FastQCFastQC Report
Fri 27 May 2016
SRR939399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432128
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131910.5423645466028104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.42222284353455086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69710.28662142781958844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34100.1402064365033419No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.10188608494289773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116600.034.5439151
GTATCAA185800.034.251651
TATCAAC231950.027.460142
ATCAACG233450.026.9765573
TCAACGC234400.026.707734
CAACGCA236000.026.173135
GTACATG145450.026.065221
AACGCAG238750.025.7868126
TACATGG151800.024.5755372
ACATGGG155150.023.4023673
ACGCAGA260750.023.177487
GAGTACT124800.023.111712-13
CGCAGAG259200.023.0030638
AACGCCG2404.483809E-922.3065246
AGTACTT131550.021.88935912-13
GCAGAGT255300.021.8773529
CAACGCG3550.021.817685
GTACTTT139200.021.0533514-15
CATTCCG5850.021.0482839
AACGCGG3700.021.045966
Produced by FastQC (version 0.11.5)