FastQCFastQC Report
Fri 27 May 2016
SRR939399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432128
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187190.7696552155149728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157830.6489378848481658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113160.4652715646544918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49870.2050467738540077No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39360.16183358770591022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30830.1267614204515552No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25100.10320180516814904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135600.034.9932751
GTATCAA204100.030.4023281
CATTCCG5700.029.9254979
GTACATG164600.024.2777231
ATCAACG254950.024.216043
TCAACGC256400.024.0606144
TATCAAC260000.023.8229922
CAACGCA259000.023.7641965
AACGCAG265100.023.2531226
TACATGG171650.022.9706652
GAGTACT151550.022.19857412-13
ACATGGG167550.022.1144453
GGACCGA1100.00292010721.5372896
AGTACTT157500.021.02903212-13
ACGCAGA297400.020.6957867
CGCAGAG296800.020.689738
GTACTTT164000.020.36891714-15
CCTTTAG9600.020.2452351
TAGGACG3302.910383E-1120.1014694
CATGGGA115250.019.9395144
Produced by FastQC (version 0.11.5)