290421345 26.955417263526414 31.361992154796436 20.546094291255802 24-25 20.976307577920878 27.17886479310984 31.582492194891138 20.262335434078143 26-27 20.732637234446834 27.316925432503496 31.51771475120973 20.432722581839936 28-29 20.237843564908236 27.484917032308104 31.863628859080194 20.413610543703463 30-31 20.55311502698055 27.09533310918441 31.715621158923422 20.63593070491162 32-33 20.422722506018516 27.29985930379494 31.458844637490234 20.81857355269631 34-35 20.99614674504549 27.26925463039648 31.316894873470417 20.41770375108761 36-37 21.179326611401535 26.804590408143557 31.27375550007586 20.74232748037904 38-39 20.667776418022772 27.299800597875322 31.45546446790972 20.57695851619218 40-41 20.675851740177237 27.30993083879142 31.521243080706103 20.49297434032524 42-43 20.837058765596122 27.271626289463946 30.915850795851647 20.975464149088282 44-45 20.669345261494026 27.495431078596482 30.771895995486943 21.063327664422552 46-47 21.07607409892215 27.673578314103864 30.3014737687697 20.948873818204287 48-49 21.047000913407786 27.866358316824773 29.888573078278025 21.198067691489417 50-51 20.949914468470023 27.98719661548384 29.700558265303055 21.36233065074308 52-53 21.26984520977695 28.23561209363257 29.425118074947427 21.06942462164305 54-55 20.847674361206657 28.053491057293055 29.608926684887503 21.489907896612785 56-57 21.202901639195122 28.365554930363363 29.13637003977307 21.295173390668452 58-59 21.33399556192917 27.781380774184548 29.376820657783725 21.507803006102556 60-61 21.16927832556914 28.011809032553934 29.774399743799556 21.044512898077368 62-63 21.11456693607771 27.974434611602756 29.84162731325317 21.069371139066366 64-65 20.96385475761012 27.954819480481902 29.54636211935605 21.534963642551926 66-67 20.746449567626023 28.109221588444637 29.700373318895494 21.443955525033846 68-69 20.932730012064756 27.603865725165317 30.166007843621173 21.297396419148754 70-71 20.616062193750693 27.716456051155287 30.454262968112516 21.213218786981507 72-73 21.141363079823 27.976330314278368 29.298726137535137 21.583580468363493 74-75 20.638396179128733 28.418420070614577 29.242450003422533 21.700733746834157 76-77 20.75318547264614 28.397630774573546 29.15133992431488 21.697843828465434 78-79 20.85097528541998 28.24936439364187 29.035876447770036 21.86378387316811 80-81 20.806463880841832 28.57603476328426 28.80198618992817 21.81551516594574 82-83 20.974593202991475 28.502439156130794 28.87874326827631 21.64422437260142 84-85 20.681431493361007 28.440626624197996 28.696667745101983 22.181274137339013 86-87 20.836546140690373 28.218654253058805 28.89470792067475 22.05009168557607 88-89 20.825454006317422 28.192677585930365 28.665519881257556 22.316348526494657 90-91 20.66500707023356 28.28236729773772 28.895745541040284 22.156880090988434 92-93 20.936323601549883 28.284049759415318 28.671327707748866 22.10829893128593 94-95 20.335361204966777 27.86833713789453 29.042935559646168 22.753366097492524 96-97 20.3671735559016 27.850399027824622 29.09514926146219 22.68727815481158 98-99 20.60244347665269 28.00527866366462 28.468953260729656 22.92332459895303 100-101 20.314927861759152 27.75686587936998 28.919869365707118 23.008336893163747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4580.0 1 3039.5 2 1535.5 3 1573.0 4 1487.5 5 1492.5 6 2172.0 7 3869.5 8 5700.0 9 6590.5 10 5956.5 11 4773.0 12 4284.0 13 4216.0 14 4149.5 15 4150.0 16 4234.5 17 4387.0 18 4613.5 19 4810.0 