FastQCFastQC Report
Fri 27 May 2016
SRR939398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2433007
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133600.5491147374421858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96180.39531328927536996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72170.2966288218652885No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34380.14130662180585588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA181550.035.8437961
GGTATCA114750.034.749041
ATCGACG1852.4738256E-1028.638743
GTATCGA1803.416062E-928.1123241
ATCAACG232600.027.975553
TCAACGC234450.027.826094
TATCAAC228100.027.8092822
GTACATG144600.027.4707581
CAACGCA234200.027.4114915
AACGCAG240150.026.8866886
CATTCCG5750.026.192669
TACATGG150700.025.5548292
TCGACGC2301.2914825E-1024.8811114
ACGCAGA260400.024.347557
CAACGCG3600.024.3173125
ACATGGG157350.024.2738913
CGCAGAG260700.024.208238
GAGTACT125800.024.14167212-13
GCAGAGT255900.023.0480469
AACGCGG3800.023.0135616
Produced by FastQC (version 0.11.5)