##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939400_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1794517 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.827032566423167 31.0 31.0 31.0 28.0 34.0 2 31.07528933969419 31.0 31.0 34.0 28.0 34.0 3 31.0628770861463 31.0 31.0 34.0 27.0 34.0 4 34.99951073185709 35.0 35.0 37.0 32.0 37.0 5 34.56369151142062 35.0 35.0 37.0 30.0 37.0 6 34.632997625544924 35.0 35.0 37.0 30.0 37.0 7 34.47315740112799 35.0 35.0 37.0 30.0 37.0 8 34.381941213150945 35.0 35.0 37.0 30.0 37.0 9 35.85286291520225 37.0 35.0 39.0 30.0 39.0 10-11 35.64990886127019 37.0 35.0 39.0 30.0 39.0 12-13 35.62700994195095 37.0 35.0 39.0 30.0 39.0 14-15 36.392772818535576 38.0 35.0 40.0 30.0 41.0 16-17 36.32295570340097 38.0 34.5 40.0 30.0 41.0 18-19 36.37641047702529 38.0 35.0 40.0 30.0 41.0 20-21 36.28277776136977 38.0 34.0 40.0 29.5 41.0 22-23 36.11591447726603 38.0 34.0 40.0 29.0 41.0 24-25 35.93808528980222 38.0 34.0 40.0 29.0 41.0 26-27 35.450534043422266 38.0 34.0 40.0 27.0 41.0 28-29 35.23776620672861 38.0 33.5 40.0 27.0 41.0 30-31 34.90373147760651 37.0 33.0 40.0 26.5 41.0 32-33 34.617244640201235 37.0 33.0 40.0 25.0 41.0 34-35 34.79795956237807 37.0 33.0 40.0 26.0 41.0 36-37 34.61216611489331 37.0 33.0 40.0 25.0 41.0 38-39 34.40913460279284 37.0 33.0 40.0 25.0 41.0 40-41 34.23444079939058 37.0 33.0 40.0 24.5 41.0 42-43 34.03038254861893 37.0 33.0 40.0 24.0 41.0 44-45 33.60132754384606 37.0 32.0 40.0 23.0 41.0 46-47 33.11731151056246 37.0 31.0 40.0 20.0 41.0 48-49 32.67470912786003 36.5 31.0 39.0 18.5 40.5 50-51 32.42261009508408 36.0 31.0 39.0 17.0 40.0 52-53 32.31608700279797 36.0 31.0 39.0 16.5 40.0 54-55 31.97435911724436 36.0 30.0 39.0 14.0 40.0 56-57 31.4892411161332 35.0 29.5 39.0 11.0 40.0 58-59 31.035486707565322 35.0 28.5 38.5 8.5 40.0 60-61 30.67279078437262 35.0 28.0 38.0 8.0 40.0 62-63 30.510637124084084 34.0 28.0 38.0 7.5 40.0 64-65 30.057784908139627 34.0 28.0 38.0 6.0 40.0 66-67 29.168118496509088 33.5 27.0 37.5 2.0 39.5 68-69 25.692265383944537 31.0 15.5 36.0 2.0 39.0 70-71 24.351279480773936 29.0 11.5 35.0 2.0 38.0 72-73 25.396437035703755 30.0 16.5 35.0 2.0 38.0 74-75 26.15327912747553 31.0 21.0 35.0 2.0 37.0 76-77 25.840041080691908 30.5 21.0 34.0 2.0 36.0 78-79 26.972568941949284 32.0 25.0 35.0 2.0 37.0 80-81 26.968870732347476 32.0 25.0 35.0 2.0 36.5 82-83 26.626829113349164 32.0 24.5 35.0 2.0 36.0 84-85 26.247397489129387 32.0 24.0 34.5 2.0 36.0 86-87 25.792230165554294 31.5 22.5 34.0 2.0 35.0 88-89 25.285474308685846 31.0 20.5 34.0 2.0 35.0 90-91 24.678144035414544 31.0 19.0 34.0 2.0 35.0 92-93 24.085996956284056 31.0 15.5 34.0 2.0 35.0 94-95 23.209657250391054 30.0 4.5 34.0 2.0 35.0 96-97 22.534290285352547 30.0 2.0 34.0 2.0 35.0 98-99 21.66887078807278 29.0 2.0 34.0 2.0 35.0 100-101 20.261484009346248 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32880431393044063 1101 2 0.1894008300865515 1101 3 0.30047003881911394 1101 4 0.3406040658354357 1101 5 0.5362319909915527 1101 6 0.3833190146109118 1101 7 0.5471538140965748 1101 8 0.7960953774537813 1101 9 0.6352250570382196 1101 10-11 0.7836098713330486 1101 12-13 0.8459492579144694 1101 14-15 0.7644790965404695 1101 16-17 0.8268649048655803 1101 18-19 0.5235921106585195 1101 20-21 0.8294356313205498 1101 22-23 0.7385848150699488 1101 24-25 0.7072613391873332 1101 26-27 0.8050403177441652 1101 28-29 0.936498791071962 1101 30-31 0.5052558799684519 1101 32-33 0.7974644579807801 1101 34-35 0.5638439864544083 1101 36-37 0.8448568770093914 1101 38-39 0.9760034885443432 1101 40-41 1.028875139080732 1101 42-43 0.6812023649087706 1101 44-45 0.6844678963658382 1101 46-47 0.7463019403248268 1101 48-49 0.7507713888754566 1101 50-51 0.5228109084569397 1101 52-53 0.41708381671335104 1101 54-55 0.3598948516730758 1101 56-57 0.4483846528710451 1101 58-59 0.470856612000663 1101 60-61 0.5324819994435153 1101 62-63 0.12654229570223308 1101 64-65 0.1307036994205788 1101 66-67 -0.043782665987865954 