20 5190.5 21 5857.0 22 6690.5 23 7558.0 24 8609.5 25 10351.5 26 12752.0 27 15193.0 28 17373.0 29 19873.5 30 23450.0 31 27824.0 32 32805.0 33 37841.5 34 42657.0 35 47354.5 36 53474.0 37 58577.0 38 59448.5 39 59379.0 40 59757.5 41 60755.0 42 61870.0 43 63094.0 44 64312.0 45 65284.5 46 64450.0 47 64109.0 48 67659.5 49 66428.5 50 59663.0 51 54113.5 52 50914.5 53 49387.0 54 47255.5 55 43651.0 56 39487.5 57 35450.5 58 31969.5 59 28277.5 60 24123.5 61 19897.0 62 15775.0 63 12093.5 64 9121.0 65 6935.5 66 5208.0 67 4032.5 68 2950.0 69 2054.0 70 1534.5 71 1205.0 72 1038.0 73 885.0 74 694.5 75 534.5 76 360.0 77 184.0 78 102.0 79 62.0 80 39.5 81 22.0 82 15.0 83 9.0 84 4.0 85 3.5 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0234242764792155 2 0.018959884962000313 3 0.0 4 0.0 5 1.6534783397093296E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.4092755725582116E-4 18-19 0.0010747609208110642 20-21 2.7557972328488823E-5 22-23 0.0 24-25 2.7557972328488823E-5 26-27 4.1336958492733234E-4 28-29 0.01694815298202063 30-31 0.004795087185157056 32-33 0.009066572896072824 34-35 0.009727964231956555 36-37 0.10372820784443194 38-39 0.10686981668987967 40-41 0.12202670147054852 42-43 0.10623598332632442 44-45 0.10138578019651039 46-47 0.1025707730066354 48-49 0.10546436010112673 50-51 0.10292902664690577 52-53 0.07823708344057977 54-55 0.06787528584506798 56-57 0.03816779167495702 58-59 0.015460022476282231 60-61 0.00840518156018909 62-63 0.0015983623950523518 64-65 0.004381717600229723 66-67 0.44051418767089384 68-69 0.8441282503939412 70-71 0.937301754836562 72-73 0.2916735791247257 74-75 0.15614347121321767 76-77 0.014440377500128145 78-79 0.00796425400293327 80-81 0.0015983623950523518 82-83 0.0 84-85 0.0 86-87 2.2046377862791058E-4 88-89 5.511594465697765E-4 90-91 0.0033345146517471482 92-93 0.0014881305057383965 94-95 0.23418764884749801 96-97 0.24226213473974526 98-99 0.014412819527799655 100-101 0.00727530469472105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1814357.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.93023560094754 #Duplication Level Percentage of deduplicated Percentage of total 1 72.22606122130784 31.72907885980514 2 13.872431153520909 12.188383378641959 3 5.37789714617109 7.08756866006878 4 2.637832849762574 4.635224742639549 5 1.5038118677390038 3.3031404824637693 6 0.9267531040616552 2.442748932320278 7 0.6500534510082314 1.9989900879200433 8 0.4325644745214987 1.5202127422663605 9 0.3121114523944432 1.234001667369763 >10 1.7531427686224672 14.441388376103134 >50 0.18762702398162776 5.741691886425099 >100 0.11361366481224867 9.165626362691263 >500 0.004193627691305279 1.1856964751768413 >1k 0.0013978758971017597 1.0306107927981387 >5k 2.5415925401850177E-4 0.8327062773076775 >10k+ 2.5415925401850177E-4 1.4629302760021246 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13975 0.7702453265812627 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12230 0.6740680031548367 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9044 0.49846860347770594 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5872 0.3236408270257728 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2988 0.16468644263504922 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.14930909407575244 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2394 0.1319475715088045 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1952 0.10758632397042038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.7557972328488826E-5 