FastQCFastQC Report
Fri 27 May 2016
SRR939400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794517
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133570.7443228456459314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117140.6527661760796917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89510.4987971693776097No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58220.32443270250435075No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27590.1537461054980254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.15134991755441715No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22170.12354299234835892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.10593379722788918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89750.035.757781
GTATCAA135250.031.3102041
ATCAACG174000.024.0777193
GTACATG114400.024.0691221
TATCAAC175500.023.9306682
TCAACGC176150.023.7891454
CAACGCA178350.023.5006755
TACATGG115500.023.4197122
AACGCAG182900.022.890136
ACATGGG117700.021.969693
GAGTACT106850.021.67177212-13
ATAGCGT1553.963363E-521.4125086
AGTACTT112550.021.03751212-13
GTACTTT113350.020.55447614-15
AACGCAT1900.020.22222164-65
ACGCAGA207400.020.094697
AGAGTAC178150.019.99843210-11
CATGGGA77450.019.975474
CGCAGAG209450.019.920658
GTATAGG6200.019.9085661
Produced by FastQC (version 0.11.5)