1101 68-69 -6.788551986051743 1101 70-71 -7.581145112254045 1101 72-73 -4.667878049978366 1101 74-75 -2.760582836209096 1101 76-77 -0.23283189983072106 1101 78-79 0.037335948030726485 1101 80-81 -0.06441082681729071 1101 82-83 -0.19434694191340185 1101 84-85 -0.33640143384560517 1101 86-87 -0.2669974669693218 1101 88-89 -0.43442308354861936 1101 90-91 -0.5207076503646171 1101 92-93 -0.4994605177084459 1101 94-95 -0.48565407426938734 1101 96-97 -0.752222023713248 1101 98-99 -0.5681506463729633 1101 100-101 -0.673220242350304 1102 1 0.38321665775722025 1102 2 0.37384167066701934 1102 3 0.3271541674551166 1102 4 0.23486102234582518 1102 5 0.4205937306667309 1102 6 0.30053498051013605 1102 7 0.45477871011350146 1102 8 0.5495080084043664 1102 9 0.5569439866679247 1102 10-11 0.6472410454878244 1102 12-13 0.6033480297509115 1102 14-15 0.6831151336418557 1102 16-17 0.6255144016045122 1102 18-19 0.5079564605500835 1102 20-21 0.584581218976723 1102 22-23 0.6271595574813134 1102 24-25 0.4230660859225779 1102 26-27 0.6861546077709804 1102 28-29 0.5097721045600849 1102 30-31 0.5478667545838647 1102 32-33 0.8111865925022528 1102 34-35 0.6205094009737948 1102 36-37 0.6959003393819785 1102 38-39 0.9727631886191332 1102 40-41 0.8875725503135143 1102 42-43 0.5980531274891661 1102 44-45 0.872074475472985 1102 46-47 1.0768180935921947 1102 48-49 1.245920698927172 1102 50-51 1.0148674525531476 1102 52-53 0.7002617665132149 1102 54-55 0.5098639262832734 1102 56-57 0.7872903820690418 1102 58-59 0.9166499078301058 1102 60-61 0.9811094228308228 1102 62-63 0.4248365760372046 1102 64-65 0.35123509408189335 1102 66-67 0.3137243730848027 1102 68-69 -6.419475446563144 1102 70-71 -7.16273378073727 1102 72-73 -1.3842368435136514 1102 74-75 0.31452557288671557 1102 76-77 0.6518254752221928 1102 78-79 0.5620717203653847 1102 80-81 0.22924362365839457 1102 82-83 0.09417775243593596 1102 84-85 -0.1123962951239541 1102 86-87 0.02904351569770469 1102 88-89 0.07231399843373865 1102 90-91 -0.03638018396547693 1102 92-93 -0.11713383891373041 1102 94-95 0.053413147881588685 1102 96-97 -0.2523894180250217 1102 98-99 -0.18482209843633512 1102 100-101 -0.08996307815747784 1103 1 0.4429620295374299 1103 2 0.3133413018531108 1103 3 0.39584085158123017 1103 4 0.306469118657013 1103 5 0.48046113811925295 1103 6 0.5195019406489081 1103 7 0.4472470112912248 1103 8 0.6478734973478453 1103 9 0.6337150265426814 1103 10-11 0.6055973310016682 1103 12-13 0.6310559922965595 1103 14-15 0.9454689333274118 1103 16-17 0.7661274673165508 1103 18-19 0.7976950876600526 1103 20-21 0.6632659180493903 1103 22-23 0.6496463783054267 1103 24-25 0.7187561514067795 1103 26-27 0.6372337550100653 1103 28-29 0.648596140850465 1103 30-31 0.6979808951572295 1103 32-33 0.8278936369606811 1103 34-35 0.7613807492085627 1103 36-37 0.9928192206239004 1103 38-39 0.905938958334886 1103 40-41 0.8749994924514652 1103 42-43 0.7939208326997047 1103 44-45 0.8899662359221452 1103 46-47 0.8821073587389421 1103 48-49 1.0088538860883816 1103 50-51 0.8741731801090751 1103 52-53 0.8206792819030184 1103 54-55 0.5533660342746956 1103 56-57 0.8131027313572368 1103 58-59 1.052315678966977 1103 60-61 1.020757143515759 1103 62-63 0.8527342465318704 1103 64-65 0.8031214587948625 1103 66-67 0.7144944835936293 1103 68-69 -6.44725412663794 1103 70-71 -7.431975039170926 1103 72-73 -0.5149284767389339 1103 74-75 0.8983021596324221 1103 76-77 1.2355975504827903 1103 78-79 0.9568870088861345 1103 80-81 0.6515338565187001 1103 82-83 0.6826504568231293 1103 84-85 0.4025151782594101 1103 86-87 0.6055951846088341 1103 88-89 0.6799469800968048 1103 90-91 0.4431504185659172 1103 92-93 0.4047366002567898 1103 94-95 0.6475397581998088 1103 96-97 0.7232812706306255 1103 98-99 0.8130730681840852 1103 100-101 0.6450304816975247 1104 1 0.32909606365997135 1104 2 0.2842986650518533 1104 3 0.23322664586357433 1104 4 0.2848068728568336 1104 5 0.5162005689736233 1104 6 0.4406426879390821 