0.0 14-15 0.0 0.0 0.0 5.511594465697765E-5 0.0 16-17 0.0 0.0 0.0 1.3778986164244414E-4 0.0 18-19 0.0 0.0 0.0 1.6534783397093296E-4 0.0 20-21 0.0 0.0 0.0 4.4092755725582127E-4 0.0 22-23 0.0 0.0 0.0 9.64529031497109E-4 0.0 24-25 0.0 0.0 0.0 0.0017085942843663073 0.0 26-27 0.0 0.0 0.0 0.00234242764792155 0.0 28-29 0.0 0.0 0.0 0.004409275572558212 0.0 30-31 0.0 5.511594465697765E-5 0.0 0.011078304876052509 0.0 32-33 0.0 1.102318893139553E-4 0.0 0.02369985620250039 0.0 34-35 0.0 1.102318893139553E-4 0.0 0.039959059876308795 0.0 36-37 0.0 1.102318893139553E-4 0.0 0.06740680031548366 0.0 38-39 0.0 1.102318893139553E-4 0.0 0.10675958480056572 0.0 40-41 0.0 1.102318893139553E-4 0.0 0.15087989849847633 0.0 42-43 0.0 1.102318893139553E-4 0.0 0.19555137164295672 0.0 44-45 0.0 1.102318893139553E-4 0.0 0.24931697565583838 0.0 46-47 0.0 1.102318893139553E-4 0.0 0.3171922615009064 0.0 48-49 0.0 1.102318893139553E-4 0.0 0.3887051996933349 2.7557972328488826E-5 50-51 0.0 1.102318893139553E-4 0.0 0.470965747093874 5.511594465697765E-5 52-53 0.0 1.6534783397093296E-4 0.0 0.5510216567081341 5.511594465697765E-5 54-55 0.0 1.6534783397093296E-4 0.0 0.6386008927680715 5.511594465697765E-5 56-57 0.0 1.6534783397093296E-4 0.0 0.7365970423681778 5.511594465697765E-5 58-59 0.0 1.6534783397093296E-4 0.0 0.8304594961190108 5.511594465697765E-5 60-61 0.0 1.6534783397093296E-4 0.0 0.9263336818498233 5.511594465697765E-5 62-63 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.022373215414607 5.511594465697765E-5 64-65 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.125274684089184 5.511594465697765E-5 66-67 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.2371049357981918 5.511594465697765E-5 68-69 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.372304348041758 5.511594465697765E-5 70-71 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.5484273491931302 5.511594465697765E-5 72-73 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.7401206047100983 5.511594465697765E-5 74-75 5.511594465697765E-5 1.6534783397093296E-4 0.0 1.944573201415157 5.511594465697765E-5 76-77 5.511594465697765E-5 1.6534783397093296E-4 0.0 2.178760850262655 5.511594465697765E-5 78-79 5.511594465697765E-5 1.6534783397093296E-4 0.0 2.4425733193632784 5.511594465697765E-5 80-81 5.511594465697765E-5 1.6534783397093296E-4 0.0 2.7375538551674232 5.511594465697765E-5 82-83 5.511594465697765E-5 1.6534783397093296E-4 0.0 3.0376050578800093 5.511594465697765E-5 84-85 5.511594465697765E-5 1.6534783397093296E-4 0.0 3.3302982819808893 5.511594465697765E-5 86-87 5.511594465697765E-5 1.6534783397093296E-4 0.0 3.6272905497650134 5.511594465697765E-5 88-89 5.511594465697765E-5 1.6534783397093296E-4 0.0 3.957545290149623 5.511594465697765E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9120 0.0 35.59967 1 GTATCAA 14165 0.0 32.49024 1 GTACATG 11895 0.0 25.740576 1 ATCAACG 17965 0.0 25.419127 3 TATCAAC 18130 0.0 25.271048 2 TCAACGC 18205 0.0 25.058 4 TACATGG 12115 0.0 24.677464 2 CAACGCA 18435 0.0 24.61689 5 AACGCAG 18815 0.0 24.195242 6 ACATGGG 12395 0.0 23.121695 3 GAGTACT 10975 0.0 21.862392 12-13 CATGGGG 3795 0.0 21.594597 4 AGTACTT 11455 0.0 21.566614 12-13 ACGCAGA 21460 0.0 21.191042 7 CGCAGAG 21500 0.0 21.085518 8 GTACTTT 11695 0.0 20.415173 14-15 AGAGTAC 18275 0.0 20.30974 10-11 CATGGGA 8155 0.0 20.214642 4 GCAGAGT 21970 0.0 19.815098 9 TATGACG 145 6.2617066E-4 19.601713 4 >>END_MODULE