FastQCFastQC Report
Fri 27 May 2016
SRR939400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794517
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101700.5667263113138522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.4110855455813458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.3100555748427014No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38370.21381797999127342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA1301.1641532E-1038.5437741
GGTATCA80600.036.243581
GTATCAA128250.034.9673921
ACTCGAT650.00532592829.7921988
GTACATG107050.028.458661
TATCAAC159600.027.7779052
ATCAACG162500.027.2820013
CAACGCA164600.027.0552465
TCAACGC165200.027.0355054
TACATGG112050.026.6241592
AACGCAG167100.026.393556
ACATGGG114750.025.518983
ACGCAGA182900.023.8230367
CGCAGAG184550.023.4519738
GAGTACT85400.023.0801112-13
GCAGAGT179000.022.3953599
CATGGGG39250.021.9808184
AGTACTT89900.021.92481812-13
CATGGGA72100.021.8164274
GTACTTT94150.021.36733414-15
Produced by FastQC (version 0.11.5)