1104 7 0.5872738639617552 1104 8 0.7455418996139471 1104 9 0.6946083690452198 1104 10-11 0.6426568817106428 1104 12-13 0.4781083386305198 1104 14-15 0.6844126737079321 1104 16-17 0.5916403696972665 1104 18-19 0.5777733533705032 1104 20-21 0.49355171888116445 1104 22-23 0.49877202915521934 1104 24-25 0.6046773289946259 1104 26-27 0.7507927162701762 1104 28-29 0.726640807975599 1104 30-31 0.6571165338905729 1104 32-33 0.7804655169750916 1104 34-35 0.6047162929751408 1104 36-37 0.7817289858267742 1104 38-39 0.8314514513761111 1104 40-41 0.6915192062412956 1104 42-43 0.8131127146789439 1104 44-45 0.9819591461621826 1104 46-47 0.9398610673547196 1104 48-49 0.8469580711753295 1104 50-51 0.7527201067881819 1104 52-53 0.6632943881675502 1104 54-55 0.6875638926104841 1104 56-57 0.7668719293793096 1104 58-59 0.8969631557983604 1104 60-61 0.8269103347264242 1104 62-63 0.6417463466071851 1104 64-65 0.5184115167573324 1104 66-67 0.4330387905000066 1104 68-69 -6.3538469484606495 1104 70-71 -4.118492624395412 1104 72-73 0.37654948442293446 1104 74-75 1.184559404941389 1104 76-77 1.3464098437663417 1104 78-79 0.8725712607772813 1104 80-81 0.7005870542787953 1104 82-83 0.5697525788390436 1104 84-85 0.7514156923898092 1104 86-87 0.6893869781707451 1104 88-89 0.6549404306649684 1104 90-91 0.6086607011738998 1104 92-93 0.7145206327419693 1104 94-95 0.99119049337353 1104 96-97 0.5819199712233463 1104 98-99 0.6743049344446881 1104 100-101 0.8012057772406322 1105 1 0.10308613173273073 1105 2 0.14356009420782456 1105 3 0.14781579142024626 1105 4 0.21675457214634974 1105 5 0.4028101363582195 1105 6 0.3318462366501649 1105 7 0.3299141942820327 1105 8 0.3844121893881578 1105 9 0.5571423124295194 1105 10-11 0.5909666871639061 1105 12-13 0.4720938499201779 1105 14-15 0.4861396015061672 1105 16-17 0.32362108241534315 1105 18-19 0.5191070958843582 1105 20-21 0.5809652329978974 1105 22-23 0.5174139218011291 1105 24-25 0.5249847723603196 1105 26-27 0.6374794699806969 1105 28-29 0.5814449592979898 1105 30-31 0.44285845257524414 1105 32-33 0.550105717101566 1105 34-35 0.5688697542703807 1105 36-37 0.627938021276691 1105 38-39 0.5382643070841837 1105 40-41 0.5028874501128797 1105 42-43 0.42505195137127316 1105 44-45 0.4368307192752994 1105 46-47 0.46819495866149197 1105 48-49 0.3841837342337655 1105 50-51 0.3328753164001981 1105 52-53 0.163626847342492 1105 54-55 0.15807034650327978 1105 56-57 0.4034377888444034 1105 58-59 0.40886100946053006 1105 60-61 0.4449858843302614 1105 62-63 0.19068015235803415 1105 64-65 0.08896772328190394 1105 66-67 0.4071790464238241 1105 68-69 -2.9065698588501157 1105 70-71 -3.1639502556121606 1105 72-73 0.63792144811746 1105 74-75 1.0870949292504335 1105 76-77 0.8532431585005646 1105 78-79 0.6008363353597339 1105 80-81 0.38096917308994094 1105 82-83 0.3650232889532461 1105 84-85 0.38121519393353864 1105 86-87 0.5779262037036084 1105 88-89 0.5531986235749891 1105 90-91 0.4031269995780953 1105 92-93 0.34006930662509305 1105 94-95 0.5622098178536845 1105 96-97 0.573187686880086 1105 98-99 0.4955056002929936 1105 100-101 0.48875552987240667 1106 1 0.07275781623902589 1106 2 0.026926832422535796 1106 3 0.14236510981852035 1106 4 0.1391204432556279 1106 5 0.22804977492214107 1106 6 0.1833620127569091 1106 7 0.3065397800520273 1106 8 0.35290854328088983 1106 9 0.3343625524708358 1106 10-11 0.3591947358923946 1106 12-13 0.1891136369818298 1106 14-15 0.292945431487162 1106 16-17 0.24369765303705293 1106 18-19 0.2648550809303458 1106 20-21 0.2935813801120446 1106 22-23 0.16937249027166246 1106 24-25 0.27006288292336933 1106 26-27 0.40090799538025834 1106 28-29 0.271191556027631 1106 30-31 0.45203401536457477 1106 32-33 0.6545921585103542 1106 34-35 0.5110397448412058 1106 36-37 0.48290610895384134 1106 38-39 0.4976448005287679 1106 40-41 0.5046131320699772 1106 42-43 0.4892909597438262 1106 44-45 0.6912142215283552 1106 46-47 0.9071769152872662 1106 48-49 0.9754041732828895 1106 50-51 0.7859361274991485 1106 52-53 0.6206814370784244 1106 54-55 0.5079290272710679 1106 56-57 0.704710964961567 1106 58-59 0.8374022822575817 1106 60-61 1.0780378822264645 1106 62-63 0.5090046251058062 1106 64-65 0.5480773055076611 1106 66-67 0.6852598767601954 1106 68-69 -2.7110291735999077 1106 70-71 -2.937630288969661 1106 72-73 0.911394418593229 1106 74-75 1.3946741651552301 1106 76-77 1.4247666816968199 1106 78-79 1.1899702723500702 1106 80-81 0.8660318605481798 1106 82-83 0.6895331137789924 1106 84-85 0.6554907107615442 1106 86-87 0.6309480424409522 1106 88-89 0.7600624974988044 1106 90-91 0.7579482005428098 1106 92-93 0.5591413616649668 1106 94-95 0.8501842974719693 1106 96-97 0.9639608321984277 1106 98-99 1.0412567836786835 1106 100-101 1.2413202429458003 1107 1 -0.07323870645497266 1107 2 0.1256899485767562 1107 3 0.13964427038465033 1107 4 -0.09182147963358744 1107 5 0.19874965901193775 1107 6 0.08922386609999933 1107 7 0.1430209903985329 1107 8 0.20306105670206875 1107 9 0.2773789629149519 1107 10-11 0.21589083764934713 1107 12-13 0.26907756755772283 1107 14-15 0.07235268807574613 1107 16-17 -0.035296292180127864 1107 18-19 0.17328640413678187 1107 20-21 0.30757829934093195 1107 22-23 0.396383379729933 1107 24-25 0.15650024566129161 1107 26-27 0.4898282766150075 1107 28-29 0.5060050321135279 1107 30-31 0.37756971875647594 1107 32-33 0.21002430528926652 1107 34-35 0.14296461672992677 1107 36-37 0.1984704848689205 1107 38-39 0.16879879333013292 1107 40-41 0.033712692831329605 1107 42-43 -0.03476803244748794 1107 44-45 0.2239969814112257 1107 46-47 0.17746882597540292 1107 48-49 0.2767024437806924 1107 50-51 0.2101255186655706 1107 52-53 0.030278344106243082 1107 54-55 0.13077210621201374 1107 56-57 0.4462750663524915 1107 58-59 0.6243368387485546 1107 60-61 0.5484800186344678 1107 62-63 0.10441397112825967 1107 64-65 0.01949209625924908 1107 66-67 0.09911877134291913 1107 68-69 -2.7797090174261676 1107 70-71 -3.011126125353261 1107 72-73 0.8729335840397887 1107 74-75 1.0639026760141874 1107 76-77 0.7943055573678777 1107 78-79 0.8032255950684721 1107 80-81 0.586015694125539 1107 82-83 0.5778494418658653 1107 84-85 0.32918748357755234 1107 86-87 0.3848790758718934 1107 88-89 0.5041442141898749 1107 90-91 0.20168333048424714 1107 92-93 0.5839381375312449 1107 94-95 0.9186898947145288 1107 96-97 0.618414224096913 1107 98-99 0.6008883875098228 1107 100-101 0.9726198098076821 1108 1 -0.005433473896832908 1108 2 -0.061123681914200034 1108 3 0.030923340152089196 1108 4 6.849803147304101E-4 1108 5 -0.029912324192196138 1108 6 -0.10646815586382274 1108 7 0.0157600084863887 1108 8 0.24314503603023496 1108 9 0.21453174069272762 1108 10-11 0.21224500431601 1108 12-13 0.217815858901389 1108 14-15 0.2627495711506853 1108 16-17 0.33673415032632903 1108 18-19 0.13032169160448603 1108 20-21 0.1402939001161343 1108 22-23 0.08233702960072975 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0.4709962383592412 2312 30-31 0.21467096132110441 2312 32-33 0.6842798746669843 2312 34-35 0.49941229746660554 2312 36-37 0.613104042693216 2312 38-39 0.6647502703948831 2312 40-41 0.210703084046159 2312 42-43 -0.1941959379611049 2312 44-45 0.6849291193659255 2312 46-47 0.7987833135068669 2312 48-49 0.6795066147370008 2312 50-51 0.9814324993336143 2312 52-53 0.9405924169728692 2312 54-55 1.009311592313594 2312 56-57 1.0173498704591033 2312 58-59 0.9211455281216949 2312 60-61 0.8522359526312648 2312 62-63 0.9335420310687788 2312 64-65 1.0935828721114547 2312 66-67 1.7206148534751513 2312 68-69 4.108480184129544 2312 70-71 4.874887694424817 2312 72-73 3.0157656018846772 2312 74-75 2.543830574378404 2312 76-77 2.5487054730050325 2312 78-79 1.9033987891316144 2312 80-81 1.7615611928672443 2312 82-83 1.7052704053181635 2312 84-85 1.7558200476361172 2312 86-87 1.3868719693745248 2312 88-89 1.563090608035676 2312 90-91 1.8536960635544837 2312 92-93 2.100212838686584 2312 94-95 2.084329014767146 2312 96-97 2.2266217281005716 2312 98-99 2.1027145214036693 2312 100-101 2.107091684092744 2313 1 -0.3457673557651617 2313 2 -0.24888031177037107 2313 3 -0.30240067843600116 2313 4 -0.041265182348318774 2313 5 -0.05538849741885343 2313 6 -0.033685151478991315 2313 7 -2.6860177278925192 2313 8 -3.284761595723385 2313 9 -1.0796679329461938 2313 10-11 -0.08946839132261886 2313 12-13 -0.07639312948278132 2313 14-15 -0.674615839405341 2313 16-17 -0.33916360137016 2313 18-19 0.1080525506654908 2313 20-21 -2.5889538824570195 2313 22-23 -0.5947012609020206 2313 24-25 -0.022547860146453047 2313 26-27 -1.325139944902574 2313 28-29 -1.4949650112776638 2313 30-31 -1.014356669205256 2313 32-33 -1.0485040797797183 2313 34-35 -1.6113070510493301 2313 36-37 -2.267969142968198 2313 38-39 -2.1672767379337756 2313 40-41 -1.3800055982234056 2313 42-43 -0.4512886710767745 2313 44-45 -0.3073573243297645 2313 46-47 -0.6311857690401652 2313 48-49 -2.670992747029274 2313 50-51 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0.5708559859668405 2314 74-75 0.29516109967786974 2314 76-77 0.25303551980192296 2314 78-79 -2.1451619006300824 2314 80-81 -1.601648477702863 2314 82-83 -0.3414258721396415 2314 84-85 -0.03918434769108714 2314 86-87 -0.3243736913912407 2314 88-89 -0.5416837423368541 2314 90-91 -0.12290785298673157 2314 92-93 -0.560397755162036 2314 94-95 -0.12337841940568595 2314 96-97 0.14222128993770156 2314 98-99 0.061683043680965 2314 100-101 0.19566874591688332 2315 1 -0.3198419673245354 2315 2 -0.32689157316237427 2315 3 -0.5201383383110034 2315 4 -0.08457510282199365 2315 5 -0.24212443519095928 2315 6 -0.21736580781303871 2315 7 -1.0352398791666815 2315 8 -2.7043664795298135 2315 9 -0.6929561820125798 2315 10-11 -1.3254361551042777 2315 12-13 -0.7052770520075029 2315 14-15 -0.42623200395323835 2315 16-17 -0.24916631029606862 2315 18-19 -0.02749484586321671 2315 20-21 -0.5822541281952951 2315 22-23 -0.11016100432804166 2315 24-25 -4.926844410135811 2315 26-27 -0.6147120313560066 2315 28-29 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-1.019220829860064 2315 94-95 0.1695617244246712 2315 96-97 0.29776205018387003 2315 98-99 -1.8784707610409015 2315 100-101 -3.6725115307720273 2316 1 -0.49253368437991796 2316 2 -0.3064913428139704 2316 3 -0.7902122417566311 2316 4 -0.3699119873598562 2316 5 -0.2751362405465585 2316 6 -0.5914708082929323 2316 7 -0.7066478837736483 2316 8 -1.0626398263001349 2316 9 -4.763246037085047 2316 10-11 -1.7410938477811229 2316 12-13 -0.8408682795725184 2316 14-15 -0.4020178279286668 2316 16-17 -0.46315397705871675 2316 18-19 -0.33064054122746 2316 20-21 -3.885078295581799 2316 22-23 -1.2345527405791188 2316 24-25 -0.42857322288175936 2316 26-27 -0.4500189226123652 2316 28-29 -0.07755250099905453 2316 30-31 -0.23849160850621587 2316 32-33 -1.4375526124811557 2316 34-35 -0.8550934682590352 2316 36-37 -0.3381344406277691 2316 38-39 -5.473055856228363 2316 40-41 -5.251469426373966 2316 42-43 -3.9165630103724354 2316 44-45 -2.4958912636218855 2316 46-47 -2.485231229212232 2316 48-49 -3.0031281991552916 2316 50-51 -1.3034126987737196 2316 52-53 -1.0497885709047914 2316 54-55 -0.20706419345685845 2316 56-57 -3.371355659364948 2316 58-59 -0.43580561434195175 2316 60-61 -0.34239677496376686 2316 62-63 0.01276110137108688 2316 64-65 0.2946145438309955 2316 66-67 0.09013008480870255 2316 68-69 3.1527137198806763 2316 70-71 3.9339673487085456 2316 72-73 2.374630603907338 2316 74-75 0.8266596434534819 2316 76-77 -1.607131045833004 2316 78-79 0.09936073579694593 2316 80-81 -0.10219484671331358 2316 82-83 0.4036203441837465 2316 84-85 0.45631565686673525 2316 86-87 0.31184168624717046 2316 88-89 0.12320119542607699 2316 90-91 0.11210517099197048 2316 92-93 0.1245441667806908 2316 94-95 0.290263087319385 2316 96-97 0.6396338708960307 2316 98-99 0.9275146982162212 2316 100-101 0.9703812612425651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 31.0 4 89.0 5 189.0 6 510.0 7 1109.0 8 2248.0 9 3788.0 10 5950.0 11 8572.0 12 11096.0 13 13458.0 14 17131.0 15 21218.0 16 25275.0 17 28039.0 18 25235.0 19 21909.0 20 21405.0 21 21846.0 22 22888.0 23 25113.0 24 30215.0 25 37076.0 26 45764.0 27 56296.0 28 68464.0 29 81141.0 30 94292.0 31 109014.0 32 125774.0 33 143779.0 34 164432.0 35 181231.0 36 178822.0 37 142193.0 38 56188.0 39 2734.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.817002207210216 21.57074220230531 16.088444474171183 21.52381111631329 2 15.61177860030331 22.45626609698011 39.05856806615528 22.873387236561303 3 18.60345241684225 27.018112455147946 31.239819650032796 23.13861547797701 4 12.602659813569892 18.706589524711415 37.40760198498877 31.283148676729922 5 12.318356415681768 39.6001263849827 34.03768256305179 14.043834636283748 6 29.759372577690822 38.12674942616871 17.46826583420497 14.645612161935496 7 26.822203411837283 34.797831394185735 20.976340708948424 17.403624485028562 8 26.329034497862096 35.18528941213708 19.638041879792727 18.847634210208096 9 26.31075659912946 17.01973288634212 20.982247590855923 35.6872629236725 10-11 24.394516217663735 28.086606041657262 26.97080045856093 20.548077282118076 12-13 25.643000317076964 25.810148357468886 27.896642940691002 20.65020838476314 14-15 22.477747494172526 26.68002030629969 27.159257895021334 23.68297430450645 16-17 20.543827606815817 30.12860110253399 27.70380648675608 21.623764803894115 18-19 20.299150716893465 27.793187401487923 31.4372669807165 20.47039490090211 20-21 21.45577333622362 26.775979274646044 31.441190024948217 20.327057364182117 22-23 21.128008889304972 26.977970661727174 31.347198910458307 20.546821538509548 24-25 20.920358460210437 27.20681455574484 31.574190017793725 20.298636966250996 26-27 20.74978196722682 27.401160798125336 31.42987703787501 20.41918019677284 28-29 20.28092559667851 27.49027373416443 31.796356248117508 20.43244442103955 30-31 20.54794253371249 27.18570442231402 31.64911594399149 20.617237099982 32-33 20.526033934725138 27.33524138146251 31.360420483877437 20.77830419993491 34-35 20.966373910523618 27.289154276543098 31.307093020663828 20.437378792269453 36-37 21.18784165234662 26.783117793613958 31.290795402637343 20.738245151402083 38-39 20.713806415338716 27.330673801886103 31.42001018520223 20.535509597572954 40-41 20.687816686320936 27.335318930365332 31.466786609294235 20.5100777740195 42-43 20.741535804208404 27.324298797244968 30.94335581788932 20.990809580657313 44-45 20.673911346093785 27.56519653772364 30.702466202650246 21.058425913532325 46-47 21.132645907821626 27.7115969453456 30.187605928835897 20.968151217996876 48-49 21.047687349532307 27.88877059981853 29.92272297102488 21.140819079624286 50-51 20.9712008650643 28.029488699149518 29.65285056588601 21.346459869900173 52-53 21.26177408805705 28.212647014052795 29.441590542479272 21.08398835541088 54-55 20.837953910750795 28.052354835814086 29.595028342329172 21.514662911105944 56-57 21.17535839314565 28.37471492008565 29.172646040486296 21.2772806462824 58-59 21.371192942207298 27.80661300193634 29.296494848119004 21.525699207737354 60-61 21.203953805207295 28.02491150308078 29.73382233402247 21.03731235768946 62-63 21.133719723361754 27.97472350525098 29.785143107435136 21.10641366395213 64-65 20.953152664145122 27.975934057176676 29.531771848352907 21.539141430325294 66-67 20.867494936110432 28.230856432242113 29.374075899457864 21.527572732189594 68-69 21.214906309214683 27.628773949049055 29.860060355112637 21.29625938662362 70-71 20.92053268216363 27.600542928863327 30.238083923394953 21.24084046557809 72-73 21.155530548026576 27.88779164005267 29.389576409498037 21.567101402422722 74-75 20.85130892008204 28.43761752374729 28.95489625014044 21.756177306030235 76-77 20.262551962187693 28.43430658870349 29.55444788509115 21.748693564017664 78-79 20.914799001038308 28.219497364656128 28.9888383141156 21.87686532018996 80-81 20.735569474939535 28.633739397924725 28.82527334128373 21.80541778585201 82-83 20.9971810908791 28.46175943729926 28.850365995658372 21.690693476163265 84-85 20.703473175554002 28.47812815806873 28.62474195609332 22.193656710283946 86-87 20.800682095549103 28.234130411765037 28.856319693279907 22.108867799405953 88-89 20.82503836004303 28.20298348769762 28.66202231750664 22.30995583475271 90-91 20.685903155140025 28.267946993959665 28.85537357956841 22.1907762713319 92-93 20.9079018788097 28.27618625148654 28.652572343145525 22.163339526558236 94-95 20.533008675361454 27.884353424705665 28.746675039659713 22.835962860273167 96-97 20.659526768763694 27.815108178567876 28.807155023939185 22.71821002872925 98-99 20.568345091185122 27.95118550133704 28.46501792937709 23.015451478100747 100-101 20.261264153340957 27.72901159019014 28.945045243875523 23.06467901259338 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4385.0 1 2976.5 2 1544.0 3 1582.0 4 1544.5 5 1507.5 6 2185.0 7 3965.0 8 5641.5 9 6347.0 10 5810.0 11 4624.0 12 4070.5 13 3948.5 14 3924.0 15 4016.0 16 4166.5 17 4327.5 18 4527.5 19 4775.0 20 5196.5 21 5819.5 22 6515.0 23 7436.0 24 8499.5 25 10133.0 26 12720.0 27 15224.5 28 17131.5 29 19678.5 30 23327.5 31 27613.0 32 32454.0 33 37343.0 34 42288.5 35 46892.0 36 52730.0 37 57728.5 38 58637.0 39 58330.0 40 58778.0 41 60167.5 42 61567.5 43 62875.5 44 63891.5 45 64453.5 46 63339.5 47 63076.0 48 66892.0 49 65863.0 50 59159.0 51 53723.5 52 49939.5 53 48508.0 54 46972.5 55 43230.5 56 39148.0 57 35039.5 58 31571.5 59 28297.0 60 24172.0 61 19851.0 62 15732.5 63 12026.5 64 9082.5 65 6972.0 66 5216.5 67 3989.5 68 2985.0 69 2032.5 70 1501.0 71 1222.0 72 1008.0 73 863.0 74 691.5 75 502.0 76 332.0 77 194.0 78 111.5 79 69.0 80 38.0 81 21.5 82 15.5 83 11.5 84 6.0 85 2.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022122944502615465 2 0.017497744518441452 3 3.3435180608486854E-4 4 0.08130321417963719 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01159086261094211 12-13 0.0 14-15 0.0 16-17 8.080168647050989E-4 18-19 1.9503855354950663E-4 20-21 0.0 22-23 5.5725301014144755E-5 24-25 0.0035664192649052643 26-27 0.0034549686628769748 28-29 0.0011423686707899676 30-31 2.2290120405657902E-4 32-33 7.244289131838819E-4 34-35 0.004290848178089146 36-37 0.08782307439829214 38-39 0.09584751774432898 40-41 0.08010512020783309 42-43 0.08406161657983736 44-45 0.0823062695978918 46-47 0.08080168647050989 48-49 0.07567495877720858 50-51 0.0755356455246732 52-53 0.059403170881078306 54-55 0.05071002392287172 56-57 0.02800196375960774 58-59 0.023125999920870072 60-61 0.013680561398972536 62-63 0.002424050594115297 64-65 0.006352684315612501 66-67 0.16216062595116124 68-69 0.7462732311814265 70-71 0.9129754691652405 72-73 0.36839996500451094 74-75 0.055530262460595246 76-77 0.20459544267343246 78-79 0.014154226457592766 80-81 0.0031763421578062507 82-83 0.0015881710789031253 84-85 3.6221445659194093E-4 86-87 0.0026190891476648034 88-89 3.6221445659194093E-4 90-91 0.00445802408113158 92-93 0.004625199984174015 94-95 0.08082954912101696 96-97 0.0390355733604084 98-99 0.014209951758606914 100-101 0.013429797544408886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1794517.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.67879070580224 #Duplication Level Percentage of deduplicated Percentage of total 1 72.60273548965618 32.43802423611069 2 13.798494061817127 12.330000564863647 3 5.26666719609356 7.059249641141355 4 2.609867420828513 4.664228810603561 5 1.462000497348523 3.2660207116406723 6 0.919555013300347 2.465076358903044 7 0.6003376548348853 1.877565230322599 8 0.436203892570345 1.5591249936965348 9 0.31541298093330555 1.2683043504911113 >10 1.694412694113904 14.249833225051697 >50 0.17773768456495095 5.516236649589166 >100 0.11086114893879966 8.975657019178565 >500 0.004063477333027388 1.1880827485491998 >1k 0.0011428529999139528 0.882055417205215 >5k 2.5396733331421175E-4 0.8381427078132528 >10k+ 2.5396733331421175E-4 1.4223973348396441 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13357 0.7443228456459314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11714 0.6527661760796917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8951 0.4987971693776097 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5822 0.32443270250435075 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2759 0.1537461054980254 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2716 0.15134991755441715 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2217 0.12354299234835892 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1901 0.10593379722788918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 5.5725301014144755E-5 0.0 2.7862650507072377E-5 0.0 12-13 0.0 1.1145060202828951E-4 0.0 5.5725301014144755E-5 0.0 14-15 0.0 1.1145060202828951E-4 0.0 5.5725301014144755E-5 0.0 16-17 0.0 1.1145060202828951E-4 0.0 1.1145060202828951E-4 0.0 18-19 0.0 1.1145060202828951E-4 0.0 1.6717590304243427E-4 0.0 20-21 0.0 1.1145060202828951E-4 0.0 2.2290120405657902E-4 0.0 22-23 0.0 1.1145060202828951E-4 0.0 5.572530101414475E-4 0.0 24-25 0.0 1.1145060202828951E-4 0.0 0.001114506020282895 0.0 26-27 0.0 1.1145060202828951E-4 0.0 0.0020896987880304283 0.0 28-29 0.0 1.6717590304243427E-4 0.0 0.0045416120326527976 0.0 30-31 0.0 1.9503855354950666E-4 0.0 0.010810708396744083 0.0 32-33 0.0 2.2290120405657902E-4 0.0 0.021649279443995237 0.0 34-35 0.0 2.2290120405657902E-4 0.0 0.037726028786575994 0.0 36-37 0.0 2.2290120405657902E-4 0.0 0.06260737568939163 0.0 38-39 0.0 2.2290120405657902E-4 0.0 0.10345402133275974 0.0 40-41 0.0 2.2290120405657902E-4 0.0 0.1495945705724716 0.0 42-43 0.0 2.2290120405657902E-4 0.0 0.19350610777161764 0.0 44-45 0.0 2.2290120405657902E-4 0.0 0.2458043027733925 0.0 46-47 0.0 2.2290120405657902E-4 0.0 0.31273038929138036 0.0 48-49 0.0 2.507638545636514E-4 0.0 0.38592557217345946 0.0 50-51 0.0 2.7862650507072377E-4 0.0 0.4676745887612098 0.0 52-53 0.0 2.7862650507072377E-4 0.0 0.5515411667874976 0.0 54-55 0.0 2.7862650507072377E-4 0.0 0.6394756917878182 0.0 56-57 0.0 2.7862650507072377E-4 0.0 0.74376559263579 0.0 58-59 0.0 2.7862650507072377E-4 0.0 0.8463558718028306 0.0 60-61 0.0 2.7862650507072377E-4 0.0 0.9554660111885259 0.0 62-63 0.0 2.7862650507072377E-4 0.0 1.0558272783150007 0.0 64-65 2.7862650507072377E-5 3.0648915557779616E-4 0.0 1.161816800843904 0.0 66-67 5.5725301014144755E-5 3.3435180608486854E-4 0.0 1.273852518532842 0.0 68-69 5.5725301014144755E-5 3.3435180608486854E-4 0.0 1.4094879012012704 0.0 70-71 5.5725301014144755E-5 3.3435180608486854E-4 0.0 1.581456180130921 0.0 72-73 5.5725301014144755E-5 3.3435180608486854E-4 0.0 1.7749622879025386 0.0 74-75 5.5725301014144755E-5 3.3435180608486854E-4 0.0 1.9799478076830703 0.0 76-77 1.1145060202828951E-4 3.3435180608486854E-4 0.0 2.211347120144306 0.0 78-79 1.1145060202828951E-4 3.3435180608486854E-4 0.0 2.47122206142377 0.0 80-81 1.1145060202828951E-4 3.3435180608486854E-4 0.0 2.7760951832721563 0.0 82-83 1.1145060202828951E-4 3.3435180608486854E-4 0.0 3.0815812834316976 0.0 84-85 1.1145060202828951E-4 3.3435180608486854E-4 0.0 3.3773711812147784 0.0 86-87 1.1145060202828951E-4 3.3435180608486854E-4 0.0 3.691494703031512 0.0 88-89 1.1145060202828951E-4 3.3435180608486854E-4 0.0 4.031419039217795 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8975 0.0 35.75778 1 GTATCAA 13525 0.0 31.310204 1 ATCAACG 17400 0.0 24.077719 3 GTACATG 11440 0.0 24.069122 1 TATCAAC 17550 0.0 23.930668 2 TCAACGC 17615 0.0 23.789145 4 CAACGCA 17835 0.0 23.500675 5 TACATGG 11550 0.0 23.419712 2 AACGCAG 18290 0.0 22.89013 6 ACATGGG 11770 0.0 21.96969 3 GAGTACT 10685 0.0 21.671772 12-13 ATAGCGT 155 3.963363E-5 21.412508 6 AGTACTT 11255 0.0 21.037512 12-13 GTACTTT 11335 0.0 20.554476 14-15 AACGCAT 190 0.0 20.222221 64-65 ACGCAGA 20740 0.0 20.09469 7 AGAGTAC 17815 0.0 19.998432 10-11 CATGGGA 7745 0.0 19.97547 4 CGCAGAG 20945 0.0 19.92065 8 GTATAGG 620 0.0 19.908566 1 >>END_MODULE